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Citations for New England Biolabs :
1951 - 2000 of 3344 citations for PD 1 Mouse HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Annealed circles were ligated with the addition of 1 μL of T4 DNA ligase (NEB M0202S) at room temperature for ~4 hours ...
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bioRxiv - Genetics 2021Quote: ... for 1 hour at 37°C into pPB_U6::gRNA_EF1a::BFP-Puro digested with BlpI (NEB #R0585S) and BstXI (NEB #R0113S) ...
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bioRxiv - Neuroscience 2020Quote: ... After overnight incubation at 4°C in PBS-Perm with rabbit anti-GFP (1:1000, Biolabs) and mouse anti-MAP2 (1:2000 ...
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bioRxiv - Cell Biology 2021Quote: ... Sonicated salmon sperm DNA was then diluted to 1 mg/ml in Terminal Transferase buffer (NEB) with 0.25 mM CoCl2 ...
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bioRxiv - Biophysics 2021Quote: ... The cells were then incubated with the SNAP substrate (Alexa 647 – benzylguanine, 1 µM, NEB#S9136S) in 0.5% BSA + 1mM DTT at room temperature for 2 hours ...
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bioRxiv - Biophysics 2021Quote: ... before staining with SNAP dye buffer (1 μM BG-AF647 (catalog no. S9136S, New England Biolabs) and 1 μM dithiothreitol in 0.5% [w/v] bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2020Quote: ... Positive control slides were treated with 3 U/mL DNase-1 (New England Biolabs, Cat. M0303) for 10 minutes at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... and barcoded using NEBNext Multiplex Oligos for Illumina (Index Primers Set 1 & 2; New England Biolabs). Number of PCR cycles was calculated using a real-time qPCR-based approach (Lion et al. ...
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bioRxiv - Immunology 2022Quote: ... and 1 μg was used to generate dsRNA using a HiScribe T7 RNA synthesis kit (NEB). Reaction products were purified with the GeneJET RNA Purification Kit (ThermoFisher) ...
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bioRxiv - Microbiology 2022Quote: ... each chamber is filled for 30 min with a 1 mg/ml solution of Streptavidin (NEB) and then passivated with a blocking solution (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Physiology 2022Quote: ... and the DNA was digested for 1 h with I-SceI (New England BioLabs, Beverly, MA) at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... These were eluted and precipitated before ligation to 5’ adapters (1× T4 RNA ligase buffer (NEB), 0.5 µM p10IllLigup adapter ...
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bioRxiv - Plant Biology 2022Quote: ... We then ligated single-stranded rP5_RND adapters to 5’-ends with T4 RNA ligase 1 (NEB). Ligated RNAs were enriched and captured by oligo(dT ...
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bioRxiv - Biophysics 2022Quote: ... cells were incubated in dye solution (1 μM BG-AF647 (catalog no. S9136S, New England Biolabs), 1 mM DTT (catalog no ...
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bioRxiv - Cell Biology 2022Quote: ... Editing efficiency of bulk cell pools was initially confirmed by T7E1 (T7 Endonuclease 1, #M0302S, NEB) assay ...
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bioRxiv - Systems Biology 2023Quote: ... the flow cell was incubated with Exonuclease I mix (1 U/uL Exo I (NEB, M0293L) in 1x Exo I buffer ...
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bioRxiv - Molecular Biology 2023Quote: Endpoint PCR was performed in a 20 µL reaction volume containing 1× OneTaq Master Mix (NEB), 0.2 µM of each primer and 1 µL of 100-fold diluted cDNA ...
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bioRxiv - Developmental Biology 2024Quote: ... The sections were then incubated in a ligation mix (1× T4 Rnl2 reaction buffer (NEB, B0239SVIAL), 0.05 µM RCA primer ...
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bioRxiv - Molecular Biology 2024Quote: ... Genomic DNA (1 µg) was digested with the methylation-sensitive restriction enzyme HpaII (New England Biolabs) and analyzed by Southern hybridization with a specific biotin-labeled DNA probe (300 ng ...
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bioRxiv - Microbiology 2024Quote: ... MssI and XhoI restriction enzymes and a Quick-Load® 1 kb Extend DNA Ladder (NEB). Plasmid curing was confirmed by loss of pESI related antibiotic resistance ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µg of total RNA was reverse transcribed using LunaScript RT SuperMix Kit (New England Biolabs) according to the manufacturer’s instructions and 1 µl cDNA was used as a template for a 10 µl qPCR reaction ...
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bioRxiv - Microbiology 2024Quote: ... 1 μg of RNA was used for cDNA synthesis using the LunaScript RT SuperMix Kit (NEB) with and without reverse-transcriptase ...
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bioRxiv - Microbiology 2024Quote: ... two inverse PCRs were carried out using R3-p21 as a template and oligonucleotides Trip.A.For/Trip.A.Rev and Trip.B.For/Trip.B.Rev (Supplementary Table 1) using Phusion High-Fidelity DNA Polymerase (New England BioLabs) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: 10µg of a plasmid containing 807bp of Pcdh10 exon 1 sequence was linearized with SacII (NEB) in 1x CutSmart buffer (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... 5 units (2.5 μL) of RNase free DNAse I (NEW ENGLAND BioLabs, 2000 u. mL-1) and 100 μL of nuclease free water was added for every 10 μg of RNA ...
