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Citations for New England Biolabs :
1151 - 1200 of 1722 citations for 10 PROPOXY DECANOIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: Purified gDNA samples were diluted 10-fold and in parallel into Cutsmart-buffered BamHI-HF (New England Biolabs) restriction digestions in the presence of 0.1% Pluronic F-68 (50μL final volume ...
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bioRxiv - Genomics 2020Quote: ... 0.2 μL 10 mM dNTPs (Kapa Biosystems, Woburn, MA, 0.1 μL Q5 Polymerase (New England Biolabs, Ipswich, MA), 0.5 μL forward primer (10 μM) ...
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bioRxiv - Biophysics 2021Quote: ... The medium was then exchanged for 150 μL 10 nM SNAP-Dy549 (SNAP-Surface 549, New England Biolabs) in complete medium and incubated for a further 5 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Then 50 μl of DNase I mixture (25 μl of 10× DNase I buffer, 18 μl of NEB DNase I ...
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bioRxiv - Cell Biology 2019Quote: ... cells were collected and incubated in fresh SM containing 10 µM SNAP-Cell Block (New England Biolabs S9106), for 30 min at 25°C ...
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bioRxiv - Biochemistry 2019Quote: ... the fluidic cell was incubated with 10 mg/ml bovine serum albumin (BSA, New England Biolabs, Ipswich, USA) to prevent non-specific surface binding ...
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bioRxiv - Cell Biology 2019Quote: ... 5-10 µg of total protein (9 µl of lysate) were treated with Endo Hf (New England Biolabs) for 1 h at 37°C ...
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bioRxiv - Cancer Biology 2019Quote: ... UltraScript 2.0 (PB30.31-10, lot #PB130614-01-5, PCR Biosystems, Wayne PA) and WarmStart RTx (M0380L, lot #0061705, NEB) by each manufacturer’s protocol with minor modifications ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1.5 μL of 10 mM dNTPs mix,1.5 U of NEB Taq DNA polymerase (New England Biolabs, USA) and 10 pmol of each primer and 10-200 ng of DNA template ...
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bioRxiv - Evolutionary Biology 2020Quote: ... approximately 500 ng of total RNA was used for a 10 ul cDNA synthesis with ProtoScript II (NEB). 15 uM of the appropriate strand-specific primer (IDT ...
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bioRxiv - Synthetic Biology 2019Quote: ... PCR products were treated with 10 units (0.5 μL) of DpnI restriction endonuclease (New England Biolabs Ltd., #R0176), incubated at 37°C for 30 minutes ...
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bioRxiv - Synthetic Biology 2021Quote: ... The pooled duplex DNA (20 μL) was phosphorylated using T4 polynucleotide kinase (10 units; M0201S, New England Biolabs) in 10X T4 DNA ligase buffer (2 μL ...
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bioRxiv - Developmental Biology 2020Quote: ... Nuclei from pools of at least 10 distal or proximal limbs were digested with DpnII (New England Biolabs) and ligated with T4 DNA ligase HC (Promega ...
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bioRxiv - Molecular Biology 2021Quote: ... A 10% fraction of the chromatin lysate was digested using 200 U of Taq I (New England Biolabs) at 60°C for 7 h ...
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bioRxiv - Developmental Biology 2021Quote: ... 10 ng RNA were used for analysis with the LUNA one-step RT-qPCR kit (LUNA E3005L Biolabs). The relative expression levels of the mRNA of interest were determined by real-time PCR using Quantifast SYBR Green Mix (Qiagen ...
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bioRxiv - Molecular Biology 2021Quote: ... for 12 h at 4 °C with 10 μg of Factor Xa protease (New England Biolabs, Hitchin, UK) per 1 g of mitochondria ...
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bioRxiv - Microbiology 2020Quote: ... at final volume 20 μL with 10 μL of Luna Universal qPCR Master Mix (New England Biolabs, Germany). The concentration of the primers varied from 0.2-0.5 μM (for further details regarding genes ...
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bioRxiv - Molecular Biology 2020Quote: ... and crosslinks were reversed with 10% SDS and 0.6 mg/ml Proteinase K (New England Biolabs, Ipswich, MA) overnight with shaking at 65 °C ...
