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Citations for New England Biolabs :
1901 - 1950 of 4002 citations for Hexadecanoic acid reaction products with tetraethylenepentamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Digested products were purified utilizing a spin column and ligated by T4 DNA ligase (New England Biolabs) for 1 hour at room temperature following manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting plasmid products were transformed into NEB 10-beta electrocompetent cells (New England Biolabs, Ipswich, MA) and purified using a ZymoPURE Midiprep Kit (Zymo Research ...
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bioRxiv - Biochemistry 2024Quote: ... Nicking mutagenesis plasmid products were transformed into NEB 10-beta electrocompetent cells (New England Biolabs, Ipswich, MA) and purified using a ZymoPURE Midiprep Kit (Zymo Research ...
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bioRxiv - Developmental Biology 2024Quote: ... The amplified pools were subjected to PCR product purification by Monarch PCR and DNA Cleanup Kit (NEB). The purified cDNAs were cloned by NEB PCR Cloning Kit and individual clones were isolated and sequenced (Azenta Life Sciences).
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bioRxiv - Cancer Biology 2024Quote: ... DNA fragments were then cloned into a lentiviral expression vector with a Gibson assembly product (NEB, E2611S). DNA sequencing was performed to confirm accuracy of the vector ...
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bioRxiv - Bioengineering 2024Quote: ... 5 μL of PCR product from the inner primer amplification was cleaned using 5μL Exonuclease I (NEB) and 2μL rSAP (NEB) ...
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bioRxiv - Developmental Biology 2024Quote: ... After PCR amplification or plasmid digestion product was purified with Monarch PCR clean-up kit (NEB, #T1030s) and used to produce the DIG-labeled single-stranded RNA probes using the DIG RNA labeling kit (Roche ...
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bioRxiv - Microbiology 2023Quote: ... PCR product was assembled with EcoRI-linearized pBAD L24 using NEBuilder HiFi DNA Assemly Master Mix (NEB). Strep-Tag II and His-tag C-terminally modified SAMase coding plasmids were constructed in a similar manner ...
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bioRxiv - Plant Biology 2023Quote: ... The PCR products were purified using Phusion High-Fidelity DNA polymerase (New England Biolabs, Frankfurt a.M., Germany) and subse-quently ligated into the vector system pCR-Blunt II-TOPO via the Zero Blunt™ TOPO™ PCR cloning kit (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2023Quote: ... The PCR products containing the geneticin128-795-TtrpCand LB border were digested using AgeI (New England Biolabs) followed by a ligation reaction ...
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bioRxiv - Genomics 2023Quote: ... The PCR products were purified using the Monarch PCR & DNA Cleanup Kit (New England Biolabs, no. T1030L). In vitro transcription of pegRNAs and gRNAs was performed using the HiScribe SP6 RNA Synthesis Kit (New England Biolabs ...
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bioRxiv - Genetics 2022Quote: ... The fusion product was conventionally cloned into pCS2FA using the FseI and AscI sites (enzymes from NEB). This placed the fusion product upstream of the SV40 polyA signal sequence ...
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TAD border deletion at the Kit locus causes tissue-specific ectopic activation of a neighboring genebioRxiv - Genetics 2022Quote: ... The ligation products were reverse crosslinked at 65°C overnight with proteinase K (New England Biolabs, P8107S) and DNA was extracted with phenol-chloroform ...
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bioRxiv - Cell Biology 2023Quote: ... The PCR products were digested with DpnI and assembled with Gibson assembly master mix (New England Biolabs) according to the manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: ... All products were amplified using Q5-High Fidelity DNA polymerase according to the manufacturer (New England Biolabs). The PCR reactions containing each part of the amplified plasmid backbone were treated with DpnI to remove the methylated parent plasmid ...
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bioRxiv - Developmental Biology 2023Quote: ... The products were cloned into pRA638 using the NEBuilder HiFi DNA Assembly Master Mix (NEB, Ipswich, MA). All primers are listed in Supplemental Material (File S1 ...
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bioRxiv - Zoology 2023Quote: ... The amplified products were measured using a 100 base pair (bp) DNA ladder (New England Biolabs Inc.) at specific bands corresponding to their base pairs for the two bacterial species.
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bioRxiv - Cell Biology 2023Quote: ... PCR amplified products were screened by restriction digest analysis using the CviAII restriction enzyme (New England Biolabs). Positive candidates were sequenced to identify and confirm indels (Figure S6).
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bioRxiv - Synthetic Biology 2023Quote: ... 1 μg RCA product was restricted by employing 10 U nicking endonuclease Nb.BbvCI (New England Biolabs, R0631L) in a 50 μL reaction volume with 1x rCutsmart buffer for 1 h at 37 °C ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCR products were generated using the OneTaq Quick-Load 2x PCR Master Mix with Standard Buffer (NEB) in accordance with manufacturer instructions with C ...
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bioRxiv - Genomics 2023Quote: ... The product was purified with 1.8× Ampure XP beads and eluted in 8 μL elution buffer (NEB). The DNA was denatured by addition of 2 μL 0.1 M freshly diluted NaOH and incubation at 50°C for 10min ...
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bioRxiv - Biophysics 2023Quote: ... The PCR product was spin column purified and then digested with BssSI-v2 and PpuMI (NEB R0560) in order to form the 6,481 bp center segment with 4 or 3 bp overhangs on the ends ...
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bioRxiv - Cancer Biology 2024Quote: ... The PCR products were purified using the Monarch DNA and PCR Cleaning kit (New England Biolabs #T1030S) and templates were digested using Dnp1 (New England Biolabs #R0176) ...
