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Citations for New England Biolabs :
1901 - 1950 of 7434 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... PCR amplification was performed with Phusion High-Fidelity PCR Master Mix with HF buffer (NEB, MA, USA) and a unique indexed RNA PCR primer (Illumina ...
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bioRxiv - Cancer Biology 2023Quote: ... Quantitive PCR and limited-cycle PCR was performed using NEBNext Ultra II master mix (New England Biolabs), SYBR™ green and 0.5 µM of sample-specific P5/P7 barcoded primer mix (New England Biolabs) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCR products were generated using the OneTaq Quick-Load 2x PCR Master Mix with Standard Buffer (NEB) in accordance with manufacturer instructions with C ...
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bioRxiv - Bioengineering 2023Quote: ... PCR was performed using Phusion High-Fidelity PCR Master Mix with HF Buffer (New England Biolabs®) or RedTaq DNA Polymerase 2X Master Mix (VWR Life Science ...
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bioRxiv - Developmental Biology 2024Quote: ... libraries were generated by PCR amplification using NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs) and purified using Agencourt AMPure XP magnetic beads (Beckman Coulter ...
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bioRxiv - Cancer Biology 2024Quote: ... The PCR products were purified using the Monarch DNA and PCR Cleaning kit (New England Biolabs #T1030S) and templates were digested using Dnp1 (New England Biolabs #R0176) ...
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bioRxiv - Molecular Biology 2020Quote: ... Briefly 60 ng ±10% of total RNA was subjected to small RNA library preparation by using the NEBNext Multiplex Small RNA Library Prep Set 1 and 2 for Illumina (NEB, Ipswich, MA, USA) kit according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Small RNA-seq libraries were prepared using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs, Ipswich, United States). Small RNAs were enriched using bead-based size selection ...
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bioRxiv - Immunology 2019Quote: ... Pooled DNA (100 ng) was fragmented and barcoded using the NEBNext→ Fast DNA Fragmentation and Library Prep Set for Ion Torrent™ (NEB Inc, UK) and IonXpress Barcode Adapters kit (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... PCR reaction mix was set up in duplicate for each sample with Q5 Hot Start High-Fidelity 2x Master Mix (New England Biolabs, Ipswich, MA, USA) with annealing conditions of 54°C for 25 cycles ...
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bioRxiv - Genomics 2020Quote: ... genomic DNA libraries were constructed for sequencing on Illumina platforms using the NEBNext® DNA Sample Prep Master Mix Set 1 (New England Biolabs, Ipswich, MA). First ...
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bioRxiv - Molecular Biology 2019Quote: ... Libraries were prepared from 1 μg of total RNA using the NEB Next Multiplex Small RNA Library Prep Set for Illumina (NEB #E7300S and #E7580S) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: A 25 μL PCR master mix was set up as follows: 2.5 μL Standard Taq (Mg-free) Reaction Buffer (10x) (B9015 New England BioLabs Inc. Ipswich MA USA), 2.0 μL dNTP mix (10mM total ...
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bioRxiv - Immunology 2021Quote: Small RNA libraries were generated from each RNA sample at TCAG (Toronto, Canada) using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs, Ipswich, United States) and sequenced on an Illumina HiSeq 2500 rapid-run flow cell to generate 50 nucleotide (nt ...
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bioRxiv - Plant Biology 2022Quote: ... from leaves was isolated for small RNA library construction using NEB Next® Multiplex Small RNA Library Prep Set for Illumina® (NEB, USA) following manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2022Quote: ... One microgram of total RNA per biological replicate was employed for the construction of 50 bp single-end (SE) reads using NEBNext® Multiplex Small RNA Library Prep Set for Illumina™ (New England Biolabs, Inc.). Lastly ...
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bioRxiv - Neuroscience 2024Quote: ... all the extracted mRNA was used to create cDNA libraries with the help of the NEBNext mRNA Library PrepReagent Set for Illumina (New England Biolabs, Ipswich, MA USA). Sequencing of the cDNA libraries was performed on the Illumina HiSeq 2500 platform (Illumina Sequencing ...
