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Citations for New England Biolabs :
351 - 400 of 1875 citations for Potassium Bromate 90 95% Chemical Purity 18O3 98% 100 Ug Ml In 18O Water since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Equivalent amounts of gDNA from the different cells lines was digested in a 37°C water bath for four hours with HpaII and MspI isochizomer enzymes (NEB, Inc.) Following digestion ...
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bioRxiv - Neuroscience 2022Quote: ... The non-15N 4R tau used for small-scale chemical in vitro crosslinking was phosphorylated using cAMP-dependent Protein Kinase (PKA, catalytic subunit, New England Biolabs #P6000S) according to manufacturer guidelines (25uL reaction at 30°C for 2 hours with 200 μM ATP ...
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bioRxiv - Microbiology 2020Quote: ... The flies were homogenised in 100 μl of TE-buffer pH 8 containing 1% Triton X-100 and 1% Proteinase K (NEB, P8107S). Homogenates were incubated for 3 h at 55°C followed by a 10 min incubation step at 95°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then divided into two aliquots and treated with and without 100 U M.CviPI GpC methyltransferase (100 U/million cells; New England Biolabs, M0227B-HI) supplemented with fresh 160 μM S-adenosyl-L-methionine for 15 min at 37ºC ...
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bioRxiv - Physiology 2023Quote: ... 5 flies were sorted into sterile microcentrifuge tubes and homogenised in 200 µl lysis buffer (1 X TE, 1 % Triton X-100, 1/100 proteinase K (NEB, P8107S)) using a mechanical pestle ...
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bioRxiv - Molecular Biology 2023Quote: ... Oligos for side 1 and 2 were dimerized separately by mixing 9 μl of OligoA at 100 μM with 9 μl of OligoB at 100 μM and 2 μl of 10x DNA Ligase Buffer (NEB, M0202S) and heating to 95 °C for 5 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... the crude extract containing 100 μg (in 100 μL volume) of proteins was treated with 400 units of λ-PPase (#P0753; New England Biolabs) for 60 min at 30 °C ...
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bioRxiv - Biochemistry 2021Quote: ... Nb35–His (10 μg/mL) and apyrase (25 mU/mL, NEB); the suspension was incubated for 1 h at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Nb35–His (10 µg/ml) and apyrase (25 mU/ml, NEB). The suspension was incubated for 1 h at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... eluted in 20 μl of water and PCR amplified using 25 μl NEB Next High-Fidelity 2x PCR Master Mix (NEB, #M0541 L), 2.5 μl of P5_BRB primer (5 μM ...
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bioRxiv - Immunology 2022Quote: ... sorted cells were lysed in 6.5uL of mild lysis buffer (10mM NaCl, 10mM Tris-HCL, pH 7.5; 0.5% NP-40, 0.25uL of 40 U/uL RNase-inhibitor [NEB M0314] and nuclease free water) for 30’ at 4oC ...
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bioRxiv - Genetics 2023Quote: ... One microliter of T-tailed DNA adaptors diluted to 1.5 µM in water was added to 5 µl of amplicons diluted to 1/10 in water and 5 µl of 2X Blunt/TA Ligase Master Mix (New England Biolabs, Herts, UK) and incubated 30 min at 25°C for ligation ...
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bioRxiv - Bioengineering 2022Quote: ... and 28.7 uL of water were combined to form a 50 uL reaction (Phusion PCR reagents were purchased from New England Biolabs, Ipswich, MA). The thermocycling protocol proceeded first with an incubation at 98°C for 30 seconds ...
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bioRxiv - Microbiology 2023Quote: ... DNA was ligated for 4 h in a water bath at 16 °C (30 Weiss Units of T4 DNA Ligase, M0202 New England BioLabs® Inc). 300 μg of Proteinase K was used to reverse the cross-linking overnight at 65°C ...
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bioRxiv - Cancer Biology 2023Quote: ... DNAse-treated RNA in 5uL water was used as input to the NEB Ultra II directional library-preparation kit (New England BioLabs, MA, USA). If the concentration was less than this ...
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bioRxiv - Microbiology 2023Quote: ... and lysed with 2× concentrated lysis buffer (0.25% Triton X-100, 50 mM KCL, 100 mM Tris-HCl pH 7.4, 40% glycerol, 1/50 volume of RNase inhibitor; NEB, Cat. No. M0314S) at room temperature for 10 min ...
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bioRxiv - Biophysics 2024Quote: ... First the 5’ triphosphate of RNA was converted into a 5’ monophosphate by incubating 100 µg RNA with 100 units of RNA 5’ Pyrophosphohydrolase (NEB, Ipswich MA) at 37°C for 1 hour within a 100 µl reaction volume ...
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bioRxiv - Cell Biology 2019Quote: Chemical induction of ER stress in zebrafish embryos was accomplished by using a final concentration of .5mM DTT (NEB #B1034S; Invitrogen Y00147), 1uM Thapsigargin (Sigma #T9033) ...
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bioRxiv - Microbiology 2023Quote: ... A 1 ml aliquot of cells was incubated with 1 μl M13KO7 (1011 plaque-forming units per ml, pfu/ml) (NEB) for 10 min on ice to allow attachment ...
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bioRxiv - Synthetic Biology 2021Quote: ... and/or 100 U SP6 RNA Polymerase (NEB #M0207L), 15μM of DFHBI-1T and/or Malachite Green dye ...
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bioRxiv - Microbiology 2020Quote: ... DNA was digested with 100 units of MboI (NEB) and ligated in the nucleus followed by in situ Hi-C protocol [42] ...
