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Citations for New England Biolabs :
2151 - 2200 of 10000+ citations for Thyroxine T4 ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... were used with 1 µL T4 DNA Ligase Reaction Buffer (NEB, 10x). We found that using these enzymes resulted in higher efficiency and fidelity for the cloning of mismatched gRNA libraries ...
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bioRxiv - Cell Biology 2024Quote: ... The 3′ cDNA adapter was ligated by T4 RNA ligase 1 (NEB) by incubating at 25 °C for 16h ...
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bioRxiv - Plant Biology 2023Quote: ... Adapter Mix II (ONT, Oxford, UK) and Quick T4 DNA Ligase (NEB) were added and incubated for 10 minutes at room temperature and purified with 0.5X AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Microbiology 2023Quote: ... 140 U of T4 RNA ligase I enzyme (New England Biolabs, #M0437M). Samples were incubated O/N at 22°C with agitation ...
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bioRxiv - Bioengineering 2023Quote: ... catalog #70015-3) using T4 DNA ligase (New England Biolabs, catalog #M0202L). We then packaged ligation reactions using T7Select415-1b cloning kit (Novagen (EMD Millipore) ...
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bioRxiv - Biophysics 2023Quote: ... and simultaneous blunt ligation of the plasmid using T4 DNA ligase (NEB). For the N-terminal ybbR-mutant the tag was inserted between E15 and E16 in the β’-subunit ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Ligation reactions were performed using T4 DNA ligase (New England Biolabs, M0202S) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Cloning of the shRNA hairpin was performed using T4 ligase (NEB, M0202L) at a ratio of 3:1 ...
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bioRxiv - Microbiology 2023Quote: ... and ligation with T4 DNA ligase (New England Biolabs Cat. No. M0202), generating plasmids pSR38-pSR43 (see Figure S1 for plasmid map of pSR39 ...
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bioRxiv - Molecular Biology 2023Quote: ... beads were incubated with 0.5 U/ μl T4 RNA ligase 1 (NEB) in 1x ligase buffer (containing 1 mM ATP ...
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bioRxiv - Systems Biology 2023Quote: ... 10 μl of NEB T4 ligation buffer (New England Biolabs, Ipswitch, MA), 5 μl of NEB T4 ligase (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... and then subjected to end healing using T4 PNK (New England BioLabs). Resulting footprints were then used to generate sequencing libraries using the NEBNext Small RNA Library Prep Set for Illumina kit (New England BioLabs) ...
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bioRxiv - Developmental Biology 2024Quote: ... and FseI/AscI for CORIN and CTSK using T4 ligase (NEB, M0202).
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bioRxiv - Genomics 2024Quote: ... and the top 3 sgRNAs were individually cloned into single sgRNA expression vectors with direct capture cs1 sequences (Addgene # 122238)10 using restriction digest (BstXI and BlpI) and T4 ligation (NEB # M0202M). A non-targeting sgRNA sequence was included as well ...
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bioRxiv - Molecular Biology 2023Quote: ... Hybridized probes were ligated using T4 DNA ligase and SplintR ligase (NEB), and hybrids were purified using MyOne-C1 Streptavidin Dynabeads (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... The pre-mixed oligos were ligated with T4 DNA ligase (NEB, M0202) using the following reaction conditions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Ligation reactions were performed using T4 DNA ligase (New England Biolabs, M0202S) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... 2 μL of T4 RNA Ligase (10 000 U/mL, NEB, M0204S), and 10 μL of PEG 8000 50% (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... Beads were treated with 10 μl of T4 Polynucleotide kinase (PNK; NEB) in 100 μl of 1X T4 PNK Reaction buffer containing 1 U/μl SUPERase•In™ RNase Inhibitor at 37°C for 30 minutes ...
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bioRxiv - Genomics 2024Quote: ... and 400 U/µL of T4 DNA ligase (New England Biolabs M0202S). We observed that either 0.5:1 or 1:1 molar ratios of insert:backbone were optimal for library uniformity ...
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bioRxiv - Molecular Biology 2024Quote: ... The column-cleaned inserts are ligated using T4 DNA ligase (NEB M0202) to the linearized pAVA plasmid [DPN1 (NEB R0176 ...
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bioRxiv - Biochemistry 2024Quote: ... The ATP and ADP makers were created using T4 polynucleotide kinase (NEB). T4 PNK mixed with 128 µM ATP supplemented with 10 nM [α-32 P]-ATP ...
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bioRxiv - Genetics 2024Quote: ... We ligated the PCR fragments into the vectors using T4 ligase (NEB), transformed into E ...
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bioRxiv - Genomics 2024Quote: ... then ligated to reporter gene inserts in multiple reactions (NEB T4 ligase). Ligation products were transformed into electrocompetent cells and grown in liquid culture from which we prepared plasmid DNA.
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bioRxiv - Biochemistry 2024Quote: ... T4 ligase 0.5 µL (M0202L, New England Biolabs, Ipswich, Massachusetts, United States), BsaI-HF®v2 0.5 µL (R3733L ...
