Labshake search
Citations for New England Biolabs :
1751 - 1800 of 3576 citations for Vanin 1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... 1 μl BSA (20 mg/ml) (New England Biolabs Inc., Ipswich, MA, USA), 10 μl Brilliant III Ultra-Fast SYBR® Green Low ROX qPCR Master Mix (Agilent Technologies ...
-
bioRxiv - Molecular Biology 2020Quote: ... PCR round 1 and 2 were performed using Q5-High Fidelity polymerase (NEB) for 6 (FS231 and FS232 ...
-
bioRxiv - Cancer Biology 2021Quote: ... 10 μL 400 U μl-1 T4 DNA Ligase (NEB, high concentration formula) and 664 μL H2O and incubated for 120 mins at 23°C with 300 rpm slow rotation ...
-
bioRxiv - Genomics 2022Quote: ... We ligated a 3’ adapter ligation using T4 RNA Ligase 1 (NEB, M0204L). We performed a second bead binding followed by a 5’ decapping with RppH (NEB ...
-
bioRxiv - Immunology 2022Quote: ... Restriction digestion was quenched by adding 1 μl proteinase K (New England Biolabs) and incubation for 2 mins at 37°C ...
-
bioRxiv - Genomics 2022Quote: ... and NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1) (NEB) according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... pcDNA3.1.mNG2(1-10) was generated through gibson assembly (NEBuilder, New England Biolabs) of a pcDNA3.1 vector (Thermo Fisher Scientific) ...
-
bioRxiv - Biochemistry 2022Quote: ... Reactions were stopped by the addition of 1 μL of Proteinase K (NEB) and incubated at room temperature for 15 minutes ...
-
bioRxiv - Bioengineering 2022Quote: ... NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) oligos (NEB, E7600S), and an Illumina NextSeq 550 75 cycle high-output kit (Illumina ...
-
bioRxiv - Bioengineering 2022Quote: ... cas12a (1 μM) and ATP are purchased from NEB (New England Biolabs Inc). SYBR Green II dye ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were preserved in a 1:3 glacial acetic acid: methanol (Biolabs-chemicals) solution and karyotyped using g-banding.
-
bioRxiv - Cell Biology 2022Quote: ... with 10 mM 10x Adenosine 5’-Triphosphate (1:10, #P0756S, New England Biolabs). The reaction was incubated in a thermocycler for 37 °C for 30 min ...
-
bioRxiv - Developmental Biology 2021Quote: ... and injected into 1-cell stage zebrafish embryos together with I-SceI (NEB). Only embryos exhibiting low mosaic sfGFP expression were grown to adulthood ...
-
bioRxiv - Cell Biology 2021Quote: ... the cells were labelled with 1 μM SNAP-Surface 549 (New England Biolabs) for 15 minutes at 37°C ...
-
bioRxiv - Neuroscience 2021Quote: ... The PCR reaction was DpnI digested (New England Biolabs, 37°C, 1 h) and column purified (Macherey-Nagel ...
-
bioRxiv - Microbiology 2021Quote: ... 1× Phusion HF buffer and 0.4 U Phusion high fidelity DNA polymerase (NEB). PCR was conducted in a DNA Engine Tetrad 2 thermal cycler (Bio-Rad ...
-
bioRxiv - Molecular Biology 2019Quote: ... The mixture further contained 1 mM of each ribonucleotide (rNTPs, New England Biolabs), 0.4 u/µl NxGen RNase inhibitor (Lucigen) ...
-
bioRxiv - Plant Biology 2021Quote: ... 1 x Protease Inhibitor Cocktail) with appropriate amount of MNase (New England Biolabs) and incubated at 37 °C for 10 min ...
-
bioRxiv - Cancer Biology 2020Quote: ... and E-cadherin (24E10, Cell Signaling Technology, NEB, Frankfurt, Germany, #3195, 1:400) were used for immunohistochemistry (IHC ...
-
bioRxiv - Genetics 2022Quote: ... then digested with 1 μL of ApeKI (New England BioLabs, Ipswitch, MA, USA). This restriction enzyme recognizes a 5 bp degenerate sequence GCWGC ...
-
bioRxiv - Molecular Biology 2019Quote: ... 1 ul RNaseOUT and 1ul T4 RNA ligase 2 truncated K227Q (NEB M0351). Samples were then washed twice with PNK buffer ...
-
bioRxiv - Biophysics 2019Quote: ... before staining in SNAP dye buffer (1 μM BG-AF647 [New England Biolabs; #S9136S] ...
-
bioRxiv - Molecular Biology 2019Quote: ... The reaction was stopped by addition of 1 unit apyrase (New England Biolabs) per 100 μl reaction volume and incubation at 30°C for 10 min (sample t=0) ...
-
bioRxiv - Immunology 2020Quote: ... 1 mM EDTA) and phosphorylated using T4 Polynucleotide Kinase (New England BioLabs M0201L) according to manufacturer recommendations ...
-
bioRxiv - Molecular Biology 2020Quote: ... Plasmids (1 ug) were linearized using BamHI restriction enzyme (NEB, Ipswich, MA, USA), column-purified using the DNA Clean & Concentrator −5 Kit (Zymo Research Corporation ...
