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Citations for New England Biolabs :
1251 - 1300 of 7437 citations for rno mir 155 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... the NEB Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) was used and the qPCR was conducted on the StepOnePlus Real-Time PCR System (Thermo Fisher Scientific)44 ...
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bioRxiv - Microbiology 2023Quote: ... The Luna Universal One-Step RT-qPCR Kit (New England Biolabs, Ipswich, MA) was used ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was synthsised using using LunaScript™ RT SuperMix Kit (New England Biolabs) before analysis via qRT-PCR ...
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bioRxiv - Neuroscience 2023Quote: ... This mix includes 2.5 µL of Template Switching RT Buffer (NEB Catalog# B0466S), 0.5 µL of 25 µM Template Switching Oligo (5 µM final concentration in RT mix ...
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bioRxiv - Molecular Biology 2023Quote: ... For these analyses Luna Universal One-Step RT-qPCR Kit (New England Biolabs) on a CFX opus real-time 384 system (BioRad ...
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bioRxiv - Bioengineering 2023Quote: The Luna Universal Probe One-Step RT-qPCR kit (New England Biolabs, USA) was used ...
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bioRxiv - Genomics 2023Quote: ... RNA was converted to cDNA using LunaScript RT SuperMix (New England Biolabs, 3010) in a final 10 µL reaction ...
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bioRxiv - Biochemistry 2024Quote: ... The total RNA was reverse transcribed using the LunaScript RT SuperMix Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Thawed RNA specimens were converted to cDNA using the Luna RT mix (NEB). Arctic multiplex PCR primer pools (IDT ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA was synthesised using the LunaScript RT SuperMix Kit (New England Biolabs, E3010L) as per manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: ... Two independent lines were successfully established and PCR validated through single fly PCR (Gloor et al., 1993) using OneTaq PCR master mix (NEB #M0271L). Primers for PCR were designed to flank the integration site (primer sequences ...
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bioRxiv - Genomics 2024Quote: ... A synthesized TWIST oligo pool with 300 bp long elements (270 bp insert + 2*15 bp PCR handles) was amplified by 12-cycle PCR using NEBNext High-Fidelity 2x PCR Master Mix (New England BioLabs, M0541L), the forward primer 5BC-AG-f01 and reverse primer 5BC-AG-r01 were used to add the minimal promoter ...
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bioRxiv - Microbiology 2020Quote: ... we designed primers for cloning into the pLEX-MCS vector using Gibson Assembly (NEB). To amplify ETV7 ...
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bioRxiv - Molecular Biology 2021Quote: ... indexed using NEBnext Multiplex Oligos for Illumina Dual Index Primers (New England Biolabs E7600S) and sequenced simultaneously using a Illumina HiSeq4000 System.
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bioRxiv - Biophysics 2021Quote: ... Primer extension was initiated by the addition of AMV reverse transcriptase (New England Biolabs) and allowed to proceed for 10 min ...
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bioRxiv - Biochemistry 2021Quote: ... the free primer was removed by Exonuclease I (80 U; M0293S, NEB, Ipswich, MA), and a 3’ DNA adaptor was ligated onto the cDNA product with T4 RNA Ligase 1 ...
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bioRxiv - Microbiology 2020Quote: ... Annealed primers were then diluted to 1 µl in 200 µl and ligated (NEB) to the digestedand dephosphorylated Cas9 vector.
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bioRxiv - Neuroscience 2020Quote: ... generated template DNA by primer annealing with the NEBNext High-Fidelity polymerase (NEB, M0541S), and carried out in vitro transcription using the HiScribe T7 Kit (NEB ...
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Micro RNAs are minor constituents of extracellular vesicles and are hardly delivered to target cellsbioRxiv - Cell Biology 2020Quote: The primers were annealed and ligated with psiCHECK2 digested with XhoI and NotI (NEB). The pCDH vectors harboring single EBV miRNAs were used as described (Tagawa et al. ...
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bioRxiv - Genomics 2022Quote: ... NEBNext Multiplex Oligos for Illumina® Dual Index Primers (cat. #E7600, New England Biolabs), and AMPure XP Beads (cat ...
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bioRxiv - Plant Biology 2022Quote: ... cDNA was generated with oligo dT18 primer using the Protoscript II reverse transcriptase (NEB) in the presence of Murine RNase inhibitor (NEB) ...
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bioRxiv - Microbiology 2021Quote: ... was used to generate single-strand cDNA using random primer mix (NEB, Hitchin, UK). ARTIC V3 PCR amplicons from the single-strand cDNA were generated following the Nanopore Protocol of PCR tiling of SARS-CoV-2 virus (Version ...
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bioRxiv - Neuroscience 2021Quote: ... Mutagenesis primers were designed using the “substitution” feature in NEBaseChanger v1.2.9 (New England Biolabs). Mutagenesis occurs at nucleotide 805-807 (GGA→ GAA ...
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bioRxiv - Immunology 2022Quote: ... The oligonucleotide was used with primer R1(RB202)* in a NEBuilder assembly reaction (NEB). The resulting plasmid was transformed in electro-competent E ...
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bioRxiv - Plant Biology 2020Quote: ... 1μL of each primer and 2U Phusion® High-Fidelity DNA Polymerase (NEB, USA) for 35 cycles (98 °C for 30s ...
