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Citations for New England Biolabs :
1301 - 1350 of 2067 citations for 5 Sulfosalicylaldehyde sodium salt since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... the 5′ linker (containing four random nucleotides) was ligated to the small RNAs using T4 RNA ligase (NEB) overnight at 16°C ...
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bioRxiv - Microbiology 2019Quote: ... the PCR products were purified using Monarch® PCR & DNA Cleanup Kit (5 μg; New England Biolabs GmbH). For the second PCR ...
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bioRxiv - Synthetic Biology 2020Quote: ... was achieved by PCR of the relevant design using primers F_RiboJ_Rem and R_RiboJ_Rem (Supplementary Table 5) and subsequent circularization by standard Golden Gate assembly (New England Biolabs, E1601S) to create the plasmids pVB003 and pVB004 ...
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bioRxiv - Biophysics 2020Quote: ... Fluorescence labelling was done by incubating with 5 nmol SNAP-Surface Alexa Fluor 488 (#S9129S, New England Biolabs) for 1 hour at 4 °C ...
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bioRxiv - Neuroscience 2019Quote: ... 5 µg of Intein-RapGAP-SIPA1L2 (470-838) and Intein-Caldendrin were immobilized on chitin resin (NE BioLabs). Resin was washed with TBS buffer (150 mM NaCl ...
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bioRxiv - Genomics 2020Quote: ... RNA was resuspended in 5 μl of water and the 3′ ends dephosphorylated with PNK (New England BioLabs) in MES buffer (100 mM MES-NaOH ...
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bioRxiv - Neuroscience 2019Quote: ... Ten microliters of PCR products were digested with Nsi1 (3 hours with 5 units of Nsi1 enzyme (NEB)) and then ...
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bioRxiv - Plant Biology 2020Quote: ... were amplified from genomic DNA using primers in Supplemental Table 5 and Taq Phusion polymerase (New England BioLabs), cloned in pGEM-t Easy (Promega) ...
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bioRxiv - Microbiology 2020Quote: ... 15 μg of DNA-depleted RNA was incubated with 12.5 units of RNA 5’ pyrophosphohydrolase (RppH) (#M0356, NEB) in a 50 μl reaction for 1 h at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... the cap-1 structure was added to the 5’ end using the Vaccinia capping enzyme (New England Biolabs) and Vaccinia 2’-O-methyltransferase (New England Biolabs) ...
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bioRxiv - Genomics 2020Quote: ... Chromatin within intact nuclei was then digested for 5 minutes with micrococcal nuclease (NEB #M0247, 500U/1M cells) prior to nuclei lysis by Covaris sonication ...
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bioRxiv - Biochemistry 2021Quote: ... gDNA was quantified by Nanodrop and a fraction of DNA was treated with 5 U RNase H (NEB) for 1h at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... Residual template switch adaptor was removed by incubation with 5 U of uracil DNA glycosylase (New England BioLabs) for 40 min at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... and was then installed with 5’cap (Vaccinia Capping System, NEB, USA; Cap 2’-O-methyltransferase, NEB, USA) and 3’ Poly(A ...
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bioRxiv - Microbiology 2019Quote: ... 20 μg of total RNA were ligated with 50 pmol 3′adaptor Linker (5′-rAppCTGTAGGCACCATCAAT–NH2-3′; NEB) through a 16 h incubation at 16°C with 20 U T4 RNA ligase (Ambion) ...
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bioRxiv - Biochemistry 2022Quote: 3’-PUA-DNA was generated by incubation of 1 μM 5’-FAM-labeled AP-DNA (FAM_U_35) with 20 U EndoIII/Nth (NEB) in Buffer B pH 7.0 at 37 °C for 60 m ...
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bioRxiv - Microbiology 2022Quote: ... 2.5µL of 10µM MBTUni-12 primer + 2.5µL of 10µM MBTUni-13 primer (5’-ACGCGTGATCAGTAGAAACAAGG-3’) + 10µL 5x HF Phusion Buffer + 1µL 10mM dNTPs mix (NEB #N0447S) + 0.5µL Phusion Polymerase + 28.5µL of Nuclease-free water (Ambion ...
