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Citations for New England Biolabs :
2651 - 2700 of 7437 citations for rno mir 155 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: Double-stranded DNA libraries were prepared with NEBNext Ultra II DNA Library Prep Kit for Illumina and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) (New England Biolab: NEB). Around 1 ng of DNA was used for each library ...
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bioRxiv - Genetics 2024Quote: ... was generated by amplifying the transgene rgef-1p::PH::miniSOG::unc-54 3’UTR from pNMS03 with primers P37 and P38 containing AvrII restriction sites and cloning the fragment into pCFJ151 after AvrII (New England BioLabs) digestion ...
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bioRxiv - Genomics 2024Quote: ... followed by a nested PCR reaction (5 cycles for 70 sec at 72◦C) with individual sample-barcoded i5 and i7 Illumina TruSeq primers (New England Biolabs NEBNext Multiplex Oligos for Illumina ...
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bioRxiv - Genomics 2024Quote: ... The backbones for the Gibson reaction for GRB2-SH3 and PSD95-PDZ3 library assembly (aPCA plasmids) were first linearized using primers listed in Extended Data Table 3 and next treated with Dpn1 (NEB) restriction enzyme to remove the circular plasmid template ...
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bioRxiv - Cell Biology 2024Quote: ... Size-selected DNA fragments were amplified using NEB unique multiplexed i5 and i7 primers (E6440) in a total reaction volume of 100 µl together with 2 U Phusion High-Fidelity DNA Polymerase (NEB) and in the presence of 0.2 mM dNTPs and 1X Phusion High-Fidelity buffer ...
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bioRxiv - Genomics 2024Quote: ... The ATAC libraries were amplified for 11 cycles using NEBNext 2X MasterMix and Nextera Index primers (New England Biolabs, # M0541S). The amplified libraries were size selected using AMPure beads (Beckman Coulter ...
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bioRxiv - Microbiology 2024Quote: ... and the colonies were screened for the insert of a right size with the primers pMRB-PlacGFP_ins_1_F and pMRB-PlacGFP_ins_1_R using Phusion polymerase (NEB, Phusion High Fidelity DNA Pol, M0530L) polymerase for the insert longer than 5kb (PCR thermocycling conditions were as follows ...
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bioRxiv - Microbiology 2024Quote: ... The colonies were screened for insert using Check1_ins_pSW_for and Check1_ins_pSW_rev primers from Supplementary Data 7 using Phusion polymerase (NEB, Phusion High Fidelity DNA Pol, M0530L) polymerase for inserts >5kb (PCR thermocycling conditions were as follows ...
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bioRxiv - Microbiology 2024Quote: ... Approximately 400 ng of modified or control RNA was incubated with of 200 ng/µL random nonamer primer (NEB; S1254S) and 1.0 µL of POWV_SHAPEMAP_RT_Primer (Supplemental Table S2 ...
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bioRxiv - Neuroscience 2024Quote: ... The UNC13A CE was amplified with a forward primer in exon 19 5’-CAGACGATCATTGAGGTGCG-3’and reverse primer in exon 22 5’-ATACTTGGAGGAGAGGCAGG-3’using Q5 High Fidelity Master Mix (NEB). PCR products were resolved on a TapeStation 4200 (Agilent ...
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bioRxiv - Molecular Biology 2024Quote: ... The NEBuilder Assembly Tool was used to design primers for amplification of each component of the donor plasmid (Table 2) using Phusion (NEB); purified PCR products were assembled using NEBuilder HiFi DNA Assembly Master Mix (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... mutagenic primers were designed using the New England Biolabs online software NEBaseChanger (primer sequences are available at doi: XXXX. The Q5 Site-Directed Mutagenesis Kit (New England BioLabs) was then used with the A/Baltimore/R0675/2019 hemagglutinin plasmid to produce plasmids with the desired mutations ...
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bioRxiv - Molecular Biology 2024Quote: ... where biotin-labeled primer (Bio-R1) was supplemented together with 2x NEB Next Ultra II Q5 Master Mix (M0544L, NEB). LAM products were purified with Streptavidin C1 dynabeads (65001 ...
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bioRxiv - Cell Biology 2024Quote: ... zact sequence was amplified with primers containing attB sites (F: GGGGACAAGTTTGTACAAAAAAGCAGGCTC-CATGGATGAGGAAATCGCTG; R: GGGGACCACTTTGTACA-AGAAAGCTGGGTAGAAGCACTTCCTGTGGACGATG) using a high-fidelity polymerase (Phusion, NEB). To create a middle entry clone pME-zact ...
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bioRxiv - Cell Biology 2020Quote: ... 250 ng of plasmid DNA was used per PCR reaction and used in a volume of 50 µl using Next High-Fidelity 2x PCR Master Mix (NEB, M0541) (according to the manufacturer’s protocol) ...
