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Citations for New England Biolabs :
101 - 150 of 1875 citations for Potassium Bromate 90 95% Chemical Purity 18O3 98% 100 Ug Ml In 18O Water since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... 2 ug of DNA was treated with uracil DNA glycosylase (UDG, New England Biolabs, Inc.) for 60 minutes at 37°C with gentle shaking ...
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bioRxiv - Molecular Biology 2023Quote: ... Four ug of fragmented gDNA was digested with 20 U of RNase H (NEB #M0297S) at 37°C for 6 hours to serve as a negative control ...
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bioRxiv - Cell Biology 2023Quote: ... 100 µg/mL cycloheximide) supplemented with 0.1 U/µL murine RNase Inhibitor (NEB), and passed through a G25 needle 25 times ...
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bioRxiv - Molecular Biology 2020Quote: ... and protein purity and concentration were analyzed along with T7 RNAP (New England Biolabs, #M0251S) by 10% SDS-PAGE gel electrophoresis stained with Coomassie blue (Bio-rad).
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bioRxiv - Genomics 2022Quote: ... 3 ug of input DNA was dephosphorylated with Quick CIP (New England Biolabs, cat no M0508). Following enzyme inactivation with alkaline phosphatase ...
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bioRxiv - Biochemistry 2024Quote: ... Reactions were further quenched by the addition of 0.2 ug of RNAse A (New England Biolabs) and incubation at 37 °C for 20 min ...
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bioRxiv - Cell Biology 2021Quote: ... pH 7.8) and 100 μg/ml of proteinase K (New England Biolabs, Ipswich, MA) for 15 min at room temperature ...
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bioRxiv - Genomics 2022Quote: ... proximity ligated sample was treated with 100 μL Proteinase K (NEB P8107S-800U/ml), 100μL 10% SDS (Invitrogen Cat no ...
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bioRxiv - Molecular Biology 2019Quote: m7G-capped RNA substrates 90 to 1400 nucleotides long were generated by T7 RNA polymerase (NEB) transcription of different DNA templates generated from an FLuc plasmid ...
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bioRxiv - Systems Biology 2019Quote: ... Biotinylation was performed in a 90 µl reaction with 6 U of T4 ssRNA Ligase (NEB), 4 µl Biotinylated Cytidine (Thermo Fisher ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and the P1-adaptors and Sbf I-HF (New England Biolabs)-digested DNAs for 90 min at 37°C were ligated by T4 ligase (New England Biolabs). After DNA pooling ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were treated with RNAse (100 μg/ml) at room temperature for 15 min and then with proteinase K (825 μg/ml, NEB™, P8107S) for 1 hour at 65°C ...
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bioRxiv - Synthetic Biology 2021Quote: ... annealed by temperature decrease from 95 °C to 4 °C and phosphorylated using T4 Polynucleotide Kinase (NEB, M0201) according to manufactures’ protocol ...
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A small molecule reveals role of insulin receptor-insulin like growth factor-1 receptor heterodimersbioRxiv - Cell Biology 2021Quote: ... cDNA samples were diluted in Nuclease-free water (NEB) before use in qPCR reactions ...
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bioRxiv - Microbiology 2019Quote: ... then 100 μL of 4 mg/mL p-nitrophenyl phosphate (New England Biolabs, MA, USA) was added and the mixture was vortexed again ...
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bioRxiv - Microbiology 2023Quote: ... coli strains following the chemical transformation protocol from NEB for functional characterization ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 µl genomic DNA (input 90 ng) from naïve hESCs were digested with LpnPI (New England Biolabs) generating 32 bp fragments around the fully methylated recognition site containing a CpG ...
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bioRxiv - Cell Biology 2024Quote: ... cells were treated for 90 min with a 1:150 dilution of RNase III (New England Biolabs) in low-salt buffer (50 mM Tris-HCl pH 7.6 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were resuspended in spheroblast buffer (1.2 M sorbitol, 0.1 M potassium phosphate, 20 mM vanadyl ribonuclease complex [NEB S1402S], 20 μM beta-mercaptoethanol) and digested with 0.002% 100T zymolyase (US Biological Z1005 ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 ug of RNA was reverse-transcribed using the WarmStart® RTx reverse transcriptase (New England BioLabs, M0380L) and random primer mix (New England BioLabs ...
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bioRxiv - Developmental Biology 2023Quote: ... mRNA was isolated from 2.5 ug of total RNA using NEBnext Poly (A) magnetic isolation module (E7490S, NEB). The library was prepared as per the user’s manual using the NEBNext Ultra II Directional RNA library Prep Kit for Illumina (E7760S ...
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bioRxiv - Neuroscience 2020Quote: ... The holder allowed immersion of the cochlea and the middle ear in oxygenated (95 % O2, 5 % CO2) cell culture medium (Minimum Essential Medium with Earle’s balanced salts, SH30244.FS Nordic Biolabs). The bone of the bulla was removed gently with bone cutters which exposed the middle ear and the basal turn of the cochlea ...
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bioRxiv - Cancer Biology 2023Quote: ... were then denatured at 95°C for 5 min and cooled at room temperature to form heteroduplexes using NEBuffer2.1 1x (NEB). The T7 endonuclease I (NEB ...
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bioRxiv - Biophysics 2019Quote: ... Purity and molecular weight were confirmed by staining with a SNAP-tag-reactive benzylguanine-Alexa 647 dye (NEB) and running the proteins on a 4-20% NuPAGE Bis-Tris gel ...
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bioRxiv - Genomics 2021Quote: ... The purity of DNA for PacBio sequencing was also verified with a EcoRI (New England BioLabs, Ipswich, MA) restriction digest and sized via electrophoresis on a 1% agarose gel with a clamped homogeneous electric field (CHEF ...
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bioRxiv - Biochemistry 2019Quote: ... transferred at 300 mA for 90 minutes and probed overnight with α-MBP (1:10000, New England BioLabs) or α-ORC (1:1000 ...
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bioRxiv - Systems Biology 2024Quote: ... PCR products (90 bp) were spin-purified and 300 ng was digested with BsaI-HF-v2 (NEB R3733) in a 100 µl reaction for 2 h at 37°C (no heat kill ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 50 ul PCR reactions were set up with approximately 1 ug cDNA and Phusion High-Fidelity DNA Polymerase (NEB) was used ...
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bioRxiv - Biochemistry 2021Quote: ... RNA integrity was assessed by performing 1% TBE agarose gel electrophoresis with samples that had been boiled for 95°C for 5 min in RNA loading dye (New England Biolabs). Genomic DNA was eliminated by incubating 2 μg of RNA with 2 U of RQ1 RNase-free DNase I (Promega ...
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bioRxiv - Systems Biology 2020Quote: ... One microgram of purified RNA was fragmented at 95 °C for 7 min in 1X T4 RNA Ligase buffer (NEB) with an equal volume of 2X alkaline fragmentation buffer (0.6 volumes of 100 mM Na2CO3 plus 4.4 volumes of 100 mM NaHCO3) ...
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bioRxiv - Biochemistry 2021Quote: ... coli or Expi293™ cells underwent denaturation at 95 °C for 10 min in glycoprotein denaturing buffer (New England Biolabs) followed by immediate cooling on ice for 10 s ...
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bioRxiv - Plant Biology 2021Quote: ... Supplementary Table 1) were hybridized by slow cooling from 95-25°C and then phosphorylated using T4 Polynucleotide Kinase (NEB). The digested plasmid and the hybridized oligonucleotides were ligated using T4 Ligase (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... 95°C/15 s and annealing/extension: 65°C/5 min) of PCR using Q5 high-fidelity DNA polymerase (NEB). Amplicons were purified using 1× AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Genomics 2021Quote: ... 95°C/15 s and annealing/extension: 65°C/5 min) of PCR using Q5 high-fidelity DNA polymerase (NEB). Amplicons were purified using 1× AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Bioengineering 2022Quote: ... pGRNA-galK was linearized by PCR using primers 95 and 391 and assembled with the sacB-fragment by Gibson assembly (New England Biolabs).
