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Citations for New England Biolabs :
1601 - 1650 of 1722 citations for 10 PROPOXY DECANOIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: All purified SNAP-tagged proteins were labeled with 10-fold molar excess of SNAP-Surface dyes (New England Biolabs, Ipswich, MA) in labeling buffer (1× PBS ...
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bioRxiv - Genomics 2024Quote: ... Purified product was Gibson cloned into a previously described PiggyBac transposon vector (37) using standard protocols in a 10 μl reaction (NEB E2621S). 2.5 μl of the Gibson reaction was electroporated into 25 μl of 10-beta electrocompetent E ...
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bioRxiv - Genetics 2023Quote: ... oligonucleotide pools were amplified by 8 to 10 cycles of PCR using NEBNext Ultra II Q5 Master Mix (New England Biolabs, M0544X), digested with BstXI and BlpI ...
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bioRxiv - Biochemistry 2023Quote: ... 0.5 μg of linearized plasmid was used as template in a 10 µL reaction using the HiScribe T7 High Yield RNA Synthesis Kit (NEB # E2040S) with an 8:1 ratio of cap analog to GTP ...
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bioRxiv - Synthetic Biology 2023Quote: ... separation and release of a linear phage DNA from the yeast recombination matrix was performed using 10 µg of in yeast DNA digested using SmaI (NEB, R0141S) for JG024 and ScaI (NEB ...
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bioRxiv - Biophysics 2023Quote: ... we mixed the digested DNA constructs and handles in a 1:10 molar ratio and ligated them together using T4 DNA ligase (M0202, New England Biolabs, UK) at 16°C overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 ng of DNA was used for library preparation according to NEBNext® Ultra II DNA Library Prep Kit (NEB, E7645S). Purity and size distribution of the libraries were estimated using Fragment Analyzer ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 ng of DNA was used for library preparation according to NEBNext® Ultra II DNA Library Prep Kit (NEB, E7645S). Purity and size distribution of the libraries were estimated using Fragment Analyzer ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 ng of DNA was used for library preparation according to NEBNext® Ultra II DNA Library Prep Kit (NEB, E7645S). Purity and size distribution of the libraries were estimated using Fragment Analyzer ...
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bioRxiv - Cancer Biology 2023Quote: ... then incubated for 2 h at 37°C in 100 μl 1x TdT buffer containing 4 μl 10 mM ATP and 1 μl Terminal Transferase (NEB M0315L). Plugs were rinsed with 1 ml Tris buffer (10 mM Tris HCl (pH 8.0)) ...
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bioRxiv - Biochemistry 2023Quote: ... was used to linearize plasmid (10 µg) by incubating at 37 °C for a minimum of 2 hours in 1x CutSmart buffer (NEB, B7204S). Linearized plasmid was purified by extraction with an equal volume of phenol:chloroform:isoamyl alcohol (25:24:1 ...
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Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptomebioRxiv - Microbiology 2023Quote: ... Small RNA libraries were then prepared from 10 ng of small RNA using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs #E7300S) according to the manufacturer’s recommendation using NEBNext Multiplex Oligos for Illumina (Index Primer Set 1 ...
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bioRxiv - Genetics 2023Quote: ... cDNA and sequencing libraries were generated from 500 ng of fresh RNA samples with 10 cycles of PCR with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #7760). After quality checking using an Agilent 2100 Bioanalyzer ...
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Quantitative Comparison of Presenilin Protein Expression Reveals Greater Activity of PS2-γ-SecretasebioRxiv - Cell Biology 2023Quote: ... 10 µM each of sense and antisense synthesised oligonucleotides with appropriate flanking sequences,45 were phosphorylated using T4 PNK (NEB M0201) in a 10 µl reaction as per manufacturer protocol ...
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Quantitative Comparison of Presenilin Protein Expression Reveals Greater Activity of PS2-γ-SecretasebioRxiv - Cell Biology 2023Quote: ... Briefly 2 µM of dsDNA guide was ligated into 10 ng of pSp-Cas9-(BB)-2A-GFP using 400 units T4 DNA ligase (NEB M0202) and incubated for 16 hrs at 16°C ...
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bioRxiv - Microbiology 2023Quote: ... The cDNAs were diluted 1:10 and 2 μl of each used for subsequent PCR reactions with one unit of Taq polymerase (New England Biolabs, MA), 200uM dNTPs (New England Biolabs ...
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bioRxiv - Plant Biology 2023Quote: ... 500 ng of RCA amplicons were treated with T7 endonuclease 5 μL of 10× reaction buffer and 1 μL of T7 endonuclease I (New England Biolabs, M0302S) in a 50 μL reaction volume ...
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bioRxiv - Synthetic Biology 2023Quote: ... The mixture was then returned to ice for 5 minutes before 180 μL of NEB® 10-beta/Stable Outgrowth Medium (B9035, NEB) was added ...
