Labshake search
Citations for New England Biolabs :
401 - 450 of 8872 citations for RPA kit reagent since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Samples with RIN value higher than 8 were processed using the NEBNext poly(A) mRNA Magnetic Isolation kit and the NEBNext Ultra II RNA Library Prep kit for Illumina (New England Biolabs), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... PCR/reaction cleanup and gel extraction were conducted with the Monarch PCR and DNA cleanup kit and Monarch DNA gel extraction kits (NEB). E ...
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bioRxiv - Microbiology 2021Quote: ... cholerae and phages was extracted using commercially available kits (Qiagen DNeasy Blood and Tissue or Monarch genomic DNA purification kit (New England BioLabs). For preparation of phage DNA ...
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bioRxiv - Microbiology 2022Quote: ... After enrichment of mRNA using the Universal Ribodepletion Kit cDNA preparation was performed with the Next Ultra Directional Library Preparation Kit for Illumina (NEB). The cDNA was sequenced on HiSeq 3000 (Illumina ...
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bioRxiv - Cell Biology 2022Quote: ... 60 ng of total RNA was used for library preparation using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina with NEBNext rRNA Depletion Kit (New England Biolabs). The libraries were paired-end sequenced (2 × 75 bp ...
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All-trans retinoic acid induces synaptopodin-dependent metaplasticity in mouse dentate granule cellsbioRxiv - Neuroscience 2021Quote: ... and RNA was consecutively isolated using a column based RNA isolation kit according to the manufacturer’s instructions (Monarch® Total RNA Miniprep Kit; #T2010S New England Biolabs). Strand specific cDNA library preparation from polyA enriched RNA (150 bp mean read length ...
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bioRxiv - Immunology 2019Quote: ... The culture supernatant was projected to perform luciferase assay with Gaussia Luciferase assay kit and Cypridina Luciferase assay kit (NEB) following their description.
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bioRxiv - Systems Biology 2021Quote: Bulk RNA-seq libraries were prepared from 1000 ng of RNA using the NEBNext Ultra RNA Library Prep kit for Illumina or the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Enrichment of mRNA was done using the Universal Ribodepletion Kit followed by Next Ultra Directional Library Preparation Kit for Illumina (NEB). The cDNA was sequenced on HiSeq™ 2500 (Illumina ...
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bioRxiv - Genomics 2021Quote: ... Libraries were constructed from 1 µg of total RNA using Illumina’s TruSeq stranded mRNA Library Preparation Kit or from 500 ng total RNA using NEBNext Ultra II RNA -polyA+ (mRNA) Library Preparation Kit (New England Biolabs) following the manufacturer’s description.
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bioRxiv - Genetics 2021Quote: ... RNA samples were depleted of rRNA using the QIAseq FastSelect RNA Removal Kit then processed into libraries using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Samples were recovered by phenol/CHCl3/isoamyl alcohol extraction twice and purified as per kit instruction using Monarch PCR and DNA cleanup Kit (New England BioLabs). DNA was suspended in 200 ul pre-warm (55°C ...
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bioRxiv - Developmental Biology 2021Quote: ... or deletions in full-length Gpr161 were generated using Quikchange site-directed mutagenesis kit (Stratagene or Q5 Mutagenesis Kit (NEB).
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bioRxiv - Molecular Biology 2021Quote: ... RNA-sequencing libraries were prepared with a NEBNext rRNA Depletion Kit and an ENBNext Ultra Directional RNA Library Prep Kit (New England Biolabs). Then ...
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bioRxiv - Genomics 2022Quote: MNase-seq libraries were prepared from purified nucleosomal DNA using the kit NEBNext Ultra DNA Library Prep Kit for Illumina (cat. # E7370L New England BioLabs) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: RNA was purified by silica-based SPE using Monarch RNA Cleanup Kits (for in vitro assays) or Total RNA Miniprep Kit for intracellular samples (NEB).
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bioRxiv - Plant Biology 2022Quote: ... Total RNA was isolated using Qiagen RNeasy Plant Mini Kit and cDNA was synthesised using ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs). SYBR Green Power Up (Thermofisher ...
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bioRxiv - Plant Biology 2023Quote: ... from 100 to 1000 ng of total RNA using a stranded kit (Illumina TruSeq Stranded Total RNA Kit or NEB Next Ultra™ II Directional RNA Library Prep Kit for Illumina) ...
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bioRxiv - Cell Biology 2023Quote: ... In vitro transcription was performed with either HiScribe T7 ARCA mRNA kit or the HiScribe In Vitro Transcription Kit (NEB) according to the manufacturer’s recommendation ...
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bioRxiv - Genomics 2023Quote: ... libraries were prepared either with Nugen Ovation Ultralow V2 DNA-Seq library prep kit (NuGen) or NEBNext Ultra II DNA library prep kit for Illumina (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Capped RNA was synthesized from the pCS2+hSpCas9 vector using an AmpliCap SP6 High Yield Message Maker Kit (Cell Script) and then purified with a Monarch RNA Cleanup Kit (50 µg) (New England BioLabs).
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bioRxiv - Neuroscience 2023Quote: RNA was extracted using the QIAzol and the NEB Monarch RNA extraction kit and ribosomal RNA was depleted using the NEBNext rRNA depletion kit (NEB) ...
