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Citations for New England Biolabs :
2001 - 2050 of 3576 citations for Vanin 1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... using a 5-fold excess of fluorophore to protein and ∼1 μM Sfp synthase (NEB) for 16 hours at 6 °C in 50 mM TRIS-HCl pH 7.5 ...
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bioRxiv - Genetics 2019Quote: ... Using the 100 bp and 1 kb DNA Quick-Load ladders (N0467 and N0468, NEB) as a reference ...
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bioRxiv - Biochemistry 2020Quote: ... pre-tRNA stock was diluted 1 in 2 into RNA loading buffer (New England Biolabs) and separated on a 10% acrylamide urea-TBE denaturing gel ...
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bioRxiv - Genetics 2021Quote: ... 1 μl of 10 mM dNTPs and 0.5 μl of OneTaq Polymerase (New England BioLabs). To generate (partially ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 1 μL cDNA was used in a 30-cycle PCR using Q5 DNA Polymerase (NEB) with primer 8 & 9 or 10 & 11 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 1 μL cDNA was used in a 30-cycle PCR using Taq DNA Polymerase (NEB) with primer 4 & 6 or 5 & 6 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Purified products were ligated with 1 µL T4 DNA ligase (protocol as per NEB®) for 10 minutes at room temperature (benchtop ...
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bioRxiv - Bioengineering 2021Quote: ... for 16 cycles annealing at 67 °C was mixed with 1 μL of ExoI (NEB) digested mixture as the template ...
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bioRxiv - Biochemistry 2021Quote: ... for 1 hr at 37 °C and Proteinase K (4 U; P8107S, NEB, Ipswich, MA) for another 2 hr at 55 °C ...
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bioRxiv - Biochemistry 2021Quote: ... 5 μg standard glycoprotein was mixed in 1 μL of 10× glycoprotein denaturing buffer (NEB) (final conc ...
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bioRxiv - Biochemistry 2020Quote: ... 5:1 molar ratio of insert to backbone and 1X Gibson Assembly Master Mix (NEB) and incubated at 50°C for 1 hour before transformation into One Shot TOP10 Chemically Competent E ...
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bioRxiv - Biochemistry 2020Quote: ... 5:1 molar ratio of insert to backbone and 1X Gibson Assembly Master Mix (NEB) and incubated at 50°C for 1 hour before transformation into One Shot TOP10 Chemically Competent E ...
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bioRxiv - Plant Biology 2021Quote: ... total or virion DNA was treated with 1 unit of Mung Bean nuclease (NEB, Massachusetts). The digestion products were analyzed for SEGS-2 episomes as described above.
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bioRxiv - Microbiology 2020Quote: ... T5 exonuclease diluted 1:5 with 5X reaction buffer (New England BioLabs; 0.01 units/μL), Taq DNA ligase (New England BioLabs ...
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bioRxiv - Genetics 2021Quote: ... 250 ng – 1 μg of RNA samples were digested with Nucleoside Digestion mix (NEB, M069S) for 2 hr at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... PBT was removed and SNAP substrate diluted in PBT (SNAP-Surface649, NEB S9159S; 1:1000) added ...
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bioRxiv - Genomics 2021Quote: ... into 96-well plate containing 2.5μl of methylase reaction buffer (1 × M.CviPI Reaction buffer (NEB), 2 U M.CviPI (NEB) ...
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bioRxiv - Cell Biology 2021Quote: ... and 70 μl water and 1mM MnCl2 added together with 1 μl DNaseI (NEB #M0303S) for 10 minutes at room temperature in order to degrade any DNA that precipitated overnight ...
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bioRxiv - Developmental Biology 2022Quote: ... total RNA (1 µg) extracted from adult mouse testes was treated with alkaline phosphatase (NEB), de-phosphorylated ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were recovered in 1 ml NEB® 10-beta/Stable Outgrowth Medium (NEB, B9035) at 37 °C for 1 hour ...
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bioRxiv - Genetics 2022Quote: ... The supernatant (~12 ml) was added to 1 ml HEGX washed chitin magnetic resin (NEB) and left to bind for 1 h at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... the library was treated with a 1:20 dilution of USER enzyme (New England Biolabs) at 37 °C for 30 min ...
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bioRxiv - Developmental Biology 2021Quote: ... at least 1×106 fixed cells were digested using the DpnII restriction enzyme (NEB, R0543M). Chromatin was re-ligated with T4 ligase (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... with ISPCR (IDT; 0.1 μM) and Lambda Exo (New England BioLabs MO262S; 1 units/reaction) for 12 or 14 total cycles.
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bioRxiv - Microbiology 2021Quote: ... we treated the purified RT reaction with 1 μl of Exonuclease I (NEB, Catalog# M0293L) at 37°C for 30 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... (2019): 1) the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, E7760) was used for library preparation ...
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bioRxiv - Genomics 2022Quote: ... Each “PCR#1” reaction contained 25 µL of Q5 High-Fidelity 2X Master Mix (NEB), 2.5 µL of Nuc PCR#1 Fwd Primer (10 µM) ...