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bioRxiv - Genomics 2022Quote: Small RNA libraries were prepared for each sample using the NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (NEB, USA) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2024Quote: ... the RNA pellet was resuspended in 3’ ligation reaction buffer 1x and subjected to library preparation using the NEBNext® Small RNA Library Prep Set for Illumina® (NEB, #E7330S). Specific primers for V ...
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bioRxiv - Cancer Biology 2021Quote: ... The regular PCR was performed using Phusion High-Fidelity PCR Master Mix with HF Buffer (New England Biolabs). The PCR products that targeted exon18-10 and exon9-10 were then combined for each sample and run on 2% agarose gel ...
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bioRxiv - Developmental Biology 2021Quote: ... The PCR reactions (total 30 μL) included 15 μL PhusionR High-Fidelity PCR Master Mix (New England Biolabs), 0.2 mM primers ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The PCR products from all reactions were pooled and purified with the Monarch PCR & DNA Cleanup Kit (NEB) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2020Quote: ... Sequenced PCR products showing overlapping peaks in their chromatograms were cloned using NEB PCR cloning kit (NEB #E1202S) and then sequenced to define the identity of mutations carried by F1 heterozygous animals ...
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bioRxiv - Microbiology 2019Quote: ... the PCR products were purified using Monarch® PCR & DNA Cleanup Kit (5 μg; New England Biolabs GmbH). For the second PCR ...
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bioRxiv - Genomics 2021Quote: ... The PCR reaction consisted of 25 µL NEBNext Hi-Fidelity 2x PCR Master Mix (New England Biolabs NEB.M0541S), 7.5 µL H2O ...
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bioRxiv - Genomics 2021Quote: ... The PCR reaction consisted of 25 µL NEBNext Hi-Fidelity 2x PCR Master Mix (New England Biolabs NEB.M0541S), 7.5 µL H2O ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR amplification was performed in 1X Phusion® High-Fidelity PCR Master Mix with HF Buffer (NEB M0531) and PCR cycling at 98°C for 30s ...
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bioRxiv - Plant Biology 2021Quote: ... transposed DNA was PCR amplified with 12 cycles using Next High-Fidelity 2×PCR Master Mix (NEB, M0541) with Nextera DNA CD Index primers ...
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bioRxiv - Biophysics 2022Quote: ... PCR cleanup was carried out on each amplicon sample using the Monarch PCR cleanup kit (New England Biolabs). The amplicons were then dual-indexed for paired-end read Illumina sequencing using Nextera primers N701 and N702 for the PEL and SUP of the 5.5 h time point and N705 and N706 for the PEL and SUP of the 9.0 h time point ...
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bioRxiv - Immunology 2022Quote: ... Final amplification PCR was performed as described before by adding 8 µl of 2 x PCR MM (NEB) with number of cycles according to qPCR (usually Cq values were around 20) ...
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bioRxiv - Microbiology 2021Quote: ... Reverse transcription and qRT-PCR analysis was performed by Luna universal one- step qRT-PCR kit (NEB # E3005L) with primers list in Table 9.
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bioRxiv - Neuroscience 2020Quote: ... PCR amplification was performed for 15 cycli using the NEBNext High-Fidelity 2X PCR Master Mix (NEB, M0541), followed by a 0.8x beads clean-up ...
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bioRxiv - Microbiology 2019Quote: ... All the PCR reactions were carried out with Phusion® High-Fidelity PCR Master Mix (New England Biolabs). The libraries generated with TruSeq DNA PCR-Free Sample Preparation Kit were sequenced using paired-end Illumina sequencing (2 × 250 bp ...
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bioRxiv - Synthetic Biology 2019Quote: ... PCR reactions were performed with the Phusion High-Fidelity PCR Master Mix with HF buffer (New England Biolabs). All inserts were verified by sequencing (GENEWIZ) ...
