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Citations for New England Biolabs :
101 - 150 of 8872 citations for RPA kit reagent since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... and 3 μL of Uracil-Specific Excision Reagent (USER) Enzyme (NEB, Beijing, China) was then used with the size-selected and adaptor-ligated cDNA at 37°C for 15 min ...
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bioRxiv - Synthetic Biology 2020Quote: ... SNAP reagents (BG-649, BG-fluorescein and BG-biotin) were obtained from NEB. Electrocompetent cells were used in all transformations.
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bioRxiv - Molecular Biology 2020Quote: ... mRuby2 was inserted using the HiFi DNA Assembly reagent (New England Biolabs Inc).
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bioRxiv - Microbiology 2021Quote: ... The product was digested using the KLD Enzyme Mix reagents (New England Biolabs) and transformed into DH5-alpha competent cells ...
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bioRxiv - Genetics 2021Quote: ... and all molecular cloning reagents were purchased from New England Biolabs (NEB, USA). Afatinib (S1011) ...
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bioRxiv - Synthetic Biology 2022Quote: All restriction enzymes and cloning reagents were purchased from New England Biolabs (NEB) unless specified otherwise ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was prepared for sequencing on the Illumina platform using Next Reagents (NEB) and Agencourt AMPure XP beads (Beckman Coulter).
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bioRxiv - Molecular Biology 2023Quote: ... DNA was prepared for sequencing on the Illumina platform using Next Reagents (NEB) and Agencourt AMPure XP beads (Beckman Coulter).
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bioRxiv - Genomics 2023Quote: ... and Uracil-Specific Excision Reagent (1 U/μl USER, catalog num. M5505L, NEB) treatment ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We repaired the DNA with USER (Uracil-Specific Excision Reagent) Enzyme (NEB, USA) for deanimation ...
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bioRxiv - Developmental Biology 2023Quote: ... were ligated using NEBNext DNA Sample Prep Reagent Set 1 (New England Biolabs). Immunoprecipitations were carried out using 500ng DNA per sample ...
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bioRxiv - Developmental Biology 2023Quote: ... the One Taq HS QuickLoad PCR reagent was used (Catalog no. M0488L, NEB). The genotyping process utilized the primer sets and cycling conditions listed in Supplementary Table 1.
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bioRxiv - Cell Biology 2023Quote: ... the products were treated with uracil-specific excision reagent (USER) enzyme (NEB, N5505S) to remove the forward primer ...
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bioRxiv - Developmental Biology 2024Quote: ... The sorted spermatid fractions were collected in Monarch DNA/RNA protection reagent (NEB) and flash frozen in liquid nitrogen before the RNA extraction.
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bioRxiv - Synthetic Biology 2021Quote: ... These reaction solutions included the same reagents as the commercial reaction buffer from NEB but without Tris-HCl ...
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bioRxiv - Immunology 2022Quote: We used NEBNext Ultra II RNA Prep reagents and multiplex oligos (New England Biolabs) to prepare Illumina-ready libraries ...
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bioRxiv - Molecular Biology 2020Quote: ... and inserted into the opened plasmid with NEBuilder reagents (New England Biolabs; Berverley, MA) to create plasmid pBbRad51NLHAglmS (pBRNLHg ...
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bioRxiv - Neuroscience 2022Quote: ... and subjected to Illumina library preparation using NEBNext reagents (Cat#: E6040, New England Biolabs). The quality and size distribution of the Illumina libraries were determined using an Agilent Bioanalyzer2100 ...
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bioRxiv - Genomics 2022Quote: ... the probes were subjected to uracil-specific excision reagent (USER) enzyme (NEB, Cat. N5505S) treatment for ∼24 h at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-qPCR was performed using Luna Universal One-Step RT-qPCR reagents (NEB, E3005) and the primers listed in Table S3.
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bioRxiv - Microbiology 2023Quote: Major reagents involved in this study included EnGen LbCas12a (Cpf1) (New England BioLabs, M0653T), plasmid mini kit I (OMEGA ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.66 µl Luna Cell Ready RNA Protection Reagent 25X (New England Biolabs, Ipswich, MA), 0.66 µl Luna Cell Ready Protease 25X ...
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bioRxiv - Bioengineering 2023Quote: ... and cloned into a pHen6c vector by Gibson assembly using commercially-available reagents (NEB) [46] ...
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bioRxiv - Biochemistry 2023Quote: ... Samples of E.coli cultures were harvested and resuspended in E.coli lysis reagent (NEB, US). Lysates and purified proteins were separated by 4-12% Bis-Tris SDS-PAGE (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... 500 µL PCR reagents containing 1.67X Q5® High-Fidelity Master Mix (NEB, M0515), 0.625 mg/mL BSA ...
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bioRxiv - Genomics 2022Quote: Ribosomal RNA depletion kit (rRNA Depletion Kit, E6310L, NEB)
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bioRxiv - Immunology 2021Quote: ... Illumina-ready libraries were generated using NEBNext Ultra II RNA Prep reagents (New England BioLabs) as previously described18 ...