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bioRxiv - Molecular Biology 2023Quote: ... Nascent RNA was then fragmented at 85C for 10 min and converted to cDNA using NEBNext Ultra II RNA First Strand Synthesis Module (NEB, E7771) and NEBNext Ultra II Directional Second Strand Synthesis Kit (NEB ...
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bioRxiv - Genomics 2023Quote: ... The pellet was resuspended in 90 µL of freshly prepared Micro-C “Master Mix 1” (10 μl 10x T4 DNA Ligase Buffer, 75 μl ddH2O, 5 μl T4 PNK (NEB #M0201L)) ...
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bioRxiv - Genomics 2023Quote: Restriction digestion was carried out by adding 25 μL of 10× CutSmart buffer and 100 U of the MluCI restriction enzyme (NEB #R0538) plus 100 U of the MboI enzyme (NEB #R0147) ...
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bioRxiv - Genomics 2023Quote: ... The reaction was diluted to 100 µL with 1X T4 PNK (polynucleotide kinase) buffer and incubated for 30 min with 1 µL T4 PNK (10 U/µL; NEB M0201) to remove 3’ phosphoryl groups ...
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bioRxiv - Molecular Biology 2023Quote: ... Reactions were performed at 37°C for the indicated time (30 min if not otherwise specified) and then terminated by the addition of 10 μL of 2× RNA loading dye (New England Biolabs, USA). Reaction samples were heated at 85°C for 2 min ...
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bioRxiv - Biophysics 2023Quote: ... Trypsin digestion was initiated by adding 1:10 (trypsin to protein, m/m) mass spectrometry grade trypsin (New England Biolabs #P8101S) in 50 mM ammonium bicarbonate ...
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bioRxiv - Molecular Biology 2024Quote: ... 2.5 µg of genomic DNA extracted using the Phenol-Chloroform method and treated with RNase A were digested with 10 units of DpnII (NEB, R0543S) in a final volume of 20 µL at 37°C for 6 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... ligated vectors were then transformed into NEB10-beta electroporation ultracompetent E.coli cells (NEB® 10-beta Electrocompetent E. coli, NEB, Ref.C3020K) and grown overnight on LB plates supplemented with 50 μg/mL Carbenicillin (Carbenicillin disodium salt ...
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bioRxiv - Microbiology 2024Quote: ... 2 μL template cDNA/DNA was used in a 20 μL reaction containing 10 μL Luna® Universal qPCR Master Mix (New England Biolabs). The qPCR program was ...
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bioRxiv - Genomics 2024Quote: ... of 0.1 μM polyT-rootV2 oligo diluted in hybridization buffer (2x SSC, 1 mg/mL yeast RNA, 10% dextran sulfate, 1:1000 Murine RNase inhibitor NEB M0314) on a parafilm sheet placed on a glass plate ...
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bioRxiv - Evolutionary Biology 2024Quote: ... ddRAD library preparation included an initial digestion of 300 ng of DNA in a 34 μL reaction (2 hours at 37 °C; 10 U each of SbfI and MspI, New England Biolabs Inc.). Standard Illumina adapters were ligated using 60 cycles of digestion at 37 °C (2 minutes ...
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bioRxiv - Molecular Biology 2024Quote: dsBC was cloned into the digested vector by combining 200ng vector (∼0.035pmol, 8uL), 10ng dsBC (∼0.175pmol, 2uL) and 10 μL 2x NEBuilder® HiFi DNA Assembly Master Mix (NEB cat#E2621L) and incubating at 50°C for 1hr ...
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Adaptation of CD4 in gorillas and chimpanzees conveyed resistance to simian immunodeficiency virusesbioRxiv - Microbiology 2024Quote: ... Whole-cell extracts were quantified using the BCA assay and 10 µg was subjected to PNGase F (New England Biolabs, #P0705S) treatment according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2022Quote: ... of CROPseq-CaptureSeq-Guide-Puro backbone and 400 fmoles (18.31ng) of amplified dsDNA oligonucleotides were combined with 10 μl of NEBuilder HiFi DNA assembly master mix (NEB cat. no. E2621L) and water to 20 μl ...
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bioRxiv - Physiology 2020Quote: RNA was extracted from cultured cells or tissue (∼10 mg) stored in RNALater® using Monarch® Total RNA Miniprep Kit (New England BioLabs). For maximal RNA recovery ...
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bioRxiv - Genetics 2020Quote: For Southern blot analysis 150 ng of mouse DNA was digested with 10 units of BamHI-HF (NEB, Figure 2 and 5), NdeI (NEB ...
