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Citations for New England Biolabs :
1 - 50 of 8872 citations for RPA kit reagent since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... The RT-RPA was performed at 42°C for 30 min by combining M-MuLV-RT (NEB #M0253L) with TwistAmp Basic (TwistDx #TABAS03KIT) ...
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bioRxiv - Biochemistry 2022Quote: ... EvaGreen DNA dye was replaced with RPA fluorescent probes (DENV2-CAP-probe at 0.12 μM) with 100 U Exonuclease III (NEB), keeping all other reagents same ...
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bioRxiv - Cell Biology 2023Quote: ... and 200 μM each of dATP/dCTP/dTTP at 21°C for 10 min and an additional 15 min incubation with 25 nM recombinant human RPA (a gift from Sarah W. Cai) and 0.03 U/μl T4 DNA polymerase (NEB, #M0203) at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... The USER™ (uracil-specific excision reagent) Friendly Cloning Kit (NEB) was used for all the cloning procedures ...
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bioRxiv - Cell Biology 2022Quote: ... The standard procedure and reagents from the Q5 Site-Directed Mutagenesis Kit (NEB) were used to insert mutations ...
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bioRxiv - Molecular Biology 2022Quote: ... or HiScribe™ T7 mRNA Kit with CleanCap® Reagent AG (NEB E2080S) as per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: The reagents used were as follows: Q5® Site-Directed Mutagenesis Kit (NEB), Gibson Assembly® Master Mix (NEB) ...
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bioRxiv - Immunology 2023Quote: ... These amplicons were used as templates for in vitro transcription using the HiScribe T7 mRNA Kit with CleanCap® Reagent AG kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesized with RevertAid First Strand cDNA Synthesis Kit (ThermoScientific) reverse transcription kit and PCR was carried out using OneTaq® DNA Polymerase reagents (NEB) using conditions previously detailed and oligonucleotides described in Suppl ...
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bioRxiv - Cell Biology 2022Quote: ... Reagents and general procedures from the Gibson Assembly Cloning Kit from New England Biolabs (NEB) were used ...
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bioRxiv - Evolutionary Biology 2023Quote: ... mRNA was transcribed with HiScribe T7 mRNA Kit with CleanCap Reagent AG (New England Biolabs) according to manufacturer’s protocol with 1 µg of amplified template ...
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bioRxiv - Microbiology 2023Quote: ... and then were incubated with linearized pGL3-promoter vector and the Gibson Assembly Kit reagents (NEB). Reactions were then transformed into competent Top10 E ...
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bioRxiv - Genomics 2023Quote: ... Two libraries were prepared for Nanopore MinION sequencing using the Ligation Sequencing kit (SQK-LSK108, ONT, Oxford, UK) and NEBnext DNA Repair kit reagents (NEB, Ipswich, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: Library preparation was modified from protocols.IO (dx.doi.org/10.17504/protocols.io.bagaibse) utilizing reagents from the NEB Next II DNA Ultra Kit (New England Biolabs #E7645S) and Unique Combinatorial Dual index kit (New England Biolabs #E6442S, ...
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bioRxiv - Evolutionary Biology 2023Quote: ... complemented by reagents from the NEBNext Ultra II library prep kit (New England Biolabs, Ipswich, Massachusetts, U.S.A.), and then sequenced at a MinION platform (Oxford Nanopore Technologies ...
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bioRxiv - Microbiology 2019Quote: ... then protocol and reagents from NEBNext® Ultra™ DNA Library Prep Kit for Illumina (New England Biolabs) were used to end repair ...
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bioRxiv - Microbiology 2019Quote: ... protocols and reagents from NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (New England Biolabs) were used for end repair ...
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bioRxiv - Genomics 2020Quote: ... Protocols and reagents from NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (New England Biolabs) were used for end repair ...
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bioRxiv - Molecular Biology 2019Quote: All reagents from NEB except where noted otherwise ...
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bioRxiv - Microbiology 2019Quote: ... Antisense strand DNA was digested in the samples using the Uracil-Specific Excision Reagent Enzyme Kit (New England Biolabs), followed by a PCR to amplify the cDNA ...
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bioRxiv - Cancer Biology 2022Quote: ChIP sequencing libraries were prepared using NEBNext® Ultra™ II DNA Library Prep Kit reagents (NEB, Ipswitch, MA) according to the procedure recommended by the manufacturer ...
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bioRxiv - Microbiology 2022Quote: ... Antisense strand DNA was digested in the samples using the Uracil-Specific Excision Reagent Enzyme Kit (New England Biolabs), followed by a PCR to amplify the cDNA ...
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bioRxiv - Cancer Biology 2023Quote: ChIP sequencing libraries were prepared using NEBNext® Ultra™ II DNA Library Prep Kit reagents (NEB, Ipswitch, MA) according to the procedure recommended by the manufacturer ...
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bioRxiv - Synthetic Biology 2022Quote: ... molecular biology reagents from NEB and Life Technologies and general laboratory reagents from Corning ...
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bioRxiv - Cancer Biology 2023Quote: ... using Gibson Assembly reagents (NEB). Select elements were removed from the backbone (such as rtTA3 ...
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bioRxiv - Molecular Biology 2021Quote: ... Resulting cell pellets were washed in 1x PBS and resuspended in 1x DNA/RNA protection reagent (Monarch Total RNA Miniprep Kit, NEB). Subsequently ...
