Labshake search
Citations for New England Biolabs :
251 - 300 of 8872 citations for RPA kit reagent since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: Site-Directed Mutagenesis Kit (NEB E0554). Following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... Monarch® RNA Cleanup Kit (NEB) was used to purify RNAs ...
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bioRxiv - Synthetic Biology 2023Quote: Monarch DNA Gel Extraction kit (NEB), for purifying PCR products products prior to assembly (Note ...
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bioRxiv - Molecular Biology 2023Quote: ... or NEB Miniprep Kits (NEB, T1010).
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bioRxiv - Molecular Biology 2023Quote: ... The NEBNext rRNA depletion kit (NEB) was used to deplete ribosomal RNA ...
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bioRxiv - Immunology 2024Quote: ... The NEBNextII kit (New England Biolabs) was used to prepare the sequencing library ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Q5 Site-Directed Mutagenesis Kit (NEB) was used according to manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: ... The Monarch RNA Cleanup Kit (NEB) was used to purify the resulting RNA ...
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bioRxiv - Molecular Biology 2024Quote: ... Q5 site directed mutagenesis kit (NEB) was used to generate various SARS-CoV-2 FSE conformation stabilizing constructs and other length dependent constructs and their sequences were confirmed through sanger sequencing (Eurofins genomics) ...
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bioRxiv - Cell Biology 2024Quote: ... using the HiFi Assembly kit (NEB). NEBuilder Assembly Tool 2.0 was used to design RRM1-2 primers with 21-bp overlap with the destination vector ...
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bioRxiv - Microbiology 2020Quote: ... cleaned and used to prepare dual-indexed sequencing libraries using NEBNext Ultra library prep reagents (New England Biolabs Inc., Ipswich, MA) and barcoding indices synthesized in the CDC Biotechnology Core Facility ...
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bioRxiv - Microbiology 2022Quote: Total RNA from 24 h bacterial culture in LB without NaCl was isolated from bacteria using TRIzol reagent (HiMedia) and cDNA synthesized using ProtoScript® II Reverse Transcriptase (NEB). qRT-PCR was performed using PowerUp™ SYBR™ Green Master Mix (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... per the manufacturer’s directions and then subjected to qRT-PCR using a single step reaction using Luna reagent (NEB, IPswhich MA). The primers and probes (5’-6FAM and Iowa Black Quencher ...
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bioRxiv - Microbiology 2020Quote: ... The resulting mRNA is used for library construction for RNA-seq using NEBNext mRNA Library Prep Reagent set for Illumina (NEB, USA). Briefly ...
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bioRxiv - Immunology 2020Quote: ... For genotyping hair samples were incubated in 250 μL of Direct PCR reagent and 20 mg/mL Proteinase K (New England Biolabs, P8102) for 1 h at 55°C ...
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bioRxiv - Neuroscience 2022Quote: ... sequencing libraries were constructed using the NEBNext ChIP-seq Library Prep Reagent Set for Illumina and a NextSeq 500 Illumina sequencer (New England BioLabs Inc.).
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bioRxiv - Genomics 2022Quote: ... Looped adapter sequences were opened by removal of uracil from hairpin structures by adding 3 units of USER enzyme (Uracil-Specific Excision Reagent) (NEB, M5505S) and incubation at 37 °C for 15 min ...
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bioRxiv - Microbiology 2023Quote: ... Each sample was amplified in triplicate 25 μl reactions consisting of primers at final concentrations of 0.2 μM and 1X PCR reagent (Q5® Hot Start High-Fidelity 2X Master Mix, New England BioLabs, UK). Triplicate PCR reactions for each sample were then pooled and purified using Agencourt® AMPure® XP beads (#A63881 ...
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bioRxiv - Molecular Biology 2023Quote: The cells were infected with ZIKV and DENV2 and lysed using the Luna Cell Ready Lysis reagent (New England Biolabs, UK). Quantification of viral RNA was performed from cell lysates using the virus specific primers and their levels were normalized to mRNA of GAPDH (Table 1) ...
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bioRxiv - Genetics 2023Quote: ... 2.5 µl of homogenate (1/100th homogenate volume) was removed and kept on ice in a total of 300 µl 1X Monarch DNA/RNA Protection Reagent (NEB, T2011L) as the input sample ...
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bioRxiv - Microbiology 2023Quote: ... antibiotic resistance cassette and 2 flanking regions were combined in equimolar amounts and mixed with 2 x HiFi reagent (NEB, UK) and incubated at 50°C for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... The libraries were then prepared by digesting the hairpin structures on both primer adaptors using Uracil-Specific Excision Reagent (USER) enzyme (NEB; M5505) and a following PCR amplification for 16 cycles with Truseq barcoded primers ...
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bioRxiv - Systems Biology 2024Quote: ... we directly engineered the pre-barcoded pSL51 and pre-barcoded pSL737 plasmid pools using PCR and HiFi in vitro recombinational assembly (NEB reagents) to generate a construct that should express a single methionine start-codon fused to the Gateway attL2/attR2 “scar” sequence (YPAFLYKVV) ...
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bioRxiv - Molecular Biology 2023Quote: ... Amplicon was subsequently purified with column DNA Clean-up kit (Monarch PCR & DNA Cleanup Kit, # T1030L, NEB) and mixed with double-digested Nhe1/ BamH1 and gel-purified target pCW57.1 vector in a molecular ratio of 1:2 (50 ng Vector ...
