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Citations for New England Biolabs :
1801 - 1850 of 10000+ citations for PCR Genotyping kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... 4-5 cycles of PCR with OneTaq polymerase (New England Biolabs) was performed using the forward (pBPS_fwr ...
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bioRxiv - Cell Biology 2023Quote: ... 25 µL NEBNext HiFi 2× PCR Master Mix (New England BioLabs) was added to the DNA mixture ...
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bioRxiv - Developmental Biology 2023Quote: ... The resulting PCR product was treated with T4 PNK (NEB M0201S) and T4 DNA ligase (NEB M0202S) ...
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bioRxiv - Cell Biology 2023Quote: ... gBlocks were PCR-amplified with Q5 High-Fidelity DNA Polymerase (NEB) using the forward primer T7-Kozak-start-fwd and the reverse primers HA-PolyA-rev (for PNPLA3-HA constructs ...
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bioRxiv - Genetics 2024Quote: ... DNA was PCR amplified with Q5 high fidelity DNA polymerase (NEB). The forward primer MitfaPCR-F binds to the mitfa genomic region upstream of the insertion site and the reverse primer 5’homology_amplify-R binds the p2A region of the insertion cassette ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were then amplified with Q5 PCR mix (New England Biolabs) for a total of 16-25 cycles ...
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bioRxiv - Systems Biology 2024Quote: ... We setup 50uL PCR reactions with 1x OneTaq GC Buffer (NEB), 400uM of additional MgSO_4 ...
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bioRxiv - Microbiology 2024Quote: ... PCR was performed using Q5 high-fidelity DNA polymerase (NEB; M0491L). The amplicon was treated with KLD enzyme mix (NEB ...
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bioRxiv - Neuroscience 2024Quote: ... All PCRs were performed using Q5 High-Fidelity DNA Polymerase (NEB). All plasmids were transformed into Escherichia coli Stable3 competent cells (NEB) ...
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bioRxiv - Genomics 2024Quote: ... Oligos were amplified through multiple PCR reactions (New England Biolabs [NEB] Q5 High-Fidelity 2X Master Mix ...
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bioRxiv - Genetics 2023Quote: ... PCR fragments were amplified using Phusion DNA polymerase (New England Biolabs) using the generic protocol and 30 s/Kb extension ...
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bioRxiv - Biochemistry 2024Quote: ... PCR reactions were carried out using Taq polymerase (New England Biolabs) and contained 2.5 µM gene-specific primers that flanked the intron ...
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bioRxiv - Immunology 2024Quote: ... The third PCR was performed with the Phusion hot Start (NEB) to add the Illumina adapter and index ...
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bioRxiv - Genomics 2024Quote: ... Genomically integrated guide sequences were amplified using NEBNext PCR polymerase (NEB) and staggered guide-specific primers (Supplementary Table 1 ...
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bioRxiv - Microbiology 2024Quote: ... DNA was PCR-amplified using Phusion polymerase (New England Biolabs; NEB) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... DNA was PCR-amplified using Phusion polymerase (New England Biolabs; NEB) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: The PCR product was digested with SfiI and NotI enzymes (NEB) and ligated into a pHEN2 vector modified with a triple c-Myc tag108 which was used to transform electrocompetent TG1 cells (Lucigen) ...
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bioRxiv - Plant Biology 2023Quote: ... All PCRs were carried out using the Q5 DNA polymerase (NEB) on Arabidopsis cDNA libraries or genomic DNA ...
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bioRxiv - Genomics 2022Quote: Ribosomal RNA depletion kit (rRNA Depletion Kit, E6310L, NEB)
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bioRxiv - Cancer Biology 2021Quote: ... PCR was conducted using Q5 HotStart High Fidelity Polymerase (NEB, Ipswich, MA) using forward primer ...
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bioRxiv - Genetics 2019Quote: ... All PCR-amplifications were conducted using Phusion High-Fidelity DNA Polymerase (NEB). C ...
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bioRxiv - Microbiology 2020Quote: ... 25 μL 2x NEBnext High-Fidelity PCR Master Mix (New England Biolabs) and 10 μL nuclease-free distilled H2O (ThermoFisher Scientific) ...
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bioRxiv - Developmental Biology 2021Quote: ... Oligos were amplified twice by PCR (Phusion High-Fidelity DNA Polymerase, NEB) following manufactural instruction ...
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bioRxiv - Developmental Biology 2021Quote: ... Obtained PCR products with digested vector were assembled by Gibson assembly (NEB) following manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2021Quote: ... Ten PCR cycles with Phusion High-Fidelity DNA polymerase (New England Biolabs) were used for library amplification with indexed adaptors ...