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bioRxiv - Synthetic Biology 2021Quote: ... cell pellets were washed 1× with PBS and collected in 1X DNA/RNA protection reagent (Monarch Total RNA Miniprep Kit, NEB). Cells were lysed and total RNA was isolated following the mammalian cell protocol inlcuding on-column DNase I treatment ...
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bioRxiv - Microbiology 2020Quote: ... RT-qPCR reactions were carried out using LightCycler 96 instrument and following reagents: Luna Universal One-Step RT-qPCR Kit (New England Biolabs, NEB) for hCoV-229E and hCoV-OC43 ...
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bioRxiv - Immunology 2022Quote: RNA was extracted from IgG1 stimulated B cells 72 hour post-stimulation using TRIzol reagent (Fisher) and cDNA was prepared using the Protoscript II kit (NEB). Germline transcription was analyzed using quantitative PCR with SYBR Green (Roche ...
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bioRxiv - Microbiology 2022Quote: ... 12 µl template DNA was supplemented with the required reagents from the NEBNext Ultra II End Repair/dA Tailing kit (E7546S, NEB) and was first incubated at 20°C for 5 minutes and then at 65°C for 5 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were then mixed with 350 μl 1× RNA protection reagent and RNA was purified using a Monarch total RNA extraction kit (NEB) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... The elution product was directly used for second-strand synthesis using reagents from the NEBNext Ultra II Directional RNA Library Prep Kit (NEB E7760). 8 μL of Second Strand Synthesis Reaction Buffer ...
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bioRxiv - Microbiology 2019Quote: ... We added 1 mL of RAD reagent from the RAD002 kit and 0.2mL of Blunt TA ligase (New England Biolabs, MA, USA) and the reaction was incubated for 5 minutes at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... RT-qPCR reactions were carried out using LightCycler 96 instrument and following reagents: Luna Universal One-Step RT-qPCR Kit (New England Biolabs, NEB) for hCoV-229E and hCoV-OC43 ...
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bioRxiv - Microbiology 2019Quote: ... plus 280 nM each primer and the standard reagents in the Phusion High-Fidelity PCR Kit (New England BioLabs, Ipswich, MA). Samples were then cycled under the following qPCR conditions ...
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bioRxiv - Bioengineering 2023Quote: mRNA was synthesized through in vitro transcription using the HiScribe T7 mRNA Kit with CleanCap Reagent AG (NEB, China, Cat: E2080S). The templates for in vitro transcription were linearized plasmids ...
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bioRxiv - Bioengineering 2024Quote: ... The bacterial pellet was then resuspended in 1X DNA/RNA protection reagent supplied with the Monarch Total RNA Miniprep Kit (NEB #T2010) (New England BioLabs, USA) and subjected to mechanical lysis using the FastPrep-24™ 5G bead beating grinder system (MP Biomedicals Germany GmbH) ...
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bioRxiv - Bioengineering 2024Quote: ... The bacterial pellet was then resuspended in 1X DNA/RNA protection reagent supplied with the Monarch Total RNA Miniprep Kit (NEB #T2010) (New England BioLabs ...
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bioRxiv - Genetics 2021Quote: ... PCR reagents were purchased from NEB, and all primers were synthesized by Integrated DNA Technologies (IDT).
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bioRxiv - Microbiology 2024Quote: Molecular biology reagents were from NEB and chemical reagents were from Sigma unless otherwise noted ...
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bioRxiv - Microbiology 2020Quote: ... plus 280 nM for each primer and the standard reagents in the Phusion High-Fidelity PCR Kit (New England BioLabs, Ipswich, MA). Samples were then cycled under the following qPCR conditions ...
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bioRxiv - Bioengineering 2022Quote: ... These linearized templates were used to direct in vitro transcription (IVT) of mRNAs using the HiScribe™T7 mRNA Kit with CleanCap® Reagent AG (NEB). Cy3-UTP and Cy5-UTP were used replacing 25% of standard UTP in some experiments to fluorescently label the mRNA transcripts ...
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bioRxiv - Cell Biology 2023Quote: The 2NSP23 nanobody mRNA was prepared by in vitro translation of a DNA molecule of using the HiScribe™ T7 mRNA Kit with CleanCap® Reagent AG (New England BioLabs). The obtained mRNA has the following sequence and includes a 5’-CAP and a poly-A tail ...
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bioRxiv - Microbiology 2023Quote: Libraries were prepared using the NEBNext® DNA Library Prep Reagent Set for Illumina® kit (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2019Quote: ... the culture sample was diluted with the DNA/RNA protection reagent of the Monarch Total RNA Miniprep Kit (New England Biolabs, Ipswich, MA, USA), followed mechanical lysis with ZR BashingBead™ Lysis Tube (0.5mm ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... RNA extraction was performed using TRI reagent (Cosmo Bio Co., Ltd., Tokyo, Japan) and the Monarch Total RNA Miniprep Kit (New England BioLabs Inc., Ipswich, MA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... and inserted with NEBuilder reagents (New England Biolabs) into pBACc3 opened with BamHI and PstI ...
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bioRxiv - Biochemistry 2020Quote: ... Reagents for the KLD reactions came from NEB: T4 PNK (M0236S) ...
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bioRxiv - Genomics 2020Quote: ... NEBNext DNA Ultra II Reagents (NEB, Ipswich, MA) were used according to the manufacturer’s instructions for end repair ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was extracted using TRI reagent (BioLabs) according to the manufacturer specifications ...