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Citations for New England Biolabs :
1551 - 1600 of 1722 citations for 10 PROPOXY DECANOIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... 20µg of protein lysates and media samples were incubated with glycoprotein denaturation buffer at 95°C for 10 min and treated with 0.3µl Endo H (NEB; Catalog no #P0702) enzyme in glyco-buffer 3 for one hour at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Spacers were generated by annealing and phosphorylating the two oligos (PSP116 and PSP117 for GFPT; PSP120 and PSP121 for NT40) at 10 µM each in T4 ligation buffer (NEB B0202S) with T4 polynucleotide kinase (NEB M0201S ...
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bioRxiv - Molecular Biology 2020Quote: ... then incubated for 2 h at 37°C in 100 μl 1x TdT buffer containing 4 μl 10 mM ATP and 2 μl Terminal Transferase (NEB M0315L). Plugs were rinsed with 1 ml tris buffer (10 mM Tris HCl pH 8.0) ...
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bioRxiv - Bioengineering 2021Quote: ... MWCO 10,000 filters and then digested with 10 µl Tobacco etch virus nuclear-inclusion-a endopeptidase (TEV) (New England Biolabs, Ipswich, MA) overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... then the single-stranded oligos (1 ng) were PCR amplified for 10 cycles with Q5 High-Fidelity DNA Polymerase (NEB, M0491L) using Oligo_Amp_F and Oligo_Amp_R primers to generate double-stranded DNAs ...
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bioRxiv - Synthetic Biology 2022Quote: ... and eluted in 10 μL of Ultra Pure™ DNase/RNase free distilled water: the purified amplicon and the 1kb DNA ladder (NEB) were run on 1% agarose gel in 1x TBE for 1 h 30 min at 110 V ...
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bioRxiv - Microbiology 2019Quote: Bacterial 16S rRNA genes were amplified from 50 ng DNA per sample diluted to 10 µL nuclease free water in 50 µL with 1 × LongAmp Taq master mix (New England Biolabs, Inc), 1 µL 16S rRNA gene barcoded primer (ONT-SQK-RAB-201 ...
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bioRxiv - Microbiology 2020Quote: ... 2 μg of the extracted genomic DNA was digested for 4 h at 37☐ using 10 U MmeI (New England Biolabs). The DNA was then immediately dephosphorylated by treatment with 1 U calf intestine alkaline phosphatase (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... We prepared a library with a fragment size of 300 nucleotides and used the 10 μM NEBNext Multiplex Oligos for Illumina (Set 1, NEB #E7335) lot 0091412 and the NEBNext multiplex Oligos for Illumina (Set 2 ...
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bioRxiv - Systems Biology 2019Quote: ... the Nickel-NTA beads were incubated in 80 μl 3’-linker ligation mix with (1 X PNK buffer, 1 µM 3’-adapter, 10% PEG8000, 30U Truncated T4 RNA ligase 2 K227Q (NEB, M0351L), 60U RNasin) ...
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bioRxiv - Plant Biology 2020Quote: ... The suitability of the selected restriction enzyme pair was confirmed by digesting 400 ng of genomic DNA using 5 Units of each restriction enzyme and NEB CutSmart™ buffer (10×) (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Cell Biology 2020Quote: Glands were dissected in cold PB+RVC (100 mM Na Phosphate Buffer pH 7.2, 10 mM Ribonucleoside-vanadyl complex; New England Biolabs, Ipswich, MA). During dissection ...
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bioRxiv - Bioengineering 2019Quote: ... eukaryotic recombinant Stlac2 was denatured with Glycoprotein denaturing buffer at 100 °C for 10 min to be deglycosylated using PNGase F (NEB, USA) according to instructions ...
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bioRxiv - Genetics 2020Quote: ... We then eluted the DNA from the beads using 10 μl of water and added to it 25 μl NEBNext HiFi 2x PCR MasterMix (NEB M0541), with 2.5 uL of each of the dual-indexed Illumina Nextera primers (25 μM) ...
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bioRxiv - Cell Biology 2021Quote: ... The 5’ RNA linker (5’-blocked and containing a 10 nt UMI (/5AmMC6/GUUCAGAGUUCUACAGUCCGACGAUCNNNNNNNNNN3’) was ligated to the RNA fragments on bead using T4 RNA ligase (NEB M0437) for 2 h at 24°C with shaking ...
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bioRxiv - Synthetic Biology 2021Quote: ... Vector and miRNA target inserts were ligated at 1:10 molar ratio using 400 U of T4 DNA Ligase (NEB, M0202) using provided Ligase buffer in a reaction volume of 20 µL over night at 16 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 μl of RNA were mixed with 2 μl of 100 μM PvG748-SBS12-RT random hexamer primer (Supplementary Table 3) and 1 μl of 10 mM dNTP mix (NEB, N0447S), incubated for 3 minutes at 65 °C and transferred to ice ...
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bioRxiv - Genomics 2021Quote: ... and supernatant was transferred to a new tube and added to 2 μL 10% SDS and 2 μL 20 mg/mL proteinase K (New England Biolabs P8107). Samples were incubated overnight at 65°C to reverse crosslinks ...
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bioRxiv - Genomics 2022Quote: ... Primers which had then annealed in adjacent positions were ligated through the addition of 10 U (20 μL) Taq ligase (NEB M0208L) and incubation at 55°C for 1 hour then 75°C for 10 min ...
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bioRxiv - Genomics 2019Quote: ... We tested nuclear DNA removal by starting with 1 µg of total DNA using either 10 or 20 Units of Exonuclease V (New England Biolabs; UK) and an incubation of 30 minutes or 2 hours at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 µL of a 1000 U of DNase I stock was added to 500 µL of DNase I buffer on cells for 10 minutes or 2 µL of a 5000 U of RNase H stock (NEB M0297S) was added to 500 µL of RNase H buffer on cells for 5 minutes at 45 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... and sequencing adaptors (5 μl) from the Ligation Sequencing Kit (ONT, #LSK109) and Quick T4 DNA Ligase (10 μl) (NEB, M2200S) were added to the cleaved and dA-tailed gDNA sample ...
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bioRxiv - Microbiology 2021Quote: ... 5 μl of 10 mM MnCl2 and 1-2 μl (400-800 units) of λ PP (New England Biolabs, Ipswich, MA). Untreated lysates received 1-2 μl of water in place of λ PP ...
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bioRxiv - Immunology 2021Quote: ... 10 µL diluted cDNA was used with the NEB Luna® Universal Probe qPCR Master Mix (New England BioLabs® Inc.) following the manufacturer’s protocol and performed in 384-well plates using a QuantStudio™ 5 Real-Time PCR System (Applied Biosystems™) ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μl 10 pM/μl TrAEL-seq adaptor 1 (49) and 1 μl T4 RNA ligase 2 truncated KQ (NEB M0373L). Plugs were then rinsed with 1 ml tris buffer ...
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bioRxiv - Microbiology 2022Quote: ... 2 µL of 10 mM indexed P5 and P7 primer solutions and 25 µL NEBnext High-Fidelity 2X Master Mix (New England BioLabs: ME541L) were added ...
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bioRxiv - Biophysics 2022Quote: ... the reaction was diluted to 90 µl and was supplemented with 10 µl DNAse I buffer and 2 µl DNAse I enzyme (NEB #M0303S) and incubated for 15 minutes at 37□ C to degrade the DNA template ...
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bioRxiv - Bioengineering 2022Quote: ... 1X of a restriction enzyme mix [1/2 EcoRI-HF® (NEB #R3101, 2/5 Nuclease-free water, 1/10 CutSmart Buffer 10X (NEB)] ...
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bioRxiv - Genomics 2022Quote: ... 10 U of the enzyme was used in the 50 μl-reaction containing 5 μl of rCutSmart Buffer (10X, NEB # B6004S) for 30 min-incubation at 37°C ...
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bioRxiv - Genomics 2022Quote: ... from ONTs ligation sequencing kit (ONT, SQK-LSK109) and 10 µl of NEBNext Quick T4 DNA Ligase (New England Biolabs, E6056S). Ligation mix was incubated at RT for 30 min ...
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bioRxiv - Microbiology 2022Quote: ... was used as a template to prepare 10 μl cDNA using LunaScript®RT SuperMix Kit (New England Biolabs, cat# E3010L) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Purified RNA was resuspended in 10 µM VRA3 adaptor (/5Phos/rGrArUrCrGrUrCrGrGrArCrUrGrUrArGrArArCrUrCrUrGrArArC/3InvdT/) and treated with T4 RNA Ligase I (New England Biolabs M0437) for 2 h at room temperature (22°C ...
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bioRxiv - Microbiology 2023Quote: ... The sonicated DNA was end-repaired for 30 minutes at 20°C in 200 μl final volume containing 1x end-repair buffer and 10 μl of end-repair mix (NEB, E6050L). The reaction was cleaned in 2x AmpureXP Beads (Beckman Coulter ...
