Labshake search
Citations for New England Biolabs :
2101 - 2150 of 7434 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2021Quote: ... The PCR product was purified using Monarch PCR and DNA Cleanup kit (New England Biolabs, Inc., Ipswich, MA, USA) and DNA concentration was quantified using NanoDrop spectrophotometer (Thermo Fisher Scientific ...
-
bioRxiv - Genomics 2020Quote: ... Transposed DNA was PCR amplified using the NEB Next High-Fidelity PCR Master Mix (New England Biolabs, Hitchin, UK) and primer Ad1_noMX in combination with one of six barcoded primers (Table 1 ...
-
bioRxiv - Genomics 2020Quote: Quantification of several genes in sequence libraries was performed by real-time PCR using NEBnext PCR Master Mix (NEB) supplemented with SYBR Green (Applied Biosystems) ...
-
bioRxiv - Developmental Biology 2019Quote: ... DNA libraries were made by a limited cycle PCR Using the NEBNext High-fidelity 2X PCR Master Mix (NEB). To maximize the complexity of DNA libraries ...
-
bioRxiv - Microbiology 2019Quote: Index PCR was performed with 25µL NEBNext Hig-Fidelity 2X PCR master mix (New England Biolabs Inc., Ipswich, MA), containing 5µL of each index primer (Nextera XT Index kit v2 set A ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... We performed a 10-cycle PCR using a Phusion PCR Kit according to the manufacturer’s protocol (New England Biolabs) with multiplexed primers and adjusted PCR products to 10 μM for sequencing on either an Illumina HiSeq2500 (125 bp paired-end ...
-
bioRxiv - Developmental Biology 2021Quote: ... MgCl2 was added to a final concentration of 50mM and 12.2µl of each tagmented sample was PCR amplified for 15 cycles using 2x NEB Next HiFi PCR mix (NEB). Amplified libraries were purified using the Qiagen PCR purification MinElute kit.
-
bioRxiv - Developmental Biology 2021Quote: ... MgCl2 was added to a concentration of 50mM and 16µl of each sample were PCR amplified using 2x NEB Next HiFi PCR mix (NEB) and 16 PCR cycles.
-
bioRxiv - Cancer Biology 2020Quote: ... The promoter region and nanoluciferase coding region was PCR amplified from the pNL1.1 reporter constructs using Phusion High-Fidelity PCR Master Mix with GC Buffer (NEB), and 400 nM of forward and reverse primers (pNL1.1_forward 5’-AATTATCTTAAGATTTCTCTGGCCTAACTGGCCGG and pNL1.1_reverse 5’-AATTATCTTAAGTGGGTTGAAGGCTCTCAAGGGCATC) ...
-
bioRxiv - Microbiology 2020Quote: ... Quantitative real time PCR (qRT-PCR) was performed with the Luna Universal qPCR Master Mix (New England Biolabs #M3003) on a CFX Connect instrument (BioRad #1855200) ...
-
bioRxiv - Microbiology 2021Quote: ... Quantitative real time PCR (qRT-PCR) was performed with the Luna Universal qPCR Master Mix (New England Biolabs #M3003) on a CFX Connect instrument (BioRad #1855200) ...
-
bioRxiv - Microbiology 2020Quote: ... all PCR products were purified using the Monarch® PCR and DNA Clean-up Kit (New England Biolabs, USA) following the manufacturer’s instructions and eluted in molecular grade water.
-
bioRxiv - Zoology 2021Quote: ... The PCR mixtures for first step and nested PCR step (12.5 μl) contained 1X OneTaq Hot Start Master Mix (NEB) and 0.2 μM of each primer ...
-
bioRxiv - Genomics 2020Quote: ... a PCR reaction was run on the diluted sample using 25μL Q5 Hot Start PCR Master Mix (M0494S, NEB) and 2.5μL (20μM ...
-
bioRxiv - Genetics 2022Quote: ... All PCR reactions were conducted using Q5 High-Fidelity 2X PCR Master Mix following the manufacturer’s directions (New England Biolabs). These fragments were transformed into S ...
-
bioRxiv - Microbiology 2021Quote: ... The entire ORF of mucoricin was PCR amplified from cDNA using Phusion High-Fidelity PCR Kit (New England Biolabs) using the primers 5’-GATAAGACTAGTATGTATTTCGAAGAAGGC-3’ and 5’-GGTGATGCACGTGTCCTTCAAATGGCACTA-3’ ...
