Labshake search
Citations for New England Biolabs :
1551 - 1600 of 6951 citations for rno mir 155 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2019Quote: ... Excess bead primers without RNA molecules were removed by the treatment of Exonuclease I (NEB, NEBM0293S). Then ...
-
bioRxiv - Microbiology 2021Quote: ... the template and primers were added to a Luna Universal qPCR Master Mix (New England BioLabs) and reactions were performed on a 7500 Fast Real-Time PCR system (Life Technologies ...
-
bioRxiv - Molecular Biology 2021Quote: ... 1 μL 10uM NEBNext Index Primer (NEB E7335, NEB E7500, NEB E7710, NEB E7730, NEB E6609), 1μL 10uM NEBNext Universal PCR Primer in 20 μL total volume ...
-
bioRxiv - Molecular Biology 2021Quote: ... 1 μL 10uM NEBNext Index Primer (NEB E7335, NEB E7500, NEB E7710, NEB E7730, NEB E6609), 1μL 10uM NEBNext Universal PCR Primer in 20 μL total volume ...
-
bioRxiv - Molecular Biology 2021Quote: ... 1 μL 10uM NEBNext Index Primer (NEB E7335, NEB E7500, NEB E7710, NEB E7730, NEB E6609), 1μL 10uM NEBNext Universal PCR Primer in 20 μL total volume ...
-
bioRxiv - Molecular Biology 2021Quote: ... 1 μL 10uM NEBNext Index Primer (NEB E7335, NEB E7500, NEB E7710, NEB E7730, NEB E6609), 1μL 10uM NEBNext Universal PCR Primer in 20 μL total volume ...
-
bioRxiv - Cancer Biology 2019Quote: ... Illumina-compatible NEBNext Multiplex Dual Index Primer Pairs and adapters (New England Biolabs, Cat. No. E6440S) were ligated to the selected chimeric DNA fragments ...
-
bioRxiv - Biochemistry 2021Quote: ... primers were ordered from Integrated DNA Technologies (IDT) and phosphorylated using a New England Biosciences (NEB) phosphorylation kit (PNK enzyme ...
-
bioRxiv - Immunology 2020Quote: ... Any excess primers from the pre-amplification reaction were removed by Exonuclease-I (New England Biolabs) incubation and samples were diluted in DNA suspension buffer (Teknova ...
-
bioRxiv - Molecular Biology 2021Quote: ... Primers to amplify fragments for the assembly were designed using NEBuilder Assembly Tool (New England Biolabs). Sequences of the assembled plasmids are available upon request ...
-
bioRxiv - Molecular Biology 2021Quote: ... The purified cDNA product was then amplified with the Illumina primers by the Q5 enzyme (NEB) with the PCR program (98 °C for 30 sec ...
-
bioRxiv - Bioengineering 2022Quote: ... cDNA synthesis was performed with random primers from the Protoscript cDNA synthesis kit (NEB Cat#E6560S). Transgene expression was then quantified via qPCR using the iTaq Universal SYBR green supermix and primers binding to the mCherry transcript ...
-
bioRxiv - Immunology 2022Quote: ... The mutagenesis was performed according to the substitution protocol using primers designed with the NEBaseChanger (NEB). Codon-swapped EGFP expression vectors were produced previously59 ...
-
bioRxiv - Genomics 2022Quote: ... 2 μL of 10 mM i5 primer and 25 μL of 2x NEBNext Master Mix (NEB). The following PCR conditions were used ...
-
bioRxiv - Molecular Biology 2022Quote: ... complementary DNA (cDNA) was synthesized using ProtoScript® II Reverse Transcriptase with random primer mix (NEB). This cDNA was also used for a conventional PCR using Go-Taq® Master Mix (Promega ...
-
bioRxiv - Synthetic Biology 2022Quote: ... second-strand synthesis reactions were conducted using the primer sgRNA-Rev2 and T4 DNA polymerase (NEB), gel extracted ...
-
bioRxiv - Developmental Biology 2022Quote: ... Genotyping for maintenance of lines was performed with primers JS137/JS138 and enzyme BslI (NEB R0555S) for dmrt2aumb13 ...
-
Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capturebioRxiv - Synthetic Biology 2023Quote: ... Each variant was amplified with primers PK421&422 using Q5 High-Fidelity 2X Master Mix (NEB) in a 200 µl reaction followed by DpnI digestion for 2 h ...
-
bioRxiv - Microbiology 2023Quote: ... psfx94-3xFlag was constructed from psfx94 using primer pair pAH56_3xFlag.F/94sfx_3xFlag.R and the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2022Quote: ... we extended the primer to generate double-strand DNA with Klenow Large fragment mix (1X NEB Buffer 2 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Illumina-compatible NEBNext Multiplex Dual Index primer pairs and adapters (New England Biolabs, cat. no. E6440S) were added to each sample ...
-
bioRxiv - Microbiology 2023Quote: ... 2 µM each of gene- specific forward and reverse primers and 1 unit Phusion polymerase (NEB) were mixed in 1X HF Phusion buffer and 3% (v/v ...
-
bioRxiv - Physiology 2023Quote: ... using Luna Universal qPCR master mix for Sybr Green primers (New England Biolabs, Cat. No. M3003X). All primer sequences are listed in Supplementary Table 1 ...
-
bioRxiv - Microbiology 2023Quote: ... aeruginosa strains using the primers indicated in Table S7 with Q5 DNA Polymerase (New England Biolabs), in reactions that added regions of homology to plasmid pUCP20 ...
