Labshake search
Citations for New England Biolabs :
1551 - 1600 of 3576 citations for Vanin 1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2024Quote: ... 1 µl (500 Units) of PNGase F (New England BioLabs, Inc.) was used to deglycosylate 10 µg of the protein in the presence of 5 mM DTT and mild detergents by incubating at room temperature for 1 hour according to manufacturer’s recommendations.
-
bioRxiv - Microbiology 2024Quote: ... and 1 μL of terminal transferase (20 units, NEB Catalog # M0315S) was added to the reaction with incubation at 37 °C for 1 h to block any pre-existing strand-break sites ...
-
bioRxiv - Genetics 2023Quote: ... We added phasing nucleotides as well as overhangs for indexing primers using primer mixtures SL5.F[1-4] and SL5.R[1-4] (NEB Q5 for 20 cycles, Tm 62 °C). We finally added dual indexing primers using the i5 and i7 system from Illumina (NEB Q5 for 20 cycles ...
-
bioRxiv - Molecular Biology 2019Quote: ... we supplemented the reaction with 1 U micrococcal nuclease (New England Biolabs) and allowed it to incubate for 10 min ...
-
bioRxiv - Genetics 2021Quote: ... 1 ul of 20 μM Cas protein (SpyCas9 and SauCas9 from NEB; Nme2Cas9 was purified as previously described (Edraki et al ...
-
bioRxiv - Genetics 2021Quote: ... and the sample was digested with DNase I (NEB [1:20 dilution]) for 30 seconds at room temperature ...
-
bioRxiv - Developmental Biology 2020Quote: ... and 30 μg was diluted 1:3 in gel loading buffer (NEB), sonicated ...
-
bioRxiv - Molecular Biology 2020Quote: ... followed by adding 1 μL of proteinase K (800 units/ml) (NEB) and incubating at room temperature for 10 minutes before being run on a 1.7% agarose gel with a 100 bp ladder for 35 minutes.
-
bioRxiv - Molecular Biology 2021Quote: ... and together with the addition of apyrase (25 mU mL−1, NEB). No CID2886111 was supplied in the purification ...
-
bioRxiv - Molecular Biology 2021Quote: ... and 1 μl ProtoScript II RT (200 U/μl, New England Biolabs) to a total volume of 20 μl ...
-
bioRxiv - Molecular Biology 2021Quote: ... 1 U Q5 hot start high fidelity DNA polymerase (New England Biolabs), 500 nM each primer (Table 2 primers #1-4 for 1116NS19.9 or primers #5-8 for 5b1 ...
-
bioRxiv - Molecular Biology 2020Quote: ... 70 μl of end-repair mix was added (1× Ligation buffer (NEB), 357 μM dNTPs ...
-
bioRxiv - Genomics 2020Quote: ... Chromatin was digested in situ using restriction enzyme CviKI-1 (NEB, R0710L) and barcoded using RELACS custom barcodes (4bp UMI + 8bp RELACS barcode ...
-
bioRxiv - Pathology 2019Quote: ... 1 µl of either SP6 or T7 polymerase (New England Biolabs, USA). SP6 reactions were incubated at 40°C for 2 hours ...
-
bioRxiv - Molecular Biology 2019Quote: ... and 1 μL of Index (X) Primer (25 mmol) (NEB, Ipswich, USA). The reaction conditions were as follows ...
-
bioRxiv - Pathology 2019Quote: ... 1 μL (2 units) DNAse I (M0303S, New England BioLabs, Ipswich, MA), and 89 μL nuclease-free H2O ...
-
bioRxiv - Cancer Biology 2019Quote: ... and the cell pellets were resuspended in 345μl of 1× NEBuffer2 (NEB) per 5 million cell aliquot ...
-
bioRxiv - Microbiology 2020Quote: ... 0.8 mg ml-1 bovine serum albumin (BSA, New England Biolabs GmbH), 0.2 mM of each dNTP (Bioline ...
-
bioRxiv - Developmental Biology 2020Quote: ... digested vectors by a 1:2 mixture of T4 ligase (NEB, M0202L) and T4 polynucleotide kinase (NEB ...
-
bioRxiv - Microbiology 2019Quote: ... T5 exonuclease diluted 1:5 with 5X reaction buffer (New England BioLabs) (0.01 units/µL) ...
-
bioRxiv - Cell Biology 2019Quote: ... 1 μL DNA Pol I and 20 U DNase I (NEB, U.K.), 1 mM each of dATP ...
-
bioRxiv - Molecular Biology 2019Quote: ... Oligonucleotides (1) and (4) were phosphorylated with T4 PNK (New England Biolabs) to allow ligation to the backbone fragment ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... and anti-Phospho-histone H3 1:100 (New England Biolabs, Ipswich, USA) and secondary antibodies AlexaFluorTM 488 goat anti-chicken and AlexaFluorTM 594 goat anti-rabbit IgG (Life Technologies ...
-
bioRxiv - Genomics 2021Quote: ... augments Cap-specific 5’ adapter ligation by T4 RNA ligase 1(NEB). The 3’ adapter was ligated using truncated T4 RNA ligase 2 (NEB ...
