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Citations for New England Biolabs :
1801 - 1850 of 4002 citations for Hexadecanoic acid reaction products with tetraethylenepentamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... and IVT reactions were set up according to the HiScribe™ T7 (E2040S, NEB) or Sp6 (E2070S ...
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bioRxiv - Cell Biology 2022Quote: ... The cloning reactions were performed using the Gibson Assembly Master Mix (New England Biolabs). Site-directed mutagenesis was performed with the Quik-Change kit (Agilent) ...
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bioRxiv - Genomics 2022Quote: ... An isothermal extension reaction mixture was made from 35μl NEBuffer2 (10×, New England Biolabs), 12.5μl Klenow Fragment (5,000U/ml ...
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bioRxiv - Genomics 2022Quote: ... and used as the template for in vitro transcription reaction (New England Biolabs E2040S) performed according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... sheared gDNA is first end-repaired in 20 μL reactions using Quick CIP (NEB) and following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Gibson reactions were carried out according to manufacturer’s recommendations (NEB, Gibson Assembly master mix). This was transformed into electrocompetent E ...
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bioRxiv - Microbiology 2022Quote: ... was combined in a 30 μL reaction with 160 μM SAM (New England Biolabs), 1X Cutsmart buffer (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... the ligation reaction was treated by 10 U of Exonuclease I enzymes (NEB, USA) for 1 hour at 37 °C for degradation of non-circularized library ...
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bioRxiv - Microbiology 2023Quote: ... All PCR reactions were performed using Q5 high-fidelity DNA polymerase (NEB, cat. #M0491S) according to the manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2023Quote: ... Real time reactions were performed using Luna® Universal qPCR Master Mix from NEB. Oligonucleotides were used to detect GFP-VAMP (56 and 57 ...
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bioRxiv - Microbiology 2023Quote: ... The purified ligation reaction was transformed into NEB-10-Beta competent cells (NEB C3020K) and the lawns resulting from this transformation were pooled together and inoculated into LB broth supplemented with kanamycin ...
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bioRxiv - Bioengineering 2022Quote: ... 10 μL PCR reactions were set-up using 2 μL 5x HF Buffer (NEB), 0.5 μL 10 mM dNTPs (NEB) ...
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bioRxiv - Cancer Biology 2023Quote: ... A volume of 40 μl reaction mix containing 5 μl isothermal amplification buffer (NEB), 3 μl 100 mM MgSO4 ...
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bioRxiv - Genetics 2023Quote: ... PCR reactions were performed with Phusion High-Fidelity PCR Master Mix (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Restriction reactions were performed using High-Fidelity Restriction enzymes from New England Biolabs (NEB), by incubating 2 μg of DNA with 2U of each enzyme in the presence of 10X NEB CutSmart buffer ...
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bioRxiv - Microbiology 2023Quote: ... 20-μL reactions contained 2 μM enzyme and 250 μM NTPs (New England BioLabs) in reaction buffer with 50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Immunology 2022Quote: ... IgL: GCCGCGTACTTGTTGTTGC) in a 30 μl reaction with Q5 DNA polymerase (New England Biolabs). Cycling conditions were one cycle 98°C/30 s ...
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bioRxiv - Microbiology 2023Quote: ... Reactions were terminated with addition of 0.5 μL of Quick CIP (New England Biolabs) to remove terminal phosphate groups from unreacted nucleotides ...
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bioRxiv - Developmental Biology 2023Quote: ... On-Bead 5’ Decapping reaction was done using NEBuffer 2 buffer (NEB, B7002S ®). Off-Bead Reverse Transcription followed the instructions in SuperScript ® IV Reverse transcriptase kit ...
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bioRxiv - Cell Biology 2024Quote: ... Gibson reactions were set up with NEBuilder HiFi DNA Assembly Master Mix (NEB E2621), 50ng of linearized backbone ...
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bioRxiv - Microbiology 2024Quote: ... Five microliters were used in ampliication reactions using LongAmp® Taq DNA Polymerase (NEB) and primers ...
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bioRxiv - Bioengineering 2024Quote: Polymerase chain reaction (PCR) of constructs utilized for plasmid assembly were amplified by NEB Q5 Hot-Start 2X Mastermix according to the manufacturer’s protocol (New England Biolabs ...
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bioRxiv - Biochemistry 2024Quote: ... The assembly reaction was transformed into NEB 5-alpha competent cells (New England Biolabs). After the recovery step ...
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bioRxiv - Neuroscience 2024Quote: ... The ligation reactions were carried out using the NEBuilder HiFi DNA Assembly kit (NEB). The pENTR1A-h-syn-P-aSynA53T-mVenus plasmid was generated by site-directed mutagenesis using overlap extension PCR to introduce the A53T mutation ...
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bioRxiv - Microbiology 2024Quote: ... All PCR reactions were performed using Q5 High-fidelity DNA polymerase (New England Biolabs). Primers used for PCR reactions are listed in Table 1 ...
