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Citations for New England Biolabs :
1001 - 1050 of 2029 citations for Recombinant E. coli LT IIc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... coli DNA ligase (New England Biolabs). After the second strand synthesis the cDNA of all single cells of a plate were pooled ...
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bioRxiv - Immunology 2024Quote: ... coli T7 express (New England Biolabs) cells ...
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bioRxiv - Genomics 2024Quote: ... coli Lemo21 (DE3) (New England Biolabs), which was grown at 30°C in lysogeny broth with 30 mg L-1 chloramphenicol and 50 mg L-1 kanamycin until an optical density (600 nm ...
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bioRxiv - Microbiology 2024Quote: ... coli DH5α(λpir) cloning strain (NEB) was propagated at 37°C in LB (1% tryptone ...
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bioRxiv - Biophysics 2023Quote: Escherichia coli Topoisomerase I (NEB, M0301) was used to relax a supercoiled circular DNA plasmid ...
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bioRxiv - Molecular Biology 2023Quote: ... coli using ultracompetent DH5α from NEB by heat shock as per manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... coli host cells (New England Biolabs) following an identical expression protocol ...
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bioRxiv - Biochemistry 2021Quote: ... 25 U Hi-T7 RNA polymerase (NEB #M0658), 250 nM RNaseAlert™ QC System v2 (ThermoFisher ...
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bioRxiv - Biochemistry 2022Quote: ... with recombinant proteins (200-500 ng) and yeast purified CK2 or recombinant human CK2 (NEB, P6010S). Proteins were resolved by SDS-PAGE and the gels were exposed to autoradiography or stained with Coomassie blue.
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bioRxiv - Bioengineering 2022Quote: Recombinant Cas9 (New England Biolabs, USA) was used to generate a standard curve (20µM ...
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bioRxiv - Physiology 2022Quote: ... Recombinant CK2 was purchased from NEB. Recombinant TGFBR1 was purchased from Proqinase ...
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bioRxiv - Microbiology 2024Quote: LT area was amplified from 12-week gDNA with PCR using Q5 High-Fidelity DNA Polymerase (NEB) and cloned to pUC19 vector to BamHI/HindIII site ...
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bioRxiv - Molecular Biology 2020Quote: ... MBP-tagged complexes were further purified on amylose resin (NEB). The core complex without MBP was instead further purified on Anti-Flag M2 affinity resin (Sigma) ...
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bioRxiv - Microbiology 2020Quote: ... MBP-tagged RBD was affinity purified by Amylose Resin (NEB) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... was cut with Hind III and Bam HI (NEB) for 1h at 37 °C ...
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bioRxiv - Biochemistry 2022Quote: ... and His-MBP-ZFC3H1 onto an Amylose resin (NEB). After extensive washing ...
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bioRxiv - Immunology 2021Quote: ... were assembled using Hi-Fi DNA Assembly Mix (NEB). For the SFFV-Cas9 vector ...
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bioRxiv - Biochemistry 2021Quote: ... Nb35–His (10µg/ml) and apyrase (25mU/ml, NEB); the suspension was incubated for 1h at room temperature ...
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bioRxiv - Synthetic Biology 2020Quote: ... or Hi-Fi assembly from New England BioLabs (NEB) and other basic molecular cloning approaches ...
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bioRxiv - Genomics 2021Quote: ... was then inserted by Hi-Fi assembly (NEB, E2621) using 0.025 pmols of vector and 0.05 pmols of the GFP amplicon ...
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bioRxiv - Cell Biology 2021Quote: ... using NEB Hi-Fi assembly mix (New England Biolabs). A mixture of the two guide plasmids (each at 100ng/µl ...
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bioRxiv - Developmental Biology 2023Quote: ... or Q5 Hi-Fidelity DNA Polymerase (New England Biolabs) was used ...