-
bioRxiv - Cancer Biology 2021Quote: ... The membrane was incubated with HRP-conjugated secondary antibodies (1:5000, NEB) for 45 mins at RT ...
-
bioRxiv - Molecular Biology 2021Quote: ... and 1 μl of T4 RNA ligase I (10U final, NEB M0437M) were combined in 20 μl total volume and incubated for 16 h at 16 °C ...
-
bioRxiv - Synthetic Biology 2021Quote: ... 1 µL of 10mM dNTPmix and 2 µL of Phusion polymerase (NEB). PCR products were purified using the QIAquick PCR purification column and eluted with 30 µL Qiagen Elution Buffer ...
-
bioRxiv - Microbiology 2021Quote: ... 1 μl of 10-mM deoxynucleoside triphosphate mix (NEB, Ipswich, Massachusetts, USA), 1 μl of each 25-μM primer ...
-
bioRxiv - Developmental Biology 2021Quote: ... rabbit anti-SNAP (1:1000, polyclonal, New England Biolabs, Ipswich, MA, P9310S), or mouse anti-tubulin (1:40,000 ...
-
bioRxiv - Biochemistry 2021Quote: MtDNA was treated with 1 μl uracil-DNA glycosylase (UDG) (NEB, M0280), 1.5 μl Endonuclease IV (NEB ...
-
bioRxiv - Cell Biology 2021Quote: ... overnight using 40 units of T4 RNA ligase 1 (New England Biolabs) in PNK buffer with RNase inhibitors at 16°C ...
-
bioRxiv - Systems Biology 2021Quote: ... Beads were resuspended at 5 µg µl-1 followed by MseI (NEB) digestion according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... 1 µg RNA was reverse transcribed using LunaScript RT SuperMix Kit (NEB). qPCR was performed using synthesised cDNA ...
-
bioRxiv - Microbiology 2021Quote: ... T5 exonuclease diluted 1:5 with 5× reaction buffer (New England BioLabs) (0.01 units/μl) ...
-
bioRxiv - Microbiology 2021Quote: ... 1 mM CTP and 0.6 μl T7 polymerase mix (New England Biolabs.) This target mix was then loaded on to the chip by placing 10 μL of the mix on the sticky side of the PCR film (MicroAmp ...
-
bioRxiv - Cell Biology 2020Quote: ... as well as the 1 kb extended markers (New England BioLabs, #N3239) were used ...
-
bioRxiv - Microbiology 2022Quote: ... and 1 U of OneTaq polymerase (New England BioLabs Inc., Ipswich, MA) in a final volume of 25 μl ...
-
bioRxiv - Systems Biology 2022Quote: ... or Quick-Load® 1 kb Extend DNA Ladder (New England Biolabs) was used for the reference of DNA length ...
-
bioRxiv - Biochemistry 2022Quote: ... The hybrid was reacted with 1 μl RNase H (New England BioLabs) at 37 °C for 30 min and then heated to 90 °C for 10 min to terminate the reaction ...
-
bioRxiv - Genomics 2022Quote: ... 500 nL of 5hmC-blocking mix (1 U T4-BGT (NEB, M0357L), 6x UDP-glucose ...
-
bioRxiv - Neuroscience 2022Quote: ... augments Cap-specific 5’ adapter ligation by T4 RNA ligase 1 (NEB)(Hetzel et al. ...
-
bioRxiv - Plant Biology 2021Quote: ... 1 μL T4 RNA Ligase Buffer (New England BioLabs; Ipswitch, MA, USA), and 40 units RNaseOUT (Invitrogen ...
-
bioRxiv - Plant Biology 2021Quote: ... 10 units T4 RNA Ligase 1 (New England BioLabs; Ipswitch, MA, USA), 1 μL T4 RNA Ligase Buffer (New England BioLabs ...
-
bioRxiv - Microbiology 2021Quote: ... 1×T4 DNA Ligase Buffer and 800 U T4 DNA ligase (NEB). Then heat inactivation was performed for BcgI at 65 °C for 20 min.
-
bioRxiv - Molecular Biology 2020Quote: ... and resuspended in 46.5 μl of 1× Quick ligation buffer (NEB, M2200). 2.5 μl of DNA Quick ligase (NEB ...
-
bioRxiv - Microbiology 2021Quote: ... T5 exonuclease diluted 1:5 with 5X reaction buffer (New England BioLabs) (0.01 units/μL) ...
-
bioRxiv - Cell Biology 2021Quote: ... clarified lysates were treated for 1 h with λPP (New England BioLabs) as described above ...
-
bioRxiv - Cell Biology 2021Quote: ... Clarified lysates were treated for 1 h with λPP (New England BioLabs) as described above ...
-
bioRxiv - Cell Biology 2021Quote: ... and incubated with 1% Bovine Serum Albumin (BSA, New England Biolabs, B9000S) in PBST for 1 hour at room temperature ...
-
bioRxiv - Microbiology 2021Quote: ... 7 μl PEG8000 and 1 μl T4 RNA ligase 2 K227Q (NEB) at 25 °C for 1 h ...