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bioRxiv - Microbiology 2020Quote: ... qPCR was performed using LUNA SYBR green qPCR reagents (New England Biolabs, Ipswich, MA, USA) using primers in the table ...
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bioRxiv - Genomics 2021Quote: ... the NEBNext Ultra II Non-directional RNA Second Strand Synthesis Buffer and Reagents (NEB Biolabs) was used for the second strand synthesis.
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bioRxiv - Genomics 2021Quote: ... the NEBNext Ultra II Non-directional RNA Second Strand Synthesis Buffer and Reagents (NEB Biolabs) was used for the second strand synthesis.
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bioRxiv - Microbiology 2022Quote: ... PCR master mix reagents (see Figure 4): Taq polymerase and 10X Reaction Buffer (NEB M0273S), nucleotide mix containing 10 mM dTTP ...
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bioRxiv - Immunology 2022Quote: ... Illumina-ready libraries were generated using NEBNext Ultra II RNA Preparation reagents (New England BioLabs). The barcoded Illumina-ready libraries were sequenced using paired-end 151-base protocol on either a NovaSeq 6000 (Illumina ...
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bioRxiv - Bioengineering 2023Quote: ... and subcloned into a pY71A(lc) vector by Gibson assembly using commercially-available reagents (NEB) [46].
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bioRxiv - Genomics 2024Quote: ... were then added using the NEBNext Sample Reagent Set (New England Biolabs, Ipswich, MA, USA) and purified using Ampure XP (Beckmann Coulter Genomics ...
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bioRxiv - Microbiology 2023Quote: ... Monarch kit (NEB) extracted and 0.1 pmol of each DNA fragment was CPER amplified in a 50 µL reaction containing 200 µM of dNTPs ...
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bioRxiv - Neuroscience 2019Quote: ... and then mounted using 10 μl ProLong Gold Antifade Reagent containing DAPI (9071S, New England Biolabs). The slides were imaged when the mounting medium was fully cured >12 h.
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bioRxiv - Genomics 2020Quote: ... gDNA is “repaired” using the NEBNext FFPE DNA Repair reagents (M6630, New England Biolabs Ipswich Ma) followed by dA-tailed using the NEBNext End Repair/dA-tailing module ...
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bioRxiv - Molecular Biology 2022Quote: ... RT-qPCR was performed by using Luna Universal One-Step RT-qPCR reagents (New England Biolabs). Primers targeting the MS2 stem-loop regions were used for quantifying repeat RNA expression levels ...
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bioRxiv - Synthetic Biology 2021Quote: ... Reagents added were 1 μl of 1 mg/ml BSA (diluted from 20 mg/ml - NEB), 1 μl T4 DNA ligase buffer (NEB or Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... Cloning reagents were obtained as follows: Restriction enzymes and DNA ligase (New England Biolabs; Ipswich, MA); Primestar HS DNA polymerase (Takara Bio USA ...
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bioRxiv - Neuroscience 2023Quote: ... RT-qPCR was performed by using Luna Universal One-Step RT-qPCR reagents (New England Biolabs), following the manufacturer’s instructions.
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bioRxiv - Plant Biology 2024Quote: ... Mixed tissue sections and reaction reagents: 2 μl 10× Tag DNA polymerase (NEB, Cat. No. M0267V), 0.4 μL Buffer Tag DNA polymerase (5 U/μL ...
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bioRxiv - Microbiology 2020Quote: ... polymerases and Gibson mix reagents were used according to the manufacturer’s instructions (Promega and New England Biolabs). Plasmids were verified by PCR and Sanger sequencing (Eurofins).
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bioRxiv - Cell Biology 2020Quote: ... Libraries were prepared with NebNext® ChIP seq reagents for Illumina (E6240 and E7335, New England Biolabs), following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... Total mRNA was prepared from 1 μg total RNA using poly(A) mRNA magnetic isolation reagents (NEB) and fragmented at 94 °C for 15 min ...
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bioRxiv - Bioengineering 2022Quote: ... Sub-cloning and other modifications to plasmids were also performed with Gibson Assembly using reagents from NEB. Assemblies were transformed into NEB Stable E ...
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bioRxiv - Plant Biology 2022Quote: ... RNA was extracted using TRIzol reagent (MRC, USA) and ligated with T4 RNA ligase 1 (NEB, USA) at 37 °C for 1 h ...
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bioRxiv - Immunology 2024Quote: ... Transcript expression was analyzed via reverse transcriptase quantitative real-time PCR using Lunaprobe reagent (New England Biolabs) on a Quantstudio3 thermocycler (ABI) ...
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bioRxiv - Neuroscience 2021Quote: ... T7 HiScribe kit (NEB) was used to transcribe the sgRNA DNA template with the appended T7 promoter ...
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bioRxiv - Biophysics 2020Quote: The transient transfection of HEK293 cells was performed67 using the TransFectin Lipid Reagent (Bio-Rad) (New England Biolabs). Regularly ...