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Citations for New England Biolabs :
1851 - 1900 of 3576 citations for Vanin 1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... 160 µL of 40 U µL-1 Taq DNA ligase (New England Biolabs) and 700 µL of PCR-grade H2O ...
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bioRxiv - Molecular Biology 2023Quote: ... 39 µL of qPCR mix containing 1× Phusion mix (New England Biolabs, M0531S), 0.125 µM P6 Tall v4 primer ...
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bioRxiv - Genetics 2023Quote: ... PCR-1 and PCR-2 amplicons were digested with DpnI (NEB Cat#R0176S) for 2 hours at 37°C to remove the YCp50-WT_PKR template ...
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bioRxiv - Genomics 2023Quote: ... were added to the reaction with T4 DNA Polymerase (1 unit; NEB, M0203) and incubated at 12°C for 15 min ...
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bioRxiv - Genomics 2023Quote: ... Beads were re-suspended in 30μl of 1 × NEB Buffer2 (NEB Cat#7002S) containing 9U of T4 DNA polymerase (NEB ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and treated with 1 µL (20 units) of DpnI (New England Biolabs, R0176L) for 1 h at 37°C before being purified on magnetic beads ...
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bioRxiv - Microbiology 2023Quote: ... followed by incubation for 1 hr with 4 units of DNase I (NEB) at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... 100 ng/µL injection mix (70 ng/µL 1 kb DNA ladder (NEB), 30 ng/µL plasmid of interest ...
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bioRxiv - Systems Biology 2023Quote: ... primers of the NEBNext dual primer set 1 (New England Biolabs, Ipswitch, MA) were used ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 founder with the L424V was determined by restriction digest with MseI (NEB) and Sanger sequence analysis ...
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bioRxiv - Cell Biology 2024Quote: ... then 1 μL Endo H and 2.5 μL GlycoBuffer 3 (New England Biolabs) was added and incubated for 1 hour at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... and 1/10th volume of DNAse I reaction buffer (NEB cat. no. B0303S) were added to 1 mL of phage lysate and incubated at 37°C for 30 min followed by a 60 min incubation at 65°C ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 mM phosphoenolpyruvate and 0.15 mg/ml bovine serum albumin (New England Biolabs), in a final volume of 15 µl ...
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bioRxiv - Systems Biology 2024Quote: ... then digested for 1 hour at 37 °C using SacI (New England Biolabs). This linearized plasmid was used for Gibson assembly with an insert composed of a 248 base pair region of PBS2 amplified from genomic DNA ...
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bioRxiv - Genetics 2024Quote: ... PCR-1 and PCR-2 amplicons were digested with DpnI (NEB Cat#R0176S) for 2 hours at 37°C to remove the MSp509_PKR-WT template DNA ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µl of tailing mix (0.05 µl Terminal Transferase (NEB, cat. no. M0315), 0.1 µl 10x Cutsmart buffer ...
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bioRxiv - Biochemistry 2021Quote: Plastin constructs (Supplemenatary Table 1) were cloned using NEBuilder (New England Biolabs, Ipswich, MA) into pcDNA3.1 with mEmerald fused at the C-termini ...
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bioRxiv - Genetics 2021Quote: ... Electroporated bacteria were immediately split into eight 1 mL aliquots of SOC (NEB, B9020S) and recovered for 1 h at 37 °C then independently expanded in 20 mL of LB supplemented with 100 μg/mL of carbenicillin (EMD ...
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bioRxiv - Genomics 2020Quote: ... the chromatin was collected and resuspended in 200 μL 1x NEBuffer 1 (NEB, B7001S) and 200 U NEB Exonuclease III (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... chromatin fraction was solubilized in Buffer A with 1:100 micrococcal nuclease enzyme (NEB) and incubated for 15 min at 37°C shaker.
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bioRxiv - Molecular Biology 2021Quote: ... and NEBNext Multiplex Oligos for Illumina Primer sets 1 and 2 (New England Biolabs). The libraries were amplified with 7 PCR cycles using Herculase II Fusion Polymerase kit (Agilent) ...
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bioRxiv - Molecular Biology 2021Quote: ... This was repeated with a 1:200 pA-Dam solution (∼60 NEB Dam units), followed by 2 wash steps ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 μL RT Adapter (RTA)(SQK-RNA002) and 2 μL T4 DNA Ligase (NEB) were mixed together and incubated under 25 °C for 10 min ...
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bioRxiv - Genomics 2020Quote: ... and followed by incubation with 3 μl (1 U/μl) of USER enzyme (NEB) at 37°C for 20 min ...
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bioRxiv - Molecular Biology 2021Quote: ... Component A contained 1 μL of Bst 3.0 DNA polymerase (Cat. No. M0374L, NEB), 2.5 μL of 10 × isothermal amplification buffer ...
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bioRxiv - Neuroscience 2020Quote: ... 1:1000 concentrations of SNAP-Surface 488 (NEB #S9124, New England Biolabs, Ipswich, MA), TMR Halo (G8252 ...
