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Citations for New England Biolabs :
1601 - 1650 of 6951 citations for rno mir 155 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... The PCR product was purified using the Monarch PCR&DNA Cleanup kit (New England BioLabs T1030L). For the last PCR ...
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bioRxiv - Molecular Biology 2022Quote: ... Post-capture PCR was performed with the NEBNext High-Fidelity 2X PCR Master Mix (NEB, M0541S) for 14-20 cycles ...
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bioRxiv - Biochemistry 2023Quote: ... PCR products were purified using the Monarch® PCR & DNA clean-up kit (New England Biolabs). In vitro expression was performed for 2 h at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... The resulting PCR products were purified using the Monarch® PCR & DNA Cleanup Kit (NEB, T1030) and used as “megaprimers” that are denatured and annealed to the original plasmid (pNG93 ...
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bioRxiv - Bioengineering 2023Quote: ... The PCR reaction was performed using NEBNext High Fidelity PCR Master Mix (NEB, catalog no. M0541L) for 20 cycles ...
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bioRxiv - Biochemistry 2023Quote: PCR mutagenesis was performed by whole plasmid PCR with Q5 high-fidelity polymerase (New England Biolabs) followed by PCR purification (Qiagen ...
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bioRxiv - Biochemistry 2023Quote: ... Each PCR consisted of: 20 μL Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB), 1 μL 10 μM forward primer ...
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bioRxiv - Synthetic Biology 2023Quote: ... General PCR for cloning was performed using the NEBNext PCR master mix (NEB cat no. M0541L), with 500 nM of forward and reverse primers ...
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bioRxiv - Evolutionary Biology 2023Quote: ... After the reaction we purified the PCR product with Monarch PCR and DNA Cleanup Kit (NEB).
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bioRxiv - Genetics 2024Quote: ... and libraries were generated by PCR with NEBNext High-Fidelity 2× PCR Master Mix (NEB, M0541). Prior to sequencing ...
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bioRxiv - Microbiology 2023Quote: ... PCR reactions were carried out using the Phusion High-Fidelity PCR Master Mix (New England Biolabs). The PCR products were selected by 2% agarose gel electrophoresis ...
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bioRxiv - Synthetic Biology 2024Quote: All PCRs were carried out using Phusion High-Fidelity PCR Master Mix with GC Buffer (NEB), gel extractions using the QIAquick Gel Extraction Kit (QIAGEN ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCRs were carried out using the Phusion High-Fidelity PCR Master Mix with GC Buffer (NEB) in a total volume of 20ul ...
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bioRxiv - Developmental Biology 2024Quote: ... DNA libraries underwent PCR amplification (NEB-Next High-Fidelity 2x PCR Master Mix, New England Biolabs), indexing using previously described primers (Buenrostro et al ...
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bioRxiv - Genetics 2021Quote: ... using Q5 polymerase PCR products (NEB). pCMV_AncBE4max (Addgene plasmid #112094 ...
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bioRxiv - Molecular Biology 2021Quote: ... USA (NEB PCR Cloning Kit E1203S) were used for the sub-cloning of DNA fragments and to create plasmid templates for run-off in vitro transcription ...
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bioRxiv - Biochemistry 2019Quote: ... The PCR amplification (Q5 polymerase (NEB)) consisted of 30 cycles (95°C ...
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bioRxiv - Genomics 2020Quote: ... 50μl PCR reactions using Q5 (NEB) were cycled 98°C 1min ...
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bioRxiv - Genetics 2021Quote: ... PCR reagents were purchased from NEB, and all primers were synthesized by Integrated DNA Technologies (IDT).
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bioRxiv - Microbiology 2021Quote: ... PCR amplification by Taq polymerase (NEB) using primers for internal regions of KL ...
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bioRxiv - Neuroscience 2021Quote: ... Luna qRT-PCR Master Mix (NEB) was used with the primers ...
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bioRxiv - Plant Biology 2021Quote: ... PCR using Standard Taq Polymerase (NEB) was performed for 30 cycles of (denaturation ...
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bioRxiv - Synthetic Biology 2021Quote: Phusion PCR reactions (New England Biolabs) were used to generate backbone fragments for library assembly ...
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bioRxiv - Synthetic Biology 2021Quote: Phusion PCR reactions (New England Biolabs) were used to generate amplicons for Illumina NGS from purified plasmid DNA ...
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bioRxiv - Molecular Biology 2021Quote: ... by PCR with Q5 polymerase (NEB) using primers petSUMO_F ...
