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Citations for New England Biolabs :
1851 - 1900 of 10000+ citations for PCR Genotyping kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... PCR products were digested with DpnI (New England Biolabs; Ref.: R0176S) to remove circular DNA ...
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bioRxiv - Genomics 2024Quote: ... Oligos were amplified through multiple PCR reactions (New England Biolabs [NEB] Q5 High-Fidelity 2X Master Mix ...
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bioRxiv - Genetics 2023Quote: ... PCR fragments were amplified using Phusion DNA polymerase (New England Biolabs) using the generic protocol and 30 s/Kb extension ...
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bioRxiv - Biochemistry 2024Quote: ... PCR reactions were carried out using Taq polymerase (New England Biolabs) and contained 2.5 µM gene-specific primers that flanked the intron ...
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bioRxiv - Genetics 2024Quote: ... DNA was PCR amplified with Q5 high fidelity DNA polymerase (NEB). The forward primer MitfaPCR-F binds to the mitfa genomic region upstream of the insertion site and the reverse primer 5’homology_amplify-R binds the p2A region of the insertion cassette ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were then amplified with Q5 PCR mix (New England Biolabs) for a total of 16-25 cycles ...
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bioRxiv - Systems Biology 2024Quote: ... We setup 50uL PCR reactions with 1x OneTaq GC Buffer (NEB), 400uM of additional MgSO_4 ...
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bioRxiv - Microbiology 2024Quote: ... PCR was performed using Q5 high-fidelity DNA polymerase (NEB; M0491L). The amplicon was treated with KLD enzyme mix (NEB ...
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bioRxiv - Neuroscience 2024Quote: ... All PCRs were performed using Q5 High-Fidelity DNA Polymerase (NEB). All plasmids were transformed into Escherichia coli Stable3 competent cells (NEB) ...
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bioRxiv - Immunology 2024Quote: ... The third PCR was performed with the Phusion hot Start (NEB) to add the Illumina adapter and index ...
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bioRxiv - Genomics 2024Quote: ... Genomically integrated guide sequences were amplified using NEBNext PCR polymerase (NEB) and staggered guide-specific primers (Supplementary Table 1 ...
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bioRxiv - Microbiology 2024Quote: ... DNA was PCR-amplified using Phusion polymerase (New England Biolabs; NEB) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... DNA was PCR-amplified using Phusion polymerase (New England Biolabs; NEB) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: The PCR product was digested with SfiI and NotI enzymes (NEB) and ligated into a pHEN2 vector modified with a triple c-Myc tag108 which was used to transform electrocompetent TG1 cells (Lucigen) ...
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bioRxiv - Cancer Biology 2023Quote: ... Duplex PCR was performed using Q5 Hotstart 2x Master Mix (NEB) with final primer concentrations of 0.5uM ...
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bioRxiv - Synthetic Biology 2023Quote: ... by reverse PCR and blunt end ligation with KLD (NEB M0554S). Subsequently ...
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bioRxiv - Synthetic Biology 2023Quote: ... 4-5 cycles of PCR with OneTaq polymerase (New England Biolabs) was performed using the forward (pBPS_fwr ...
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bioRxiv - Cell Biology 2023Quote: ... 25 µL NEBNext HiFi 2× PCR Master Mix (New England BioLabs) was added to the DNA mixture ...
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bioRxiv - Developmental Biology 2023Quote: ... The resulting PCR product was treated with T4 PNK (NEB M0201S) and T4 DNA ligase (NEB M0202S) ...
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bioRxiv - Cell Biology 2023Quote: ... gBlocks were PCR-amplified with Q5 High-Fidelity DNA Polymerase (NEB) using the forward primer T7-Kozak-start-fwd and the reverse primers HA-PolyA-rev (for PNPLA3-HA constructs ...
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bioRxiv - Plant Biology 2023Quote: ... All PCRs were carried out using the Q5 DNA polymerase (NEB) on Arabidopsis cDNA libraries or genomic DNA ...
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bioRxiv - Synthetic Biology 2023Quote: ... with DNA fragments obtained by PCR with the Q5 polymerase (NEB) [21] ...
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bioRxiv - Microbiology 2023Quote: ... Multiplex PCR reactions were performed using Q5 polymerase (New England Biolabs) under the following cycling conditions on a Bio-Rad T100 thermal cycler ...
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bioRxiv - Microbiology 2023Quote: Routine PCR was performed using Q5 High-fidelity Mastermix (NEB M0492L). PCR purification and gel extraction was performed using Macherey-Nagel Kit cat ...
