Labshake search
Citations for New England Biolabs :
201 - 250 of 1435 citations for Anti Myc magnetic beads since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2020Quote: ... captured messenger RNA was isolated from the lysate by adding 20 µL of LBB-washed streptavidin-coated magnetic beads (New England Biolabs) and was allowed to stand for 10 min ...
-
bioRxiv - Cell Biology 2022Quote: ... Total RNA (3 μg) was applied for poly(A) mRNA purification by using oligo-d(T) magnetic beads (S1419S, NEB). RNA fragmentation ...
-
bioRxiv - Synthetic Biology 2019Quote: Immobilized toeholds strands bound on the magnetic beads were mixed with 30 µL of in vitro transcription buffer (NEB, E2050) containing 2 µL of T7 RNA Polymerase Mix and ATP ...
-
bioRxiv - Molecular Biology 2019Quote: ... and poly(A) RNA was isolated using one round of selection with oligo(dT)25 magnetic beads (New England Biolabs). RNA was then subjected to fragmentation using RNA Fragmentation Reagents (ThermoFisher ...
-
bioRxiv - Immunology 2021Quote: ... IDEZ was used to cleave the Fab (in the supernatant) from the Fc (remained on the magnetic beads) (New England Biolabs) for 1 h at 37°C and collected ...
-
bioRxiv - Plant Biology 2020Quote: ... The supernatant was collected after a spin at 16000 g for 30 min and incubated with GFP-Trap (Chromtek) or MBP magnetic beads (NEB) for 3 h at 4°C ...
-
bioRxiv - Molecular Biology 2021Quote: Total RNA was isolated from HEK293T cells using Bio Tri RNA reagent (Bio-lab) and mRNA was captured using Oligo d(T)25 magnetic Beads (NEB) according to manufacturer’s protocol ...
-
bioRxiv - Plant Biology 2021Quote: ... captured messenger RNA was isolated from the lysate by adding 20 μl of LBB-washed streptavidin-coated magnetic beads (New England Biolabs) and incubated for 10 min at room temperature ...
-
bioRxiv - Microbiology 2020Quote: ... 20 μg of recombinant PbChiB2 and PbChiB4 resuspended in 20 mM Tris (pH 8.0) were independently mixed with 2 mg of chitin magnetic beads (NEB, CAD), chitin from shrimp shell (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2021Quote: ... Enrichment of mRNA from eukaryotic organisms was performed using oligo(dT) beads from NEBNext Poly(A) mRNA Magnetic Isolation Module (Cat.No. E7490L, NEB, USA). Subsequently ...
-
bioRxiv - Immunology 2021Quote: ... a flow-focusing device was used to encapsulate individual T cells into emulsification droplets containing lysis buffer and oligo(dT) magnetic beads (New England Biolabs)30 ...
-
bioRxiv - Microbiology 2020Quote: ... Circularised product is subjected to two rounds of rRNA depletion using biotinylated oligos [17] and streptavidin-coated magnetic beads (NEB) according to manufacturer’s protocol ...
-
bioRxiv - Synthetic Biology 2020Quote: 10 ng of DNA oligo out of the master pool was attached to 1 μL of Streptavidin Magnetic Beads (NEB). The iDR reaction mixtures contained 1 μL of the DNA template attached to the beads (10 ng/μL) ...
-
bioRxiv - Plant Biology 2021Quote: ... The solution was split into two halves and mRNAs were isolated from each using 150 μL magnetic Oligo(dT)25 beads (NEB) according to the manufacturer’s protocol ...
-
bioRxiv - Genomics 2019Quote: ... Biotinylated DNA was pulled down using streptavidin coated magnetic beads and prepared for multiplex sequencing on an Illumina platform using the NEBNext Ultra II kit (NEB). All end preparation ...
-
bioRxiv - Immunology 2021Quote: For RNA-seq library preparation 2ug of total RNA was used to isolate mRNA using magnetic beads with mRNA isolation kit (PolyA mRNA isolation module, NEB). Later mRNA library preparation kit ...
-
bioRxiv - Biochemistry 2022Quote: ... The supernatant was then transferred to a fresh low-bind tube containing pre-washed 5 µl amylose magnetic beads (NEB) and rotated for 45 min at 4 °C ...
