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Citations for New England Biolabs :
2201 - 2250 of 5089 citations for 8 4 Chlorophenylthio 2' O methyladenosine 3' 5' cyclic monophosphate sodium salt since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... The 5′ end was phosphorylated using T4 polynucleotide kinase (NEB, M0201L). A second bead binding was performed ...
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bioRxiv - Genomics 2022Quote: ... 5′ end phosphorylation using T4 polynucleotide kinase (NEB, Ipswich, MA; M0201), (iv ...
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bioRxiv - Microbiology 2022Quote: ... and subsequent restriction enzyme assay of exon 5 (with BSaaI, NEB) and exon 7 (BSErI ...
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bioRxiv - Microbiology 2022Quote: ... coli host strain NEB 5-alpha F’ lq (New England Biolabs) was exclusively used to generate ...
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bioRxiv - Immunology 2022Quote: ... 5 mol% labelled with SNAP-Surface Alexa Fluor 488 (NEB, S9128S), to 25% v/v HeLa lysate in a final volume of 20 µl (50 mM Tris-HCl ...
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bioRxiv - Systems Biology 2023Quote: ... alongside 5 U of quick alkaline phosphatase (calf intestinal, NEB #M0525S), all in a 30 µl rCutSmart buffer (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... 40 μl of 5 pM λ-phage DNA (New England Biolabs), digoxigenin-labelled at both ends14 ...
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bioRxiv - Molecular Biology 2024Quote: ... Purified RNA was treated with RNA 5′ pyrophosphohydrolase (New England Biolabs) for 2 h at 37℃ ...
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bioRxiv - Genomics 2023Quote: ... RNA was digested with addition of 5 U RNaseH (NEB, M0297) to the first strand cDNA synthesis reaction and incubation at 37 °C for 20 min.
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bioRxiv - Genomics 2023Quote: ... RNA was digested with addition of 5 U RNaseH (NEB, M0297) to the first strand cDNA synthesis reaction and incubation at 37°C for 20 min ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 5 µL of 10x T4 DNA Ligase Buffer (NEB #B0202). The reaction samples were incubated at room temperature for two hours and purified by FastGene PCR/Gel Extraction Kit (Nippon Genetics #FG-91302) ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was transformed into NEB 5-alpha cells (New England Biolabs) and plated on LB plates with carbenicillin for selection purposes and incubated overnight at 37 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by 5 µL of PNGase F (New England Biolabs, P0704L). The sample was incubated at 37°C for 3h to allow the de-N-glycosylation to proceed ...
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bioRxiv - Molecular Biology 2023Quote: ... ligated to the 5′ adapter with T4 RNA ligase (NEB M0204L), and reverse transcribed with Superscript III (Thermo Fisher 18080-051) ...
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bioRxiv - Microbiology 2023Quote: ... with the inclusion of m7G(5’)G RNA Cap Analog (NEB). Electroporation of the resulting RNA was carried out using a Neon® Transfection System (Invitrogen™ ...
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bioRxiv - Biochemistry 2023Quote: ... The purified DNA was 5’ phosphorylated with T4 Polynucleotide Kinase (NEB) and ligated with homemade T4 DNA ligase ...
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bioRxiv - Systems Biology 2023Quote: ... 5 μl of NEB T4 ligase (New England Biolabs, Ipswitch, MA) filled up to a volume of 100 μl with MilliQ H2O (Merck Millipore ...
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bioRxiv - Systems Biology 2023Quote: ... and 5 μL of 400 U/μl T4 DNA ligase (NEB). The ligation reaction was incubated for 4 h at 16°C with occasional inversion of the tube (every 1 h) ...
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bioRxiv - Molecular Biology 2023Quote: ... and then transformed into 5-alpha competent Escherichia coli cells (NEB), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... 5 μL of 400 U/μL T4 DNA Ligase (NEB #M0202), and incubating at room temperature for ≥4 hours with rotation.
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bioRxiv - Bioengineering 2023Quote: Right side probes were 5’ phosphorated with T4 Polynucleotide Kinase (NEB). Probes were then column cleaned with ssDNA/RNA Clean & Concentrator (Zymo D7010 ...
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bioRxiv - Cell Biology 2023Quote: ... Klenow fragment (5 U) & T4 polynucleotide kinase (15 U) (NEB; M0201). The A-tailing was performed with Klenow exo-fragment (15 U ...
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bioRxiv - Microbiology 2023Quote: ... and 5 μM dNTP in 17 μL 1x rCutSmart buffer (NEB). The RNA and primers were then annealed by incubating as follows ...
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bioRxiv - Neuroscience 2023Quote: ... 5 µL of Colour Prestained Protein Standard (New England Biolabs #P7719S) was included on each gel ...
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bioRxiv - Microbiology 2024Quote: ... Ligation of 5’ linker using T4 RNA ligase 1 (NEB; #M0204S) was conducted after phosphorylating the 5’ end of the pooled fragments with T4 PNK (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... 72 °C for 1 min) × 5] using NEBNext 2xMasterMix (NEB, M0541S) with Ad1_noMX and v2_Ad2.* indexing primers followed by qPCR amplification to determine additional cycle numbers ...
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bioRxiv - Microbiology 2020Quote: ... amplified with the primers Dhm1275-1 and −2 (Table 2) using a NEBuilder HiFi DNA Assembly Kit (New England BioLabs, Ipswich, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... 5 μl of 100 ng/μl cDNA diluted from the previous step was combined with 2 μl block mix and 2 μl nuclease free water (NEB, catalog no. AM9937), then the cDNA-block oligo mix was incubated on a thermocycler under the following condition to allow block oligo mix to bind to 5’ and 3’ end of the cDNA molecule ...
