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Citations for New England Biolabs :
1451 - 1500 of 6951 citations for rno mir 155 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... After samples were PCR-amplified using 1×NEBNext High-Fidelity PCR Master Mix (NEB, M0541S). Subsequent libraries were purified with the MinElute PCR Purification Kit (Qiagen ...
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bioRxiv - Physiology 2024Quote: ... After the PCR 20ul of the PCR product were restriction digested with BamH1 (NEB #xxx) for 1 hr at 37C ...
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bioRxiv - Immunology 2024Quote: ... Purified round 2 PCR products were cloned using the NEB PCR Cloning Kit (NEB, #E1202) and sequenced with Sanger sequencing.
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bioRxiv - Molecular Biology 2023Quote: ... The PCR products were purified using Monarch PCR and DNA Cleanup kit (NEB, Ipswich, MA). DNA concentration was quantified using a NanoDrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... First-strand complementary cDNA was synthesized with LunaScript™ RT SuperMix Kits (New England Biolabs). Quantitative PCR was performed with a CFX96 Touch Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Genomics 2020Quote: ... ‘GunIt’ refers to the release (dx.doi.org/10.17504/protocols.io.bdp7i5rn) and ‘LoCost’ includes LunaScript RT SuperMix (NEB) instead of SuperScript IV (Thermo ...
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Efficient Suppression of Endogenous CFTR Nonsense Mutations Using Anticodon Engineered Transfer RNAsbioRxiv - Molecular Biology 2021Quote: ... using the Luna Universal One-Step RT-qPCR Kit (New England BioLabs, #E3005L or #E3006E) according to manufacturer’s instructions and then analyzed with QuantStudio Design & Analysis Software v1.5.1 (Applied Biosystems ...
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bioRxiv - Molecular Biology 2021Quote: 5’ RACE was performed using the Template Switching RT Enzyme Mix (New England Biolabs M0466) according to manufacturer’s instruction ...
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bioRxiv - Bioengineering 2020Quote: The following components comprised the RT-LAMP assay: 4 mM of MgSO4 (New England Biolabs), 1× final concentration of the isothermal amplification buffer (New England Biolabs) ...
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bioRxiv - Genetics 2021Quote: ... RT-qPCR analysis was performed in Luna Universal qPCR Master Mix (New England Biolabs, #M3003) using a Roche LightCycler480 (Roche) ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were run using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) on a CFX384 (Bio-Rad).
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bioRxiv - Neuroscience 2020Quote: RNA was reverse transcribed using LunaScript RT SuperMix Kit (New England Biolabs, Ipswich, MA, USA). To confirm that striatal ChIs or midbrain dopaminergic neurons were collected via LCM ...
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bioRxiv - Biophysics 2021Quote: ... We used the Luna Universal One-step RT-qPCR kit (E3005S, New England Biolabs, MA) to determine the relative abundance of target mRNA between two samples ...
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bioRxiv - Microbiology 2021Quote: ... we treated the purified RT reaction with 1 μl of Exonuclease I (NEB, Catalog# M0293L) at 37°C for 30 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... according to the manufacturer’s instructions and reverse transcribed with the LunaScript RT SuperMix kit (NEB). cDNA was analyzed by quantitative PCR using the CFX96 Touch Real-Time PCR Detection System (BIO-RAD) ...
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bioRxiv - Genetics 2021Quote: ... All RNA samples were reverse transcribed using the LunaScript™ RT supermix kit (#E3010L, NEB), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... using Luna Universal Probe One-Step RT-qPCR Kit (New England BioLabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... 5 μl RT mastermix (2x reaction buffer, 20 mM DTT, 4U murine RNase inhibitor [NEB] ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: RNA (500 ng) was reverse transcribed using a LunaScript RT SuperMix kit (New England Biolabs) according to the manufacturer’s instructions in a total 20 µL reaction mix ...
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bioRxiv - Microbiology 2022Quote: ... and 16s rRNA transcripts by the Luna Universal One-Step RT-qPCR kit (NEB, E3005), according to the manufacturer’s instructions on an Applied Biosystems 7500 Fast Real-Time PCR System ...
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bioRxiv - Immunology 2023Quote: ... Reverse transcription (RT) was performed using ProtoScript First Strand cDNA Synthesis kit (New England Biolabs) to generate cDNAs ...
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bioRxiv - Immunology 2023Quote: ... total RNA was reverse transcribed into cDNA with LunaScript RT Supermix (New England Biolabs, E3010) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... For assessment of HAC1spliced mRNA the Luna Universal Probe One-Step RT-qPCR Kit (NEB) was used with 2 µl of a 50 ng/ µl RNA sample ...
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bioRxiv - Molecular Biology 2023Quote: ... For AsiSI digestion, samples were equilibrated (2 min, RT) in 150 µL CutSmart buffer (NEB). 3µl recombinant AsiSI endonuclease (10U/µL ...
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription was performed on 0.5 µg DNase-treated total RNA using Lunascript RT (NEB) in 20µl reactions ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were run using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) on a CFX384 (Bio-Rad) ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... 1 µg RNA was reverse transcribed to cDNA (LunaScript RT SuperMix, New England BioLabs, USA).
