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Citations for New England Biolabs :
2051 - 2100 of 7434 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Plant Trans-Golgi Network/Early Endosome pH regulation requires Cation Chloride Cotransporter (CCC1)bioRxiv - Plant Biology 2021Quote: ... following a Phusion® PCR protocol (NEB). The PCR product and the binary plasmid pMDC43 were subsequently cut with the restriction enzymes HindIII-HF and KpnI-HF to remove the 2×35S promoter ...
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR amplification followed the recommended protocol (NEB) with 12 PCR cycles and a 50 sec ...
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bioRxiv - Biochemistry 2021Quote: ... Colony PCR (OneTaq polymerase, New England Biolabs) with the primer pairs clp2_KO_A–pLSV101_seq rev and pLSV101_seq fwd–clpP2_KO_D (see Tables 2 and 3 ...
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bioRxiv - Synthetic Biology 2021Quote: ... and Monarch PCR & DNA Cleanup Kit (NEB) were used in this study for DNA purification.
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bioRxiv - Microbiology 2020Quote: ... Colony PCRs used OneTaq DNA Polymerase (NEB). Restriction enzymes (NEB ...
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bioRxiv - Developmental Biology 2022Quote: ... we used Q5 PCR mix (NEB, M0492L) to amplify the libraries for high-throughput sequencing in Illumina platform.
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bioRxiv - Molecular Biology 2022Quote: ... PCR with taq DNA polymerase (NEB M0273S) followed by agarose gel electrophoresis was used to screen for the presence of the 3xFLAG tag and the YPWM-AAAA mutation ...
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bioRxiv - Cancer Biology 2022Quote: ... PCR-amplified (NEBNext Ultra II Q5; NEB) using primers DCF01 5’-CTTGTGGAAAGGACGAAACACCG-3’ and DCR03 5’-CCTAGGAACAGCGGTTTAAAAAAGC-3’ and subjected to single-end 75 bp (SE75 ...
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CRISPR-Cas9-mediated knockout of CYP79D1 and CYP79D2 in cassava attenuates toxic cyanogen productionbioRxiv - Plant Biology 2021Quote: ... the Monarch PCR & DNA Cleanup Kit (NEB), or SPRI magnetic nucleic acid purification beads (UC Berkeley DNA Sequencing Facility) ...
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bioRxiv - Microbiology 2022Quote: ... using colony OneTaq PCR (New England Biolabs). For protease activity ...
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bioRxiv - Synthetic Biology 2020Quote: ... PCR products were treated with DpnI (NEB) and purified using a gel extraction kit (Qiagen) ...
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bioRxiv - Immunology 2021Quote: ... PCR amplicons were digested using PvuII (NEB) restriction enzyme at 37°C for 1 hour and were run on an agarose gel ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR products were amplified with Phusion (NEB), following the manufacturer’s specifications ...
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bioRxiv - Synthetic Biology 2021Quote: ... Monarch PCR & DNA Cleanup Kit (NEB #T1030L) was used to purify the fragments and they were all adjusted to the concentration of 100 nM to use for active learning experiments.
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bioRxiv - Biochemistry 2021Quote: ... Colony PCR (OneTaq polymerase, New England Biolabs) with the respective primer pairs clp_A–pLSV101_seq rev and pLSV101_seq fwd–clp_D (Table 2 ...
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bioRxiv - Developmental Biology 2020Quote: ... PCR amplicons were column purified (NEB, T1030) and used as a template for in vitro transcription reactions with a mMESSAGE mMACHINE® T7 Transcription Kit (Ambion ...
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bioRxiv - Microbiology 2021Quote: ... PCR fragments were gel purified (NEB T1020L) and cloned into pMiniT 2.0 vector (NEB E1202S) ...
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bioRxiv - Genomics 2020Quote: ... PCR was performed using Phusion HF (NEB) and TruPE PCR primers (Illumina) ...
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bioRxiv - Genetics 2020Quote: ... PCR was performed with Q5 polymerase (NEB) following manufacturer’s instructions with 10 PCR cycles (primers listed in Table S1) ...
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bioRxiv - Microbiology 2021Quote: ... PCR with Q5 High Fidelity Polymerase (NEB) was used to generate insert and backbone DNA ...
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bioRxiv - Neuroscience 2022Quote: ... Phusion HF PCR Master Mix (1x, BioLabs), water (6.42 µL) ...
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bioRxiv - Biochemistry 2022Quote: ... PCR product was digested with DPNI (NEB), followed by treatment with T4 Polynucleotide kinase (PNK ...
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bioRxiv - Developmental Biology 2022Quote: ... and a second round of PCR (NEB Q5 polymerase ...
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bioRxiv - Molecular Biology 2022Quote: ... mTurbo was amplified by pfusion PCR (NEB) using Ti833/Ti834 as primers and 3xHA-miniTurboNLS_pCDNA3 (addgene #107172 ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were digested by ExoI (NEB) to remove excess of oligos and purified using Qiagen PCR purification kit ...