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bioRxiv - Biochemistry 2024Quote: ... T4 ligase 0.5 µL (M0202L, New England Biolabs, Ipswich, Massachusetts, United States), BsmBI-v2 0.5 µL (R0739L ...
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bioRxiv - Biochemistry 2024Quote: ... RNA was ligated using T4 RNA ligase 2 (M0242, New England Biolabs) to a preadenylated linker and purified with ROTI Aqua P/C/I for RNA extraction and precipitated with NaOAc ...
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bioRxiv - Genomics 2024Quote: ... Intramolecular circularization of the DNA was performed with T4 DNA ligase (NEB) and residual linear DNA was degraded by a cocktail of exonucleases containing Plasmid-Safe ATP-dependent DNase (Lucigen) ...
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bioRxiv - Molecular Biology 2024Quote: ... Chromatin was washed once by 1× T4 DNA ligase buffer (NEB, B0202) and pelleted by centrifugation.
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bioRxiv - Immunology 2019Quote: ... followed by site-directed mutagenesis using the Q-5 kit (New England Biolabs) in order to generate point mutations into MIC1 (MIC1-T126A/T220A ...
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bioRxiv - Molecular Biology 2020Quote: ... Adenylation of R1R was done using 5’ DNA Adenylation kit (New England Biolabs) according to manufacturer’s instructions and cleaned with Oligo Clean & Concentrator (Zymo Research ...
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bioRxiv - Genetics 2020Quote: ... and purified with Monarch PCR & DNA Cleanup Kit (5 μg) (NEB, Ipswich, USA), checked on an 1 % (w/v ...
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bioRxiv - Biophysics 2023Quote: ... DNA was purified using Monarch PCR & DNA Cleanup Kit (5 μg) (NEB, T1030S) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... The G4 sequence and larger 5′ UTR (three human β-globin 5′ UTR sequences in tandem) were inserted using the Q5 Site-Directed Mutagenesis Kit (NEB # E0552S). pcDNA3.1(+)/mEGFP was a kind gift from Jeremy Wilusz (Baylor College of Medicine) ...
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bioRxiv - Cancer Biology 2021Quote: ... Digested inserts and vectors were purified and ligated using T4 DNA Ligase (NEB). For bacterial transformation ...
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bioRxiv - Cell Biology 2020Quote: 3′ linker ligation (1x PNK buffer, 20 U T4 RNA ligase I (NEB), 20 U T4 RNA Ligase II truncated K227R ...
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bioRxiv - Genetics 2021Quote: ... and inserted into a digested pCFD3 backbone by T4 DNA ligase (NEB, M0202).
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bioRxiv - Molecular Biology 2021Quote: ... 0.4 μl of 10 U/μl T4 DNA ligase (4U final, NEB M0202L), 1X T4 DNA ligase reaction buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... The RNA linker L5 (Table S1) was ligated with T4 RNA ligase (NEB). RNA was purified by phenol/chloroform extraction ...
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bioRxiv - Synthetic Biology 2022Quote: ... 1 µl of T4 or T7 DNA Ligase (New England Biolabs, Ipswich, USA), 1 µl of BsaI-HFV2 (New England Biolabs) ...
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bioRxiv - Molecular Biology 2022Quote: ... Phusion polymerase (M0532) and T4 DNA ligase (M0202) were also obtained from NEB. Plasmid mini-prep kit (Cat ...
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bioRxiv - Molecular Biology 2020Quote: ... the fragmented RNA wasend-repaired with T4 PNK and Spermidine (New England Biolabs). The size selection step was omitted ...
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bioRxiv - Molecular Biology 2021Quote: ... and the viral genomes isolated and then re-circularized using T4 ligase (NEB) and transfected into early passage HFK from three donor backgrounds (Lifeline technology) ...
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bioRxiv - Molecular Biology 2021Quote: ... The samples were transferred to 7 ml of 1.15x T4 ligation buffer (NEB), incubated with 1% Triton X-100 for 1 hour at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA was phosphorylated for 1 h at 37oC with T4 Polynucleotide kinase (NEB or made in-house by the Molecular Biology Service ...
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bioRxiv - Molecular Biology 2021Quote: ... Ligation of overhangs was performed with 20,000 U of T4 DNA ligase (NEB) for 4 h at room temperature with rotation ...
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bioRxiv - Genomics 2020Quote: ... and ligation (NEB: NEBNext Quick Ligation Reaction Buffer, NEB Adaptor, Quick T4 Ligase). The libraries were size-selected with AMPure beads (Beckman Coulter) ...
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bioRxiv - Genomics 2020Quote: ... 4 μL of 3 U/μL NEB T4 DNA Polymerase (New England Biolabs), 1 μL of 5U/μL Klenow (New England Biolabs) ...
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bioRxiv - Genomics 2019Quote: ... and resuspended in 95 µL PNK solution (1× T4 DNA ligase buffer (NEB), 0.1% Triton X-100) ...
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bioRxiv - Molecular Biology 2021Quote: ... and miRNA-RNA chimeras were produced with T4 RNA Ligase I (NEB, M0204S). The beads were further washed and subjected to alkaline phosphatase treatment (Roche ...