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Citations for New England Biolabs :
2401 - 2450 of 10000+ citations for Thyroxine T4 ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... B and C oligonucleotides were phosphorylated with T4 polynucleotide kinase (NEB, Cat #M0201L) according to the manufacturer recommendations ...
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bioRxiv - Microbiology 2022Quote: ... Ligation between these digested products was performed using T4 DNA ligase (M0202S, NEB). 50μl of competent bacteria were next transformed with 5μl of the ligation reaction product ...
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bioRxiv - Neuroscience 2022Quote: ... into pUASTattB (Bischof et al., 2013) via BamHI digestion and T4 ligation (NEB). We linearized pQUASTattB or pUASTattB with EcoRI-XhoI to enable the insertion of transgenes via Gibson assembly (HiFi DNA Assembly ...
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bioRxiv - Microbiology 2022Quote: ... After the ligation of adaptors using T4 RNA ligase 1 (New England Biolabs), reverse transcription was executed ...
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bioRxiv - Microbiology 2022Quote: ... The insert was then ligated with T4 DNA ligase (New England Biolabs, UK). The TPA particles were produced ...
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bioRxiv - Microbiology 2022Quote: ... Linear products were phosphorylated with T4 Polynucleotide Kinase (New England Biolabs, MA, USA) for 1 hr at 37°C and circularized with T4 DNA Ligase (New England Biolabs ...
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bioRxiv - Plant Biology 2022Quote: ... the linear PCR product was phosphorylated using T4 polynucleotide kinase (New England Biolabs) and ligated with T4 DNA Ligase (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... the duplexes were ligated with the purified vector with T4 DNA ligase (NEB). The ligation products were transformed into DH5α bacteria for amplification ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was circularized using T4 RNA ligase 1 (Epicentre or New England BioLabs) and reverse transcribed using Superscript III (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... The annealed oligos were phosphorylated using T4 polynucleotide kinase (PNK, New England Biolabs). The phosphorylated and annealed oligos were then diluted in Tris-EDTA (TE ...
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bioRxiv - Neuroscience 2023Quote: ... and then linked together using T4 DNA ligase (New England Biolabs, Cat # M0202) to form postBirA* ...
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bioRxiv - Molecular Biology 2023Quote: ... in a single tube RNA fragments are sequentially dephosphorylated with T4 PNK (NEB), polyadenylated with E ...
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bioRxiv - Synthetic Biology 2022Quote: ... for assembly into levels 2 in 20 μl 1x T4 ligase buffer (NEB). The reaction condition was ...
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bioRxiv - Synthetic Biology 2022Quote: ... and 4.4 µl of T4 DNA Ligase at 400 U/µl (NEB, M0202) were added to the reaction and incubated for 30 minutes at 37°C followed by heat inactivation at 65°C for 10 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... and 10 units T4 Polynucleotide Kinase (PNK) enzyme (New England Biolabs, cat#M0201S) for 2 hours at 37°C in 1X T4 PNK buffer (New England Biolabs ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA-adaptors are ligased to eluted RNA using T4 RNA ligase (NEB, M0204L) at 23? for 1 hour and adaptor-ligated RNA was cleaned-up using Dynabeads MyOne Silane and eluted in 13.5ul of nuclease-free water ...
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bioRxiv - Plant Biology 2022Quote: ... sgRNA expression vectors and sgRNAs (inserts) were ligated by T4 DNA ligase (NEB) at room temperature for 1 h to generate three expression vectors ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 μl of BsaI-HFv2 and 1 μl of T4 DNA ligase (NEB).
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bioRxiv - Biochemistry 2024Quote: Ribosome-protected fragments were dephosphorylated using T4 Polynucleotide Kinase (M0201, New England Biolabs). Dephosphorylated RNA was extracted using ROTI Acqua P/C/I for RNA extraction (X985.1 ...
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bioRxiv - Genomics 2024Quote: ... and DNA end-repaired with 1X T4 DNA ligase buffer (New England Biolabs), 0.4 mM dNTP mix ...
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bioRxiv - Microbiology 2024Quote: ... the digested upstream and downstream fragments were ligated with T4 DNA ligase (NEB). The ligated fragments were inserted into the digested plasmid using ClonExpress MultiS One Step Cloning Kit (Vazyme) ...
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bioRxiv - Neuroscience 2024Quote: ... Single strand DNA oligonucleotides were ligated using T4 Polynucleuotide Kinase (New England Biolabs) using manufacturer instructions ...
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bioRxiv - Genomics 2024Quote: ... and treated with a mixture of USER enzyme and T4 polynucleotide kinase (NEB). DNA was circularized at a concentration of 5 ng/µl with T4 DNA ligase (NEB) ...
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bioRxiv - Genomics 2024Quote: ... Digested oligo pool and vector backbone were ligated using T4 DNA Ligase (NEB) at room temperature for 45 min and purified using SPRI ...
