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Citations for New England Biolabs :
2351 - 2400 of 10000+ citations for Thyroxine T4 ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: ... The resulting PCR products were self-assembled using T4 ligase (New England Biolabs) to obtain the desired SSGM plasmid libraries ...
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bioRxiv - Plant Biology 2021Quote: ... The digested plasmid and the hybridized oligonucleotides were ligated using T4 Ligase (NEB) and then transformed into Escherichia coli DH5α competent cells ...
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bioRxiv - Microbiology 2020Quote: ... The reaction was supplemented with 3 μl of T4 RNA ligase I (NEB), 1.5 μl of 10X reaction buffer ...
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bioRxiv - Biophysics 2021Quote: ... A dT50 oligo was radioactively labeled with 32P by T4 PNK (NEB M0201). EMSAs were performed in Imaging Buffer at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 μL 400 U μl-1 T4 DNA Ligase (NEB, high concentration formula) and 664 μL H2O and incubated for 120 mins at 23°C with 300 rpm slow rotation ...
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bioRxiv - Genomics 2022Quote: ... We ligated a 3’ adapter ligation using T4 RNA Ligase 1 (NEB, M0204L). We performed a second bead binding followed by a 5’ decapping with RppH (NEB ...
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bioRxiv - Genomics 2022Quote: ... The annealed oligo is ligated into the backbone using T4 DNA Ligase (NEB) in a typical 10μL ligation reaction with 1μL of T4 DNA ligase ...
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bioRxiv - Cancer Biology 2022Quote: Oligos of the gRNAs were annealed with T4 polynucleotide kinase (New England Biolabs) by PCR ...
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bioRxiv - Biochemistry 2022Quote: ... and resultant DNA was used for ligation reactions with T4 DNA Ligase (NEB). Ligated product was transformed directly into chemically competent NEB5α cells (NEB ...
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bioRxiv - Bioengineering 2022Quote: ... The purified fragments were ligated together using T4 DNA Ligase (NEB, Cat#M0202S) to create pNHT7 (Figure S6A).
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bioRxiv - Plant Biology 2021Quote: ... the DNA sequence was re-ligated using T4 DNA ligase (New England Biolabs) according to the manufacturer’s protocol in 20 μl reactions incubated overnight at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... circular RNAs were obtained by ligation using T4 RNA ligase (New England Biolabs), followed by phenol-chloroform extraction ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA was removed 3’phosphoryl groups using T4 Polynucleotide Kinase (New England Biolabs) in the buffer (70 mM Tris-HCl ...
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bioRxiv - Developmental Biology 2022Quote: ... a final concentration of 120ug BSA and 4,000U T4 DNA ligase (NEB, M0202) at 16C for 4 hours with gentle rocking ...
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bioRxiv - Microbiology 2022Quote: RNAs for direct cDNA sequencing were treated with T4 Polynucleotide Kinase (NEB, M0201) following the manufacturer’s non-radioactive phosphorylation protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 ul RNaseOUT and 1ul T4 RNA ligase 2 truncated K227Q (NEB M0351). Samples were then washed twice with PNK buffer ...
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bioRxiv - Developmental Biology 2019Quote: ... Oligo pairs were annealed and phosphorylated by T4 Polynucleotide kinase enzyme (NEB, M0201S), chloroform extracted ...
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bioRxiv - Microbiology 2019Quote: ... cholerae RNA at 37°C with 20 units of T4 RNA ligase (NEB) in a 20 µL reaction containing 1X T4 RNA ligase buffer ...
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bioRxiv - Cell Biology 2019Quote: ... 25 ng of the PCR product was ligated with T4 DNA Ligase (NEB) in a final volume of 10 μL for 1h at RT ...
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bioRxiv - Microbiology 2019Quote: ... Extracted crRNAs were phosphorylated with T4 Polynucleotide Kinase (New England Biolabs, MA, USA), radiolabeled with γ-[32P]-ATP (PerkinElmer ...
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bioRxiv - Microbiology 2019Quote: ... An aliquot of the purified amplicons was phosphorylated using T4 Polynucleotide Kinase (NEB) using the manufacturer’s recommended reaction mixture and we extended the incubation ...
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LIR-dependent LMX1A/LMX1B autophagy crosstalk shapes human midbrain dopaminergic neuronal resiliencebioRxiv - Cell Biology 2019Quote: ... Primers were annealed and ligated with the digested promoter with T4 ligase (Biolabs). Cells were seeded on CELLSTAR® 96-well white plate (Greiner Bio-one ...
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bioRxiv - Developmental Biology 2019Quote: ... Each oligo pair was annealed using T4 Polynucleotide Kinase (PNK) enzyme (NEB, M0201S) and then cloned into the sgRNA(MS2)_puro backbone (Addgene 73795 ...
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bioRxiv - Microbiology 2019Quote: ... and 1x T4 DNA ligase buffer with 10 mM ATP (NEB cat. # B0202). Reactions were then heat-killed at 75°C for 20 minutes and 2.5 µl of 1 µM pre-annealed ...
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bioRxiv - Cancer Biology 2019Quote: ... All three DNA pieces were ligated by incubating them with T4 ligase (NEB) overnight at 16°C.