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bioRxiv - Systems Biology 2019Quote: ... The RNA and free primers were degraded using 20U of Exonuclease I (NEB, M0293L) and 50U RNaseIf (NEB ...
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bioRxiv - Synthetic Biology 2020Quote: ... 6 μl primers (10 μM) and 50 μl Q5 high fidelity master mix (NEB). The thermocycling parameters were ...
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bioRxiv - Synthetic Biology 2021Quote: ... Primers were ordered from IDT with Q5 Tms (calculated via the NEB Tm calculator) of 70-72 degrees ...
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bioRxiv - Synthetic Biology 2020Quote: ... This was done in 50 μl reactions using 3 μl dual index primers (NEB), 135 ng purified PCR product from the previous reaction and 25 μl Q5 high fidelity master mix (NEB) ...
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bioRxiv - Microbiology 2021Quote: ... was used to generate single-strand cDNA using random primer mix (NEB, Hitchin, UK). ARTIC V3 PCR amplicons from the single-strand cDNA were generated following the Nanopore Protocol of PCR tiling of SARS-CoV-2 virus (Version ...
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bioRxiv - Genetics 2020Quote: ... amplified using primers with KpnI and BamHI RE sites with Phusion Master Mix (NEB), digested ...
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bioRxiv - Genomics 2021Quote: ... cDNA was prepared from viral RNA using random primer mix (NEB, Massachusetts, United States), and LunaScript® RT SuperMix Kit (Cat# E3010G) ...
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bioRxiv - Cell Biology 2022Quote: ... and Calnexin (membrane) using qPCR primers (IDT) with Q5 polymerase (New England Biolabs, M0492) was measured with a highly sensitive dsDNA detection dye (Lumiprobe ...
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bioRxiv - Developmental Biology 2022Quote: ... with SP6 and T3 primers and Phusion High Fidelity DNA polymerase (New England, BioLabs). The purified product was used as a template for the transcription reaction using the mMessage mMachine SP6 RNA transcription kit (AM1340 ...
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bioRxiv - Microbiology 2022Quote: ... These primer pairs were assembled by heat-denaturation and annealing in Buffer 3.1 (NEB), then cloned into BbsI-linearized pX458 (62 ...
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bioRxiv - Biochemistry 2023Quote: ... Primers for generating mutations for MsbA were designed using the online tool NEBaseChanger (NEB) and carried out using the KLD enzyme mix (NEB ...
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bioRxiv - Neuroscience 2024Quote: ... and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) (NEB E6440S). Library cleanup was performed prior to and after PCR amplification using 0.8X Kapa Hyperpure beads (Roche 08963851001) ...
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bioRxiv - Genomics 2023Quote: ... Excessive primers were digested with the addition of 0.5 μL Exonuclease I (NEB M0293S), and incubation at 37°C for 30 min and 72°C for 20 min ...
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bioRxiv - Synthetic Biology 2022Quote: ... The DNA template for mega primer generation was amplified using Q5 DNA polymerase (NEB) and the T7 and T7term primer from EOY4D2.1@pet21(+) ...
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bioRxiv - Biochemistry 2022Quote: ... using the Q5 site-directed mutagenesis kit and primer design tools (New England BioLabs) with the following primer pair:
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bioRxiv - Genetics 2023Quote: ... 200 nM target primers and 10 U of Hind III–HF restriction enzyme (NEB) in a final volume of 20 μL ...
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bioRxiv - Microbiology 2023Quote: ... 0.5 µM of each primer and 1.25 U Taq DNA polymerase (New England Biolabs). DNA amplification was performed in a MyCycler thermal cycler (BioRad ...
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bioRxiv - Cancer Biology 2023Quote: ... amplification of extracted gDNA by PCR using a barcoded forward primer (CC_fwd1 to CC_fwd20) and a barcoded reverse primer (CC_Cri_a_rev1 to CC_Cri_a_rev20) using Q5 polymerase (NEB M0491L). Pooled libraries were sequenced on an Illumina NextSeq 500 platform using a 75 bp single read on a high output flow cell with a 2–5% PhiX spike-in ...
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bioRxiv - Molecular Biology 2023Quote: ... and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) (NEB #E6440S). Library cleanup was performed prior to and after PCR amplification using 0.8X Kapa Hyperpure beads (Roche #08963851001) ...
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bioRxiv - Cell Biology 2023Quote: ... using primers synthesized by IDT and a Q5® Site-Directed Mutagenesis Kit (NEB).
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bioRxiv - Neuroscience 2023Quote: ... 10 µM of each primer was combined with OneTaq QuickLoad Master Mix (NEB #M0486) and run in a thermocycler as follows ...
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bioRxiv - Molecular Biology 2023Quote: ... the adaptor complementary primer was labeled with [γ-32P]-ATP and T4 PNK (NEB) using the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... The annealed primers were extended to fully double stranded DNA using Klenow polymerase (NEB). The barcode was purified by phenol-chloroform extraction ...
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bioRxiv - Molecular Biology 2023Quote: ... Excess primers were degraded by adding 0.5 μL of thermolabile exonuclease I (NEB m0568) at 37 °C for 15 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... reactions were performed with mutagenesis primers and the Q5 DNA polymerase (New England Biolabs). Afterward ...