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bioRxiv - Biochemistry 2024Quote: ... We expressed the His-SUGCT in Lemo cells (NEB), inoculating 4L of TB with 4 mL overnight culture ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli cells (New England Biolabs cat. #C3019H) according to the manufacture’s recommendation ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli K12 DH5α cells (New England Biolabs) were used ...
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bioRxiv - Microbiology 2020Quote: ... coli BL21 (DE3) cells (New England Biolabs) and transformants were selected on LB agar supplemented with kanamycin (30 μg/mL) ...
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bioRxiv - Microbiology 2020Quote: ... coli poly(A) polymerase (New England Biolabs) following a recently published protocol44 ...
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bioRxiv - Microbiology 2021Quote: ... coli DNA ligase buffer (New England Biolabs), 1µL of E ...
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bioRxiv - Molecular Biology 2021Quote: ... coli strain BL21 (DE3) (New England Biolabs). From each of the two subcloned selection outputs ...
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bioRxiv - Biophysics 2022Quote: ... coli BL21 DE3 cells (New England Biolabs). One-liter cultures of Terrific Broth (TB ...
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bioRxiv - Molecular Biology 2021Quote: ... coli poly(A) polymerase (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... coli strain DH10B (New England Biolabs, C3019I) was used for transformations ...
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bioRxiv - Immunology 2021Quote: ... coli strain BL21(DE3) (New England BioLabs) using Overnite Express™ TB medium (Novagen ...
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bioRxiv - Synthetic Biology 2019Quote: ... coli cells (New England Biolabs, Ipswich, USA) were transformed as described in the following paragraph.
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bioRxiv - Microbiology 2019Quote: ... coli competent cells DH5α and ER1821 (NEB) used for respectively cloning and methylation of plasmid DNA were grown in LB medium (10 g/l tryptone ...
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bioRxiv - Bioengineering 2020Quote: ... coli strain BL21(DE3) (New England Biolabs). Plasmids were a gift from Kalina Hristova (Addgene #108856 ...
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bioRxiv - Plant Biology 2019Quote: ... coli BL21(DE3) strain (New England Biolabs) harboring the pET-28 expression vector (Novagen ...
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bioRxiv - Biochemistry 2019Quote: ... coli cells (New England Biolabs, Ipswich, MA) in LB or M9 minimal media supplemented with vitamins (Centrum) ...
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bioRxiv - Genomics 2019Quote: ... coli poly(A) polymerase (Cat. M0276S, NEB). The poly(A)-tailed Mb3Cas12a mRNAs were purified using the RNA Clean & Concentrator™-5 (Cat ...
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bioRxiv - Biophysics 2019Quote: ... coli strain BL21(DE3) (New England Biolabs,75-77 Knowl Piece ...
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bioRxiv - Biochemistry 2021Quote: ... coli BL21 (DE3) cells (New England Biolabs) in M9 enriched medium supplemented with 15NH4Cl (1g/L ...
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bioRxiv - Genetics 2021Quote: ... Escherichia coli (E.coli) DNA polymerase I (NEB), RNase H (NEB ...
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bioRxiv - Genomics 2020Quote: ... coli Poly(A) Polymerase (New England Biolabs) according to manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... coli DNA ligase (NEB, Cat. #M0205 L), 5 μl of E ...
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bioRxiv - Immunology 2021Quote: ... coli DNA Polymerase (NEB, Cat. #M0209 L), 1 μl of 10mM dNTP (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... coli JM109 strain (New England Biolabs (NEB)) was used ...
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bioRxiv - Bioengineering 2021Quote: ... coli JM109 strain (New England Biolabs (NEB)) was used ...
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bioRxiv - Bioengineering 2020Quote: ... coli (High Efficiency) cells (New England Biolabs) and plated onto LB plates containing 50 μg mL−1 kanamycin ...
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bioRxiv - Bioengineering 2020Quote: ... coli (High Efficiency) cells (New England Biolabs) were used for plasmid construction ...
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Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural productsbioRxiv - Biochemistry 2020Quote: ... coli DH 10-beta (New England Biolabs) was transformed with Gibson assembly product ...