Labshake search
Citations for New England Biolabs :
2001 - 2050 of 5089 citations for 8 4 Chlorophenylthio 2' O methyladenosine 3' 5' cyclic monophosphate sodium salt since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... Assembled plasmids were transformed into NEB 5-alpha F’Iq (NEB) and standard chemically competent Escherichia coli transformation protocols were used to construct plasmid host strains ...
-
bioRxiv - Genomics 2023Quote: ... 5 μL of rSAP mix (rSAP (1 U, NEB, M0371L) in 1X CutSmart buffer (for MseI/NdeI digestions ...
-
bioRxiv - Cell Biology 2023Quote: ... NEB 5-alpha competent E.coli (NEB #C2987H, Ipswich, MA, USA) were transformed with ligated plasmid by heat shock at 42°C for 30 seconds ...
-
bioRxiv - Cancer Biology 2023Quote: ... Linker reactions were removed with 5′ deadenylase (New England Biolabs) and Rec J Exonuclease (NEB) ...
-
bioRxiv - Genomics 2023Quote: ... The library was transformed into 5-alpha electrocompetent cells (NEB), grown in liquid culture ...
-
bioRxiv - Genomics 2023Quote: ... The libraries were transformed into 5-alpha electrocompetent cells (NEB). The plasmid library was prepared by maxi-prep (Sigma) ...
-
bioRxiv - Biochemistry 2023Quote: ... and 5 µl of prestained protein ladder (New England Biolabs). The gel was electrophoresed at 150 V ...
-
bioRxiv - Cancer Biology 2023Quote: ... 5 μL of T4 PNK buffer (NEB, cat. no. M0201S) and 5 μL of 10 mM ATP (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2023Quote: ... and 5′-hydroxyl group repair (T4 polynucleotide kinase (M0201L; NEB) were conducted on beads ...
-
bioRxiv - Developmental Biology 2023Quote: ... Protruding 5’ ends were filled in with Klenow enzyme (NEB) in the presence of [P]-32 dCTP (Hartmann Analytics ...
-
bioRxiv - Plant Biology 2024Quote: ... 5 and 7dpi using a total RNA extraction kit (NEB) and following the kit instructions ...
-
bioRxiv - Genomics 2024Quote: ... and dTTP with 5 U/µL DNA polymerase (NEB # M0210), the DNA was ligated by T4 DNA ligase (NEB # M0202 ...
-
bioRxiv - Systems Biology 2024Quote: ... 1 μL of Klenow DNA Polymerase (5 U/μL, NEB) and 2 μL of T4 PNK (10 U/μL ...
-
bioRxiv - Genomics 2022Quote: ... 2 μl of 10X CutSmart® buffer (1X final) and 2 μl of 1U/μl USER® II enzyme (M5505S, NEB) (0.1U/μl final ...
-
bioRxiv - Microbiology 2020Quote: ... stained with a mAb cocktail composed of SARS-CoV-2 spike (Creative-Bios; 2BCE5) and SARS-CoV-2 nucleoprotein (Creative-Biolabs; NP1C7C7) followed by anti-Mouse IgG-HRP (Abcam ab6823 ...
-
bioRxiv - Genomics 2021Quote: ... and supernatant was transferred to a new tube and added to 2 μL 10% SDS and 2 μL 20 mg/mL proteinase K (New England Biolabs P8107). Samples were incubated overnight at 65°C to reverse crosslinks ...
-
bioRxiv - Genetics 2023Quote: ... 5 µl of 100 ng/µLcDNA diluted from the previous step was combined with 2 µL of block mix and 2 µL of nuclease free water (NEB, AM9937), and then the cDNA block oligo mix was incubated on a thermocycler under the following conditions to allow block oligo mix to bind to the 5′ end and the 3′ end of the cDNA molecule ...
-
bioRxiv - Microbiology 2023Quote: ... antibiotic resistance cassette and 2 flanking regions were combined in equimolar amounts and mixed with 2 x HiFi reagent (NEB, UK) and incubated at 50°C for 1 hour ...
-
bioRxiv - Plant Biology 2021Quote: ... The final library products were further purified using an 8% polyacrylamide gel to excise 130-160nt products relative to the pBR322 DNA-MspI Digest ladder (New England Biolabs, Cat# E7323AA). The purified libraries were pooled and sequenced (single end 50 bp ...
-
bioRxiv - Microbiology 2019Quote: ... 10 (GSF1697) or 12 (GSF2248) PCR cycles were run using 8-nt barcoded oligos from NEBNext Multiplex Oligos barcode kit (NEB cat# 6609S). The libraries were purified with Agencourt AMPure XP beads ...
-
bioRxiv - Genomics 2022Quote: ... samples were gently removed from the chamber and digested overnight at 37°C in 8 U mL-1 proteinase K (NEB, cat# P8107) in digestion buffer (1× TAE buffer ...
-
bioRxiv - Molecular Biology 2023Quote: ... was immersed in a 10-fold volume of digestion solution with 8 U/mL Proteinase K (P8107S, New England Biolabs, Ipswich, USA) in digestion buffer containing 50 mM Tris pH 8.0 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Libraries were amplified for 9 cycles for the hepatocyte libraries and 8 cycles for the liver organoid libraries using Phusion® High-Fidelity PCR Master Mix (NEB, M0531S) with the Nextflex primers ...
-
bioRxiv - Cell Biology 2020Quote: ... 3 µg of purified DNA was digested with 10 units I-SceI (NEB, R0694), 10 units I-CeuI (NEB ...