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bioRxiv - Cell Biology 2019Quote: ... where the PCR product was treated with DpnI (New England BioLabs, R0176S) then purified with Monarch PCR & DNA Purification Kit (New England BioLabs ...
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bioRxiv - Cell Biology 2020Quote: ... by fusion PCR using Q5 High-Fidelity DNA Polymerase (New England Biolabs) and used as a homologous repair template ...
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bioRxiv - Genetics 2021Quote: ... All PCRs were performed using the Q5 High-Fidelity DNA Polymerase (NEB). The amplicons were purified using a Universal DNA Purification Kit ...
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bioRxiv - Genetics 2021Quote: ... The resulting PCR products were restriction-digested with EagI (New England Biolabs) for pDEW201-P1iraD and ApaI (New England Biolabs ...
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bioRxiv - Genetics 2021Quote: ... The resulting PCR products were restriction digested with EagI (New England Biolabs) for pDEW201-P1iraD and ApaI (New England Biolabs ...
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bioRxiv - Genetics 2021Quote: Bacterial colony PCR with high fidelity DNA polymerase Phusion (New England Biolabs) was used to obtain the wild type alleles of holC and yoaA for construction of GAL4 fusions for the yeast two hybrid analysis as previously described (16) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Barcodes were amplified by 19 cycles of PCR using Phusion polymerase (NEB) and primers that add Illumina adapter sequences and an 8 bp identifier sequence used to distinguish libraries and time points ...
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bioRxiv - Microbiology 2021Quote: ... The resulting cDNA was PCR amplified with Q5 Hight Fidelity Polymerase (NEB) using LInker-2-partial (5′-TTAGGTGTTGGGTTGGGCCA-3′ ...
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bioRxiv - Genomics 2021Quote: ... The PCR products were digested with restriction enzymes AscI (New England Biolabs) and EcoRV (New England Biolabs ...
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bioRxiv - Developmental Biology 2021Quote: ... sgRNA was generated by PCR amplification using the Phusion Polymerase (NEB, M0530S) following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and mixed with the PCR amplicon in Gibson Assembly Master Mix (NEB; protocol as described by the manufacturer) ...
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bioRxiv - Developmental Biology 2020Quote: ... The purified PCR fragments were digested by SalI/FseI restriction enzymes (NEB) and LV-H2B-GFP backbone was also dephosphorylated (CIP ...
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bioRxiv - Developmental Biology 2020Quote: ... and to screen clones PCR products were digested using HF-HindIII (NEB). Enzyme digests were separated by electrophoresis and genotypes distinguished by fragment banding patterns (wild type ...
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bioRxiv - Synthetic Biology 2022Quote: ... Eight separate 50 μL PCR reactions were set up with Q5 (NEB) using 2.5 μL of the diluted midiprep as the template and with the primers Orange_NGS_F and R ...
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bioRxiv - Microbiology 2022Quote: ... was digested with EcoRV and PCR products inserted by Gibson Assembly (NEB) as previously described ...
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bioRxiv - Neuroscience 2021Quote: ... and Phusion High Fidelity PCR MasterMix (New England Biolabs, Ipswich, MA, USA) and amplified by 1 cycle of 72 °C for 5 min and 98 °C for 30 sec ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR was performed using Q5® High-Fidelity DNA Polymerases (NEB #M0491) and primers F01/R01 (10 ng oligos ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA was PCR amplified for 14 cycles by using LongAmp Taq (NEB). ONT adaptor ligation was performed using T4 DNA ligase (NEB) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Candidate cis-regulatory regions were amplified by PCR with OneTaq polymerase (NEB) and cloned into the pCR8 plasmid (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... PCR was performed with Taq 2X Master Mix (New England BioLabs, Maine), following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: Purified gDNA fragments were then PCR amplified (NEBNext Ultra II Q5, NEB) with FLI1F and FLIR primers in PCR1 (denaturation at 98°C for 30 seconds ...
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bioRxiv - Synthetic Biology 2019Quote: ... DNA was amplified using Phusion High-Fidelity PCR Master Mix (NEB, M0531S) and the following conditions ...
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bioRxiv - Genetics 2019Quote: ... Each oligonucleotide was amplified by PCR (Q5 High-Fidelity DNA Polymerase, NEB) for 15 cycles ...
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bioRxiv - Biophysics 2019Quote: ... PCR reactions were set up using 2x Q5 mastermix (NEB, cat# M0492), 10 ng of plasmid template ...
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bioRxiv - Microbiology 2019Quote: ... the two PCR products were purified and then digested with AarI (NEB) enzyme at 37 C for 5 hrs ...