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bioRxiv - Genomics 2024Quote: ... One reaction of NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, #E7645) was used for each gDNA sample ...
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bioRxiv - Immunology 2024Quote: PCR reactions were performed using NEBNext® High-Fidelity 2X PCR Master Mix (NEB) under the conditions ...
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bioRxiv - Molecular Biology 2024Quote: ... if the reaction was not digested immediately by DpnI (New England Biolabs, Cat. R0176). For DpnI digestion ...
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bioRxiv - Microbiology 2024Quote: ... RNA was expressed overnight in 30 µL T7 transcription reactions (NEB # E2050S, includes DNAse) with 3 ng of either DNA template ...
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bioRxiv - Synthetic Biology 2024Quote: ... across 10 reactions) were first treated with NheI-HF (New England Biolabs, Cat# R3131L) overnight ...
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bioRxiv - Biochemistry 2024Quote: ... Reactions were stopped by adding 2µl of stop buffer (10mg.ml-1 proteinase K (NEB), 1% SDS ...
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bioRxiv - Molecular Biology 2024Quote: ... The dephosphorylation reaction was supplemented with 0.5 μl T4 PNK (NEB, Cat. No. M0201S), and the reaction was incubated for 25 minutes ...
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bioRxiv - Synthetic Biology 2024Quote: ... The PCR reactions were performed using Q5 High-Fidelity 2X PCR Master Mix (NEB) with optimized Tm and the PCR products were detected by 1% agarose gel.
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bioRxiv - Microbiology 2024Quote: ... The PCR reaction was set up using Q5 polymerase (New England Biolabs, Whitby, Canada) following the manufacturer’s recommendation ...
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bioRxiv - Physiology 2024Quote: ... the following reagents were added to each reaction: 1 mM dNTP (NEB, Cat. #N0447), 20 U RNase Inhibitor (NEB ...
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bioRxiv - Neuroscience 2024Quote: ... Each PCR reaction was subsequently supplemented with 5 μL of CutSmart Buffer (NEB B7204) and digested using 1μL DpnI restriction enzyme (NEB R0176 ...
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bioRxiv - Systems Biology 2024Quote: ... The eight reactions were combined into a single PCR clean-up column (NEB Monarch).
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bioRxiv - Synthetic Biology 2024Quote: ... Taq reactions were performed in a 1X Taq Standard Buffer (New England Biolabs, B9014) containing 10 mM Tris-HCl ...
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bioRxiv - Genomics 2024Quote: ... The nuclei were resuspended in 564 µL 1X GpC MTase reaction buffer (NEB, B0227) and equilibrated to 37°C ...
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bioRxiv - Genomics 2024Quote: ... The reaction was purified with 1x volume of NEBNext Sample Purification Beads (NEB #E7103) and eluted with 40 μL of 0.1xTE buffer ...
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bioRxiv - Genomics 2024Quote: ... One reaction of NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645) was used for each sample ...
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bioRxiv - Genomics 2024Quote: ... The digestion reaction was stopped by adding 5µl Proteinase K (NEB, catalog number: P8107) and incubation at 37°C for 30 min ...
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bioRxiv - Biochemistry 2024Quote: ... and 1600 Units of T7 RNA polymerase in T7 RNA Polymerase reaction buffer (NEB). Reactions were incubated at 37°C for 3 hours then digested with 64 units of RNase Free DNaseI (NEB ...
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bioRxiv - Synthetic Biology 2024Quote: ... across 3 reactions) were double digested with NheI-HF (New England Biolabs, Cat# R3131L) and MfeI-HF (New England Biolabs ...
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bioRxiv - Biochemistry 2024Quote: ... and then treated with the KLD reaction mix (New England Biolabs, Ipswich, Massachusetts, USA) to digest the template ...
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bioRxiv - Synthetic Biology 2024Quote: ... Oligos were double stranded across multiple low cycle PCR reactions using Q5 polymerase (NEB, Cat ...
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bioRxiv - Synthetic Biology 2024Quote: ... DNA assembly reactions were performed using NEBuilder® HiFi DNA Assembly Master Mix (NEB). Plasmid DNA was purified using the QIAprep Spin Miniprep Kit (Qiagen ...
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bioRxiv - Cancer Biology 2024Quote: ... XBP1 cDNAs were amplified in a PCR reaction containing 2X Phusion Master Mix (NEB). The forward primer 5’-GGAGTTAAGACAGCGCTTGGGGA-3’ and the reverse primer 5’-TGTTCTGGAGGGGTGACAACTGGG-3’ ...
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bioRxiv - Biochemistry 2024Quote: ... Each 50 μL reaction contained 25 μL Q5 High-Fidelity DNA Polymerase (NEB #M0494S), 2 μL oligo pool ...
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bioRxiv - Biochemistry 2024Quote: ... Each 50 μL reaction contained 25 μL Q5 High-Fidelity DNA Polymerase (NEB #M0494S), 2.5 μL each primer ...