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bioRxiv - Genomics 2024Quote: ... 20uL 2X NEBnext PCR master mix (NEB) was added ...
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bioRxiv - Microbiology 2024Quote: ... PCR was conducted using Q5 polymerase (NEB) following manufacturer’s guidelines ...
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bioRxiv - Genetics 2023Quote: ... All libraries were PCR amplified (NEB M0544S) for 5 cycles ...
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bioRxiv - Cell Biology 2023Quote: ... PCR was performed using Phusion polymerase (NEB). Unique Fluidigm sample barcodes were added using Fast Start High Fidelity PCR System (Roche) ...
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bioRxiv - Synthetic Biology 2023Quote: ... PCR products and NdeI/XhoI (NEB, USA) digested pAN001 vector DNA were assembled using Gibson assembly standard protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Q5 PCR Master Mix (New England BioLabs) was used for PCR reactions ...
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bioRxiv - Microbiology 2023Quote: ... Monarch® PCR & DNA Cleanup Kit (NEB), or Gene Jet gel extraction kit (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... and Monarch PCR Cleanup Kit (NEB, T1030). PCR primers used ...
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bioRxiv - Biochemistry 2023Quote: ... PCR was performed using Phusion polymerase (NEB) and primers that add Illumina adapter sequences and a 6 bp identifier sequence used to distinguish cell populations ...
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bioRxiv - Bioengineering 2023Quote: ... PCR with Q5 polymerase from NEB (M0493S), followed by HiFi assembly (NEB E2621S ...
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bioRxiv - Developmental Biology 2024Quote: ... Monarch PCR purification kit (Biolabs, New England) used to purify the resulting amplified cDNA constructs ...
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bioRxiv - Cell Biology 2024Quote: ... amplified by PCR (15 cycles, Phusion, NEB) and cloned into the XhoI and EcoRI sites of pSC2 as previously described(38 ...
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bioRxiv - Systems Biology 2024Quote: ... PCR also introduced specific i5 (NEB, 7780) and N8 (Vazyme ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR fragment was digested using NotI (NEB) and ligated with NotI-digested pCAG-Puromycin plasmid using T4 DNA ligase (NEB ...
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bioRxiv - Molecular Biology 2019Quote: ... and subjected as an input for library preparation process using the NEBNext® Fast DNA Library Prep Set for Ion Torrent™ (New England Biolabs Inc. USA) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... in order to generate sufficient material to construct ChIP-seq sequencing libraries using a NEBNext® ChIP-seq Library Pre Reagent Set for Illumina® (New England Biolabs, Ipswich, MA). DNA sequencing was performed on an Illumina Hiseq2500 platform (Novogene ...
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bioRxiv - Cancer Biology 2021Quote: ... The libraries were then prepared using NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina (#E6240, New England Biolabs, Ipswich, Massachusetts, USA) and sequenced on an Illumina HiSeq 2000 sequencer (RRID:SCR_020132 ...
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bioRxiv - Molecular Biology 2019Quote: ... The restriction digest was set up directly afterwards and performed overnight at 37°C with agitation (750 r.p.m.) with HindIII (NEB; 20 µl of 20 U/µl). Using biotin-14-dCTP (Life Technologies) ...
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bioRxiv - Molecular Biology 2020Quote: ... The DAP gDNA library was prepared using the kit from NEBNext® DNA Library Prep Master Mix Set for Illumina® (NEB #E6040S/L). ZmIBH1-1 was fused to HaloTag using the kit from pFN19K HaloTag T7 SP6 Flexi Vecto (cat#G184A ...
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bioRxiv - Evolutionary Biology 2023Quote: ... DNA was converted into double stranded blunt-end libraries with Illumina-specific adapters REF using the NEBNext DNA Sample Prep Master Mix Set 2 (E6070S - New England Biolabs Inc., Beverly, MA, USA) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... All samples were then homogenized mechanically using a Monarch® Pestle Set single-use microtube pestle (New England Biolabs® Inc., Ipswich, MA, USA). Homogenization was performed both prior to ...
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bioRxiv - Cell Biology 2020Quote: Resulting amplicons from the second PCR were column purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs; NEB) to remove genomic DNA and first round PCR product ...
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bioRxiv - Cell Biology 2020Quote: Resulting amplicons from the second PCR were column purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs; NEB) to remove genomic DNA and first round PCR product ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Polymerase chain reaction (PCR) products and plasmids were purified using Monarch® PCR & DNA Cleanup Kit (New England Biolabs) and Sanger sequencing was performed by Eurofins Genomics ...
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bioRxiv - Molecular Biology 2022Quote: ... The CXCL8 promoter was amplified by PCR with Phusion® High-Fidelity PCR Master Mix with HF Buffer (NEB) according to the manufacturer’s instruction with the following primers: