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Citations for New England Biolabs :
1501 - 1550 of 1722 citations for 10 PROPOXY DECANOIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 1× CutSmart buffer (50 mM Potassium Acetate, 20 mM Tris-acetate, 10 mM Magnesium Acetate, 100 µg/ml BSA or recombinant albumin; NEB), 0.5 mM 1,4-Dithiothreitol (DTT) ...
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bioRxiv - Molecular Biology 2023Quote: ... the remaining 5’-phosphate groups were dephosphorylated using calf-intestinal alkaline phosphatase for 10 minutes at 37°C and finally heat inactivated for 20 min at 80°C (Quick CIP, New England Biolabs). The Rap1 arrays initially cloned in a pUC19-derived vector (Table S2 ...
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bioRxiv - Biophysics 2023Quote: ... The reaction was mix using 1× CutSmart buffer (50 mM potassium acetate, 20 mM Tris-acetate, 10 mM magnesium acetate, and 100 μg/mL BSA; NEB) and 0.5 mM DTT (1,4-dithiothreitol ...
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bioRxiv - Genetics 2023Quote: ... 300 ng of PCR products were digested at 37° C for 1 hour with 10 units of MfeI-HF and SspI-HF in 1X rCutsmart buffer (New England Biolabs, cat ...
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bioRxiv - Bioengineering 2024Quote: ... input genomic DNA was amplified in a 10-μl reaction for 26 cycles using NEBNext High-Fidelity 2x PCR Master Mix (NEB). PCR products were purified using Sera-Mag magnetic beads (Cytiva ...
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bioRxiv - Synthetic Biology 2023Quote: ... 10 ng of the intermediate plasmid products were incubated with 10 uM random hexamer primers and 1X phi29 DNA polymerase buffer (NEB) at 95°C for 3 minutes and then cooled to room temperature ...
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bioRxiv - Immunology 2023Quote: ... Completed libraries were then sequenced to an average depth of approximately 20M reads per sample on a partial lane of the NovaSeq6000 S4 XP flow cell using 2×150 paired-end reads with 10-base dual indexes (CAS: E6440S, NEB). After demultiplexing these samples ...
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bioRxiv - Cell Biology 2023Quote: ... 2 µL i7 unique index primer (10 µM) and 25 µL NEBNext® High-Fidelity 2X PCR Master Mix (NEB) and subjected to the following PCR program ...
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bioRxiv - Biochemistry 2023Quote: ... crosslinking of Pex1-AzF was performed after a 10 min 30 deg C incubation in ATP regeneration mix with and without apyrase (NEB#M0398S ...
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bioRxiv - Genetics 2024Quote: ... a test transformation was performed with 1uL of the 20uL SPRI purified Gibson assembly mixture and 50uL of 10-beta electrocompetent cells (NEB) to determine the optimal amount of Gibson assembly and 10-beta cells to achieve the desired colony-forming unit (CFU ...
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bioRxiv - Genetics 2024Quote: ... preincubated at 37°C for 10 min with either a single-guide RNA (sgRNA) generated by in vitro transcription (SP6 RNA Polymerase, New England BioLabs) or hybridized crRNA/tracrRNA (IDT) ...
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bioRxiv - Genetics 2024Quote: ... a subset of a second identical transformation was taken directly after electroporation and split across ten 1 mL cultures with 10-beta recovery media (NEB) then incubated for 1 hour at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... were performed as per the manufacturer’s instructions using 10 ng/µL of DHFR-Strep reporter plasmid with the addition of 0.8 U/µL RNase Inhibitor Murine (NEB, M0314S). Purified FaRel2-FLAG3 (at 50 nM as final concentration ...
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bioRxiv - Biophysics 2024Quote: ... On-column phosphatase treatment was performed by incubating the resin with 10 mL SEC buffer supplemented with 1 mM MnCl2 and 4,000 units (10 μL) Lambda Protein Phosphatase (LPP) (New England BioLabs P0753L) standing at 4°C overnight ...
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bioRxiv - Cell Biology 2024Quote: ... Each 1 mL aliquot of microsomes was treated for 10 min at 37°C with 4000 U micrococcal nuclease (New England Biolabs), 2 U RNase-free DNase (Promega) ...
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bioRxiv - Genomics 2024Quote: ... Individual libraries for immunoprecipitated DNA and 10% input were dual indexed (NEBNext Multiplex Oligos, unique dual indices, New England Biolabs), PCR amplified and then pooled into equimolar amounts and further quantified using the NEBNext Library Quant Kit (New England Biolabs) ...
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bioRxiv - Microbiology 2024Quote: ... The radioactive probe was prepared from 40 pmoles of primers described in Supplementary Table 7 and labeled at the 5’ end with 10 units of T4 polynucleotide kinase (New England Biolabs) and [γ-32P]-ATP (150 µCi) ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was diluted 1:10 and used for qPCR reactions employing Luna® Universal qPCR Master Mix (New England Biolabs). Ct values were computed by the instrument and used for calculation of ΔΔCt using ACTB (Beta-actin ...
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bioRxiv - Neuroscience 2024Quote: ... 10 ng was used as input into the NEBNext Ultra II DNA Library Prep with Sample Purification Beads (NEB, E7103S). Volumes recommended by the manufacturer were halved ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µl of unpurified RT product was amplified in 10 µl using Q5 High-Fidelity 2X Master Mix (New England Biolabs) with 1 µM of each primer ...
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bioRxiv - Microbiology 2024Quote: ... Samples get 10-fold diluted and procedure was performed using Luna® Universal qPCR Master Mix (New England Biolabs, USA) and rpoD housekeeping gene as reference ...
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bioRxiv - Genomics 2021Quote: ... and washed 3 times with ice-cold 10 mM Ribonucleoside Vanadyl Complex (RVC) (New England BioLabs, cat.no. S1402S, Ipswich, Mass, USA) in buffer A (10 mM NaCl ...
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bioRxiv - Molecular Biology 2019Quote: ... and 10 µl was mixed with 40 µL of 1x NEBuffer 3 supplemented with 10 mM MgCl2 and 5 units of Mbo I (New England BioLabs #R0147L). This reaction was incubated for 1 hour at 37°C ...
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bioRxiv - Genetics 2021Quote: ... We then amplified 10 uL of cDNA in 100 uL PCR reactions using NEBNext Ultra II Q5 Master Mix (NEB, M0544S) and Lib_Seq_eGFP_F2 and Lib_Hand primers for either 8 or 3 cycles (MPRA 1 and 2 respectively) ...
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bioRxiv - Genetics 2021Quote: For CUT&RUN-sequencing libraries were made starting from 10 ng of CUT&RUN DNA fragments using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645S) using the following PCR program ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Adapters for Illumina sequencing were subsequently extended via a 10-cycle PCR with Phusion high-fidelity DNA polymerase (New England Biolabs, Inc.). Final reactions were purified via AMPure XP beads and standardized per submission requirements of the DNA Core Facility (University of Oregon Genomics & Cell Characterization Facility ...
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bioRxiv - Genomics 2020Quote: ... and 0.4 μl of 20 mg/ml BSA with 1 μl (10 U/μl) of T4 endonuclease V (NEB, T4-PDG) and 1 μl (10 U/μl ...
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bioRxiv - Neuroscience 2021Quote: ... Quantitative real-time PCR was performed with 20 ng of cDNA in a total reaction volume of 10 μL using Luna® Universal qPCR Master Mix (Cat. No. M3003L; New England BioLabs) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... The nuclei pellet was resuspended in the Ligation Buffer containing 120 μl 10X NEB T4 DNA Ligase Buffer supplemented with 10 mM ATP (NEB, B0202), 60 μl 20% Triton X-100 (1% final) ...
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bioRxiv - Molecular Biology 2021Quote: ... then incubated for 2 h at 37°C in 100 μl 1x TdT buffer containing 4 μl 10 mM ATP and 1 μl Terminal Transferase (NEB M0315L). Plugs were rinsed with 1 ml tris buffer (10 mM Tris HCl pH 8.0) ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 μl of 10 mM dATP and 3 μl of 5 U/μl of Klenow fragment (3′→ 5′ exo (-)) (NEB, M0212) were added and the sample was incubated for 30 min at 37 °C followed by a deactivation step at 65 °C for 20 min ...
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bioRxiv - Genomics 2022Quote: ... with 0.8μl of HiFi Cas9 Nuclease V3 (62 μM, IDT Cat #1081060) in 10 μl of 10X CutSmart Buffer (NEB, Cat #B7204) at a final volume of 100 μL ...
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bioRxiv - Biochemistry 2020Quote: ... The CIP-treated RNA was then PNK-treated with 1 μL of 10× T4 polynucleotide kinase buffer (New England Biolabs, B0201S), 2 μL of [γ-P32]ATP (PerkinElmer ...
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bioRxiv - Genetics 2019Quote: ... was digested at a concentration of 100 ng / μl overnight in 7 ml in a 15 ml Falcon tube at 37°C with NEB Cutsmart Buffer + 10 units of NotI-HF (New England Biolabs # R3189) per ml ...
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bioRxiv - Genetics 2019Quote: ... coli was performed using DH5-alpha or its commercial derivative NEB 10-Beta (New England Biolabs product number C3019I or C3020). Genomic DNA of BY4741 ...
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bioRxiv - Molecular Biology 2019Quote: ... 948 µL of ligation master mix was then added: 150 µL of 10x NEB T4 DNA ligase buffer with 10 mM ATP (NEB, B0202), 125 µL of 10% Triton X-100 ...
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bioRxiv - Cancer Biology 2019Quote: ... Up to 10 ng of cDNA was used for the Illumina sequencing library construction using NEBNext Ultra DNA Library Prep Kit (NEB, E7645). Paired ends sequencing was performed on NextSeq 500 (Illumina ...
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bioRxiv - Cancer Biology 2019Quote: ... Up to 10 ng of DNA was used for the library construction using NEBNext Ultra II DNA Library Prep Kit (NEB, E7645). Sequencing was performed on NextSeq500 (Illumina ...
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bioRxiv - Synthetic Biology 2019Quote: Fly genomic DNA was isolated in a pool by grinding in 25 µl of “Squish Buffer” (10 mM Tris, 1 mM EDTA, 25 mM NaCl, 8 U/ml ProK (NEB P8107S)) per adult ...
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bioRxiv - Neuroscience 2020Quote: ... 10 μl of the reaction was directly used for restriction digest using 4 units of BssHII enzyme (New England Biolabs, USA) in a 20 μl reaction ...
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bioRxiv - Synthetic Biology 2021Quote: SynHox assemblon BACs were verified by digesting a ∼250-500ng purified by alkaline lysis (72) from small scale (5-10 mL) saturated bacterial culture with PvuI-HF (New England Biolabs R3150S). Digestion reactions were carried out at 37°C for 3-24 hours ...
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bioRxiv - Neuroscience 2020Quote: ... 2 μL P7 primer (10 μM) (5ʹ-CAAGCAGAAGACGGCATACGAG AT[i7] GTCTCGTGGGCTCGG-3ʹ; IDT) and 20 μL NEBNext High-Fidelity 2X PCR Master Mix (NEB, M0541). Amplification was performed using the following program ...
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bioRxiv - Developmental Biology 2021Quote: ... using 200 ng of sheared gDNA and 10 PCR cycles using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645S). The DNA libraries were indexed with unique dual barcodes (8bp long) ...
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bioRxiv - Neuroscience 2020Quote: ... PCR was performed with 10 ng of plasmid DNA template using Q5® Hot Start High-Fidelity 2X Master Mix (New England Biolabs) in a final reaction volume of 25 μL according to the manufacturer’s recommendations except that the extension time was set to 80 seconds per kb for a total of 20 cycles ...
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bioRxiv - Cell Biology 2021Quote: ... and 200 ng of the purified amplicon were end-labeled using 10 µCi of γ-32P ATP (Amersham) and T4 polynucleotide kinase (NEB). The labeling reaction was then desalted on a G-25 spin column and further precipitated with 70% ethanol ...
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bioRxiv - Cell Biology 2021Quote: The recovery probe was prepared as follows: A standard 100 µl PCR reaction was prepared containing 10 ng of bacteriophage λ DNA (NEB), 5 units of Taq polymerase (NEB) ...
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bioRxiv - Biochemistry 2021Quote: ... samples were treated with 1000 U of a form of Endoglycosidase H that does not co-migrate with and hence potentially distort the radiolabelled products when resolved: Endoglycosidase Hf (translation products of ∼10-50 kDa; New England Biolabs, P0703S) or Endoglycosidase H (translation products of ∼50-150 kDa protein substrates ...
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bioRxiv - Genomics 2021Quote: Restriction digestion was carried out by adding 25 μL of 10 ×NEBuffer 2 and 100 U of the MluCI restriction enzyme (NEB, R0538) and incubating for ≥2 hours at 37°C in a Thermomixer at 900 rpm ...
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bioRxiv - Microbiology 2021Quote: ... coli NEB 10-beta via the heat shock method of transformation according to the manufacturer’s instructions (New England Biolabs, Tokyo, Japan). All plasmids were extracted using the QIAprep Spin miniprep kit (QIAGEN ...
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bioRxiv - Biochemistry 2022Quote: ... the used RNA was radioactively labelled on the 5’-end with [γ-32P]ATP (10 mCi/ml, Hartmann Analytic) and T4 PNK (NEB), following purification via PAGE ...