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Citations for New England Biolabs :
1951 - 2000 of 10000+ citations for Thyroxine T4 ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The PCR product was ligated using T4 ligase (New England Biolabs) and transformed into E ...
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bioRxiv - Genomics 2023Quote: ... washed with 500 μl 1x T4 DNA Ligase Buffer (NEB #B0202A) and collected by centrifugation (10000x g ...
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bioRxiv - Neuroscience 2023Quote: ... RNAs on the resin were treated with T4 PNK (NEB, M0201) supplemented with murine RNase inhibitor at 37 °C for 20 min and washed with Halo binding buffer with 1% Triton X-100 (three times) ...
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Transcriptional Isoforms of NAD+ Kinase regulate oxidative stress resistance and melanoma metastasisbioRxiv - Cancer Biology 2023Quote: ... Constructs were ligated (In-Fusion HD, Takara or T4 Ligase, NEB) into pLenti vector behind an EFS promoter using BamHI and NsiI sites ...
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bioRxiv - Microbiology 2024Quote: ... and T4 DNA ligase were purchased from New England Biolabs (NEB). PCR was performed with Taq (Qiagen ...
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bioRxiv - Biophysics 2024Quote: ... Inserts were phosphorylated by incubation with T4 polynucleotide kinase (NEB, #M0201) in T4 DNA ligase buffer (# ...
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bioRxiv - Systems Biology 2024Quote: ... Ligation reactions (10 µl) contained 1X T4 DNA ligase buffer (NEB), additional DTT (10 mM final ...
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bioRxiv - Plant Biology 2023Quote: ... 1D2 Adapters (ONT, Oxford, UK) and Quick T4 DNA Ligase (NEB) were added and incubated for 10 minutes at room temperature and purified with 0.4X AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Plant Biology 2024Quote: ... or ligated using T4 ligase (Thermo Fisher Scientific/New England Biolabs) in corresponding clones.
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bioRxiv - Molecular Biology 2023Quote: ... or blunt-end cloning with T4 PNK (New England Biolabs, USA) and T4 DNA ligase (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting PCR product was ligated (T4 Ligase, New England Biolabs) with AhdI-digested (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 unit/µl T4 RNA ligase 1 (New England Biolabs, M0204S) and incubated at 23°C for 150 min ...
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bioRxiv - Molecular Biology 2023Quote: ... and 20 units of T4 Polynucleotide kinase (M0201L, New England Biolabs), in a total volume of 50 uL ...
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bioRxiv - Molecular Biology 2023Quote: ... the ligation mix with 20 U T4 RNA Ligase buffer (NEB) was added and incubated for 3 hours at 25 °C ...
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bioRxiv - Synthetic Biology 2023Quote: ... in 1x T4 DNA ligase buffer with 10 µM ATP (NEB) in 10 µL reactions ...
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bioRxiv - Immunology 2023Quote: ... End dephosphorylation was performed using T4 polynucleotide kinase (PNK) (NEB M0201) for 1 hour at 37°C and ligation was performed using the T4 RNA ligase I (NEB M0204 ...
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bioRxiv - Biophysics 2024Quote: ... and the second fragments for ligation with T4 DNA ligase (NEB) following the manufacture manual ...
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bioRxiv - Developmental Biology 2024Quote: ... The dephosphorylation reaction was supplemented with 0.5 μl T4 PNK (NEB), and the reaction was incubated for 25 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... The resulting PCR product was treated with T4 PNK (NEB M0201S) and T4 DNA ligase (NEB M0202S) ...
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bioRxiv - Genomics 2023Quote: ... We annealed oligos in the presence of T4 Polynucleotide Kinase (NEB). We then performed Golden Gate assembly with diluted oligos ...
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bioRxiv - Immunology 2023Quote: ... 3 μL of T4 RNA ligase buffer (NEB, cat. no: M0204S), 1 μL of 10 mM ATP (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... The fragmented RNA was 3’-end repaired using T4 PNK (NEB) and ligated to RNA adapter (5’-/5rApp/AGATCGGAAGAGCGTCGTG/3SpC3/-3’ ...
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bioRxiv - Plant Biology 2023Quote: ... 1D2 Adapters (ONT, Oxford, UK) and Quick T4 DNA Ligase (NEB) were added and incubated for 10 minutes at room temperature and purified with 0.4X AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Molecular Biology 2024Quote: ... The following antisense oligonucleotides were 32P-labelled with T4 PNK (NEB) and used as probes ...
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bioRxiv - Biochemistry 2024Quote: ... Digested genomic DNA was ligated using T4 ligase (NEB, Ipswich, MA) at 16°C for 16 hr ...
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bioRxiv - Biochemistry 2024Quote: ... and 1 mM DNA oligo in T4 polynucleotide kinase buffer (NEB). This mixture was then incubated at 37°C for 1 hour ...