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bioRxiv - Microbiology 2023Quote: ... using the kit Luna® Universal qPCR Master Mix Kit (New England Biolabs, Ipswich, MA, United States). PCRs were conducted in a total volume of 20 µl containing 10 μL of Luna Universal qPCR Mix ...
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bioRxiv - Cancer Biology 2021Quote: ... Kras cDNA was amplified in PCR reaction using the corresponding primers (Reagents) and Phusion Hot Start Flex polymerase (New England Biolabs, Ipswich, MA). cDNA fragments were purified with NucleoSpin gel and PCR clean-up columns (Macherey-Nagel ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the resulting cell pellet was resuspended in 1 mL of RNA Protection Buffer (1× NEB DNA/RNA Protection Reagent, 1% (w/v) polyvinylpyrrolidone-40 ...
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bioRxiv - Developmental Biology 2020Quote: ... Sequencing libraries were prepared from 10 μg of the 3C library by sonication to an average size of 200 ~ 300 bp and indexed using NEBnext reagents (New England Biolabs, Lot # 0031607), according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and paired-end libraries with ~300 bp inserts prepared using NEBNext DNA Library Prep Reagent Set for Illumina (New England Biolabs no. E6000L). Each library’s concentration and quality was analysed with an Agilent 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Molecular Biology 2019Quote: ... 2x 1-5 µg of DNA were adaptor ligated and indexed using the NEBNext DNA library Prep Reagent Set (New England Biolabs: E6040S/L) and NEBNext Multiplex Oligos for Illumina Primer sets 1 (New England ...
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bioRxiv - Genetics 2019Quote: ... 2× 1-5 µg of DNA were adaptor ligated and indexed using the NEBNext DNA library Prep Reagent Set (New England Biolabs: E6040S/L) and NEBNext Multiplex Oligos for Illumina Primer sets 1 (New England ...
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bioRxiv - Microbiology 2020Quote: ... polymerases and Gibson assembly mix reagents were used to manipulate DNA fragments and plasmids according to manufacturer instructions (New England Biolabs and Promega).
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bioRxiv - Evolutionary Biology 2021Quote: ... but here notably we corrected for post-mortem-damage by adding the uracil-specific excision reagent (USER™, New England Biolabs Inc.) during the blunt end repair step (for further detail see Supplementary Methods) ...
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bioRxiv - Bioengineering 2022Quote: ... and 28.7 uL of water were combined to form a 50 uL reaction (Phusion PCR reagents were purchased from New England Biolabs, Ipswich, MA). The thermocycling protocol proceeded first with an incubation at 98°C for 30 seconds ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were then purified using the DNA cleanup column kit (the Monarch PCR & DNA cleanup kit from NEB).
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bioRxiv - Biochemistry 2020Quote: ... Samples were then purified using the DNA cleanup column kit (the Monarch PCR & DNA cleanup kit from NEB). The specificity of AlkB repair reaction was confirmed using an inactive AlkB control reaction in which Fe2+ ...
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bioRxiv - Neuroscience 2022Quote: ... Ribo-Zero Gold (Human/Mouse/Rat) Kit (Illumina; NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)(E6350, NEB); NEXTflex™ Small RNA Sequencing Kit v3 (PerkinElmer ...
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bioRxiv - Genetics 2022Quote: ... or FLAG (amino acid sequence: DYKDDDDK) with a kit (Gibson assembly kit from New England Biolabs, catalogue # E5510S). The different combinations were cloned into pJFRC7-20XUAS-IVS-mCD8::GFP (Addgene # 26220 ...
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bioRxiv - Genomics 2022Quote: ... Library preparation was performed using the NEBNext Ultra DNA Library Prep Kit (kit number E7370L, New England Biolabs) and 8 cycles of PCR ...
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bioRxiv - Microbiology 2023Quote: ... DNA was isolated from blood (QiaAmp DNA Mini Blood Kit, Qiagen; or Monarch Genomic DNA purification kit, NEB) or from perfused organs homogenized (Fisher Bead Mill 4 ...
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bioRxiv - Developmental Biology 2023Quote: ... NEB One Taq RT-PCR kit (One Taq® RT-PCR Kit, New England Biolabs INC, Frankfurt, Germany) was used for cDNA synthesis ...
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bioRxiv - Synthetic Biology 2021Quote: ... or Monarch PCR & DNA Cleanup Kit (NEB) according to the manufacturer’s protocols ...
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bioRxiv - Developmental Biology 2021Quote: NEBNext rRNA Depletion Kit (NEB, Cat E6310S) was used to deplete ribosomal RNA according to the manufacture’s instruction ...
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bioRxiv - Neuroscience 2022Quote: ... The HiScribe Quick T7 kit (NEB, E2050S) was then used for in vitro transcription ...
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bioRxiv - Molecular Biology 2020Quote: ... a quick ligation kit (New England Biolabs) was used ...
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bioRxiv - Molecular Biology 2021Quote: ... Q5 Site-Directed Mutagenesis Kit (NEB E0554S) and restriction cloning were used to generate partial constructs ...
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bioRxiv - Synthetic Biology 2019Quote: ... Q5 site directed mutagenesis kit from NEB was used for cloning the inserts inferiors at 100 bps ...
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bioRxiv - Microbiology 2019Quote: ... and end repaired (Quick Blunting Kit, NEB). Transposon junctions were amplified from gDNA by PCR ...
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bioRxiv - Developmental Biology 2019Quote: ... and Monarch Total RNA miniprep kit (NEB). RNA concentration was measured using Nanodrop (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... Phusion high fidelity DNA polymerase kit (NEB) was used for the PCR amplification and optimized with DMSO and formamide ...