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Citations for New England Biolabs :
1151 - 1200 of 2067 citations for 5 Sulfosalicylaldehyde sodium salt since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: The unc-5 locus was amplified for sequencing by PCR using OneTaq 2x Master Mix (NEB) with primers DLO1081 (5’-TCTTTTCAGGCTTTGGCACTG-3’ ...
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bioRxiv - Cell Biology 2019Quote: ... 40 nM down primer and 5 μM dNTP in 17 μl 1 x CutSmart buffer (NEB). The RNA and primers were incubated at a temperature gradient ...
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bioRxiv - Molecular Biology 2019Quote: ... Thawed cells were resuspended in 5 ml of 1X NEB2 restriction enzyme buffer (New England Biolabs) and poured into a pre-chilled mortar containing liquid N2 ...
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bioRxiv - Molecular Biology 2021Quote: ... a 5’ RACE RNA adapter was ligated to total Aag2 RNA with T4 RNA ligase (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... 5 µg of the PCR product was subjected to Fragmentase® treatment (New England Biolab, NEB) until a smear of fragments was detected around 400-500pb by agarose gel electrophoresis ...
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bioRxiv - Biophysics 2020Quote: ... and were 5’ end-radiolabeled with [Ɣ-32P]-ATP using 10 U T4 polynucleotide kinase (NEB). Radiolabeled transcripts were purified on polyacrylamide gel (6% acrylamide:bisacrylamide 19:1 ...
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bioRxiv - Genetics 2019Quote: ... Thawed cells were resuspended in 5 ml of 1X NEB2 restriction enzyme buffer (New England Biolabs) and poured into a pre-chilled mortar containing liquid N2 ...
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bioRxiv - Biochemistry 2021Quote: Capping of RNA with the 5’-m7G cap was achieved using the Vaccinia Capping System (NEB), according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... the 5′ triphosphate was first removed by treatment with Quick CIP (New England BioLabs, manufacturer’s instructions). The reaction was then supplemented with 5 mM DTT and the same concentrations of T4 polynucleotide kinase (New England BioLabs ...
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bioRxiv - Molecular Biology 2021Quote: ... the reaction product was combined with 20 μl digestion mix containing 5 μl ExoI (NEB, M0293S), 5 μl HinFI (NEB ...
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bioRxiv - Developmental Biology 2020Quote: ... DNA fragments were end-repaired and A-Tailed using Klenow fragment (3’-5-exo-) (NEB, M0212L), the DNA fragments were ligated to illumina adaptors (Illumina ...
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bioRxiv - Cell Biology 2021Quote: ... For each sample 250 ng of DNA was digested with 5 Units of RNase H (NEB) as control ...
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bioRxiv - Genomics 2021Quote: ... biotin was removed from the unligated ends by adding 5 µl of T4 DNA polymerase (NEB) to 5 ug of DNA ...
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bioRxiv - Genetics 2021Quote: ... Singlet and duplet pools were A-tailed using using Klenow HC 3’→5’ exo- (#M0212L; NEB).
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bioRxiv - Genomics 2022Quote: ... a 5’ adapter was ligated onto the RNA transcript using T4 RNA Ligase I (NEB M0204L), and a third bead binding was performed ...
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bioRxiv - Genomics 2022Quote: ... a second bead binding was performed followed by a 5’ de-capping using RppH (NEB M0356S). The 5’ end of the transcript was phosphorylated using PNK (NEB M0201L ...
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bioRxiv - Microbiology 2022Quote: ... 5 μl of the cDNA was mixed with LongAmp Taq 2X Master Mix (New England Biolabs) and 100 pmol Sol-PrimerB (5’-GTTTCCCACTGGAGGATA-3’ ...
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bioRxiv - Molecular Biology 2022Quote: ... and 5 µg of HMW DNA were de-phosphorylated using Shrimp Alkaline Phosphatase (rSAP; NEB, M0371) for 30 min at 37°C followed by 5 min at 65°C ...
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bioRxiv - Molecular Biology 2023Quote: ... These were eluted and precipitated before ligation to 5’ adapters (1× T4 RNA ligase buffer (NEB), 0.5 µM p10IllLigup adapter ...
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bioRxiv - Plant Biology 2022Quote: ... We then ligated single-stranded rP5_RND adapters to 5’-ends with T4 RNA ligase 1 (NEB). Ligated RNAs were enriched and captured by oligo(dT ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA (5 µg in 20 µL ddH2O) was incubated with 2µL 100U Nuclease P1 (NEB) and 2 µL 10x Nuclease P1 buffer (NEB ...
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bioRxiv - Biophysics 2024Quote: ... R4: 5’-TAGGTACAGTCCTCAGGTGTCTT[AmC6dT]T) were coupled with SNAP-substrate NHS ester (BG-GLA-NHS, NEB). The DNA strands at 1 mM concentration were incubated with 10 mM of BG-GLA-NHS and reactions were cleaned up using a Bio-Spin P-30 column (Bio-rad).
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bioRxiv - Genetics 2024Quote: ... All probes were 5’ end-labeled with [alpha-32P]-ATP and T4 polynucleotide kinase (NEB Inc.). The gels were exposed to a Phosphor Imager ...
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bioRxiv - Microbiology 2024Quote: ... or INRKKR cRNA/mRNA (5’-CATGATTGTGTCAACCTGCTCTG) were radioactively labelled using T4 polynucleotide kinase (New England Biolabs) and ɣ-32P-ATP (PerkinElmer) ...