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bioRxiv - Evolutionary Biology 2024Quote: ... was prepared by combining 2 µL of 100 µM guide RNA (gRNA) with 2 µL of 20 µM EnGen SpyCas9 NLS (NEB, Cat. No. M0646T), and incubated at room temperature for 2-4 hours ...
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bioRxiv - Developmental Biology 2021Quote: ... Top and bottom guide RNAs were annealed with T4 Polynucleotide Kinase (3’ phosphatase minus, NEB) and T4 DNA Ligase Buffer (NEB ...
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bioRxiv - Genomics 2022Quote: ... Genomic DNA was dephosphorylated by adding 3 μl of Quick CIP enzyme (NEB, Cat #M0508) to 5 μg of input DNA (in total volume of 24 μl at >210 ng/μl ...
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bioRxiv - Microbiology 2019Quote: ... and the product library was cleaned 3 times with XP AMPure Beads (New England BioLabs) to remove short fragments and primers (bead ratios 1X and 0.6X ...
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bioRxiv - Cell Biology 2019Quote: ... The bead-bound protein was incubated with 3 µM SNAP-Surface Alexa Fluor 647 (NEB) for 40-60 min at 4°C (to fluorescently label the protein) ...
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bioRxiv - Genomics 2021Quote: ... rRNA was removed from 3 replicates with NEBNext Bacteria rRNA Depletion Kit (New England Biolabs), while the other 3 were treated with NEBNext Depletion Core Reagent Set using custom probes targeted to Haloferax volcanii rRNA (Martinez-Pastor and Sakrikar ...
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bioRxiv - Neuroscience 2020Quote: ... These 3 DNA fragments were assembled into a vector backbone (NEB pMiniT vector, EcoRI linearized) with the NEBuilder HiFi DNA Assembly Cloning Kit (NEB Cat# E5520) ...
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bioRxiv - Biochemistry 2020Quote: ... was replaced with sequence for a 3×FLAG tag by Gibson Assembly (New England Biolabs) to create payload plasmid pSAB41 ...
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bioRxiv - Microbiology 2021Quote: ... coli MG1655 genomic DNA into pKK223-3 by NEBuilder HiFi DNA Assembly Cloning Kit (NEB) and the first 300 bp portion of leuA was translationally fused with the nano-Luc (nLuc ...
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bioRxiv - Genomics 2022Quote: ... dA was added to the 3’-end of DNA using Klenow fragment exo- (NEB: M0212S) and then the adapter was ligated to the end of DNA ...
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bioRxiv - Cancer Biology 2022Quote: ... 3’ ligation (on-bead) with a barcoded RNA adapter followed using T4 RNA Ligase (NEB). Samples were again stringently washed ...
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bioRxiv - Developmental Biology 2022Quote: ... and the 3’ homology arm were produced by PCR (Phusion DNA polymerase, New England Biolabs). The 5’ and 3’ homology arm DNA fragments were amplified from genomic DNA prepared from vasa-Cas9 flies ...
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bioRxiv - Cell Biology 2020Quote: ... and 3 µg/ml leupeptin were incubate with 17.5 U alkaline phosphatase (New England Biolabs) at 37°C for 1 h ...
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bioRxiv - Genomics 2021Quote: ... 1 μl of RNAse inhibitor and 3 μl of Antarctic phosphatase (New England BioLabs Inc.). We then performed a kinase treatment adding 4 μl of T4 Polynucleotide Kinase (PNK ...
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bioRxiv - Genomics 2021Quote: ... to 3’-P presenting in the RNA that was dephosphorylated using T4 PNK (NEB, #M0201S). Linker and RNA ligation was performed by ligating pre-adenylated linker to the 3’-OH of RNA using RNA ligase2 ...
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bioRxiv - Genomics 2021Quote: ... The 3′ adapter (sequence: AGATCG-GAAGAGCACACGTCTGAACTC) was ligated using T4 RNA Ligase 1 (NEB, M0204L) and purified nascent RNA using streptavidin beads (NEB ...
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bioRxiv - Microbiology 2024Quote: ... the pUC19-PRha-YFP-3×FLAG plasmid was inverse amplified by PCR (Q5 polymerase, NEB), omitting the yfp gene ...
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bioRxiv - Microbiology 2023Quote: ... The 3 fragments were ligated using the Gibson Assembly® Cloning Kit (New England Biolabs). The created pEXG2::ΔfahA and pEXG2::ΔpanC were transformed into E ...
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bioRxiv - Molecular Biology 2024Quote: ... Purified fragments were dephosphorylated at their 3′ ends with T4 polynucleotide kinase (New England Biolabs) at 37 °C for 1 h and ligated to a 3′ adaptor sequence using T4 RNA ligase 2 (truncated K227Q ...
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bioRxiv - Molecular Biology 2023Quote: ... The 3×HA-DHFR cassette was amplified from the resulting vector by Q5 polymerase (NEB) using primers containing a 50 nt overlap homologous to the either upstream or downstream regions of the TGRH88_003980_t1 stop codon (primers “flank fwd Tgurpl11m tagging” and “flank rev Tgurpl11m tagging” in table S9 ...
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bioRxiv - Cancer Biology 2023Quote: Equal number (3×106) of cells were harvested in ice-cold nuclear extraction buffer (NEB) (400 μl ...
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bioRxiv - Microbiology 2023Quote: ... The vigR 3’ UTR sequence was amplified from JKD6009 using Phusion Hot Start Polymerase (NEB) with primers incorporating the MS2 aptamer sequence (fused to 5’ end of vigR 3’ UTR ...