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bioRxiv - Molecular Biology 2021Quote: ... NEB Phusion PCRs (NEB; Cat #: M0531S) were conducted from reverse transcriptase reactions generated from the SuperScript™ VILO™ cDNA Synthesis Kit (Thermofisher ...
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bioRxiv - Synthetic Biology 2020Quote: ... PCR reactions used Phusion polymerase (NEB) or PrimeStarGXL polymerase (Takara ...
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bioRxiv - Neuroscience 2020Quote: ... Luna qRT-PCR Master Mix (NEB) was used with the primers ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR was performed with OneTaq (NEB), following the manufacturer’s specifications ...
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bioRxiv - Evolutionary Biology 2021Quote: ... High-fidelity PCR Master Mix (BioLabs) and a touch-down annealing process were used for amplification ...
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bioRxiv - Microbiology 2020Quote: ... and OneTaq PCR Master Mix (NEB). Reverse primers for most sgRNAs also contained a unique KpnI or BglII site to assist clone identification by restriction fragment analysis ...
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bioRxiv - Microbiology 2021Quote: ... After PCR with Q5 polymerase (NEB), the product was treated with DpnI (NEB ...
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bioRxiv - Synthetic Biology 2021Quote: ... Phusion PCR reactions (New England Biolabs) were used to amplify these extended oligos ...
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bioRxiv - Systems Biology 2022Quote: ... and PCR master mix (NEB, M0543S). We used 12 cycles to amplify the cDNA using the following protocol ...
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bioRxiv - Genomics 2022Quote: ... DNA was extracted by NEB PCR Cleanup Kit (NEB Cat. # T1030), followed by quality control steps using Qubit fluorometer and bioanalyzer ...
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bioRxiv - Systems Biology 2022Quote: ... and PCR Master Mix (NEB, M0543S) for each reaction ...
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bioRxiv - Systems Biology 2023Quote: ... and PCR master mix (NEB, M0543S). The amplification began with a 30-second denature step at 98°C ...
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bioRxiv - Cell Biology 2023Quote: ... Following PCR using Q5 polymerase (NEB), the product is gel-purified on a 1% agarose gel ...
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bioRxiv - Cell Biology 2023Quote: All fragments were PCR purified (NEB), ligated using NEB HiFi assembly kit ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR amplification using Phusion polymerase (NEB) with selective primes (fwd ...
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bioRxiv - Developmental Biology 2024Quote: ... DNA libraries underwent PCR amplification (NEB-Next High-Fidelity 2x PCR Master Mix ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting small RNAs were used for library preparation with the NEBnext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs, Cat# E7300) following the user’s manual ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and paired-end libraries with ~300 bp inserts prepared using NEBNext DNA Library Prep Reagent Set for Illumina (New England Biolabs no. E6000L). Each library’s concentration and quality was analysed with an Agilent 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Systems Biology 2022Quote: The small RNA sequencing libraries were constructed using 3 μg total RNA per sample and NEB Next® Multiplex Small RNA Library Prep Set for Illumina® (NEB) following the manufacturer’s recommendation ...
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bioRxiv - Molecular Biology 2019Quote: ... 2x 1-5 µg of DNA were adaptor ligated and indexed using the NEBNext DNA library Prep Reagent Set (New England Biolabs: E6040S/L) and NEBNext Multiplex Oligos for Illumina Primer sets 1 (New England ...
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bioRxiv - Developmental Biology 2019Quote: ... we used index pair combinations reported for the New England Biolabs NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Pairs) set (catalog number E6440, New England Biolabs, Beverly, Massachusetts), replacing the (n)8 sequences above with the appropriate i5 and i7 barcode ...
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bioRxiv - Genomics 2019Quote: Libraries were prepared using half total volume of eluted ChIP DNA and NEBNext® DNA Library Prep Master Mix Set and Multiplex Oligos for Illumina® (New England Biolabs). Library quality was assessed using Bioanalyzer 2100 High Sensitivity DNA Gels (Agilent) ...
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bioRxiv - Immunology 2020Quote: ... libraries were prepared using NEBNext® Ultra II DNA Library Prep Master Mix Set and Multiplex Oligos for Illumina® (New England Biolabs). Library quality was assessed using Bioanalyzer 2100 High Sensitivity DNA Gels (Agilent) ...
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bioRxiv - Cancer Biology 2022Quote: The EV-RNA was compiled into cDNA libraries using a NEBNext Small RNA Library Prep Set for Illumina (Multiplex Compatible) (Cat No.: E7330, NEB, Ipswich, USA) following the manufacturer’ s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... These two combined samples were ethanol precipitated and used to generate male and female L1 small RNA libraries using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs, MA, USA). Indexed libraries were size selected for sRNAs (<150nt ...