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bioRxiv - Molecular Biology 2022Quote: ... the RNA was fragmented at 95°C for 5 min by using a Next Magnesium RNA Fragmentation Module (New England Biolabs), and cleaned up by using a MinElute PCR Purification Kit (Qiagen) ...
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bioRxiv - Genetics 2023Quote: Genomic DNA was extracted from individual embryos by heat shock denaturation in 50 mM NaOH (95°C for 20 minutes) and used for PCR by using Q5 High-Fidelity Taq Polymerase (New England Biolabs). The presence of specific amplicons was tested by running the PCR product on 1% agarose gel ...
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bioRxiv - Molecular Biology 2023Quote: ... Second-strand DNA synthesis was done by ligating 10 µM of a blunt-end duplex comprised of a 32-bp Illumina R1-3’SpC3 and 5’-phosphorylated R1R-3’SpC3 DNA (pre-annealed by incubating at 95°C for 3 min and slowly cooling to 25°C) using a Quick ligase kit (New England Biolabs) according to manufacturer’s protocol followed by clean-up as described above ...
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bioRxiv - Cell Biology 2023Quote: ... A de novo sequence was formed by annealing oligo 3 and 4 over a 30 min temperature gradient from 95°C to 25°C and integrated via T4 ligase (New England Biolabs). pZE13d-C_PfCen3 and _PfCen4 were generated by digesting pZE13d-C with BamHI-HF and SalI-HF and integrating codon optimized PfCen3 and PfCen4 encoding sequences PCR amplified via primer pairs P34+35 and P36+37 ...
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bioRxiv - Molecular Biology 2024Quote: ... Table S4) were hybridised by slow cooling down from 95-25°C and then phosphorylated using the T4 Polynucleotide Kinase (NEB). The digested plasmid and the hybridised oligonucleotides were ligated using the T4 ligase (NEB ...
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bioRxiv - Biophysics 2020Quote: ... concentrated in a 100 kDa concentration device (Amicon) to ~1.5 mg/ml and incubated with EndoHF (NEB) at a volume to volume ratio of 1:20 for 2 hours to remove the N-linked glycan of EWI-FΔIg1-5 ...
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bioRxiv - Molecular Biology 2023Quote: ... pull-down was performed with 100 μl pre-blocked (NETS buffer with 4 mg/ml BSA (NEB) and 2 mg/ml tRNA (SigmaAldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... 1 mM EDTA pH 8.0 (Roth) and 1:100 Proteinase K (New England Biolabs 20 mg/ml)) in a 2 ml Eppendorf tube for at least 24 hours at RT in the dark ...
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bioRxiv - Microbiology 2023Quote: ... Desialylation of Calu-3 cells was achieved by incubating cells grown in a 96 well plate or 24-well plate or 6 well plate with 100 U/mL α2-3,6,8,9 neuraminidase A (P0722L, NEB) in 10% FCS media for 3 h at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... Pre-tRNA was resuspended in nuclease-free water (New England Biolabs) at 100 μM stock concentration ...
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bioRxiv - Synthetic Biology 2021Quote: ... SARS-CoV-2 RNA was replaced with nuclease free water (NEB) for the assay without the target RNA ...
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bioRxiv - Biophysics 2019Quote: ... 70 µL Millipore water and 10 µL T4 DNA ligase (NEB) were added to the hybridization reaction and incubated for 60 min at 20 °C.
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bioRxiv - Bioengineering 2022Quote: ... we used 10 uL of nuclease-free water (BioLabs, cat# 52904B1500S) as an elution buffer to obtain the extracted RNAs from the membrane.
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bioRxiv - Microbiology 2023Quote: ... sterile water and then treated with DNase I (New England Biolabs) for 60 mins at 37°C ...
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bioRxiv - Genomics 2019Quote: ... fragments compatible with Illumina NGS were generated digesting the products of rolling circle amplification with BtsαI (55 °C, 90 min, New England Biolabs) and SalI-HF (37 °C ...