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bioRxiv - Developmental Biology 2019Quote: ... Total RNA was then treated with DNase for 10 min at 37 °C to remove genomic DNA [10 μg RNA in 100 μl reaction buffer with 2U DNase I from NEB (Ipswich, MA)] followed by phenol-chloroform extraction and ethanol precipitation.
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bioRxiv - Genomics 2019Quote: ... PCR reactions for library preparation were performed by adding the following to the 10 μL DNA sample: 25 μL of Phusion High-fidelity PCR Master Mix (NEB catalog # M0531S), 1 μL of 5’ library index barcode ...
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bioRxiv - Plant Biology 2021Quote: ... The F1 fragment of eYGFPuv was assembled by cloning the gblocks and a PCR-amplified relevant fragment into an eYGFPuv vector 10 through NEBuilder HiFi DNA Assembly (New England BioLabs, Catalog #E5520S). Similarly ...
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bioRxiv - Microbiology 2021Quote: ... were cloned into the pCR-Blunt-II vector using the PCR Blunt Topo kit according to the manufacturer’s instructions and transformed into 10-beta CaCl2-chemically competent bacteria (originally NEB, generated in-house). Following amplifications of colonies and purification of plasmids using a QiAprep Mini kit (Qiagen) ...
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bioRxiv - Physiology 2022Quote: ... from epithelium or sensory neurons underwent digestion for 10 hours at 37°C with MspI restriction enzyme (New England Biolabs, Ipswich, MA), followed by purification with AMPure XP (Beckman Coulter ...
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bioRxiv - Molecular Biology 2020Quote: ... and 3 μL of factor mix (with RNA polymerase, and transcription/translation factors in 10 mM Mg2+) from the PURExpress® Δ Ribosome Kit (New England Biolabs). The reaction buffer was based on Shimizu et al ...
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bioRxiv - Microbiology 2021Quote: ... Triplicate NEBuilder HiFi assembly reactions were prepared according to manufacturer’s protocols containing ∼2:1 insert to vector as follows: 10 µl of 2X NEBuilder HiFi master mix (New England Biolabs, cat#E2621S), 158.7 ng of DNA fragments ...
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bioRxiv - Neuroscience 2021Quote: ... Enzyme restriction with DpnII was performed in 30 µl reaction mix (1 µl DpnII, 3 µl DpnII buffer, 10 µl purified DNA and 16 µl H2O; New England Biolabs, Ipswich, MA) and incubation at 37°C for 2h ...
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bioRxiv - Biochemistry 2020Quote: ... α1- 3,4,6), 10 U β-galactosidase (P0726S, β1-3) and 8 U β-galactosidase (P0746S, β1-3,4) (all from New England BioLabs, USA). All reactions were performed in a final volume of 10 μl in 50 mM sodium acetate buffer ...
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bioRxiv - Biophysics 2021Quote: ... The surface is then passivated by incubating bovine serum albumin (BSA, New England Biolabs, 10 mg/ml in PBS and 50% glycerol) for 30 minutes ...
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bioRxiv - Cell Biology 2019Quote: ... by random priming without denaturation with 4 mg random nonamer oligo (Integrated DNA Technologies, Skoie IL) and 10 units of Klenow (New England Biolabs, Ipswich, MA). Unincorporated dye was removed with Microcon columns (30 kDa MW cutoff ...
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bioRxiv - Synthetic Biology 2020Quote: ... were PCR amplified from genomic DNA of yeast strain BY474129 and cloned together with the bidirectional inducible Gal1-10 promoter into the PCR amplified pRSII42B backbone with OZL14 and OZL15 via Gibson assembly30 using NEBuilder Kit (NEB, catalog E2621). MMR-DN mutant genes were subsequently generated via site-directed mutagenesis using either Q5 Site-Directed Mutagenesis Kit (NEB ...
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bioRxiv - Genomics 2020Quote: ... and 10-12 day adult TRAP libraries were prepared with the NEBNext Ultra RNA library Prep Kit for Illumina (NEB, product E7530S). ChIP-seq libraries were prepared using the NEBNext ChIP-seq Library Prep Master Mix Set for Illumina (NEB ...
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bioRxiv - Plant Biology 2020Quote: ... The suitability of the selected restriction enzyme pair was confirmed by digesting 400 ng of genomic DNA using 5 Units of each restriction enzyme and NEB CutSmart™ buffer (10×) (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Cancer Biology 2020Quote: ... Up to 10 ng of cDNA was used for the Illumina sequencing library construction using NEBNext® Ultra™ DNA Library Prep Kit (NEB). Paired ends sequencing was performed on NextSeq 500 (Illumina ...
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bioRxiv - Plant Biology 2020Quote: ... and the quality was checked by digestion of 250 ng of genomic DNA from 10 random samples with the restriction enzyme EcoRI (New England Biolabs, Boston, MA) at 65°C for 2 hour and thereafter visualised on agarose gel.