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bioRxiv - Biochemistry 2021Quote: ... 5 µL of PCR product from the inner primer amplification was cleaned used 0.5 µL Exonuclease I (NEB) and 1 µL rSAP (NEB) ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were incubated on ice for 5 minutes then immediately treated with appropriate concentration of RNAse If(NEB) for 3 mins at 37C at 1200RPM ...
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bioRxiv - Cell Biology 2021Quote: ... 5 µg antibody (Table. S3) and 50 µl protein A magnetic beads (New England Biolabs, Cat. No. S1425S) were added to the supernatant and incubate overnight at 4 degree Celsius ...
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bioRxiv - Molecular Biology 2019Quote: ... samples were then radiolabeled on bead with 50ul of the following mix: 5 ul 10X PNK buffer (NEB), 1 ul RNaseOUT ...
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bioRxiv - Bioengineering 2019Quote: ... 1.2 µg of the sheared DNA was blunted and 5’-phosphorylated using the NEBNext End Repair Module (NEB), and 3’-A-tailed with NEBNext dA-Tailing Module (NEB ...
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bioRxiv - Molecular Biology 2019Quote: ... The RNA templates were degraded by incubating reactions with 5 units of RNase H (M0297, NEW ENGLAND BIOLABS) and 1.0 µl of RNase cocktail enzyme mix (AM2286 ...
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bioRxiv - Microbiology 2019Quote: The DNA oligo i116 that served as a 3’ adapter was adenylated using 5’ DNA Adenylation Kit (NEB), purified by ethanol precipitation as above and diluted to 10 μM with nuclease-free water.
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bioRxiv - Cell Biology 2019Quote: ... 5-10 µg of total protein (9 µl of lysate) were treated with Endo Hf (New England Biolabs) for 1 h at 37°C ...
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bioRxiv - Cancer Biology 2019Quote: ... UltraScript 2.0 (PB30.31-10, lot #PB130614-01-5, PCR Biosystems, Wayne PA) and WarmStart RTx (M0380L, lot #0061705, NEB) by each manufacturer’s protocol with minor modifications ...
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bioRxiv - Molecular Biology 2020Quote: ... Sample was pelleted at 2500xg for 5 minutes and the pellet was washed with 1x Cutsmart buffer (NEB) and pelleted again ...
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The absence of C-5 DNA methylation in Leishmania donovani allows DNA enrichment from complex samplesbioRxiv - Molecular Biology 2020Quote: ... and cloned between 300 bp of PCR amplified DNA fragments of the LdBPK_250018100.1 5’ and 3’ UTR using NEBuilder (NEB) inside pUC19 for construct amplification in E ...
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bioRxiv - Immunology 2020Quote: ... and the assembled plasmid pCO1 was transformed into NEB® 5-alpha competent Escherichia coli (High Efficiency) (NEB) according to manufacturer’s instructions (see Supplementary Figure 1 for plasmid maps) ...
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bioRxiv - Molecular Biology 2020Quote: ... 2.5 μl 10X ligation buffer, 0.8 μl 100% DMSO, 2.5 μl T4 RNA ligase I – high concentration [NEB] ...
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bioRxiv - Molecular Biology 2020Quote: ... The cDNA reaction mix was therefore compensated by adding 0.5 μl 5’Ligation Reaction Buffer 10x (NEB-kit) and 1.75 μl nuclease-free H2O.
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bioRxiv - Cancer Biology 2019Quote: ... and then resuspended in sorting buffer (PBS with 0.5% BSA, 2.5 mM MgCl2, 0.5 mM CaCl2, 1 µg/mL DAPI and 100 U/mL DNaseI, NEB) and incubated for 30 minutes at room temperature before sorting ...
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Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... Pre-adenylated DNA linker(DNA oligo purchase from IDT was adenylated using 5′ DNA Adenylation Kit (NEB,E2610S)) was ligated to the 3’-end of RNA in a 20 µl reaction (25% PEG8000 ...