-
bioRxiv - Molecular Biology 2020Quote: ... 0.1 mM SAM and 1 μl Vaccinia capping enzyme (10 units/μl, NEB). The reaction was incubated at 37 °C for 1 h and the capped RNA was purified by phenol chloroform extraction and ethanol precipitation at −20 °C overnight ...
-
bioRxiv - Genomics 2020Quote: ... 8 μL of 5 M NaCL and 1 μL of RNase A (NEB) were added to every 200 μL of eluate and to the WCE samples (diluted to 200 μL with Elution buffer) ...
-
bioRxiv - Microbiology 2021Quote: ... The RT reaction was treated with 1 μl RNase H (New England Biolabs) and incubated at 37°C for 20 min to remove RNA ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Quick-Load 1 kb Extend DNA ladder or Low Range PFG Marker (NEB).
-
bioRxiv - Cell Biology 2019Quote: ... Chromatin was then solubilized by DNase I digestion (1 unit, New England Biolabs) in CSK buffer with Complete Protease Inhibitor for 15 min at 37 °C ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... 1 µg of purified DNA was fragmented using dsDNA Fragmentase (New England Biolabs). Then ...
-
bioRxiv - Cell Biology 2021Quote: ... containing 2□ μl of 1× NEB Next Cell Lysis Buffer (New England Biolabs). FACS sorting was performed with a BD Influx sorter (BD Biosciences ...
-
bioRxiv - Genomics 2021Quote: ... 115 nL of DpnI mix (1× NEB CutSmart buffer, 0.1 U NEB DpnI) was added ...
-
bioRxiv - Genomics 2021Quote: ... 115 nL of DpnI mix (1× NEB CutSmart buffer, 0.1 U NEB DpnI) was added ...
-
bioRxiv - Genomics 2021Quote: ssDNA-specific Digestion: 20µL cfDNA was combined with 3µL Exonuclease 1 (NEB, M0293S), 3µL 10x Exo 1 Buffer ...
-
bioRxiv - Molecular Biology 2021Quote: ... 1% SDS and 1.6 U/µl of proteinase K (New England Biolabs # P8107S) to reverse the crosslinks ...
-
bioRxiv - Biochemistry 2021Quote: ... The reaction was stopped by addition of 1 μL proteinase K (NEB, P8107S), followed by 15 min incubation at room temperature and addition of 20% formaldehyde) ...
-
bioRxiv - Systems Biology 2021Quote: ... NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) oligos (NEB E7600S), and an Illumina NextSeq 550 75 cycle high-output kit (Illumina 20024906) ...
-
bioRxiv - Developmental Biology 2021Quote: ... The purified RNA was dephosphorylated with 1 µL of T4 polynucleotide kinase (NEB) in 1x T4 PNK buffer for 1 h at 37 °C ...
-
bioRxiv - Plant Biology 2022Quote: ... 87 ng template DNA and 1-units Phusion DNA Polymerase (NEB, Evry, France). The PCR product was cloned using the hot fusion method (Fu et al. ...
-
bioRxiv - Biophysics 2021Quote: ... by adding 1 µl (20 units) of BamHI restriction enzyme (New England Biolabs) to 1 µg DNA ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were treated with 1 μM SNAP-cell 647-SiR (New England Biolabs) for 30 minutes ...
-
bioRxiv - Genomics 2021Quote: ... 20% PEG8000 and 20 U T4 RNA Ligase 1 (New England Biolabs, M0204) at 16°C for 12 hours.
-
bioRxiv - Genomics 2020Quote: ... and 1 μL of Phi29 (Catalog number: M0269L Vendor: New England Biolabs Inc). The combined products are cleaned at a 0.5x bead ratio and eluted in 10 μl of nuclease-free water.
-
bioRxiv - Biophysics 2020Quote: ... 1 µl of Universal nuclease (Pierce; 125U) and 10 of DNaseI (NEB; 20U) were added to the sample and incubate for 10 min at RT ...
-
bioRxiv - Genomics 2020Quote: ... The reaction mix included 1 μl of 10x poly(A) polymerase buffer (NEB), 0.1 mM of ATP ...
-
bioRxiv - Molecular Biology 2021Quote: ... The digested nuclei were ligated with 1 μl of T4 DNA ligase (NEB) in 100 μl of 1 × T4 ligation buffer for 24 h at 16°C ...
-
bioRxiv - Molecular Biology 2021Quote: ... RNA 5’ adaptor was ligated using T4 RNA Ligase 1 - high concentration (NEB) and 10 mM ATP ...
-
bioRxiv - Immunology 2020Quote: ... 5µl Quick-Load Purple 1 kb DNA ladder (New England Biolabs, Ipswich, Massachusetts) was used as reference ...
-
Glucocorticoid receptor collaborates with pioneer factors and AP-1 to execute genome-wide regulationbioRxiv - Genomics 2021Quote: ... 1.35 μL MNase (New England Biolabs, diluted 1:10 in MNase reaction buffer) was added to the resuspended chromatin and incubated at 37°C ...