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bioRxiv - Biochemistry 2022Quote: ... 5 μl of 2x Quick Ligase buffer and 1 μl of Quick ligase (NEB, M2200) in a final volume of 10 μl was incubated for 10 min at RT and then used to transform DH5α competent cells ...
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bioRxiv - Biochemistry 2019Quote: ... per 1µg protein for 1 hour at 30°C in PMP buffer (New England Biolabs) plus 1mM MnCl2 ...
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bioRxiv - Microbiology 2019Quote: ... The plasmid was cut and de-phosphorylated simultaneously using 1 U Shrimp Alkaline Phosphatase (NEB) and 20 U EcoRV-HF (NEB ...
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bioRxiv - Genomics 2019Quote: ... mRNAs were isolated from 1 μg of total RNA using a PolyA selection kit (NEB) and sequencing libraries were prepared using the NEBnext Ultra RNA library prep kit for Illumina (NEB) ...
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bioRxiv - Genetics 2019Quote: ... mRNAs were isolated from 1 μg of total RNA using a PolyA selection kit (NEB) and sequencing libraries were prepared following instructions from NEBs Ultra Library Preparation Kit for Illumina ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 μg total RNA was depleted of rRNA using the NEBNext rRNA Depletion kit (NEB). To capture nascent RNA ...
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bioRxiv - Molecular Biology 2020Quote: ... each with 1 μg of gDNA first being digested with 4 U of MmeI (NEB) for 2 hours at 37 °C ...
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bioRxiv - Synthetic Biology 2019Quote: ... 5 μl of the resulting amplicon were diluted 1:4 in 1x buffer 2 (NEB), followed by denaturation and re-annealing in a nexus GSX1 Mastercycler (Eppendorf ...
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bioRxiv - Microbiology 2020Quote: ... and 1 μL of 200 U M-MuLV reverse transcriptase enzyme (B0253S, New England Biolabs). Negative controls were reactions without the RT enzyme and without sample ...
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bioRxiv - Microbiology 2021Quote: ... Protein purification was done in eight steps: (1) amylose resin high flow (New England Biolabs) chromatography using a step elution from 0 mM to 10 mM maltose in Tris buffer A (20 mM Tris/HCl ...
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bioRxiv - Molecular Biology 2020Quote: ... and treated amplicons were visualized on ~1% agarose gels with 100 bp DNA ladder (NEB) and SYBR Gold (Thermo Fisher Scientific).
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bioRxiv - Biophysics 2021Quote: ... 1 mM DTT at pH 7.9) containing 30 units/ml of T4 DNA polymerase (NEB), 100μg/ml single stranded DNA binding protein (gp32 ...
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bioRxiv - Genomics 2021Quote: ... 1 μl of RNAse inhibitor and 3 μl of Antarctic phosphatase (New England BioLabs Inc.). We then performed a kinase treatment adding 4 μl of T4 Polynucleotide Kinase (PNK ...
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bioRxiv - Microbiology 2021Quote: ... 0.7 μL of 20 mg mL-1 bovine serum albumin (New England Biolabs, MA, USA), 0.75 μL of each primer (10 μM) ...
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bioRxiv - Immunology 2020Quote: DNA encoding SARS-CoV-1 VH regions was amplified by PCR (using Q5 polymerase NEB). J segments were modified as required to match the following protein sequence (GTLVTVSS) ...
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bioRxiv - Molecular Biology 2020Quote: ... Reactions were undertaken in 25 ul consisting of 1 x Long amp Taq buffer (NEB), 300 μM dNTP mix ...
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bioRxiv - Molecular Biology 2021Quote: ... The beads were mixed with 1× SDS–PAGE sample loading buffer (New England Biolabs # B7703S) and incubated at 98°C for 5 min ...
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bioRxiv - Neuroscience 2021Quote: We next linearized the vector pscAAV-Hsp68-EGFP (Supplemental Figure 1) using PacI (NEB #R0547L) and AscI (NEB #R0558L) ...
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bioRxiv - Cancer Biology 2021Quote: ... for 1 h followed by incubating with 2 μL Proteinase K (New England Biolabs, USA) at 65 °C ...
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bioRxiv - Developmental Biology 2021Quote: A working lysis buffer solution was prepared by adding 1 µL of the MNase (NEB) [1:50 dilution] per 5 µL lysis buffer ...
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bioRxiv - Microbiology 2020Quote: ... 30 mM acetyl phosphate or 1 µl Calf Intestinal Alkaline Phosphatase (CIP) (NEB, catalog#: M0290V) was added to the solution ...
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bioRxiv - Genomics 2021Quote: ... The 3′ adapter (sequence: AGATCG-GAAGAGCACACGTCTGAACTC) was ligated using T4 RNA Ligase 1 (NEB, M0204L) and purified nascent RNA using streptavidin beads (NEB ...
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bioRxiv - Microbiology 2021Quote: ... and NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1) (NEB #E7600) (New England Biolabs ...