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bioRxiv - Microbiology 2023Quote: ... PCR amplifications were performed with Q5 DNA polymerase (NEB, MA, USA) and assembly was performed using the HiFi DNA assembly master mix (NEB) ...
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bioRxiv - Genomics 2023Quote: ... we performed PCR with Q5 Hot Start High-Fidelity (NEB M0494S), column purification with QIAquick PCR Purification Kit (Qiagen 28104) ...
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bioRxiv - Bioengineering 2023Quote: ... PCR amplified (Q5 HotStart DNA polymerase (NEB, Cat. No./ID: M0493L)) ...
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bioRxiv - Genetics 2023Quote: ... Purified PCR products were then treated with Exonuclease I (NEB M0568L) and purified using 1× AMPure XP beads (Beckman Coulter A63881) ...
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bioRxiv - Biochemistry 2023Quote: ... was PCR amplified with mutagenic primer pairs using Q5 polymerase (NEB) (Supplementary Table S1) ...
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bioRxiv - Genomics 2023Quote: ... Purified PCR products were then treated with Exonuclease I (NEB, M0568L) and purified using 1× AMPure XP beads (Beckman Coulter ...
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bioRxiv - Genomics 2023Quote: ... The PCR reaction was carried out using 2X LongAmp Taq (NEB) with the following PCR parameters 94°C for 3 minutes ...
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bioRxiv - Immunology 2023Quote: ... 2.5µl Evagreen and 2x Q5 PCR Master mix (NEB, Catalog #50492L) and end point PCR was performed using following program ...
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bioRxiv - Bioengineering 2023Quote: ... and amplified with standard PCR using Phusion polymerase (New England BioLabs). Purified mutant DNA and linearized plasmid were electroporated into EBY100 yeast ...
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bioRxiv - Genetics 2023Quote: ... All PCRs were performed using the high-fidelity Q5 polymerase (NEB) as per manufacture instructions ...
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bioRxiv - Genomics 2023Quote: ... Purified PCR products were then treated with Exonuclease I (NEB, M0568L) and purified using 1× AMPure XP beads (Beckman Coulter ...
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bioRxiv - Genomics 2023Quote: ... using touchdown PCR with Phusion High-Fidelity Polymerase (NEB, no. M0530S) and primers containing partial sequencing adapters (Supplementary Table 6) ...
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bioRxiv - Cell Biology 2023Quote: ... The amplified PCR product was restriction digested using EcoRI/XhoI (NEB). Simultaneously ...
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bioRxiv - Cancer Biology 2023Quote: ... and NEBNext HiFi 2x PCR Master mix (New England Biolabs M0541). The PCR conditions were ...
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bioRxiv - Neuroscience 2023Quote: ... the PCR reaction mixture consisted of 2x Q5 HotStart Mastermix (NEB), 500nM primer-F ...
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bioRxiv - Molecular Biology 2023Quote: ... All PCRs were performed using Q5 High-Fidelity DNA polymerase (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Atp10A DNA products were detected via PCR (Q5 DNA Polymerase, NEB) followed by gel electrophoresis ...
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bioRxiv - Molecular Biology 2023Quote: ... All PCRs were performed using Q5 High-Fidelity Master Mix (NEB). All Gibson assemblies were performed using NEBuilder HiFi DNA Assembly (NEB).
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bioRxiv - Neuroscience 2023Quote: ... All PCR reactions were performed with Q5 polymerase (New England Biolabs) and cleaned up using Nucleospin Gel and PCR spin columns (Macherey-Nagel) ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products were purified and digested using the appropriate enzymes (NEB) as described by Alsford S ...
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bioRxiv - Developmental Biology 2023Quote: ... Clones were screened by PCR on extracted genomic DNA (NEB, T3010L) using the primer pair listed in Supplementary Table 2.
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bioRxiv - Developmental Biology 2023Quote: ... The repair plasmids were either synthesized by Genscript Inc or by assembling PCR fragments with NEBuilder (New England Biolabs). Typical repair plasmids had homology arms of 500 bp to 1000bp ...
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bioRxiv - Synthetic Biology 2023Quote: All PCRs were done using Q5 2X Master Mix (NEB, M0492L). Primers were designed on Benchling (https://benchling.com/ ...
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bioRxiv - Plant Biology 2023Quote: ... followed by PCR using Q5® high-fidelity DNA polymerase (NEB) with specific primers (Table S2) ...
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bioRxiv - Synthetic Biology 2023Quote: ... PCR reactions were carried out using Phusion Master Mix from NEB in a final volume of 25 µL for diagnostic PCRs ...
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bioRxiv - Immunology 2023Quote: ... PCR products were digested with NheI and XhoI (New England Biolabs), gel-purified (Qiagen cat ...