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bioRxiv - Microbiology 2024Quote: ... amplified plasmid and insert PCR products were assembled using NEBuilder HiFi DNA Master Mix (New England Biolabs) following manufacturer’s protocols and transformed into Escherchia coli strain S-17 by electroporation for maintenance and replication ...
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bioRxiv - Cell Biology 2024Quote: ... The mutated product was transformed into chemically competent Escherichia coli (C2987H) through a High Efficiency Transformation (NEB). Colonies were isolated via growth on selective media (LB carbenicillin+) ...
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bioRxiv - Systems Biology 2024Quote: ... Step 6.2n product) was in-vitro transcribed (IVT) with the NEB Quick T7 HiScribe Kit (NEB #E2050) in 25 µL reactions per manufacturer protocol ...
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bioRxiv - Microbiology 2024Quote: ... The resulting PCR products were checked by gel-electrophoresis and purified (Monarch PCR&DNA Cleanup Kit, NEB) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Digested insert (PCR product) and vector (pQE60) were gel purified and ligated by T4 DNA ligase(NEB) in 10X ligation buffer overnight at 16°C ...
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bioRxiv - Neuroscience 2024Quote: ... DNA products were then digested overnight using 0.1 U/µL of restriction enzyme BglII (New England Biolabs). Discrimination between genotypes was performed on a gel electrophoresis according to the presence or absence of WT (undigested bands ...
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bioRxiv - Microbiology 2024Quote: ... The PCR products were then cloned into PstI digested pTS1 with the HiFi DNA assembly kit (NEB). Only the first 138 bp of the lrs1 gene were deleted ...
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bioRxiv - Neuroscience 2024Quote: ... 200 ng of the amplified gDNA region product and 1 μM EnGen® Sau Cas9 (#M0654, NEB), according to the manufacturer ...
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bioRxiv - Microbiology 2024Quote: ... PCR products were gel purified and Golden Gate assembly43 was performed with PaqCI and T4 ligase (NEB). Products of the Golden Gate assembly were transformed into 5-alpha competent E ...
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bioRxiv - Synthetic Biology 2024Quote: ... The PCR amplified products were gel extracted and assembled via NEBuilder HiFi DNA assembly master mix (NEB). The assembled product was transformed into eBSD35 ...
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bioRxiv - Biochemistry 2024Quote: ... the PCR product was purified using NucleoSpin Gel and PCR Clean-up (New England Biolabs, MA, USA).
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bioRxiv - Biochemistry 2024Quote: ... The crude circRNA product was on a silica based-column (Monarch RNA cleanup kit, 50 µg, NEB) followed by RP-HPLC ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the purified PCR products were subcloned into pcDNA3.1 vector pretreated with NheI and BamHI (New England Biolabs) to generate pcDNA3.1-HA-STBD1 expression plasmids for each of the four proteins ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The purified PCR products were subcloned into pET32a vector pretreated with BamHI and HindIII (New England Biolabs) using ClonExpress® II One Step Cloning Kit (Vazyme ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Eluted purified PCR products (45μl in nuclease-free water) were digested with a lambda exonuclease (NEB M0262L) and accompanying reaction buffer for 20 minutes at 37°C (total reaction volume 50μl) ...
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bioRxiv - Synthetic Biology 2021Quote: ... 50-µL PCR reactions contained 1x Phusion HF buffer (New England Biolabs; Ipswich, MA, USA), 200 µM dNTPs ...
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bioRxiv - Biophysics 2021Quote: ... 25 μL of PCR reaction was mixed with 1 μL of DpnI (NEB, cat#R0176) at 37°C for 4 hours ...
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bioRxiv - Genetics 2020Quote: ... and then the PCR reactions were treated with 200 U/ml of Exonuclease I (NEB) for 1 hour at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: ... with subsequent end repair/dA-tailing reaction using Klenow Fragment (3’-5’ exo-) (NEB M0212S) and ligation with Illumina sequencing adapters using T4 DNA ligase (NEB) ...
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bioRxiv - Genomics 2021Quote: ... PCR reactions were cleaned up using 0.9X NEBNext Sample purification beads (E7137AA, New England Biolabs) and eluted in 25μL of 0.1X TE ...
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bioRxiv - Microbiology 2020Quote: ... a single-pot reaction was set up containing T4 ligase (10 000U) and buffer (NEB), SapI (NEB ...
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bioRxiv - Genomics 2020Quote: ... followed by a fill-in reaction with adapter sequences (Bst DNA polymerase, New England Biolabs; 30 min ...
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bioRxiv - Plant Biology 2022Quote: ... or by the Gibson reaction using the NEBuilder HiFi assembly master mix (New England Biolabs). E ...
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bioRxiv - Microbiology 2022Quote: ... A PCR reaction (30 cycles) was run with Phusion® High-Fidelity DNA polymerase (NEB). The primer pairs im126/im127 and im137/im138 were used to target the reference gene pta (74 ...
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bioRxiv - Molecular Biology 2021Quote: ... Single step PCR was performed using a One-Taq™ PCR reaction kit (NEB, USA) with 30 amplification cycles and 2 ng of original circular-DNA extract as the DNA template ...
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bioRxiv - Molecular Biology 2021Quote: ... The debranching reaction was stopped by incubating with 0.8 units Proteinase K (New England Biolabs) for 5 minutes at 37 °C ...
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Interaction of the Xanthomonas effectors XopQ and XopX results in induction of rice immune responsesbioRxiv - Molecular Biology 2020Quote: ... Ligation reactions for cloning in pHM1 were carried out using T4 DNA ligase (NEB, Massachusetts).