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bioRxiv - Microbiology 2024Quote: ... a ligation reaction was conducted using the T4 DNA ligase (New England Biolabs) with a vector-to-insert ratio of 1:3 ...
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bioRxiv - Genomics 2023Quote: ... were added to the reaction with T4 DNA Polymerase (1 unit; NEB, M0203) and incubated at 12°C for 15 min ...
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bioRxiv - Synthetic Biology 2022Quote: ... or digestion and ligation using T4-DNA ligase (NEB, Frankfurt am Main, Germany). Plasmid and primer sequences are listed in Tables S2 and S3 ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.75 µl 0.1 M DTT and 0.3 U/µl T4 Polynucleotide kinase (NEB), and then incubated at 37°C 30 min ...
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bioRxiv - Microbiology 2023Quote: ... and ligated into XbaI-digested pMH94 using T4 DNA ligase (New England Biolabs) at 16°C O/N ...
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bioRxiv - Microbiology 2023Quote: ... Ligation of the two digested fragments was achieved with T4 DNA ligase (NEB) followed by electroporation into E ...
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bioRxiv - Genetics 2023Quote: ... and treated with a mixture of USER enzyme and T4 polynucleotide kinase (NEB). DNA was circularized at a concentration of 5 ng/μL with T4 DNA ligase (NEB) ...
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bioRxiv - Microbiology 2023Quote: ... and 1 μl T4 RNA ligase 2 (truncated K227Q, NEB, 200,000 units/ml) and incubated at 16°C for 14 h ...
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bioRxiv - Bioengineering 2023Quote: ... Digested inserts and vectors were ligated with T4 ligase (New England Biolabs, M0202L) and transformed via heat shock into DH5α competent cells ...
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bioRxiv - Molecular Biology 2023Quote: Hi-C libraries were treated with 3 U of T4 DNA polymerase (NEB) in the presence of 0.1 mM dGTP (NEB ...
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bioRxiv - Genomics 2023Quote: ... and NEBNext Quick T4 DNA Ligase (New England Biolabs Inc., Ipswich, United States) were added to the DNA sample from the previous steps and incubated for 10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... and ligated into the appropriate vector with T4 DNA ligase (New England Biolabs). Recombinant plasmids were confirmed by PCR (GoTaq DNA Polymerase ...
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bioRxiv - Biochemistry 2023Quote: ... Oligo 1 and oligo 2 were annealed by T4 polynucleotide kinase (NEB, #M0201S) at 37 ℃ for 30 min and 95 ℃ for 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... Fragments and backbone were ligated using T4 DNA ligase (Cat# M0202) from NEB. E.coli strain BL21(DE3 ...
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bioRxiv - Biophysics 2023Quote: ... 2 μM X4L4 or 8 U/μL T4 DNA ligase (New England BioLabs) and an increasing amount of PEG 8k (w/v ...
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bioRxiv - Biochemistry 2023Quote: ... Digested lentiCRISPRv2 and annealed oligos were ligated with T4 DNA ligase (NEB, #M0202S) at 16 ℃ overnight and were heated to be inactivated at 65 ℃ for 10 min before chilling on ice ...
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bioRxiv - Immunology 2023Quote: ... RNA fragments were then 3lll-dephosphorylated using T4 polynucleotide kinase (New England Biolabs) for 6 hours at 37°C in 2-(N-morpholino)ethanesulfonic acid (MES ...
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bioRxiv - Immunology 2023Quote: ... The 3lll adaptor was added using T4 RNA ligase 1 (New England Biolabs) for 2.5 hours at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... and end labeling was performed using T4 PNK from NEB (cat number: M0201S) and biotin-14-dATP from Invitrogen (cat number ...
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bioRxiv - Cell Biology 2023Quote: ... The two purified fragments were then ligated using T4 DNA ligase (NEB, M0202) following manufacturer’s instructions and transformed into competent cells (NEB ...
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bioRxiv - Genetics 2023Quote: ... which were then ligated with eight barcoded adaptors using T4 DNA ligase (NEB). The constructs were then amplified by PCR using sets of primers with an additional 24 barcodes ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was phosphorylated for 1h at 37°C with T4 Polynucleotide kinase (NEB or made in-house by the Molecular Biology Service ...
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bioRxiv - Genetics 2023Quote: ... The ICU11_sgRNA1_F/R oligonucleotides were phosphorylated using T4 polynucleotide kinase (New England Biolabs) and hybridized in a thermal cycler (Bio-Rad Laboratories T100) ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids were constructed by standard restriction ligation methods using T4 DNA ligase (NEB) or using the NEBBuilder assembly kit (NEB) ...
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bioRxiv - Cancer Biology 2023Quote: ... Ligation was performed with 400 units of T4 DNA Ligase (New England Biolabs) at 16°C overnight ...
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bioRxiv - Biochemistry 2023Quote: ... The final Golden Gate reaction volume contained 1× concentrated T4 ligase buffer (NEB), 250 ng Golden Gate vector (pcDNA3-based carrying a twin Strep-FLAG tag ...