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Citations for New England Biolabs :
451 - 500 of 10000+ citations for Mouse Two pore calcium channel protein 2 Tpcn2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... Cas9 protein (New England Biolabs) and PAGE purified ssHDR template (Integrated DNA Technologies ...
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bioRxiv - Developmental Biology 2022Quote: ... Cas9 protein (NEB, Ipswich, MA), and ssODN (Integrated DNA Technologies ...
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bioRxiv - Bioengineering 2024Quote: ... coli Protein Synthesis System (NEB) [48] at 2 x the reaction scale ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein Deglycosylation Mix II (NEB) was added and further incubated for 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... PCR amplified products were generated and two-or three-piece Gibson assembly was performed per manufacture’s instruction (New England Biolabs)
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bioRxiv - Genetics 2021Quote: ... the linear construct and genomic carrier DNA of RSA635 were incubated for two hours with PstI (New England BioLabs). The construct (60 ng/µl ...
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Genome Assembly of the Roundjaw Bonefish (Albula glossodonta), a Vulnerable Circumtropical SportfishbioRxiv - Genomics 2021Quote: ... We carried out double digests of 150 – 200 ng total DNA per sample using the two enzymes in the manufacturer’s supplied buffer (New England Biolabs) for 8 hours at 37°C ...
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bioRxiv - Immunology 2021Quote: ... sgRNA designed by online design tool (http://chopchop.cbu.uib.no/) and two DNA oligo annealing by T4 Polynucleotide Kinase (NEB, Cat#M0201L) and cloned into a Bbs1-digested U6-sgRNA-PGK-Puro-BFP retrovirus vector by T4 DNA ligation ...
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bioRxiv - Microbiology 2019Quote: ... The two PCR products were then assembled into the vector using the NEBuilder HiFi DNA Assembly Master Mix (NEB).
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bioRxiv - Genomics 2022Quote: The library for high-throughput sequencing was prepared in a two-step PCR (Q5 high-fidelity DNA polymerase, NEB). In PCR1 ...
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bioRxiv - Biophysics 2022Quote: ... The two primers are annealed and the single strand overhangs are filled in using One-Taq DNA polymerase (NEB). These filled-in primers are then amplified by PCR with One-Taq DNA polymerase (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... Two separate 8-µl aliquots of beads derived from each strain were treated with 1 µl lambda phosphatase (NEB) in 10 µl reactions containing ...
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bioRxiv - Genomics 2022Quote: ... Two nested single-tail adapter/tag (STAT)-PCR [29] were performed with LongAmp Hot Start Taq polymerase (NEB, M0533S) using adaptor binding P5_1 and P5_2 primers and corresponding insert primer pairs (see Supplementary Table 2) ...
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bioRxiv - Cell Biology 2022Quote: ... The two fragments were fused using the in-fusion enzyme into a pM retroviral vector cut with EcoRI (NEB) and SalI (NEB ...
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bioRxiv - Genomics 2019Quote: Each DNA sample was split in two and separately nicked with either 30 U of Nb.BspQI (New England Biolabs) in 1× Buffer 3 (Bionano Genomics ...
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bioRxiv - Genomics 2020Quote: The library was prepared for high-throughput sequencing in two rounds of PCR (Q5 High-Fidelity DNA Polymerase, NEB). In PCR1 ...
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bioRxiv - Biochemistry 2022Quote: ... and the poly(A) RNA was isolated with two rounds of selection using oligo-(dT)25 beads (NEB, S1419S). The purified poly(A ...
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bioRxiv - Evolutionary Biology 2022Quote: 75ng of genomic DNA were used for PCR amplification in two 50µl reactions with 25µl 2xQ5 mix (NEB M0492), 2.5µM of each forward (FOS ...
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bioRxiv - Microbiology 2022Quote: ... was generated by two fragment Gibson assembly using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, Ipswich, MA). The plasmid pACYC184 (pACYCev ...
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bioRxiv - Neuroscience 2022Quote: ... The last two PCR rounds were performed using NEBNext® Ultra™ II Q5® Master Mix (NEB #M0544). Samples were purified using AMPure XP beads (Beckman Coulter #A63880) ...
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bioRxiv - Molecular Biology 2022Quote: Sequencing libraries were prepared from 52.5 μg gDNA using two-step PCR strategy with NEBNext Ultra II Q5 Polymerase (NEB). First the gRNA regions were enriched from the genome ...
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bioRxiv - Synthetic Biology 2023Quote: ... Each library was split in two and transformed separately into 5-alpha cells (NEB, 3.5uL DNA in 35uL cells). Plasmid library isolation ...
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bioRxiv - Plant Biology 2023Quote: ... Two micrograms of total RNA were used for first-strand cDNA synthesis using AMV reverse transcriptase (New England Biolabs). A quantitative real-time PCR (RT-qPCR ...
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bioRxiv - Neuroscience 2023Quote: ... These two amplicons were incubated with SfoI digested pET23a-His-TEV plasmid in a HiFi Assembly reaction (NEB, E2621S) to generate a plasmid where HaloTag and SpyCatcher3 is linked with a GSGESGSG linker sequence.
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bioRxiv - Developmental Biology 2024Quote: ... Two guide RNAs targeting chordin and Three guide RNAs targeting tyrosinase were transcribed using T7 RNA polymerase (NEB #M0251s) from DNA templates made from the following primers for chordin ...
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bioRxiv - Molecular Biology 2023Quote: ... the circular fragments were amplified by two rounds of polymerase chain reaction (PCR) with Q5-High Fidelity polymerase (NEB). Both PCR rounds begin with an initial denaturation at 98 °C for 30 s ...