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bioRxiv - Genomics 2020Quote: Primer-walk PCRs were performed with edited single-cell clones to identify aberrant PCR products with OneTaq 2x Master Mix (NEB M0486L) following manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... After that tagmended library (20 µL) was PCR amplified using 25 µL NEBNext High-Fidelity 2X PCR Master Mix (NEB, #M0541L), 2.5 µL of P5_BRB primer (5 µM ...
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bioRxiv - Synthetic Biology 2020Quote: ... Reporter plasmids were assembled by Gibson Assembly using promoter PCR product and pHCKan-yibDp-GFPuv PCR product following manufacturer’s protocol (New England Biolabs, Ipswhich, MA). All plasmid sequences were confirmed by DNA sequencing (Genewiz ...
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bioRxiv - Genetics 2021Quote: ... barcodes and barcoding adapters (PCR Barcoding Expansion 1-96, EXP-PBC096, Oxford Nanopore Technologies, UK) by PCR using Q5 polymerase mastermix (NEB, USA) for individual fish identification according to manufacturer’s protocol in a 25μl reaction (98°C for 3 min ...
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bioRxiv - Genomics 2021Quote: ... Purified DNA was subjected to an initial step of PCR amplification consisting of 5 cycles using NEBNext High-Fidelity 2X PCR Master Mix (NEB, M0541S) and standard barcoded primers of Nextera kit for each sample ...
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bioRxiv - Plant Biology 2021Quote: ... The pooled PCR reactions were purified with the Monarch® PCR & DNA Cleanup Kit (5 μg) (New England Biolabs® Inc.) and run on 6% acrylamide gel ...
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bioRxiv - Synthetic Biology 2022Quote: ... DNA fragments to be assembled were amplified by PCR using Phusion High-Fidelity PCR Master Mix with GC Buffer (NEB, M0532S), purified with gel electrophoresis and Zymo clean Gel DNA Recovery Kit (Zymo Research,D4002) ...
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bioRxiv - Developmental Biology 2022Quote: ... TE buffer (20 µL) was added and PCR was performed using NEBNext High-Fidelity 2X PCR Master mix (New England Biolabs, M0541) as follows ...
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bioRxiv - Genetics 2020Quote: ... The remaining DNA was used to amplify fragments of the target site by PCR and amplicons were purified using the Monarch® PCR & DNA Cleanup Kit (NEB) following the manufacturer’s instructions and either sent for 250 bp paired end Illumina amplicon sequencing (Genewiz) ...
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bioRxiv - Molecular Biology 2020Quote: ... The PCR product was denatured and annealed in an 18 μl reaction (15 μl PCR product, 2 μl NEB Buffer2 (10X), 1 μl nuclease-free H2O ...
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bioRxiv - Genetics 2021Quote: ... 200-800 nt amplicons were amplified from genomic DNA from individual insertion lines through single fly PCR (Gloor et al., 1993) using OneTaq PCR master mix (NEB #M0271L). PCR conditions were 95°C for 30 seconds ...
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bioRxiv - Developmental Biology 2022Quote: ... Adaptor-ligated DNA fragments of proper size were enriched with PCR reaction using Fusion High-Fidelity PCR Master Mix kit (NEB, M0531S) and specific index primers supplied in NEBNext Multiplex Oligo Kit for Illumina (Index Primer Set 1 ...
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bioRxiv - Neuroscience 2022Quote: ... Three to four independently generated PCR products for each OT1-4/founder were purified using the Monarch PCR & DNA Cleanup Kit (NEB Inc.) and sent for Sanger sequencing at the OHSU Vollum Sequence Core.
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bioRxiv - Synthetic Biology 2022Quote: ... DNA fragments to be assembled were amplified by PCR using Phusion High-Fidelity PCR Master Mix with GC Buffer (NEB, M0532S), purified with gel electrophoresis and Zymoclean Gel DNA Recovery Kit (Zymo Research ...
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bioRxiv - Molecular Biology 2022Quote: ... Two to three PCR reactions per sample were combined and cleaned-up using the Monarch PCR and DNA clean-up kit (NEB, T1030S). 10-100 ng of DNA from each sample was carried forward for end-repair using the NEBNext Ultra II End repair/dA-tailing Module (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... Truncated EWSR1 constructs were created by PCR-based cloning from the His-MBP-EWSR1 expression vector using inverse PCR with Phusion DNA polymerase (New England Biolabs, F530S) then DpnI digest (New England Biolabs ...
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bioRxiv - Evolutionary Biology 2024Quote: ... A clean-up step was performed using Qiagen MinElute PCR purification kit and PCR-amplified using NEBNext Ultra Q5 DNA polymerase master mix (New England Biolabs®) with forward primer (5’-TAGAGCATGCACC GGCAAGCAGAAGACGGCATACGAGAT[N10]ATGTCTCGTGGGCTCGGAGATGT-3’ ...