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bioRxiv - Immunology 2024Quote: ... 100 ng of gDNA was directly digested with 5 U of EcoRI-HF (New England Biolabs) in the ddPCR Supermix for Probes (no dUTPs ...
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bioRxiv - Cell Biology 2024Quote: ... Background assembly was measured by transformation of NEB 5-alpha competent cells (New England Biolabs C2987I) before cleanup of the assembly reactions using AmpureXP magnetic beads (Beckman Coulter A63880) ...
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bioRxiv - Microbiology 2024Quote: ... 5 units (2.5 μL) of RNase free DNAse I (NEW ENGLAND BioLabs, 2000 u. mL-1) and 100 μL of nuclease free water was added for every 10 μg of RNA ...
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bioRxiv - Biophysics 2023Quote: ... Topoisomerase I treated DNA was purified using Monarch PCR & DNA Cleanup Kit (5 μg) (NEB, T1030S) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 5 μl 10 mM MnCl2 buffer and 0 or 2 μl lambda phosphatase (NEB #P0753S, USA) in 38 μl supernatant ...
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bioRxiv - Molecular Biology 2023Quote: ... 6 µl aliquots were 5’ adapter ligated (25 µl of 1× T4 RNA ligase buffer (NEB), 1 mM ATP ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 μl 10 pM/μl and previously adenylated using the 5′ DNA adenylation kit (NEB, E2610S), and 1 μl T4 RNA ligase 2 truncated KQ (NEB M0373L) ...
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bioRxiv - Molecular Biology 2023Quote: ... CX plasmid (Bukhari et al., 2016) and 5′UTR containing amplicon was digested with NheI (NEB) followed by ligation with T4DNA ligase (NEB) ...
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bioRxiv - Cell Biology 2023Quote: ... and 62.5 μL of Lambda Protein Phosphatase (New England Biolabs, 5 U μg-1 of protein) were added with gentle mixing after each addition ...
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bioRxiv - Evolutionary Biology 2023Quote: ... supplemented with 10 units of Exonuclease we and 5 units of Lambda Exonuclease (New England BioLabs), was added to each sample ...
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bioRxiv - Molecular Biology 2023Quote: ... Library was PCR amplified for 5 cycles using the NEBNext High-Fidelity MasterMix (New England BioLabs) followed by qPCR amplification to determine the exact number of additional cycles required for optimal library amplification ...
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bioRxiv - Biochemistry 2023Quote: ... The HBB 5’ UTR containing mRNA was then capped using Vaccinia Capping enzyme (New England Biolabs) and 2′O-methylated using Vaccinia 2′O Methyltransferase (New England Biolabs) ...
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bioRxiv - Biophysics 2022Quote: ... The gene sequences were flanked by restriction enzyme sites for 5’ NdeI and 3’ EcoRI (NEB). Genes were cloned into pET21a using standard protocols from NEB ...
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bioRxiv - Genetics 2023Quote: ... All probes were 5’ end-labeled with [alpha-32P]-ATP and T4 polynucleotide kinase (NEB Inc.). The gels were exposed to a PhosphorImager ...
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bioRxiv - Molecular Biology 2023Quote: ... we treated the RNase I RPFs with 5 U of T4 PNK (NEB, 10 U/µL) after RNA size selection [19] ...
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bioRxiv - Cell Biology 2023Quote: ... cells were labeled with 5 μM cell-impermeable SNAP-Surface Alexa Fluor 488 (New England Biolabs) in complete media (DMEM + 10% FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Oligos were 5’-end radiolabeled at a final concentration of 4 μM with T4 PNK (NEB) and γ-32P-ATP after incubation for one hour at 37°C followed by enzyme inactivation at 72°C for 10 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... 5’ and 3’ homology arms and the insert were cloned into the pUC19 vector (NEB, #N3041S) by using Gibson Assembly Master Mix (NEB ...
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bioRxiv - Microbiology 2024Quote: ... was conducted after phosphorylating the 5’ end of the pooled fragments with T4 PNK (NEB, #M0201S). Reverse transcription and PCR amplification of the library were followed by PAGE-assisted size selection were carried out as previously outlined67 ...
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bioRxiv - Molecular Biology 2024Quote: ... The R1R DNA adapter was adenylated by using a 5’ DNA Adenylation kit (New England Biolabs) and then ligated to the 3’ end of the cDNA by using Thermostable 5’ App DNA/RNA Ligase (New England Biolabs ...
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bioRxiv - Bioengineering 2024Quote: ... 5 μl of clean DNA was transformed into 50 μl of DH5α commercial competent cells (NEB). The whole transformation was spread onto a 10 cm ampicillin agar plate ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by dA tailing with 0.25 U/µL Klenow (3′→5′ exo-) (New England Biolabs, M0212) in the presence of 0.5 mM dATP (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: 10 µg of DNase-treated total RNA was digested with 5 U of RNase H (NEB) and 2 picomoles of a chimeric oligonucleotide targeting the MSRs for 20 minutes at 37 ℃ ...
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bioRxiv - Molecular Biology 2024Quote: ... Amplification of target sequences under 5 kb were produced using Q5 high fidelity polymerase (NEB # M0491S) under the recommended three-step amplification conditions ...
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bioRxiv - Molecular Biology 2024Quote: ... Cloning strains used were NEB Stable (lentiviral) and NEB 5-alpha (other plasmids) (New England Biolabs). For base editor cloning ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was 5’-labelled with 20 U of T4 Polynucleotide Kinase (3’ phosphatase minus) (NEB # M0236S) and 50 pmol of (gamma-32P)ATP (Perkin Elmer ...