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bioRxiv - Pathology 2020Quote: ... gDNA (100 ng) was digested with TaqaI (NEB R0149) at 65⁰C for 2h followed by MspI (NEB R0106 ...
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bioRxiv - Microbiology 2021Quote: ... or 100 nM TMR substrate for SNAPtag (S9105S, NEB), for 30 min anaerobically ...
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bioRxiv - Genomics 2021Quote: ... Chromatin was digested with 100 units of MboI (NEB) at 37°C for 2 hours and subsequently incubated at 62°C for 20 minutes to inactivate the MboI ...
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bioRxiv - Cancer Biology 2021Quote: ... chitin magnetic beads (100 μL, E8036S, New England Biolabs) were added to the reaction mixture.
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bioRxiv - Cancer Biology 2022Quote: ... 100 U exonuclease III (both from New England Biolabs), and incubation for 60 min at 37°C and 20 min at 80°C.
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bioRxiv - Neuroscience 2022Quote: ... 100 ul of RNAse Inhibitor (New England BioLabs M0314L) was added to 250 ul of Imaging Buffer Activator (PN 203000015 ...
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bioRxiv - Neuroscience 2022Quote: ... 100 uL T4 DNA Ligase (New England Biolabs; M0202L), 100 uL BSA 10 mg/mL ...
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bioRxiv - Molecular Biology 2022Quote: ... microcapsules were treated with 100 U Exonuclease I (NEB) for 15 minutes at 37 °C ...
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bioRxiv - Neuroscience 2022Quote: ... 100 ul of RNAse Inhibitor (New England BioLabs M0314L) was added to 250 ul of Imaging Buffer Activator (PN 203000015 ...
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bioRxiv - Neuroscience 2022Quote: ... 100 ul of RNAse Inhibitor (New England BioLabs M0314L) was added to 250 ul of Imaging Buffer Activator (PN 203000015 ...
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bioRxiv - Neuroscience 2023Quote: ... 100 U of M-MLV Reverse Transcriptase (NEB; M0253S), M-MLV RT buffer (NEB ...
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bioRxiv - Cancer Biology 2023Quote: ... 100 μM RSH 5194 and 1x UDG buffer (NEB)48 ...
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bioRxiv - Bioengineering 2023Quote: 100 μM EnGen® Lba Cas12a (New England Biolabs) was diluted as needed in the supplied Cas12a diluent ...
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bioRxiv - Genomics 2023Quote: ... resuspended in 100 μl DpnII buffer (New England Biolabs), and pelleted again ...
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bioRxiv - Biophysics 2023Quote: ... 100 μL of TEV protease (New England Biolabs, #P8112S) was added ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was digested overnight with 100 units MboI (NEB) restriction enzyme then the ends filled using dTTP ...
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bioRxiv - Genomics 2023Quote: ... plus 100 U of the MboI enzyme (NEB #R0147), then incubating for ≥2 hours at 37 °C in a Thermomixer at 900 rpm ...
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bioRxiv - Molecular Biology 2023Quote: ... or RNase If (1:100 diluted, New England Biolabs) in buffer 3 (100 mM NaCl ...
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bioRxiv - Genomics 2023Quote: ... 100 μL of RNAse Inhibitor (New England BioLabs M0314L) was added to 250 μL of Imaging Buffer Activator (PN 203000015 ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.5 mM DTT) with 100 units of MNase (NEB), incubated at 37℃ for 5 min ...
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bioRxiv - Molecular Biology 2021Quote: ... 4 ml ScaI (BioLabs), followed by 3 h of incubation with a fresh portion ...
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bioRxiv - Molecular Biology 2020Quote: ... BSA (2mg/ml, NEB), dextran sulfate (10% ...
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bioRxiv - Biochemistry 2022Quote: ... 0.1mg/mL BSA (NEB), 0.1 mg/mL Baker’s Yeast tRNA (Sigma R5636) ...
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bioRxiv - Microbiology 2023Quote: ... 20μl/ml DNase (NEB) and RNase cocktail (Amersham) ...
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bioRxiv - Bioengineering 2024Quote: ... – 400 U/mL (NEB); Jack bean hexosaminidase (JBH ...
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bioRxiv - Systems Biology 2020Quote: ... reaction components were added for second strand synthesis (70 μL water, 20 μL NEB second strand buffer, 10 μL NEB second strand enzyme). Second strand synthesis was carried out as described and the double-stranded cDNA was used as input for tagmentation ...
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bioRxiv - Genomics 2019Quote: ... The reaction was topped up to 200μL with DEPC treated water and a phenol chloroform extract performed (as before except the pellet was resuspended in 17.5μL DEPC treated water. The SR RT primer (NEB small RNA kit for Illumina) was hybridized to the 3’ adapter and the 5’ adapter ligated by adding 2μL T4 RNA ligase buffer (NEB) ...
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bioRxiv - Genomics 2021Quote: ... 5 μl of 100 ng/μl cDNA diluted from the previous step was combined with 2 μl block mix and 2 μl nuclease free water (NEB, catalog no. AM9937), then the cDNA-block oligo mix was incubated on a thermocycler under the following condition to allow block oligo mix to bind to 5’ and 3’ end of the cDNA molecule ...
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bioRxiv - Developmental Biology 2023Quote: ... along with the guide RNA at a concentration of 300 ng/μl were mixed in molecular grade water with Cas9 buffer (NEB, Cat No. M0386S). Embryos were injected 6 hrs after egg laying for arm and 3hrs for frizzled4 and wnt7 ...