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bioRxiv - Molecular Biology 2020Quote: ... digested overnight at 50°C with 1 μl 20 U/μl SfiI (NEB R0123S) in 200 μl 1x CutSmart buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... digested overnight at 50°C with 1 μl 20 U/μl SfiI (NEB R0123S) then rinsed with 1xTE.
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bioRxiv - Genomics 2022Quote: ... DNA samples were loaded together with Quick-Load 1 kb Extend DNA Ladder (NEB) to aid size determination ...
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bioRxiv - Synthetic Biology 2019Quote: ... 0.25 µl of T4 DNA ligase at 400 U µL−1 (NEB cat. M0202) and 0.25 µl of BsaI (NEB cat ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were stained with DMEM containing 1 µM SNAP-Cell SiR (New England Biolabs) and optionally 0.1 % (v/v ...
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bioRxiv - Microbiology 2019Quote: ... 1 mM calcium chloride and 200 unit/ml final concentrations of micrococcal nuclease (NEB). Cell lysates were incubated at 25 ℃ for 15 minutes before adding 3 mM final concentration of EGTA was added.
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bioRxiv - Biophysics 2019Quote: ... and stained with staining solution (1 µM BG-AF647 [Cat#S9136S, New England Biolabs, Ipswich ...
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bioRxiv - Immunology 2019Quote: ... or SEE together with 1 µM CLIP-Surface 647 (Figure 3C, NEB, catalog S9234S) in RPMI medium for 30 minutes at 37 °C ...
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bioRxiv - Cancer Biology 2019Quote: ... and digested overnight at 37°C with 1 Unit of NlaIII enzyme (R0125L, NEB)/μg of DNA (under agitation-600 rpm) ...
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bioRxiv - Molecular Biology 2019Quote: ... About 1 µg of purified RNA template was transcribed by T7 RNA polymerase (NEB) and the integrity of the RNAs was examined by agarose gel electrophoresis ...
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bioRxiv - Molecular Biology 2019Quote: ... The reannealed fragments were treated with 5 units of T7 endonuclease 1 (NEB, #M0302S) at 37°C for 25 min and immediately separated with 7% polyacrylamide gel electrophoresis.
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bioRxiv - Bioengineering 2019Quote: Phosphorylation master mix (5x): 1 mM Adenosine 5’-Triphosphate (ATP, New England Biolabs, NEB), 0.1 U/µL T4 Polynucleotide Kinase (T4 PNK ...
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bioRxiv - Bioengineering 2019Quote: Phosphorylation master mix (5x): 1 mM Adenosine 5’-Triphosphate (ATP, New England Biolabs, NEB), 0.1 U/µL T4 Polynucleotide Kinase (T4 PNK ...
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bioRxiv - Genomics 2020Quote: ... PCR products were treated with 1 μl of exonuclease (New England Biolabs, 20 units), and the mixture was then incubated at 37°C for 15 min ...
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bioRxiv - Microbiology 2021Quote: ... Denatured RNA was circularised by incubation with 10U T4 RNA Ligase 1 (NEB, USA) in 20 μL of the reaction mixture containing 1x T4 RNA Ligase Buffer (NEB ...
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bioRxiv - Bioengineering 2020Quote: ... PCR 1 conditions: 0.02 U/μL Q5 High-Fidelity DNA Polymerase (New England Biolabs), 1X Q5 reaction buffer ...
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bioRxiv - Neuroscience 2021Quote: Libraries were prepared from 1 ug RNA using a library prep kit (NEB #7770), rRNA depletion kit (NEB #E6310) ...
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bioRxiv - Genomics 2021Quote: ... The template was destroyed by adding 1 μL 20,000 U/mL DpnI (NEB, R0176S) to the mix and incubating at 37 °C for 1 hour ...
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bioRxiv - Cancer Biology 2020Quote: ... and NEBNext Multiplex Oligos for Illumina (NEB Set 1 E7335 and Set 2 E7500S). Additional AMPure clean-ups at the start and the end of library preparation were included ...
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bioRxiv - Neuroscience 2019Quote: ... the reannealed PCR products were digested with T7 endonuclease 1 (T7E1, New England Biolabs). Digestion was performed using 10 unites of T7E1 enzyme and incubated for 15 minutes at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... Annealed primers were then diluted to 1 µl in 200 µl and ligated (NEB) to the digestedand dephosphorylated Cas9 vector.
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bioRxiv - Plant Biology 2021Quote: ... and ligated at a 3:1 amplicon:plasmid ratio with t4 DNA ligase (NEB, US) overnight at 16°C ...
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bioRxiv - Microbiology 2021Quote: ... RNA was then removed by incubation with 1 µl of RNase H (NEB, USA) for 20 minutes at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... then directly resuspended in 1× SDS (sodium dodecyl sulphate) sample buffer (New England BioLabs) per 1 ml of OD600 of 1.0 cells in 200 μl sample buffer ...