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Citations for New England Biolabs :
101 - 150 of 10000+ citations for Mouse Two pore calcium channel protein 2 Tpcn2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... the PURExpress® In Vitro Protein Synthesis Kit (New England Biolabs, E6800L) was used as described in the instructions with minor modifications ...
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bioRxiv - Microbiology 2023Quote: ... the PURExpress® In Vitro Protein Synthesis Kit (New England Biolabs, E6800L) was used as described previously (31) ...
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bioRxiv - Biochemistry 2021Quote: ... two guide RNAs and the Cas9 enzyme (S. pyogenes, NEB) were diluted to a final concentration of 20 ng/µl each in injection buffer ...
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bioRxiv - Microbiology 2019Quote: ... The two fragments were joined together using Gibson reaction (NEB) and the resulting plasmid was transformed into E ...
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bioRxiv - Molecular Biology 2019Quote: ... two unique BamHI restriction sites were introduced by mutagenesis (NEB’s plasmid mutation kit ...
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bioRxiv - Genomics 2021Quote: Synthesised oligos underwent two consecutive runs of Q5 (NEB, M0491) low-cycle PCR using two different sets of primers (see Supp Table 4) ...
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bioRxiv - Plant Biology 2021Quote: ... the two cassettes were assembled into pMDC123 vector by NEB Gibson Assembly to generate the nucleus-mcherry-pt-CFP marker construct ...
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bioRxiv - Microbiology 2024Quote: ... Two µl of SNAP-Cell Block (New England Biolabs #S9106S) were added to strain 2C4.3 WT ...
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bioRxiv - Biochemistry 2023Quote: ... Bound protein was detected by western blot using mouse anti-MBP diluted 1:10,000 (New England Biolabs), fluorescent secondary antibodies (Li-Cor Biosciences) ...
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bioRxiv - Biochemistry 2022Quote: ... The two pairs of PCR products were ligated together by in vitro homologous recombination using a Gibson assembly cloning kit (NEB, Boston, MA, USA), respectively ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Two DNA sequencing libraries were prepared from two wAlbB S mosquitoes using the NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina (New England Biolabs, E7805L) and NEBNext® Multiplex Oligos for Illumina® (New England Biolabs ...
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bioRxiv - Neuroscience 2022Quote: ... or the NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)(E6350, NEB) (replicates 4-5 and no salt treatment ...
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bioRxiv - Molecular Biology 2022Quote: ... we used NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) (NEB #E7405), NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB #E7765 ...
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bioRxiv - Genetics 2023Quote: ... using the NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) (NEB E7405) or the NEBNext Poly(A ...
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bioRxiv - Synthetic Biology 2023Quote: The cell-free protein synthesis (CFPS) reactions were carried out using the PURExpress In Vitro Protein Synthesis Kit (New England Biolabs Inc) in 384-well plates (Thermofisher NUNC ...
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bioRxiv - Microbiology 2023Quote: RdnE proteins were produced using the New England Biolabs PURExpress In Vitro Protein Synthesis Kit (New England BioLabs Inc., Ipswich MA). Template DNA contained the rdnE gene and required elements specified by the PURExpress kit ...
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bioRxiv - Cell Biology 2023Quote: ... a PCR fragment containing the complete Nv-osk coding sequence was synthesized for protein expression with PURExpress® In Vitro Protein Synthesis Kit (New England Biolabs) using manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: 1 μg of N protein was dissolved in 36 μL H218O and 2 μL 10x glycobuffer (NEB). To this solution 2 μL PNGase F was added and the reaction mixture was incubated at 37 °C for 16 h ...
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bioRxiv - Molecular Biology 2022Quote: ... Removal of fucose was performed using α1-2,4,5,6 fucosidase O (New England Biolabs, 2 U/μg protein), and α1-3,4 fucosidase (New England Biolabs ...
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bioRxiv - Physiology 2019Quote: 40 μg of membrane protein were denaturated by adding 2 μl of denaturation buffer (New England Biolabs), for a final volume of 20 μl ...
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bioRxiv - Neuroscience 2020Quote: ... PCR fragments were inserted into linearized NaV channel expression plasmids by Gibson Assembly using NEBuilder HiFi Assembly Mix according to the manufacturer’s protocol (New England BioLabs). Introns were inserted after c.2946 (exon 15/16 junction ...
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bioRxiv - Cancer Biology 2022Quote: ... DHFR control plasmid from PURExpress In Vitro Protein Synthesis Kit (New England Biolabs) was used as backbone for subsequent ligations ...
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bioRxiv - Microbiology 2019Quote: ... (2) DSBs blunting with NEB’s Quick Blunting Kit (NEB, cat. number: E1201); (3 ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 µM IllLigBio adapter pre-adenylated using a 5’ adenylation kit (NEB), 20-200 nM pppUGAAUG hexamer standard ...
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bioRxiv - Microbiology 2021Quote: ... was utilized using SARS-CoV-2 specific primers[43] or SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit (New England Biolabs), which became available in assays after September 15 ...
