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Citations for New England Biolabs :
1251 - 1300 of 2067 citations for 5 Sulfosalicylaldehyde sodium salt since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... Free adaptor was then removed by addition of 1 μL 10 U/μL 5’-Deadenylase (NEB, M0331S). 1 μL 10 U/μL RecJ Exonuclease (Lucigen/Epicentre ...
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bioRxiv - Cell Biology 2021Quote: ... pStr-KDEL_APV-Dendra2/mCherry/EGFP-ER-5 was constructed by the Gibson assembly (New England BioLabs E2611) of pStr-KDEL_SBP-EGFP-Ecadherin linearized by AscI and XbaI ...
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bioRxiv - Microbiology 2020Quote: ... This plasmid was then digested with BamHI and NotI and the resulting insert (5’esaAflank_SpecPromoter_kanR_3’esaAflank) was gel extracted (Monarch DNA Gel Extraction Kit, NEB). 100 ng of purified insert was transformed into S ...
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bioRxiv - Immunology 2021Quote: ... The assembled plasmid pCO2 was transformed into NEB® 5-alpha competent Escherichia coli (High Efficiency) (NEB) according to manufacturer’s instructions (see Supplementary Figure 1 for plasmid maps) ...
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bioRxiv - Synthetic Biology 2021Quote: ... oPool oligos were phosphorylated at their 5’ ends using T4 Polynucleotide Kinase (New England Biolabs, Ipswich, MA). DNA adapters were then annealed to the phosphorylated oPool oligos by incubating extension ...
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bioRxiv - Biophysics 2021Quote: ... 5′-end capping and 3′-end biotinylation were performed using the Vaccinia Capping System (New England Biolabs) and the Pierce RNA 3′ End Biotinylation Kit (Thermo Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... DamID adaptors were removed by digesting overnight at 37° C with 5 units of Sau3AI (R0169S; NEB). The sequencing libraries were prepared according to the Illumina TruSeq nano DNA library protocol ...
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bioRxiv - Genetics 2022Quote: ... The 5’ adaptor (rArCrArCrUrCrUrUrUrCrCrCrUrArCrArCrGrArCrGrCrUrCrUrUrCrCrGrArUrCrU, IDT) was then ligated to the product using T4 RNA ligase 1 (NEB) at 15 °C for 4 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA fragments were directly 3’-end dephosphorylated using 5 U of Antarctic Phosphatase (New England Biolabs, UK) for 30 minutes at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... 0.2 μM reverse primer and 5 μL Luna® Universal Probe qPCR Master Mix (New England Biolabs)] amplified using the Prism7900HT sequence detection system (Applied Biosystems ...
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bioRxiv - Genetics 2022Quote: ... The 37nt long 5’ adaptor was ligated to the small RNAs using T4 RNA ligase (M0204S, NEB) overnight at 16°C ...
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bioRxiv - Genomics 2022Quote: ... was used in a 50 μl-reaction containing 5 μl of NEBuffer r3.1 (10X, NEB, cat# B7203), 26 μl of adapter-ligated DNA and NF water to 50 μl ...
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bioRxiv - Immunology 2022Quote: ... 5 mM DTT) prior to incubating with 0.5 μCi/μl [γ32P]ATP and T4 PNK (NEB, M0201L) to radiolabel crosslinked RNA ...
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bioRxiv - Molecular Biology 2022Quote: miRNAs were synthesized by IDT and radio-labeled at the 5’ end with T4 polynucleotide kinase (NEB) and (γ-32P ...
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bioRxiv - Microbiology 2022Quote: ... followed by adding a 5’ cap using the Vaccina Capping System (cat#M2080S, New England Biolabs Inc.) and a poly-A tail using E.coli Poly(A ...
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bioRxiv - Microbiology 2022Quote: ... 5 ng of α-HA ChIP or input DNA were end-repaired with T4 DNA polymerase (NEB), Klenow DNA polymerase (NEB) ...
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bioRxiv - Neuroscience 2022Quote: ... 45 nM pegRNA3-3AGTGA (Appendix Table S1) and 5 µl NEBuilder HiFi DNA Assembly Master Mix (NEB) and incubated at 37°C for 1 h.
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bioRxiv - Molecular Biology 2022Quote: Approximately 5 μg genomic DNA were digested with 1.5 μl restriction enzymes + 2.5 μl CutSmart buffer (NEB) for 5 hrs at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... After CIP inactivation (2 min at 80°C) piRNAs were 5΄end radiolabeled by T4 PNK (NEB) with [γ-32P] ATP (10mCi/mL ...
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bioRxiv - Bioengineering 2024Quote: ... 5 μL of PCR product from the inner primer amplification was cleaned using 5μL Exonuclease I (NEB) and 2μL rSAP (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was 5’-end-labeled with 32P using T4 polynucleotide kinase (New England Biolabs catalogue number M0201L). Samples were separated using 4–12% BisTris-PAGE and transferred to a nitrocellulose membrane (Protran) ...
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bioRxiv - Biochemistry 2024Quote: ... which was dephosphorylated at the 5’-end with 3 µl of Quick-CIP (5000 U/µl, NEB) in a total volume of 20 µl according to manufactureŕs instructions ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids were amplified using NEB 5-alpha F′Iq Competent Escherichia coli (High Efficiency) (NEB, Cat# C2992H), and isolated with PureYield Plasmid Miniprep System (Promega ...
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bioRxiv - Genomics 2023Quote: ... Reverse transcription reactions (RT +) were then performed with 5 µg of RNA and random hexamers (NEB, #S1330S), using the Superscript III Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... 5 μg of DNA was digested with HinfI and RsaI restriction enzymes (New England BioLabs, 50U each) at 37 °C overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... 5’-phosphorylated and dA-tailed with NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645). Samples were ligated to adaptor oligos for multiplex sequencing (NEB ...