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bioRxiv - Cell Biology 2023Quote: ... using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs, Ipswich, MA, USA) and a BIORAD CFX Opus 384 system ...
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bioRxiv - Microbiology 2023Quote: cDNA synthesis on all RNA samples was carried out using LunaScript RT SuperMix Kit (NEB) with 16 μl template RNA in 20 μl reactions according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2023Quote: ... First-strand complementary cDNA was synthesized with LunaScript™ RT SuperMix Kits (New England Biolabs). Quantitative PCR was performed with a CFX96 Touch Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Developmental Biology 2023Quote: ... and RT-qPCR was performed using the Luna Universal qPCR Master Mix (New England Biolabs). The comparative 2-ΔΔCT method was used to determine relative expression of the G9a cKO samples to compared the expression level of controls ...
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bioRxiv - Systems Biology 2020Quote: Genomic DNA libraries were prepared using the NEBnext Fast DNA Fragmentation and Library Prep Set for Ion Torrent (New England Biolabs, MA). Fragments of 450 bp size were separated and extracted with an E-Gel ...
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bioRxiv - Genetics 2021Quote: ... the sequences encoding both the PR/SET domains and full-length PRDM9 were transferred into the pMAL-c6T (New England Biolabs #N0378) vector ...
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bioRxiv - Genetics 2021Quote: ... The sRNA collected were turned into libraries using the NEBNext Multiplex Small RNA Library Prep Set (NEB #E7300S New England Biolab). The final enrichment PCR was made with 15 cycles ...
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bioRxiv - Molecular Biology 2020Quote: ... Cappable-seq and RNA-seq libraries were prepared using the NEBNext® Small RNA Library Prep Set for Illumina® (NEB), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The RNA recovered from the IP was used for cDNA library preparation using the NEB Next Small RNA Library Prep Set for Illumina (NEB, E7330S) according to the manufacturer instructions.
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bioRxiv - Developmental Biology 2019Quote: ... One replicate of each ChIP-seq library was prepared with NEBNext ChIP-seq Library Prep Master Mix Set (NEB, Cat#E6240S) using insert size selection of 250 bp ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and used this as template to construct DNA libraries using a NEBNext Fast DNA Library Prep Set for Ion Torrent kit (New England Biolabs, Canada). Each sample library contained a unique barcode adapter (NEXTflex DNA Barcodes for Ion Torrent ...
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bioRxiv - Genomics 2021Quote: This material was used for generating sequencing libraries using the Small RNA library prep Set for Illumina (Multiplex compatible) kit (NEB E7330). Typically ...
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bioRxiv - Microbiology 2020Quote: RNA recovered from the PA-ac4C-seq and PAR-CLIP procedures were used for cDNA library preparation using the NEBNext Small RNA Library Prep Set for Illumina (NEB E7330S), then sequenced using Illumina NextSeq 500 ...
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bioRxiv - Cell Biology 2021Quote: ... ChIP libraries were prepared for Illumina NextSeq 500 using NEBNext ChIP-Seq DNA Sample Prep Master Mix Set for Illumina (NEB E6240) and NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1 ...
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bioRxiv - Molecular Biology 2020Quote: ... 100ng samples were submitted for library preparation using NEBNext® Small RNA Library Prep Set for Illumina (New England Biosciences (NEB), Ipswich ...
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bioRxiv - Genomics 2019Quote: ... three sets of ligation reactions were set up by incubating 600 ng of purified digested DNA with 2 µl of high-concentration T4 DNA ligase (NEB, M0202T) overnight at 4C in a volume of 400 µl ...
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bioRxiv - Microbiology 2020Quote: ... We prepared a library with a fragment size of 300 nucleotides and used the 10 μM NEBNext Multiplex Oligos for Illumina (Set 1, NEB #E7335) lot 0091412 and the NEBNext multiplex Oligos for Illumina (Set 2 ...
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bioRxiv - Plant Biology 2021Quote: ... The sRNA libraries were constructed following manufacturer’s instruction using the NEBNext Small RNA Library Preparation set for Illumina (NEB, cat # E7300).
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bioRxiv - Evolutionary Biology 2019Quote: ... A 25 μl of reaction was set with standard 1X reaction buffer premixed with 1.5 mM MgCl2 (New England BioLabs® Inc.), 0.25mM of dNTPs (Bangalore Genei ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... purified and an indexed sequencing library was prepared using the NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina in combination with NEBNext® Multiplex Oligos for Illumina (New England Biolabs). The quality of sequencing libraries was verified on a Bioanalyzer DNA High Sensitivity chip (Agilent ...
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bioRxiv - Cancer Biology 2020Quote: ... The sequencing libraries were constructed by adding 3’ adenylation and ligating barcoded sequencing adaptors to the fragmented DNA using NEBNext DNA Library Prep Master Mix set for Illumina (NEB, E5040L). The generation of barcoded sequencing adapters can be found in 59 ...
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bioRxiv - Genomics 2021Quote: MicroRNA-seq libraries were prepared using NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs, cat. no. E7300) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: SYBR-based qPCR reactions were set up in duplicate as follows: 25 μl reactions with 20 U/ml Phusion Hot Start Flex DNA polymerase (NEB M0535L), 1X HF Buffer ...