-
bioRxiv - Molecular Biology 2022Quote: ... PolyA + fraction was isolated from 4.5 μg of DNAse-treated total RNA using NEBNext Oligo d(T)25 Magnetic beads kit (NEB, USA), according to manufacturer’s recommendations ...
-
bioRxiv - Microbiology 2022Quote: ... Proteins from the supernatant at 2 mg/mL were used for the Co-IP assay using Protein G magnetic beads (New England Biolabs) and a mouse monoclonal anti-HA antibody (Sigma) ...
-
bioRxiv - Molecular Biology 2022Quote: ... 25 μg of total RNAs from 12-day-old Arabidopsis leaves were extracted using the Trizol method and incubated with 30 μl of pre-equilibrated oligo d(T)25 magnetic beads (NEB) for 15 minutes at room temperature with continuous rotation ...
-
bioRxiv - Molecular Biology 2022Quote: ... and mRNA fragments with a poly(A) tail were isolated by Oligo d(T)25 Magnetic Beads (NEB Biolab, S1419S). Illumina sequence adaptors were added during reverse transcription with SMRT methods (Wang et al. ...
-
bioRxiv - Microbiology 2023Quote: ... and eukaryotic mRNA was removed by binding and discarding the eukaryotic mRNA polyA region to oligo d(T)25 magnetic beads (England Biolabs). Finally ...
-
bioRxiv - Immunology 2023Quote: ... mRNAs were enriched by incubation with Oligo d(T) Magnetic Beads (New England Biolabs, Cat. No. S1419S, Ipswich, MA, USA) and then fragmented/eluted by incubation at 94°C for 9 min ...
-
bioRxiv - Molecular Biology 2023Quote: Equilibration buffer (GF150 buffer supplemented with 0.1 mM Mg-ATP and 10% glycerol) was used to equilibrate 20 μL of SNAP-Capture Magnetic Beads (New England Biolabs) slurry in 1.5 mL Protein Lo Bind Tubes (Eppendorf) ...
-
Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... with prior enrichment of mRNA by using Oligo dT beads from the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB). After library preparation ...
-
bioRxiv - Molecular Biology 2022Quote: Polyadenylated RNA was purified from the DNase-treated total RNA using the oligo d(T)25 magnetic beads (NEB, S1419) and used for the library preparation ...
-
bioRxiv - Molecular Biology 2023Quote: ... was isolated from total RNA or crude cell lysates by 2 rounds of affinity chromatography using oligo d(T)25-derivatized magnetic beads (NEB). RNA yields were quantified by ultraviolet (UV ...
-
bioRxiv - Biochemistry 2023Quote: ... The duplex hRNase 4 cleavage products (5′ fragments of the RNA) were enriched using Hydrophilic Streptavidin Magnetic Beads (New England Biolabs). The beads were washed twice by a high salt buffer (5 mM Tris HCl ...
-
bioRxiv - Biophysics 2023Quote: Magnetic tweezers force calibration was performed as described previously70 using transverse fluctuations of a magnetic bead tethered to a glass coverslip via lambda phage DNA (New England Biolabs).
-
bioRxiv - Biophysics 2023Quote: ... To enrich for fully assembled TC-TL complexes the expressome was purified via immobilizing the 50S subunit (ZS22) onto streptavidin magnetic beads (NEB). For this ...
-
bioRxiv - Biochemistry 2024Quote: ... 4SU-labeled RNA (100 µg) was biotinylated with HPDP-biotin (cat. 21341, ThermoScientific) and captured on 150 µL of streptavidin magnetic beads (NEB) per RNA sample ...
-
bioRxiv - Microbiology 2024Quote: ... Polyadenylated RNA was then isolated from the whole-cell RNA using NEB Oligo d(T)25 Magnetic beads (NEB, S1419S) according to the manufacturers protocol ...
-
bioRxiv - Cell Biology 2021Quote: ... the myc tag was removed from the Ccz1-myc plasmid using NEB site-directed mutagenesis (SDM) kit (NEB) following manufacturer’s instructions and primers selected using NEBaseChanger tool (table 1) ...
-
bioRxiv - Microbiology 2019Quote: ... and 1 ml of lysate (corresponding to 50 ml of the original culture) was transferred into tubes containing 50 μl of pre-washed (3x) magnetic α-HA beads (NEB) and incubated for 2 h at 4°C ...