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bioRxiv - Cancer Biology 2023Quote: ... Two pairs of primers were used to perform PCR amplification with Q5 High-Fidelity 2X Master Mix (NEB, #M0492S). All validation primers are listed in Supplementary Table 3 ...
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bioRxiv - Bioengineering 2023Quote: ... The two obtained PCR fragments were assembled by NEBuilder® HiFi DNA Assembly Master Mix (E2621, New England BioLabs) into a pET28a backbone generated by PCR with primers 13 and 14 ...
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bioRxiv - Biochemistry 2024Quote: Sequencing libraries for each sorted bin were generated by two-step PCR using a Phusion® DNA Polymerase (NEB) kit ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 - 400 ng of PCR fragments were used as template DNA to synthesize analytic amounts of DNA deaminases using PURExpress In Vitro Protein Synthesis kit (NEB, Ipswich, MA) following manufacturer’s recommendations.
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bioRxiv - Molecular Biology 2019Quote: ... and 2 μl of DNase I (2 U/μl, New England Biolabs) to 30 μl RNA product ...
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bioRxiv - Biochemistry 2023Quote: ... and 2′O-methylated using Vaccinia 2′O Methyltransferase (New England Biolabs). The IRES-containing mRNAs were uncapped and polyadenylated.
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bioRxiv - Developmental Biology 2023Quote: Rehydrated embryos were blocked for 2 hs in 2% BSA (B9000, NEB) in PBS with 0.3% Triton X-100 (T9284 Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... Yields of viral RNA were quantified by real-time qPCR by using SARS-CoV-2 specific primers targeting the E gene with the Luna®Universal One-Step RT-qPCR Kit (New England Biolabs) in a LightCycler 480 thermocycler (Roche ...
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bioRxiv - Biophysics 2021Quote: ... We isolated the total RNA from 2×107 cells using a Monarch Total RNA Miniprep Kit (T2010, New England Biolabs, MA) as described by the manufacturer with an additional 30-minute ...
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bioRxiv - Microbiology 2022Quote: ... Bands from 200 to 400 bp were selected by extraction from a 2% agarose gel using a Monarch DNA Gel Extraction Kit (NEB#T1020L). Purified adaptor-DNA fragments were amplified by Phusion polymerase (NEB#M0530 ...
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bioRxiv - Molecular Biology 2019Quote: ... 150 ng of double stranded gBlock® template or 2 µg of plasmid template was transcribed using the HiScribe T7 High Yield RNA Synthesis Kit (New England BioLabs®). At the end of the reaction ...
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bioRxiv - Biochemistry 2020Quote: cDNA was reverse-transcribed from donor 1 and donor 2 RNA samples using the ProtoScript II first strand cDNA synthesis kit (NEB #E6560) with oligo dT priming ...
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bioRxiv - Genetics 2020Quote: Libraries were prepared from 2 ng of DNA using the NEBNext Ultra II FS DNA library prep kit (New England Biolabs, USA) following the manufacturer’s instructions ...
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bioRxiv - Pathology 2019Quote: ... protocol using the PCR Barcoding Expansion 1-12 (EXP-PBC001) kit (ONT) and LongAmp Taq 2× Master Mix (New England Biolabs, MA) with the following thermocycling conditions ...
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bioRxiv - Immunology 2019Quote: ... and libraries were prepared from 2 ng of total RNA using the NEBNext low input kit (New England Biolabs, Hitchen, U.K.). Libraries were assessed for correct size distribution on the Agilent 2200 TapeStation and quantified by Qubit DNA High Sensitivity assay (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... equal amount of DNA (∼2 ng) was used as an input for NEB Ultra II DNA library prep kits (NEB #E7645). Number of cycles for amplification of adapter ligated libraries were estimated by the qPCR before final amplification to avoid any bias arising due to PCR amplification and indexing (NEB #E7350) ...
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bioRxiv - Cell Biology 2022Quote: ... An IFT144 construct lacking the N-terminal β-propeller domain (residues 2–349 inclusive; IFT144ΔNFLAG) was made using the Q5® Site-Directed Mutagenesis Kit (NEB).
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bioRxiv - Genetics 2022Quote: ... 500 ng of purified PCR products (D2500, Gel Extraction Kit, OMEGA, USA) were denatured and reannealed in NEB buffer 2 (M0302S, NEB, USA): 95 ℃ ...
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bioRxiv - Microbiology 2022Quote: RNA-free PXO99A genomic DNAs (0.2 μg) were used to construct the DNA libraries using a NEBNext Ultra DNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... The mutant and wild-type target RNA were subsequently amplified using either the NEB Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit (NEB E3019), Luna Probe One-Step RT-qPCR 4X Mix with UDG (NEB M3019 ...
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bioRxiv - Developmental Biology 2023Quote: ... was combined with either MEP-1 or Mi-2 PCR-amplified coding sequences in a Gibson assembly reaction using NEBuilder® HiFi DNA Assembly kit (NEB) following manufacturers protocols ...
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bioRxiv - Molecular Biology 2023Quote: ... All mRNAs were transcribed at 30°C for 2 hrs using the HiScribe T7 High Yield RNA Synthesis Kit (NEB # E2040S) and were co-transcriptionally capped (8:1 cap analog to GTP for ∼90% capping efficiency ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Libraries were created using 1000ng of total RNA with the NEBNext Ultra 2 Directional RNA Library Kit following the Poly(A) mRNA Magnetic Isolation module (NEB #E7490). Libraries were sent to Novogene for Illumina Hi-Seq ...
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bioRxiv - Genetics 2023Quote: ... Index primers set 1 and 2 from the NEBNext Multiplex Oligos for Illumina kit (New England Biolabs, E7335S/L E7500S/L) were incorporated using Herculase II Fusion Polymerase Kit (Agilent ...