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bioRxiv - Microbiology 2022Quote: ... human NINL isoform 2 (NCBI accession NM_001318226.2) and the NINL isoform 2 mutant (Q231R) were mutagenized using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs). The plasmids encoding 3C proteases (coxsackievirus B3 (CVB3 ...
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bioRxiv - Cancer Biology 2023Quote: ... and ligated to hairpin adaptor 2 (ENDseq-adaptor-2, 5′-Phos-GATCGGAAGAGCACACGTCUUUUUUUUAGACGTGTGCTCTTCCGATC*T-3′ [*phosphorothioate bond]) (Quick ligation kit, NEB). To prepare libraries for sequencing ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The two fragments were assembled using HI-FI assembly (NEB E5520S). To construct the Prcan-1-R1∷mCherry and Prcan-1-R2∷mCherry plasmids ...
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bioRxiv - Cell Biology 2021Quote: ... These two oligos were ligated by incubation with T4 ligase (NEB; 1U/μl T4 DNA ligase and 1× T4 DNA ligation buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... Two different concentrations (80 and 160 Gel Units) of MNase (NEB) were used ...
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bioRxiv - Microbiology 2021Quote: ... the two fragments were generated using Phusion PCR (New England Biolabs) by amplifying the TurboGFP open reading frame from the vector pGIPZ (Thermo Scientific Open Biosystems ...
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bioRxiv - Microbiology 2022Quote: ... We used two high fidelity PCR systems—Q5 (NEB cat. M0492), and Platinum SuperFi II (Invitrogen cat ...
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bioRxiv - Microbiology 2022Quote: pJJW101 was constructed using two stepwise NEBuilder HiFi assemblies (NEB, E5520S) followed by restriction and ligation ...
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Circularization of rv0678 for genotypic bedaquiline resistance testing of Mycobacterium tuberculosisbioRxiv - Molecular Biology 2022Quote: Two micrograms of amplicon was digested with Lambda Exonuclease (NEB, USA) in a 30ul reaction at 37°C for 30 minutes according to the manufacturer’s instructions ...
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FACS-Sortable Picoreactors for Ultra High-throughput Screening of Catalysts in Biphasic EnvironmentsbioRxiv - Bioengineering 2024Quote: ... the two inner solutions were comprised of 1) PURExpress reagents (NEB) or 2 ...
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bioRxiv - Genetics 2020Quote: ... following rRNA depletion using a NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB).
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bioRxiv - Microbiology 2021Quote: ... Standard curve was prepared using SARS-CoV-2 Positive Control plasmid containing full nucleocapsid protein (N gene) (NEB) and used to quantify copies of N gene in organoid samples ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were expressed and purified from 2 to 4 liters of Escherichia coli NiCo21(DE3) (New England Biolabs) as previously described (24) ...
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bioRxiv - Molecular Biology 2021Quote: ... Mutant protein expression vectors were generated using the Q5 Mutagenesis Kit (New England BioLabs) and proteins overexpressed and purified the same as wild-type.
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bioRxiv - Biochemistry 2023Quote: ... Assays were set-up using PURExpress in vitro protein synthesis kit (New England Biolabs) with a cloned full-length DHFR template ...
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bioRxiv - Molecular Biology 2019Quote: Libraries were prepared with NEBNEXT rRNA Depletion kit (human/mouse/rat) and NEBNEXT Ultra II Directional RNA Library Prep kit for Illumina (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... the two PCR products were purified and then digested with AarI (NEB) enzyme at 37 C for 5 hrs ...
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bioRxiv - Synthetic Biology 2021Quote: A two-part Golden Gate assembly reaction with BsaI (New England Biolabs) was used to insert the PCR-amplified oligo pool into the PCR-amplified backbone ...
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bioRxiv - Developmental Biology 2020Quote: ... two washes were performed in NEBNext dA-Tailing Reaction Buffer (NEB, B6059S), followed by A-tailing (Klenow 3’-> 5’ exo- ...
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bioRxiv - Microbiology 2021Quote: ... The two oligos were then phosphorylated using T4 polynucleotide kinase (NEB, USA) at 37°C for 1 hr ...
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bioRxiv - Genomics 2022Quote: ... two adaptor ligations using T4 RNA Ligase 1 (catalog no. M0204; NEB) and reverse transcription using SuperScript III Reverse Transcriptase (catalog no ...
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bioRxiv - Neuroscience 2023Quote: ... These two fragments were digested by EcoRI (New England Biolabs, Cat # R0101) and then linked together using T4 DNA ligase (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... and M.Fnn25V for two hours at 37 °C in CutSmart buffer (NEB) containing 160 μM S-adenosylmethionine (SAM ...
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bioRxiv - Biophysics 2023Quote: ... These two fragments where ligated together using T4 DNA ligase (M0202, NEB). The resulting plasmid can have the T7A1 promoter in two different orientations ...
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bioRxiv - Biochemistry 2023Quote: ... and IMut5SB constructs were created by a two-fragment assembly system (NEB), replacing WT INCENP1–58 sequence with the corresponding gBlock Gene Fragments (IDT) ...