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bioRxiv - Genetics 2024Quote: ... then digested with a final concentration of 1 U/μL of DpnII (NEB cat no. R0543S) in 450 μL of 1X digestion reaction buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were mixed with 1/5th volume of 6X gel loading buffer (New England Biolabs®) and then electrophoresed in pairs alongside their undigested counterparts on an agarose gel as described below ...
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bioRxiv - Genetics 2024Quote: ... m7G(5’)ppp(5’)G RNA Cap Structure Analog (New England Biolabs, 4:1 to GTP) was supplemented to the in vitro transcription reaction ...
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bioRxiv - Biochemistry 2023Quote: ... diluted 1:10 in PBS-BSA (PBS supplemented with 0.4 mg/mL BSA (New England Biolabs)) ...
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bioRxiv - Genomics 2023Quote: 1-2 µg of extracted total RNA and polysomal RNA was treated with DNaseI (NEB, M0303) in a 100 µL reaction at 37 °C for 15 min ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was produced from ∼1 µg of RNA using M-MuLV Reverse Transcriptase (New England Biolabs). RTqPCR was performed on 1:10 dilutions of the cDNA using iTaq Universal SYBR Green Supermix (Bio Rad ...
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bioRxiv - Cancer Biology 2024Quote: RNAs were transcribed from 1 µg of PCR-amplified templates using T7 RNA polymerase (NEB M0251S) for 2 h at 37 °C according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... T4 RNA ligase 1 was mixed with splinted RNA in a 1x T4 Rnl buffer (NEB) supplemented with ATP (1 mM ...
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bioRxiv - Neuroscience 2023Quote: ... recombinant SYNJ2BP (0.5 µg) was dephosphorylated using 1 µl of calf intestinal alkaline phosphatase (CIP) (NEB) in 1x CutSmart Buffer in a total volume of 10 µl ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 μl 10 pM/μl and previously adenylated using the 5′ DNA adenylation kit (NEB, E2610S), and 1 μl T4 RNA ligase 2 truncated KQ (NEB M0373L) ...
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bioRxiv - Microbiology 2023Quote: ... 200 μM dNTPs and 2500 nM NEBNext Universal and Index Primer each (Primer Set 1, NEB). PCR products were purified by native PAGE and ethanol-precipitated ...
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bioRxiv - Molecular Biology 2022Quote: The cloning procedure was as follows: 1 µl of T4 DNA Ligase (400 U; NEB M0202), 2 µl of T4 DNA Ligase Buffer (NEB M0202) ...
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bioRxiv - Molecular Biology 2023Quote: ... 6 µl aliquots were 5’ adapter ligated (25 µl of 1× T4 RNA ligase buffer (NEB), 1 mM ATP ...
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bioRxiv - Microbiology 2023Quote: ... 2 µM each of gene- specific forward and reverse primers and 1 unit Phusion polymerase (NEB) were mixed in 1X HF Phusion buffer and 3% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... Pre-adenylated L3-1R-App 3’ adaptors were ligated using T4 RNA ligase 1 (M0204, NEB) for 75 min at 25°C ...
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bioRxiv - Plant Biology 2023Quote: ... 4 pmol of double-stranded DNA was labeled with 1 unit of Klenow fragment polymerase (NEB) and 8 pmol Cy5-dCTP (Cytiva ...
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bioRxiv - Cell Biology 2023Quote: ... and 62.5 μL of Lambda Protein Phosphatase (New England Biolabs, 5 U μg-1 of protein) were added with gentle mixing after each addition ...
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bioRxiv - Bioengineering 2023Quote: ... we used the NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) oligos (NEB E7600S). All sequencing was performed using an Illumina NextSeq 75 cycle high-output kit (Illumina 20024906 ...
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bioRxiv - Bioengineering 2023Quote: ... the AsCas12a-crRNA complexes were combined with 1 µL of binding buffer (New England BioLabs, #B6002S), 4.5 µL of biotinylated quencher probes ...
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bioRxiv - Genomics 2023Quote: ... and 1 uL of 10X T4 DNA ligase reaction buffer (New England Biolabs, Cat. No. B0202S) in a final volume of 10 uL ...
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bioRxiv - Molecular Biology 2023Quote: ... 200 ng of purified PCR product and 1 µl of NEB 2 Buffer (New England Biolabs) in 9.5 µl total volume were first denatured for 5 min at 95°C and then rehybridized by cooling the sample at a rate of 2°C/s to 85°C and a rate of 0.1°C to 25°C to obtain potential heteroduplex DNA ...
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bioRxiv - Biochemistry 2022Quote: ... 1 μL of the HMCESSRAP-DNA crosslinking reaction and 0.5 μL of APE1 (New England BioLabs) were added to 8.5 μL final reaction buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Two micrograms of total RNA was circularized by a T4 RNA Ligase 1 Kit (M0204. NEB) and then purified by TRIzol reagent followed by isopropanol precipitation ...
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bioRxiv - Cell Biology 2023Quote: ... diluted 1:100 and used for the ligation using the Quick Ligation Kit (New England Biolabs). Specific base pair substitutions were introduced with site directed mutagenesis by polymerase chain reaction (PCR) ...
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bioRxiv - Molecular Biology 2023Quote: ... The fragmented RNA was then circularized with RNA ligase 1 in 20 µl reactions (NEB, M0204S) for 2 hours at 25°C after which the circularized RNA was purified with the Oligo Clean & Concentrator kit (D4061 ...