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bioRxiv - Synthetic Biology 2023Quote: ... After confirming the PCR product size with gel electrophoresis the PCR product was incubated with DpnI (NEB, Ipswich, MA, Cat #R0176S) for 1 hour at 37°C to digest the plasmid template ...
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bioRxiv - Synthetic Biology 2023Quote: ... Linear plasmid fragments were generated by PCR and purified by Monarch® PCR and DNA cleanup kit (New England BioLabs®). Ligations were performed with In-Fusion® enzyme at 50°C for 15 minutes (min ...
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bioRxiv - Systems Biology 2022Quote: ... PCRs for screens with the vTR and CoV libraries were performed using NEBNext High-Fidelity 2X PCR Master Mix (NEB #M0541L) with 33 cycles ...
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bioRxiv - Genetics 2023Quote: ... were genotyped by amplifying the fourth coding region of EDNRB1 via PCR and digesting the PCR products with the restriction enzyme BsrI (New England BioLabs, #R0527S). Both deletions eliminate cut sites for BsrI and can thus be genotyped simultaneously (S1 Fig) ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCRs were conducted in volumes of 30 μL comprising a Phusion® High-Fidelity PCR Master Mix (New England Biolabs) (15 μL) ...
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bioRxiv - Developmental Biology 2023Quote: ... Eluted fragments were amplified by PCR with an appropriate number of PCR-cycles (5-8) using Custom NExtera PCR primers50 and the NEBNext High-Fidelity 2x PCR Master Mix (New England Biolabs, M0541S). Amplified DNA was purified using Qiagen MinElute Kit (Qiagen ...
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bioRxiv - Genetics 2023Quote: ... was genotyped by amplifying the fifth coding region of EDNRB1 via PCR and digesting the PCR products with the restriction enzyme SmaI (New England BioLabs, #R0141S). The R315P variant creates a cut site for SmaI (S2 Fig) ...
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bioRxiv - Cancer Biology 2023Quote: ... Transposed DNA was then purified on Diapure columns (Diagenode, Transposed purified DNA was then pre-amplified for 5 PCR Cycles using NEBNext High-Fidelity PCR MasterMix (NEB, M0541) and Illumina indexing primers ...
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bioRxiv - Synthetic Biology 2024Quote: ... DNA fragments to be assembled were amplified by PCR using Phusion High-Fidelity PCR Master Mix with GC Buffer (NEB, M0532S). The PCR mix underwent gel electrophoresis for purification ...
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bioRxiv - Genomics 2024Quote: ... The reaction was purified using the Zymo DNA Clean & Concentrator kit and PCR-amplified with NEBNext High-Fidelity 2× PCR Master Mix (NEB, M0541L) and primers as defined in Corces et al ...
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bioRxiv - Cancer Biology 2024Quote: ... Real-time quantititative PCR (qRT-PCR) detection was performed using the Luna® Universal qPCR Master Mix (M3003, New England Biolabs) and the CFX96 Real-Time PCR System ...
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bioRxiv - Cancer Biology 2024Quote: ... Ligation of sequences was performed by PCR-based cloning via addition of specific restriction sites using the Phusion High-Fidelity PCR Master Mix (New England Biolabs, #F531L), restriction digest and subsequent ligation using T4 DNA ligase (New England Biolabs ...
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bioRxiv - Cell Biology 2024Quote: ... Adaptor-ligated DNA fragments of proper size were enriched with PCR reaction using Phusion High-Fidelity PCR Master Mix kit (NEB, M0531S) and specific index primers supplied in NEBNext Multiplex Oligo Kit for Illumina (Index Primer Set 1 ...
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bioRxiv - Genomics 2024Quote: ... 10 µL of tagmented DNA was PCR amplified for 14 cycles in a 50 µL reaction volume using NEBNext High-Fidelity 2X PCR Master Mix (NEB # M0541S) and Nextera primers ...
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bioRxiv - Synthetic Biology 2024Quote: Transcription templates for expressing gRNA variants and SARS-CoV-2 or CMV input RNA fragments were generated by PCR (Phusion high-fidelity PCR kit, NEB #E0553) of the gBlock or Ultramer template that included a T7 promoter and the gRNA or input RNA coding sequence ...
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bioRxiv - Molecular Biology 2024Quote: ... Genomic regions flanking the expected cut sites for the indicated sgRNAs were PCR amplified using Q5 PCR Master Mix (NEB, #M0541L). PCR products were purified using the Monarch PCR and DNA Cleanup Kit (NEB ...
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bioRxiv - Developmental Biology 2024Quote: ... Amplification of cDNAs was performed using a high-fidelity KOD-Plus-Neo DNA polymerase (Toyobo, Japan) and resulting PCR products were cloned using NEB® PCR Cloning kit (New England BioLabs). Positive clones and plasmids were verified by DNA sequencing.
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bioRxiv - Molecular Biology 2024Quote: ... PCR and digest products were purified using Monarch DNA Gel Extraction and PCR and DNA Clean Up Kits (NEB T1020S, T1030S).