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bioRxiv - Molecular Biology 2021Quote: ... by AgeI and EcoRI digestion and ligation of 5’-phosphorylated DNA duplexes using T4 DNA ligase (NEB, #M0202). Target plasmids expressing 0 and 480 CTG repeats in the context of DMPK exons 11-15 (DMPKS and DT480 ...
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bioRxiv - Molecular Biology 2021Quote: ... R: 5’-ccgggaaaaactgaaaaaccattggcacgacaggtttcccgac-3’ from the pKAM555 vector) was inserted at the ClaI site using HiFi assembly (NEB). For the intTEL0 strain creation the pUC19LG plasmid (lacking the PstI(blunted)-BclI fragment of the HARS36 sequence ...
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bioRxiv - Biophysics 2021Quote: ... The phosphorylated 5’-ends were subjected to RNA ligation by 20 units of T4 RNA ligase 1 (NEB) in the presence of 10 mM DNA/RNA rP5_RND oligo (Supplemental Table 3 ...
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bioRxiv - Molecular Biology 2021Quote: ... 1.5 μl 10 mM dNTPs, 0.5 μl 10 μM NEB Universal PCR primer, 0.5 μl 10 μM NEB index primer ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA (5 μg) was ligated to the RNA 3’ adaptor using T4 RNA Ligase 2 - truncated (NEB), in the presence of RNase Inhibitor (NEB) ...
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bioRxiv - Immunology 2020Quote: ... TAP reaction was performed in a total volume of 25 μL using 5 μL of Q5 polymerase (NEB), 5 μL of GC Enhancer (NEB) ...
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bioRxiv - Molecular Biology 2022Quote: ... 1.5 μl 10 mM dNTPs, 0.5 μl 10 μM NEB Universal PCR primer, 0.5 μl 10 μM NEB index primer ...
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bioRxiv - Molecular Biology 2022Quote: ... 1.5 μl 10 mM dNTPs, 0.5 μl 10 μM NEB Universal PCR primer, 0.5 μl 10 μM NEB index primer ...
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bioRxiv - Microbiology 2022Quote: ... Either 3-5 µL of Color Prestained Protein Standard-Broad Range (11-245 kDa) (New England Biolabs, P7712) or 1-3 µL of PageRuler Prestained Protein Ladder (10-180 kDa ...
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bioRxiv - Microbiology 2022Quote: ... RNA was resuspended in 5 μl of water and the 3′ ends dephosphorylated with PNK (New England BioLabs) in MES buffer (100 mM MES-NaOH ...
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bioRxiv - Microbiology 2022Quote: ... poly(A) RNA (2-5 ng) was converted to cDNA using the Protoscript II kit (New England Biolabs) and a supplied mix of random hexamer and d(T)23VN primers ...
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bioRxiv - Microbiology 2022Quote: ... Escherichia coli NEB® 5-alpha was used for plasmid assembly and cloning following the manufacturer’s instructions (NEB). Transformed E ...
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bioRxiv - Molecular Biology 2023Quote: ... and decapped with 10 U of T4 PNK +/− 40 nmol of ATP and 5 U of RppH (NEB). After each step ...
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bioRxiv - Genetics 2022Quote: ... The second strand cDNA synthesis was performed using Klenow fragment 3’-5’ exo (New England Biolabs Inc, USA), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... 50 uL of Quick Ligation Buffer and 5 uL of Quick T4 Ligase (NEB Quick Ligation Kit, M2200S). The ligation reaction was incubated for 10 minutes at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... CaMKII substrates were added to the 5’ end of cpGFP by site-directed mutagenesis (KDL Enzyme Mix, NEB). deadCaMKART6A was generated by site-directed mutagenesis of pcDNA3.1:CMV-CaMKAR ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 µg total RNA was subjected to poly(A) mRNA isolation according to the manufacturer’s protocols (NEB, #E7490). Then ...