-
bioRxiv - Cancer Biology 2021Quote: PCR amplifications were carried out using Phusion® High-Fidelity PCR Master Mix with HF Buffer (NEB, Cat#M0531L) according to the manufacturer’s instructions (25 µl reaction) ...
-
bioRxiv - Microbiology 2021Quote: ... Bacterial 16S rRNA V4 fragments were PCR amplified using NEBNext Q5 Hot Start HiFi PCR master mix (NEB, U.K.) with universal 16S rRNA V4 primers [564F (TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG-AYTGGGYDTAAAGNG ...
-
bioRxiv - Genetics 2022Quote: ... PCR reactions were performed using NEBNext® High-Fidelity 2X PCR Master Mix (New England BioLabs, catalog number M0541) following the manufacture’s protocol unless specified differently ...
-
bioRxiv - Cancer Biology 2020Quote: ... sgRNAs were amplified using a one-step barcoding PCR using NEBNext High Fidelity 2X PCR Master Mix (NEB, M0541L) and the following primers:
-
bioRxiv - Cancer Biology 2021Quote: ... Libraries were generated by 7 rounds of PCR amplification using NEBNext High-Fidelity 2X PCR Master Mix (NEB, M0541L), purified using SPRIselect reagent kit (Beckman Coulter ...
-
bioRxiv - Immunology 2022Quote: ... DNA amplification via PCR was performed using a large-scale PCR system using Q5 polymerase (New England BioLabs, USA) and resulted in 2780 bp linDNA amplicon expression cassettes ...
-
bioRxiv - Molecular Biology 2022Quote: ... and a PCR with 10-13 cycles was performed using the NEBNext High Fidelity 2X PCR Master Mix (NEB) and Ad1_noMX and Ad2.1–2.12 barcoded primers described in (30) ...
-
bioRxiv - Bioengineering 2022Quote: ... the 5S and Saba PCR products were purified using Monarch® PCR & DNA Cleanup Kit (5 μg) (NEB, # T1030). The purified DNA sequence was then analyzed through Sanger sequencing (GENEWIZ from Azenta ...
-
bioRxiv - Molecular Biology 2022Quote: ... the products were amplified by PCR using Phusion High-Fidelity PCR Master Mix with HF Buffer (New England Biolabs) with 200 nM of Illumina multiplex primer and 200 nM of Illumina index barcode primers (98°C for 10 sec pre-denaturation followed by 12 cycles of 98°C for 5 sec ...
-
bioRxiv - Zoology 2023Quote: ... We performed 5 PCR enrichment with 15 cycles (30 ng DNA input, NEB Phusion High-Fidelity PCR Master Mix) for each library to increase fragment diversity ...
-
bioRxiv - Genomics 2022Quote: ... Purified genomic DNA was directly amplified by 22 cycles of PCR using NEBNext Ultra II Q5 PCR MasterMix (NEB). Sequencing was performed on a NovaSeq 6000 (Illumina ...
-
bioRxiv - Plant Biology 2024Quote: ... The sequencing libraries were prepared by PCR amplification using the NEBNext PCR master mix (New England Biolabs, no. M0541S). The amplified libraries were purified with AMPure beads (Beckman Coulter ...
-
bioRxiv - Microbiology 2024Quote: ... PCR products were then either purified directly using Monarch PCR and DNA Cleanup Kit (New England Biolabs, Hitchin, UK), or if more than one band was present extracted from 1% TAE gel using the QIAquick Gel Extraction Kit (Qiagen ...
-
bioRxiv - Cancer Biology 2023Quote: ... Addgene cat#52961) by PCR amplification using NEB Next High-Fidelity PCR Master Mix (New England Biolabs, cat#M0541S). The PCR product was digested with Esp3I (BsmBI ...
-
bioRxiv - Cancer Biology 2024Quote: ... PCR reactions contained 2 μl cDNA in 50 μl PCR reaction with Phusion Hot Start Flex (New England Biolabs). Reaction conditions ...
-
bioRxiv - Genetics 2023Quote: ... The PCR reaction was performed using NEBNext® High-Fidelity 2× PCR Master Mix (New England BioLabs, catalog #M0541) with a reaction volume of 10 ul including 5 ul 2× PCR Master Mix ...