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bioRxiv - Molecular Biology 2020Quote: ... phosphorylated with 10 U of phosphatase-free T4 polynucleotide kinase (New England BioLabs) for 30 min at 37°C ...
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bioRxiv - Immunology 2020Quote: ... 1 mM EDTA) and phosphorylated using T4 Polynucleotide Kinase (New England BioLabs M0201L) according to manufacturer recommendations ...
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bioRxiv - Molecular Biology 2021Quote: ... then ligated with the phosphorylated PCR fragments by T4 DNA Ligase (NEB, M0202S). Inserts were analyzed by Sanger sequencing ...
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bioRxiv - Molecular Biology 2020Quote: ... after which the CRISPR inserts were ligated in using T4 DNA ligase (NEB). CRISPR inserts were generated by annealing two complementary oligos containing a 4bp adapter serving as the BsmBI sticky end.
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bioRxiv - Molecular Biology 2020Quote: ... which contains Agrobacterium tumefaciens backbone vector using T4 DNA ligase (New England Biolabs). Resultant plasmid ...
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bioRxiv - Microbiology 2021Quote: ... The DNA fragments were ligated using a T4 DNA ligase (New England Biolabs). The point mutations in VP1881 to generate the VP1881AAA variant were introduced by overlapping PCR (SOEing-PCR) ...
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bioRxiv - Biochemistry 2021Quote: ... or by ligation using T4 DNA (New England Biolabs, Frankfurt am Main, Germany). Plasmid and primer sequences are given in Tables S1 and S2 ...
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bioRxiv - Synthetic Biology 2020Quote: ... Aliquots (50 µl) were washed again and resuspended in T4 ligase buffer (NEB) containing pre-annealed nicked DNA substrate (MR9/MR10-f1/MR11-54PS or as specified ...
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bioRxiv - Genetics 2020Quote: ... by digestion with SalI and NheI and ligation with T4 DNA ligase (NEB).
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bioRxiv - Cell Biology 2020Quote: ... and the fragment inserted matching with the BspQI sites using T4 ligase (NEB), according to the supplier’s instructions.
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bioRxiv - Genomics 2021Quote: ... the chromatin was ligated by adding 2000U of T4 DNA ligase (NEB, M0202M) and 10x T4 DNA ligase buffer to a total volume of 650μL and incubating the solution overnight at 16°C and 300 RPM ...
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bioRxiv - Molecular Biology 2020Quote: ... 6μl 20mg/ml BSA and 5μl 400U/μl T4 DNA Ligase (NEB M0202) for 3h on a rotor at RT ...
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bioRxiv - Developmental Biology 2021Quote: ... The purified RNA was dephosphorylated with 1 µL of T4 polynucleotide kinase (NEB) in 1x T4 PNK buffer for 1 h at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... by incubating the DNA with T4 polynucleotide kinase (10 units; New England Biolabs) in the reaction medium provided by the supplier for 30 min at 37 °C ...
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bioRxiv - Genomics 2021Quote: ... 20% PEG8000 and 20 U T4 RNA Ligase 1 (New England Biolabs, M0204) at 16°C for 12 hours.
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bioRxiv - Molecular Biology 2021Quote: ... and the viral genomes isolated and then re-circularized using T4 ligase (NEB) and transfected into early passage HFK from three donor backgrounds (Lifeline technology) ...
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bioRxiv - Microbiology 2020Quote: ... and ligation with T4 ligase (Bioconcept) or by Hi-Fi Gibson assembly (NEB). PCR were performed using Phusion polymerase (Life Technologies ...
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bioRxiv - Biochemistry 2020Quote: ... restriction enzymes and T4 DNA ligase were obtained from New England Biolabs (NEB). Sanger sequencing was performed by Genewiz Incorporated.
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bioRxiv - Microbiology 2020Quote: ... and an ONT adapter was ligated each end (Quick T4 Ligation Module, NEB). Following ...
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bioRxiv - Molecular Biology 2021Quote: ... The digested nuclei were ligated with 1 μl of T4 DNA ligase (NEB) in 100 μl of 1 × T4 ligation buffer for 24 h at 16°C ...
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bioRxiv - Molecular Biology 2020Quote: ... the plasmid was ligated by T4 ligase (New England Biolabs, cat# 101228-176) and transformed into Agilent XL1-Blue Electroporation-Competent Cells (Agilent ...
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Glucocorticoid receptor collaborates with pioneer factors and AP-1 to execute genome-wide regulationbioRxiv - Genomics 2021Quote: ... In-line barcoded 3′ adapters were ligated with T4 RNA ligase I (NEB) for 12 hours at 20°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The gene fragments were ligated into the backbone using T4 DNA ligase (NEB) and transformed into XL10-Gold Ultracompetent bacteria (Agilent) ...
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bioRxiv - Biochemistry 2022Quote: ... Modular cloning using the restriction enzyme BsaI and T4 ligase (New England Biolabs) was used to generate recombinant plasmids containing UHOR_02700 and all other proteins used in this study ...
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bioRxiv - Developmental Biology 2022Quote: ... 1 mM ATP with 60U of High Concentration T4 RNA ligase (NEB, M0437M) at 16°C overnight ...