-
bioRxiv - Genomics 2020Quote: ... and followed by incubation with 3 μl (1 U/μl) of USER enzyme (NEB) at 37°C for 20 min ...
-
bioRxiv - Molecular Biology 2021Quote: ... 3 μg of RNA was resuspended in 6x Loading dye (Biolabs, cat. No B7024S) and separated on a 20% PA gel without urea run at 160 volts at 4°C ...
-
bioRxiv - Molecular Biology 2019Quote: ... Precipitated RNA 3’ends were first dephosphorylated by shrimp alkaline phosphatase (SAP) (NEB M0371), and 3’ RNA linker (20 µM ...
-
bioRxiv - Cancer Biology 2019Quote: ... KRAS and BRAF 3’ homology arms using high-fidelity Phusion Polymerase (New England BioLabs). The 5’ homology arm of KRAS spans the region Chr12:25245385-25245994 ...
-
bioRxiv - Plant Biology 2020Quote: ... adaptor-ligated fragments were treated with 3 µl of USER® Enzyme (NEB, USA) before selective PCR enrichment using Phusion® High-Fidelity DNA polymerase using Universal and index primers ...
-
bioRxiv - Plant Biology 2021Quote: ... and ligated at a 3:1 amplicon:plasmid ratio with t4 DNA ligase (NEB, US) overnight at 16°C ...
-
bioRxiv - Genetics 2022Quote: ... and ligating into the BbsI ligated U6:3 vector (T4 ligase NEB, BbsI NEB) (S3 Table) ...
-
bioRxiv - Genetics 2022Quote: ... and ligating into the BbsI ligated U6:3 vector (T4 ligase NEB, BbsI NEB) (S3 Table) ...
-
bioRxiv - Microbiology 2022Quote: ... 15 μl 20% SDS and 3 μl 20 mg/ml Proteinase K (NEB #P8107) was added to the sample and the mixture was incubated at 37 °C for 30 min with agitation at 1400 rpm to completely lyze the cells ...
-
bioRxiv - Immunology 2020Quote: ... and 3’ end A-overhangs were added by NEBNext® dA-Tailing Module (NEB). The A-tailed products were subsequently cloned into pGEM®-T Easy vector (Promega ...
-
bioRxiv - Genetics 2020Quote: ... remaining ssDNA oligonucleotides were digested by the addition of 3 μL exonuclease 1 (NEB) to 20 μL extracted DNA ...
-
bioRxiv - Synthetic Biology 2020Quote: ... This was done in 50 μl reactions using 3 μl dual index primers (NEB), 135 ng purified PCR product from the previous reaction and 25 μl Q5 high fidelity master mix (NEB) ...
-
bioRxiv - Genomics 2019Quote: ... in 1× Buffer 3 (Bionano Genomics) or 120 U of Nb.BssSI (New England Biolabs) in 1× NEBuffer 3.1 ...
-
bioRxiv - Physiology 2020Quote: ... 636 bp 3’ arm of homology) with the nicking enzyme Nb.Bsm1 (New England Biolabs) and purification from an alkaline-agarose gel ...
-
bioRxiv - Molecular Biology 2022Quote: ... and/or 10 U of T4 PNK (wild-type or 3’ phosphatase minus, NEB). For RtcB ligation ...
-
bioRxiv - Biochemistry 2022Quote: ... 3’A overhang were added to each fragment using the Klenow fragment (NEB #M0212M) and DNA was purified using MinElute PCR Purification Kit (QIAGEN #28006) ...
-
bioRxiv - Genomics 2022Quote: ... Illumina sequencing adapters containing uracil hairpin loop structure and 3′ T overhangs (NEB, #E7337A) were ligated to the DNA by adding 150 units of Quick T4 DNA Ligase (NEB ...
-
bioRxiv - Molecular Biology 2022Quote: ... 3’UTR mutant was obtained by using the Site Directed Mutagenesis (New England Biolabs). miR-19a ...
-
bioRxiv - Biochemistry 2024Quote: ... crosslinked RNA fragments were dephosphorylated at their 3’-ends using T4-PNK (NEB, M0201S) and ligated to a pre-adenylated 3’-adapter (L3-App ...
-
bioRxiv - Molecular Biology 2024Quote: ... HR template was divided into 3 parts and each part was amplified by NEB Q5 polymerase using AJ51 & AJ52 oligonucleotides for part 1 ...
-
bioRxiv - Molecular Biology 2023Quote: ... these products were ligated to a 3’ adapter (1× T4 RNA ligase buffer (NEB), 10% PEG-8000 ...
-
bioRxiv - Cell Biology 2023Quote: ... and Exon 3 amplified using Q5® High-Fidelity DNA Polymerase (New England Biolabs). The following primers were used ...
-
bioRxiv - Immunology 2022Quote: ... To digest remaining M1 tag forward oligo: 3 µl 20U/ µl Exonuclease I (NEB) was added and samples were incubated for 1 h at 37°C ExoI denaturation was performed for 5 min at 98°C ...
-
bioRxiv - Molecular Biology 2023Quote: ... free adapters were removed by adding 3 units of RecJf (New England Biolabs, M0264) and 1.7 units of 5ʹ deadenylase (NEB ...
-
bioRxiv - Microbiology 2023Quote: ... then barcoded RNA adapters were ligated to the 3′ end (T4 RNA Ligase, NEB). Samples were separated by SDS-PAGE and transferred to nitrocellulose membranes ...
-
bioRxiv - Plant Biology 2024Quote: ... was used for Level 1 and 3 assembly and SapI (New England BioLabs, USA) for Level 2 assembly ...