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bioRxiv - Microbiology 2023Quote: ... pETduet-1::5′-flank_SpecPromoter_kanR_3′-flank and were subsequently digested from the plasmid using BamHI and NotI (NEB). The resultant insert (5′-flank_SpecPromoter_kanR_3′-flank ...
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bioRxiv - Evolutionary Biology 2023Quote: ... containing 12·5 μL of Q5 Hot Start High-Fidelity 2X Master Mix (New England BioLabs, M0494S), 1·1 μL of 10 μM SARS-Cov2-Midnight-1200 primer (either Pool 1 or Pool 2)(17 ...
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bioRxiv - Genetics 2023Quote: ... and amplified for 5 cycles using NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs; M0541L). After evaluation by real-time PCR ...
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bioRxiv - Microbiology 2023Quote: ... CVO689-CVO586 (integrant 5’ end) and CVO321-CVO183 (integrant 3’ end) using Q5 Polymerase (New England Biolabs). PCRs were set up according to manufacturers’ instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Beads were treated with JET (12.5 nM d-o-p) and/or ScaI-HF (NEB, 5 U) for 15 minutes at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Nuclei were centrifuged (500xg, 5 min, 4°C) and washed once with 1X NEBuffer 2.1 (NEB, #B7202). For nucleosome depletion ...
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bioRxiv - Biochemistry 2023Quote: ... VCE or FCE::T7RNAP fusion and 5 U/μL vaccinia cap 2′-O-methyltransferase (New England Biolabs). Reactions were carried out at indicated temperatures for 1 h ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Nuclei were pelleted at 500 ξ g at 4°C for 5 minutes and resuspended in 0.5 mL of 1.2x NEBuffer r2.1 (New England Biolabs) containing 3% sodium dodecyl sulfate (SDS ...
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bioRxiv - Neuroscience 2024Quote: ... 50 µg of protein lysate was treated with 5 µL NeuA (a2-3,6,8,9 Neuraminidase A, P0722L, NEB) or 5 µL of O-Glycosidase (P0733L ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The assembled plasmid was transformed into NEB 5-alpha (high efficiency) competent cells (New England Biolabs; C2987). After verifying the sequence ...
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bioRxiv - Bioengineering 2024Quote: ... Initial studies were done in an alternate NEB® 5-alpha strain (New England Biolabs, Catalog # C2987H) where trpA and trpB were replaced with a chloramphenicol resistance cassette ...
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bioRxiv - Physiology 2024Quote: ... Glycan digestion was achieved with the following enzyme concentrations: 5 μL of α2-3,6,8,9 neuraminidase A (20 U/μL, New England Biolab, NEB) with 1x GlycoBuffer 1 (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... then combined with 5 μM dNTPs and 2 μL of 10× rCutSmart buffer (NEB, Ipswich, MA, USA) in a total reaction volume of 17 μL ...
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bioRxiv - Molecular Biology 2024Quote: ... fragments were dephosphorylated by adding 5 μL antarctic phosphatase buffer and 2 μL enzyme (NEB cat#M0289S) and incubating for 1 h at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... Following KLD (kinase, ligase, and DpnI) treatment and transformation into NEB 5-alpha F’Iq cells (New England Biolabs), cells were grown as near-lawns on LB-chloramphenicol plates and a miniprep was performed from a resuspension of ~5000 colonies for each library.
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bioRxiv - Molecular Biology 2019Quote: ... 5 μL of Standard Taq Reaction Buffer (Final concentration 2.5×, 3.75 mM MgCl2, New England BioLabs, MA, USA), 1 μL of dNTPs (final concentration 0.5 mM ...
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bioRxiv - Genomics 2021Quote: ... the purified products are treated with Klenow fragment (3’ → 5’ exo-) (Cat. No. M0212L; NEB; use 1 uL) and Taq DNA polymerase (Cat ...
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bioRxiv - Biochemistry 2020Quote: ... the top strand (MB733) was 5′ labelled with ϒ[32P]- ATP and T4 polynucleotide kinase (T4 PNK; NEB) for 1 h at 37°C and cleaned up with a G-50 micro column as above ...
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bioRxiv - Developmental Biology 2021Quote: ... the synthesized sgB was injected at 300ng/µL after 5-minute incubation at RT with Cas9 protein (NEB) at 2µM final in 20mM Hepes-NaOH pH 7.5 ...
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bioRxiv - Developmental Biology 2021Quote: ... 5 μL (approximately 250 ng) of the extracted DNA was first digested using the restriction enzyme DpnII (NEB), diluted to 100 μL and ligated with T4 ligase (NEB) ...
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bioRxiv - Developmental Biology 2020Quote: ... 37.5 μl of 0.4 mM biotin-14-dATP and 10 μl of 5 U/ μl Klenow (NEB, M0210L) were added and mixed by pipetting ...
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bioRxiv - Molecular Biology 2020Quote: ... The RNA was then 5’ end labeled in 24μL PNK wash buffer with 16μL of 1x PNK buffer (NEB) containing 150μCi of gamma P32-ATP ...
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bioRxiv - Neuroscience 2021Quote: NFIB 3’ UTR and 5’ UTR HP forming regions were in vitro transcribed using T7 transcriptase (NEB, E2040) and purified with Trizol extraction (described in RT-qPCR paragraph) ...
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bioRxiv - Microbiology 2020Quote: ... RNA was denatured again and pyrophosphates were removed from the 5’-end by 10 U RppH (M0356S, NEB) in 1× NEB buffer 2 (10 mM Tris-HCl pH 7.9 ...