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bioRxiv - Bioengineering 2024Quote: ... DNA circularization was performed using T4 DNA ligase (NEB, Ipswich, MA), followed by treatment with a mixture of exonucleases to remove residual linear DNA ...
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bioRxiv - Biochemistry 2024Quote: ... The DNA Fragments were the ligated using T4 DNA ligase (NEB) and amplified using XL10-gold ultracompetent cells ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were treated with 3ul T4 PNK (New England Biolabs M0201), 1x PNK buffer ...
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bioRxiv - Genomics 2024Quote: ... and hydroxyl repair was performed using T4 Polynucleotide Kinase (PNK; NEB). Purified RNAs were subsequently ligated with the 5’ RNA adaptor (RA5 ...
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bioRxiv - Microbiology 2024Quote: ... Linker was ligated into purified linearized vector using T4 ligase (NEB) at 4°C overnight followed by transformation into DH5α E ...
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bioRxiv - Microbiology 2024Quote: ... The plasmids were constructed using T4 DNA ligase (New England Biolabs) with a ratio of 3:1 of insert to backbone ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA ends were then blunted using T4 DNA polymerase (NEB, M0203L) and Klenow (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by ligation with T4 DNA ligase (New England Biolabs, M0202) following the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2024Quote: ... followed by ligation with T4 DNA ligase (New England Biolabs, M0202) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... 1X T4 RNA ligase 1 buffer (New England Biolabs, cat#B0216L), 25% PEG8000 with 1μl (40U ...
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bioRxiv - Plant Biology 2023Quote: ... and 1μl T4 RNA ligase 1 (New England Biolabs, cat#M0204S) in a reaction volume of 100μl ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were analysed by an MSD Elisa assay (Pacific Biolabs) using a CEP55 antibody (Novux ...
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bioRxiv - Microbiology 2022Quote: ... The purified cDNA was then ligated with 5’ adapter (5’/5Phos/AGATCGGAAGAGCGTCGTGTAGCTCTTCCGATCTN10/3SpC3/-3’ with 1:20 molar ratio of RNA to 5’adapter) using Quick Ligation Kit (NEB) at 37°C overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... The 3’ adapter (RNA oligo: 5’P-GAUCGUCGGACUGUAGAACUCUGAAC-3’InvdT) was pre-adenylated prior to use (5’ DNA adenylation kit, NEB, according to the manufacturer’s instructions) ...
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bioRxiv - Neuroscience 2022Quote: ... and oligos annealed with adapters to the sense 5’ - CACC and antisense 5’-AAAC were ligated using Quick Ligase Kit (New England Biolabs). Ligated product was transformed into stabl3 competent E ...
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bioRxiv - Biochemistry 2022Quote: ... The 3′ adapter was conjugated with an amino CA linker instead of dCC at the 3′ end (GeneDesign) and adenylated using 5′ DNA adenylation kit at the 5′ end (NEB). To reduce a ligation bias ...
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bioRxiv - Synthetic Biology 2022Quote: ... NNN spacer to increase library diversity during sequencing (preadenylated oligos were prepared by 5’ DNA adenylation kit (E2610L) using thermostable 5’ App DNA/RNA ligase (M0319L, both from New England Biolabs) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... The 3′ adapter was conjugated with an amino CA linker instead of dCC at the 3′ end (GeneDesign) and adenylated using 5′ DNA adenylation kit at the 5′ end (NEB). To reduce a ligation bias ...
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bioRxiv - Molecular Biology 2024Quote: ... The CHD4-GFP mutant was cloned with selective primers (fwd, 5’-GGATGCTACAGGTGGAACCCTGCACCCCTA-3’; rev, 5’-GCCCAGGCCCGACGCCAT-3’) and a Q5 site-directed mutagenesis kit (NEB) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... siRNA resistant mCherry-ALG-2 was created by mutagenesis (primers sequences 5’-ATTTCGATGTTTGACCGTGAGAAC-3’, 5’-AATGGACCTGACAGTCACTGGATTAAA-3’) using a Q5 Site-Directed Mutagenesis Kit (E0554S, NEB). The plasmid encoding IST1-GFP is as described (63 ...
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bioRxiv - Molecular Biology 2020Quote: This oligonucleotide was adenylated using the 5’ DNA adenylation kit (NEB, E2610S) as follows ...
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bioRxiv - Molecular Biology 2020Quote: ... The linkers were pre-adenylated with a 5’ DNA Adenylation kit (NEB), and then used for the ligation reaction ...
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bioRxiv - Molecular Biology 2019Quote: ... The linkers were pre-adenylated with a 5’ DNA Adenylation kit (NEB), and then used for the ligation reaction ...
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bioRxiv - Molecular Biology 2020Quote: ... A mix of 5 μl 3’Ligation Reaction Buffer 2x (NEB-kit) and 1.5 μl of 3’Ligation Enzyme Mix (NEB-kit ...