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bioRxiv - Microbiology 2023Quote: ... Ribosomal RNA was depleted using the Illumina Ribo-Zero Plus rRNA Depletion kit followed by library preparation with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) as per manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... was extracted via the UHMW DNA extraction protocol of the Nanobind CBB Big DNA kit (Circulomics) or via the Monarch HMW DNA Extraction Kit for Cells & Blood (New England Biolabs) and quantified using Qubit dsDNA Broad Range kit (ThermoFisher) ...
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bioRxiv - Cell Biology 2024Quote: ... and areas corresponding to the expected size were excised and purified from the gel using a gel extraction kit (Monarch DNA Gel Extraction Kit, NEB). Following purification ...
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bioRxiv - Microbiology 2024Quote: ... The extracted nucleic acid was then subject to library construction using NEBNext Ultra RNA Library Prep Kit and NEB Next Ultra DNA Library Prep Kit (LTD.NEB, China) for RNA and DNA samples ...
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bioRxiv - Cancer Biology 2021Quote: ... Ligations were performed using Quick Ligation Kit (NEB). Reaction mixes were incubated for 5 min at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... using Quick Ligation Kit (NEB; Cat. No. M2200L). The ligated DNA was PCR amplified with primers containing Illumina paired-end sequencing adaptors ...
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bioRxiv - Developmental Biology 2021Quote: ... we used the EnGen sgRNA synthesis kit (NEB) following the manufacturer’s instructions with the following targeting sequences tnpo1 sgRNA#1 (5’-GGCATGGGGGCCACCTCTTG-3’) ...
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bioRxiv - Developmental Biology 2021Quote: ... NEBNext Ultra II DNA Library Prep Kit (NEB) with 11 cycles of PCR was used to prepare sequencing libraries ...
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bioRxiv - Microbiology 2022Quote: ... treatment and purification using Monarch Cleanup Kit (NEB). RNA copy number was calculated from its molecular weight and absorbance measured by a NanoDrop 1000 (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... PolyA selection/enrichment with Ultra II Kit (NEB) was the method adopted for library preparation.
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bioRxiv - Neuroscience 2021Quote: ... Q5® Site-Directed Mutagenesis Kit (NEB, Inc.) was used to make point mutations to produce five individual EA6-related amino acid substitutions (M128R ...
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bioRxiv - Genomics 2020Quote: ... prepared using the NEBNext kit (New England Biolabs) following the manufacturer’s directions ...
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bioRxiv - Genomics 2020Quote: ... and ribodepleted using the rRNA depletion kit (NEB). The library was then prepared using the NEBNext Ultra II direction Library preparation kit (NEB ...
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bioRxiv - Genomics 2020Quote: ... and the Q5 site-directed mutagenesis kit (NEB) to recreate 4 amino acid changes that were previously found to improve the robustness of tagmentation to lower DNA concentrations (41) ...
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bioRxiv - Neuroscience 2019Quote: ... The NEB Next rRNA Depletion Kit (NEB #E6310X) was used to process 250 ng of total RNA ...
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bioRxiv - Biophysics 2019Quote: ... or pMiniT (NEB PCR cloning kit, Cat. # E1202S) plasmids and sequenced ...
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bioRxiv - Molecular Biology 2020Quote: ... blunting with the Quick Blunting™ Kit (NEB), and circularization with T4 Ligase (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... PCRs were performed using Q5 polymerase kit (NEB) in a Perkin- MJ Research PTC-200 Peltier Thermal Cycler or C1000 Touch™ Thermal Cycler (BioRad) ...
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bioRxiv - Molecular Biology 2019Quote: ... the NEB Q5 Site-Directed Mutagenesis Kit (NEB) was used according to manufacturers protocol to introduce silent mutations in the N-cad siRNA1 targeting sequence ...
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bioRxiv - Genetics 2020Quote: ... and purified using a PCR purification kit (NEB).
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bioRxiv - Developmental Biology 2020Quote: ... were ligated with Quick Ligation kit (NEB, M2200S). The libraries then were amplified using PCR Enhancer mix and primer cocktail (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... purified using Monarch Gel Extraction Kit (NEB, USA) and ligated with SmaI-digested and dephosphorylated pUC19 vector using Blunt/TA Ligase Mastermix (NEB ...
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bioRxiv - Synthetic Biology 2021Quote: ... isolated using the Monarch Plasmid preparation kit (NEB) and verified by sequencing (Source Bioscience ...
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bioRxiv - Bioengineering 2021Quote: ... using an NEBuilder HiFi DNA assembly kit (NEB). These manipulations generated px552-pegRNA-CAG-mCherry.
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bioRxiv - Bioengineering 2021Quote: ... and purified using Monarch RNA cleanup kits (NEB).
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bioRxiv - Bioengineering 2020Quote: A Q5 sitedirected mutagenesis kit (New England Biolabs) was used to insert a hexahistidine tag at the N-terminal of the SP141 gene of the P22GFP expression plasmid via standard PCR protocols ...
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bioRxiv - Biochemistry 2021Quote: ... via NEBuilder HiFi DNA Assembly Cloning Kit (NEB). Vector template was amplified using primers oEcoli-C_48 and 49 ...
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bioRxiv - Biochemistry 2020Quote: The Q5 Site directed mutagenesis kit (NEB, E0554S) was used to make the following mutations All mutant constructs were verified by Sanger sequencing.