-
bioRxiv - Microbiology 2023Quote: ... The 30 μl PCR reaction system contained 15 μl of Phusion high-fidelity PCR Master Mix (New England Biolabs), 0.2 μM forward and reverse primers ...
-
bioRxiv - Cancer Biology 2023Quote: ... Resulting amplicons from the second PCR were column purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs; NEB) to remove genomic DNA and first round PCR product ...
-
bioRxiv - Cancer Biology 2023Quote: ... Resulting amplicons from the second PCR were column purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs; NEB) to remove genomic DNA and first round PCR product ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... beads were directly used for off-bead PCR amplification using the Phusion High-Fidelity PCR kit (New England Biolabs) for 30 cycles with primers from library amplification (Rd1 ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... and successful PCR products were cleaned up using Monarch® PCR and DNA Clean-Up Kit (New England Biolabs) as per kit instructions ...
-
bioRxiv - Genomics 2023Quote: ... All PCR reactions were performed with 15 μL of Phusion® High-Fidelity PCR Master Mix (New England Biolabs), 0.2 μM of forward and reverse primers ...
-
bioRxiv - Cell Biology 2023Quote: The novel entry modules created for this study were cloned by insertion of PCR-amplified sequences in pJet1.3 or pMiniT 2.0 following manufacturer’s instructions (CloneJET PCR Cloning Kit, Thermo Scientific; NEB® PCR Cloning Kit, NEB). BsaI recognition sites followed by module-specific overhangs were added to each primer used to amplify entry sequences ...
-
bioRxiv - Plant Biology 2022Quote: ... were analyzed by Quantitative real time PCR (qRT PCR) using Luna® Universal qPCR Master Mix (New England BioLabs) in a QuantStudio® 5 Real-Time PCR System (Thermo Fisher Scientific ...
-
bioRxiv - Genetics 2023Quote: ... The target loci were PCR amplified in 30 PCR cycles using Q5 High-Fidelity DNA Polymerase (New England Biolabs), locus-specific primer pairs (Supplementary Information ...
-
bioRxiv - Genomics 2023Quote: ... ATAC-seq libraries were prepared by ∼8 cycles of PCR using NEBNext High Fidelity 2X PCR Master Mix (NEB) and primers containing Illumina barcodes (Buenrostro et al ...
-
bioRxiv - Genomics 2023Quote: ... Accessible DNA libraries were amplified by PCR using NEBNext High-Fidelity PCR Master Mix (New England Biolabs, Ipswich, MA), custom Nextera PCR primers as previously described (29) ...
-
bioRxiv - Genomics 2024Quote: ... cDNA was PCR amplified using the NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs, Cat. No. M0541) with the following forward and reverse primers ...
-
bioRxiv - Genomics 2024Quote: ... Tagmented DNA was PCR amplified using NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs, Cat. No. M0541) with indexed primers (CAAGCAGAAGACGGCATACGAGATNNNNNNNNNNGTCTCGTGGGCTCGG and AATGATACGGCGACCACCGAGATCTACACNNNNNNNNNNACACTCTTTCCCTACACGACGCTCTTCCGATCT ...
-
bioRxiv - Synthetic Biology 2024Quote: All transcription templates were generated using PCR amplification (Phusion High-Fidelity PCR Kit, New England Biolabs, catalog no. E0553) of an oligo that includes a T7 RNAP promoter ...
-
bioRxiv - Developmental Biology 2021Quote: ... the synthesized sgB was injected at 300ng/µL after 5-minute incubation at RT with Cas9 protein (NEB) at 2µM final in 20mM Hepes-NaOH pH 7.5 ...
-
bioRxiv - Physiology 2020Quote: Input RNA (50-100 ng/µL) was reverse-transcribed using LunaScript® RT SuperMix Kit (New England BioLabs) or TaqMan MicroRNA Reverse Transcription Kit (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2020Quote: ... cDNAs were synthesized from RNA using the LunaScript™ RT SuperMix Kit from New England BioLabs (NEB, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2020Quote: Each reverse transcription (RT) reaction was performed using 50 ng total RNA in 1 mM dNTPs (NEB N0447S), 10% DMSO (Fisher ...