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bioRxiv - Genomics 2020Quote: ... The final PCR amplification was performed using 2X Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB) and final 0.1 μM of PE1.0 and corresponding multiplex PE2.0_MTX (Table S3) ...
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bioRxiv - Molecular Biology 2021Quote: ... Tandem PCR was performed by Q5 PCR (0.5 μl Q5® HiFi DNA Polymerase (New England Biolabs, M0491), 1x Q5 reaction buffer ...
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bioRxiv - Biochemistry 2021Quote: ... 11) with 15 cycles of PCR using NEBNext® Q5® Hot Start HiFi PCR Master Mix (NEB), which allowed to add Gap Repair recombination sequences for the cloning in Gal4-AD prey plasmid pP7 ...
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bioRxiv - Immunology 2019Quote: ... the PCR product was subjected PCR using Q5® Hot Start High-Fidelity 2X Master Mix (NEB; M0494S) in two separate reactions to amplify the KK10 or the KY9 epitope ...
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bioRxiv - Immunology 2020Quote: ... and amplified by PCR for 11 cycles using NEBNext High Fidelity 2x PCR Master Mix (New England Biolabs). PCR products were purified using a PCR Purification Kit (Qiagen ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Inverse PCR was performed using NEBNext high-fidelity 2X PCR master mix (New England Biolabs, catalog no. M0541). DNA was purified with SPRISelect beads ...
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bioRxiv - Genomics 2021Quote: ... we performed an initial PCR for locus-specific amplicon enrichment using NEBNext 2X High Fidelity PCR MM (NEB) and 5’-biotinylated stub adapter primers specific to appropriate genomic regions to be interrogated (Figure 5-table supplement 1) ...
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bioRxiv - Immunology 2022Quote: ... and amplified by PCR for 11 cycles using NEBNext High Fidelity 2x PCR Master Mix (New England Biolabs). PCR products were purified using a PCR Purification Kit (Qiagen ...
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bioRxiv - Microbiology 2022Quote: ... PCR amplification of the dhodh locus was performed using Phusion High-Fidelity PCR Master Mix (New England BioLabs) as per the protocol ...
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bioRxiv - Microbiology 2022Quote: ... Tagmented DNA was amplified by PCR using 1x NEBNext Hi-Fidelity PCR Master Mix (New England Biolabs, NEB), using Nextera Index i5 and i7 series PCR primers ...
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bioRxiv - Microbiology 2022Quote: ... Tagmented DNA was amplified by PCR using 1x NEBNext Hi-Fidelity PCR Master Mix (New England Biolabs, NEB), using Nextera Index i5 and i7 series PCR primers ...
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bioRxiv - Molecular Biology 2022Quote: ... tagmented DNA was PCR amplified using 1x Phusion® High-Fidelity PCR Master Mix with GC Buffer (NEB) and 1.25 μM i5 and i7 PCR primers (Nextera® Index Kit (Illumina) ...
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bioRxiv - Synthetic Biology 2024Quote: ... and PCR products were purified with Monarch PCR and DNA Cleanup kit (New England Biolabs, Ipswich, Massachusetts, USA).
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bioRxiv - Zoology 2024Quote: ... PCR products were purified with a Monarch PCR & DNA clean-up kit (New England Biolabs, Whitby, ON, Canada) and used as template to reamplify the products to incorporate a Kozak translation initiation sequence prior to the start codon (Kozak ...
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bioRxiv - Microbiology 2024Quote: ... The PCR product of the selected deletion mutant was purified using Monarch PCR and DNA cleanup kit (NEB) and sent for sequencing at Eurofins Genomics LLC (Louisville ...
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bioRxiv - Microbiology 2023Quote: ... PCR reactions were undertaken using sporocarp extractions using the PHusion High-Fidelity PCR Master Mix (New England Biolabs). Libraries were generated using the TruSeq DNA PCR-Free Prep Kit (Illumina ...