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bioRxiv - Genomics 2022Quote: ... PCR amplification was carried out by addition of 5 µL 10 µM Nextera index mix(Vazyme, #TD203) and 25 µL Q5 High-Fidelity 2X master mix (NEB, #M0492S) to the 20 µL sample ...
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bioRxiv - Neuroscience 2023Quote: ... RNA-seq libraries were prepared with 10 ng of total RNA using NEBNext® single cell/low input RNA library preparation Kit (New England Biolabs). Single-end sequencing was performed on all the samples with a 75 bp sequencing depth on a NextSeq machine at the RNomics platform of the Université de Sherbrooke (Canada) ...
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bioRxiv - Molecular Biology 2022Quote: ... Up to 10 ng of DNA was used for the library construction using NEBNext Ultra II DNA Library Prep Kit (NEB, E7645). Sequencing was performed on NextSeq 500 (Illumina).
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bioRxiv - Genomics 2022Quote: We constructed NEBNext libraries with 200- and 10-ng RNA by following the guideline of NEBNext Ultra II RNA Library Prep Kit for Illumina kit (NEB, E7770). SmartSeq2 libraries with single cells were prepared following the protocol that was reported by Picelli ...
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bioRxiv - Cell Biology 2022Quote: ... Recombinant cohesinWT or cohesin3D (25 nM) were incubated with 50 nM NIPBL-MAU2 and 10 ng/μl λ-DNA (NEB, N3011S) in ATP reaction buffer consisting of 20 mM NaH2PO4/Na2HPO4 pH 7.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... an optional PBS wash followed by Red Blood Cell lysis step was included (10 mins at RT) prior to hypotonic lysis with Nuclei Prep Buffer (NEB, Cat.T3052) and Rnase digestion ...
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bioRxiv - Molecular Biology 2024Quote: ... 2.5 uL each 10 uM forward and reverse primers (cTF256, cTF257) and 25 uL NEBNext Ultra II Q5 Master Mix (NEB, M0544) and ran the following thermocycling protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... 2.5 uL each 10 uM forward and reverse primers (cTF254, cTF255) and 25 uL NEBNext Ultra II Q5 Master Mix (NEB, M0544) and ran the following thermocycling protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... 2.5 uL each 10 uM forward and reverse primers (cTF223, cTF218 - see Supp. Table 2) and 25 uL NEBNext Q5U Master Mix (NEB, M0597) and ran the following thermocycling protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... 4 μg of venom in 10 µL of reducing SDS-PAGE buffer (6X stock solution, NEB B7024S with 30% β-mercaptoethanol) was run per lane on BioRad™ Mini PROTEAN pre-cast TGX acrylamide gels (15 well ...
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bioRxiv - Microbiology 2024Quote: ... RT-qPCR reactions were carried out in 10 μL reactions using the Luna® One-Step Universal RT-qPCR kit (NEB) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... 250 pmol of each RNA oligo was treated with 10 units calf intestinal alkaline phosphatase (CIP, New England Biolabs Inc., M0290S) in 37°C for 30 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... The plugs were washed in 20 ml of Milli-Q water for 30 minutes and then 10 ml of 1x rCutSmart buffer (New England Biolabs, B6004S) for 1 hour followed by a further hour in 5 ml of 1x rCutSmart buffer ...
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bioRxiv - Genomics 2024Quote: ... Purified product was Gibson cloned into a previously described PiggyBac transposon vector (37) using standard protocols in a 10 μl reaction (NEB E2621S). 2.5 μl of the Gibson reaction was electroporated into 25 μl of 10-beta electrocompetent E ...
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bioRxiv - Genetics 2023Quote: ... oligonucleotide pools were amplified by 8 to 10 cycles of PCR using NEBNext Ultra II Q5 Master Mix (New England Biolabs, M0544X), digested with BstXI and BlpI ...
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bioRxiv - Cell Biology 2024Quote: ... Thymidine was then removed by washing cells with culture media and blocking of existing CENP-A was performed by treatment with 10 mM SNAP-Cell® Block BTP (S9106S, NEB) for 30 min ...
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bioRxiv - Cell Biology 2024Quote: All purified SNAP-tagged proteins were labeled with 10-fold molar excess of SNAP-Surface dyes (New England Biolabs, Ipswich, MA) in labeling buffer (1× PBS ...