-
bioRxiv - Cell Biology 2021Quote: ... 500ng-1 μg total RNA was enriched in poly-A tailed RNA transcripts by double incubation with Oligo d(T) Magnetic Beads (NEB, S1419S) and fragmented for 9 min at 94°C in 2X Superscript III first-strand buffer containing 10mM DTT (Invitrogen ...
-
bioRxiv - Cell Biology 2021Quote: ... 50 μg of biotinylated RNA was subjected to oligo d(T) selection using oligo d(T)25 magnetic beads (NEB S1419S), substituting SDS and NaCl for the recommended LiDS and LiCl ...
-
bioRxiv - Genomics 2022Quote: ... Biotinylated DNA was pulled down using streptavidin coated magnetic beads and prepared for multiplex sequencing on an Illumina platform using the NEBNext Ultra II kit (NEB, E7645S). All end preparation ...
-
bioRxiv - Cancer Biology 2022Quote: ... 500 ng total RNA was enriched for poly-A tailed RNA transcripts by double incubation with Oligo d(T) Magnetic Beads (NEB, S1419S) and fragmented for 9 min at 94°C in 2X Superscript III first-strand buffer containing 10 mM DTT (Invitrogen ...
-
bioRxiv - Cell Biology 2019Quote: ... 400 µL of lysate at the protein concentration of 1,5 µg/µL were added to 100 μL of washed Oligo d(T)25 Magnetic Beads (NEB, Ipswich, MA) and incubated at RT for 30 min ...
-
bioRxiv - Molecular Biology 2022Quote: ... depletion was achieved by taking the unbound supernatant after chRNA was incubated with the oligo-d(T) beads from the NEBNext Poly(A) mRNA magnetic isolation module (NEB, E7490L). Then ...
-
bioRxiv - Plant Biology 2022Quote: ... PolyA RNA was then enriched from 1 μg of DNase I-treated RNA using Oligo d(T)25 Magnetic Beads (NEB S1419S), followed by RNA-seq libraries construction using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB E7420) ...
-
bioRxiv - Microbiology 2023Quote: ... The RNA was subjected to 3’ adaptor ligation using the same procedure as described above and subsequently enriched with Hydrophilic Streptavidin Magnetic Beads (New England BioLabs, S1421). After washing thoroughly ...
-
bioRxiv - Developmental Biology 2024Quote: ... Poly(A)-tailed RNAs were enriched and purified using oligo-dT beads from the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, USA). These samples then underwent fragmentation ...
-
bioRxiv - Molecular Biology 2021Quote: Myc-Tag (9B11) antibody (NEB 2276 S) and mouse IgG (Santa Cruz sc-2025 ...
-
bioRxiv - Pathology 2022Quote: ... Total RNA was subjected to two rounds of poly(A) selection using oligo-d(T)25 magnetic beads (New England Biolabs, CA, USA). Illumina compatible RNAseq library was prepared using NEB next ultra RNAseq library preparation kit ...
-
bioRxiv - Biophysics 2021Quote: ... PNGase F was then removed by incubating the samples in chitin magnetic beads according to manufacturer instructions (New England Biolabs, Ipswich, MA). Deglycosylation of proteins was confirmed via sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ...
-
bioRxiv - Cancer Biology 2023Quote: ... mRNA was extracted from homogenized cell lysate by 4 × 250 µL/brain Oligo (dT)25 magnetic beads (New England Biolabs, Cat: S1419S), and immunoprecipitated (IP ...
-
bioRxiv - Molecular Biology 2024Quote: ... Samples were normalized to an input of 150 ng before preparation with a NEBNext® Ultra™ II RNA Library Prep (Magnetic Bead) kit (New England Biolabs). The polyA mRNA protocol was used for all preps ...
-
bioRxiv - Cell Biology 2024Quote: ... A minimum of 75 µg of total RNA was used as starting material to purify poly(A) RNAs using Oligo d(T)25 Magnetic Beads (NEB, Cat # s1419S). 50 pmoles of linker (REL5 ...
-
bioRxiv - Biochemistry 2020Quote: ... A pre-cleaning step was applied by incubating each sample with 10 μl of Protein A magnetic beads (New England Biolabs GmbH, Frankfurt, Germany) for 1 h at 4 °C in tube roller (15 rpm ...