-
bioRxiv - Immunology 2022Quote: ... and 0.33 μM nested reverse primers (IgG:NNNNNSGATGGGCCCTTGGTGGARGC; IgM:NNNNGGTTGGGGCGGATGCACTCC;IgK:NNNNNNGGGAAGATGAAGACAGATGGT, IgL:NNNNNNGGGYGGGAACAGAGTGACC) with Q5 DNA Polymerase (New England Biolabs) in a 100 μl reactions ...
-
bioRxiv - Cancer Biology 2023Quote: ... and NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs, NEB, E6440) as per manufacturer’s recommendation ...
-
bioRxiv - Neuroscience 2023Quote: ... the tagmented DNA was amplified using Illumina primers (NEBNext® Multiplex Oligos for Illumina, NEB #7335S) with the following cycling conditions ...
-
bioRxiv - Molecular Biology 2023Quote: ... and the NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs; New England BioLabs). Libraries were checked for quality using the Qubit dsDNA HS Assay kit and TapeStation High Sensitivity DNA assay kit ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... with NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) (New England Biolabs E6440S) and polyA enrichment module (NEB E7490S) ...
-
bioRxiv - Cancer Biology 2023Quote: ... NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs, New England Biolabs) were used ...
-
bioRxiv - Cell Biology 2023Quote: ... 45-72°C for 30 seconds (depending on melting temperature of primer pairs determined by NEB Tm calculator https://tmcalculator.neb.com) ...
-
bioRxiv - Developmental Biology 2023Quote: ... in conjunction with NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) (NEB #E6440S), according to the manufacturer’s instructions ...
-
bioRxiv - Genetics 2023Quote: ... All genotyping was done with 10 μM primers and using OneTaq 2X master mix (NEB M0482) according to the manufacturer’s recommendations ...
-
bioRxiv - Microbiology 2023Quote: ... cDNA was synthesized using random primers with 5x Protoscript II Reverse Transcriptase (New England BioLabs Ltd). cDNA was amplified using the VSG Splice Leader and SP6-VSG14mer primers (41 ...
-
bioRxiv - Microbiology 2023Quote: ... primers for lacZ-LAupp: lacZupp_intF2 and lacZupp_intR2) and assembled using NEB Gibson Master Mix (NEB #E2611). 10 µL of the Gibson reaction was used to transform competent EC1000 cells ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... The PCR products from all reactions were purified with the Monarch PCR & DNA Cleanup Kit (NEB) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... Desired PCR cycle numbers were determined in test endpoint PCRs using Q5 DNA polymerase (NEB M0491L). Final HiC libraries were generated from 4-6 individual PCR reactions ...
-
bioRxiv - Genetics 2021Quote: ... and libraries were generated by PCR with NEBNext High Fidelity 2X PCR Master Mix (NEB M0541). Library size was confirmed on a BioAnalyzer prior to sequencing on the NextSeq 550 (40bp read length ...
-
bioRxiv - Cell Biology 2020Quote: ... The PCR reaction was column-purified using the Monarch PCR & DNA Cleanup Kit (New England BioLabs). The IVT reaction including DNAseI digest was performed with the mMESSAGE mMACHINE T7 Transcription Kit (Invitrogen ...
-
bioRxiv - Microbiology 2019Quote: ... All PCR reactions were carried out with Phusion® High-Fidelity PCR MasterMix (New England Biolabs). The PCR products examined with 400-450bp were chosen and mixed in equal density ratios ...
-
bioRxiv - Neuroscience 2022Quote: PCR products were extracted with Monarch PCR & DNA Gel Cleanup Kit (New England Biolabs, Ipswich, MA) and sequenced by ABI 3730 automated DNA sequencer.
-
bioRxiv - Molecular Biology 2022Quote: ... Site-directed mutagenesis was performed by PCR with NEBNext High-Fidelity 2x PCR master mix (NEB) using overlapping primers with 15 nt overhangs carrying the desired mutation (IDT).
-
bioRxiv - Neuroscience 2021Quote: ... Kv1.2wt-T2A and Kir2.1wt-T2A fragments were magnified by PCR (Phusion High-Fidelity PCR Kit, NEB) from pcDNA3-Kv1.2wt (human cDNA template ...
-
bioRxiv - Genetics 2022Quote: ... Each PCR consisted of: 20 µL Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB), 2 µL 10 µM i5 indexing adapter ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... PCR products were generated from genomic DNA with a Phusion® High-Fidelity PCR kit (NEB) and primers that introduced KpnI and XhoI restriction sites ...
-
bioRxiv - Immunology 2020Quote: ... PCR was performed with a Phusion High-Fidelity PCR Kit (New England Biolabs, Ipswich, MA, USA) in 20µL reactions mixed with 2µL cDNA and 10µM primers ...
-
bioRxiv - Synthetic Biology 2019Quote: ... transcription templates were generated by PCR amplification (Phusion High-Fidelity PCR Kit, New England Biolabs #E0553) of the plasmids to include a 5’ region upstream of the T7 promoter and a 3’ region ending with either 3WJdB or the T7 terminator with the primers listed in Supporting Data File 1 ...
-
bioRxiv - Microbiology 2020Quote: ... and PCR mixes using the Q5® High-Fidelity PCR Kit (New England Biolabs, MA, USA) following the manufacturer’s instructions ...
-
bioRxiv - Animal Behavior and Cognition 2021Quote: ... All PCR reactions were carried out with Phusion High-Fidelity PCR Master Mix (New England Biolabs). The PCR products were mixed with an equal volume of 1× loading buffer (containing SYB green ...
-
bioRxiv - Genetics 2021Quote: ... PCR products were purified using NEBMonarch PCR and DNA Clean-up Kit (New England Biolabs, USA), following the manufacturer’s instructions ...