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bioRxiv - Genetics 2019Quote: ... and 2.0 units of the CspCI enzyme ((N)10-11CAA(N)5GTGG(N)12-13) (New England Biolabs). The library preparation was carried out according to the protocol proposed by Osorio-Guarín et al ...
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bioRxiv - Neuroscience 2021Quote: ... Phosphatase treated samples were enzymatically treated for 3 n at 37°C shaking (1.25 μl 10 μM MnCl2, 1.25 μl 20X NEB buffer ...
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bioRxiv - Genomics 2022Quote: ... 13.1 μL Murine RNAse inhibitor, 5.25 μL Tartrazine (10 mg/mL, Carl Roth) and 13.2 μL T4 DNA ligase (New England Biolabs). 4 μL of the master mix were added to the Ligation Barcoding Plate #1 or #2 (see above ...
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bioRxiv - Immunology 2022Quote: ... The nuclease-digested RNA was then dephosphorylated by adding 10 units/µg of Quick CIP enzyme (NEB, M0525) and incubating at 37 °C for 90 min (Kellner et al. ...
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bioRxiv - Microbiology 2022Quote: ... 1-20 μg of the Env protein was mixed with 1 μl of Glycoprotein Denaturing Buffer (NEB, 10×) and H2O (if necessary ...
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bioRxiv - Molecular Biology 2023Quote: ... and decapped with 10 U of T4 PNK +/− 40 nmol of ATP and 5 U of RppH (NEB). After each step ...
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bioRxiv - Neuroscience 2022Quote: ... 1 µl 10x BSA (diluted 1:10 with Milli-Q water from a 100X stock, New England Biolabs), and 1 µl of each enzyme used as described in Fig ...
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bioRxiv - Synthetic Biology 2024Quote: ... Golden Gate Assembly reactions were performed in a volume of 10 µL with 0.5 µL of either Esp3I (10,000 U/mL, NEB) or BsaI (10,000 U/mL Thermo Scientific) ...
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bioRxiv - Developmental Biology 2024Quote: ... and 1x 10 µl Phusion® High Fidelity PCR master Mix with HF buffer (Biolabs new England M0531S). The thermocycler program was 94 °C for 30 sec ...
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bioRxiv - Microbiology 2023Quote: ... Each peptide was tested for its ability to be cleaved by recombinant furin (10 U/mL; NEB; P8077) in a buffer of 100 mM HEPES ...
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bioRxiv - Microbiology 2024Quote: ... 5 μl of 10 mM MnCl2 and 1 μl (400 units) of λPP (New England Biolabs, Ipswich, MA). Untreated lysates received 1 μl of H2O in place of λPP ...
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bioRxiv - Microbiology 2024Quote: ... 1 mM DTT and 100 μM ATP) and incubated with 10 μM SNAP-Cell TMR-Star dye (NEB) for 1 hour at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... cells were harvested 3 days after electroporation and genomic DNA was extracted with 100 μL lysis buffer (10 mM Tris-HCl, pH 7.5, 0.05% SDS, 25 μg/mL proteinase K (NEB)) at 37 °C with incubation for 1 hour ...
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bioRxiv - Biophysics 2024Quote: ... A 4°C AKTA Pure FPLC system (Cytiva) was prepared with a 10 mL amylose column (NEB #E8021S) and 2 mL/min flow rate ...
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bioRxiv - Cell Biology 2024Quote: ... gibco) supplemented with 10% fetal calf serum (eurobio scientific) and 1% ZellShield cell culture contamination preventive (Minerva-Biolabs). Cells were passaged at 80-90% confluency ...
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bioRxiv - Genomics 2023Quote: ... Purified DNA (∼10 ng) was used to make libraries using the NEBNext Ultra II kit (New England Biolabs) and sequenced on the NextSeq 2000 (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... followed by 3’ A-addition and adapter ligation using a custom reagent formulation (New England BioLabs, E6000B-10). Libraries were pooled in equal molar amounts and were sequenced using an Illumina HiSeqX platform (Ilumina ...
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bioRxiv - Biophysics 2023Quote: ... the CBP-TEV sites was removed from plasmid pRS306 orc1-gal1-10-orc2 through Gibson assembly (NEB #E2611L) using primers TL-441 ...
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bioRxiv - Genomics 2023Quote: ... The recombination products were then electroporated into electrocompetent cells (NEB 10-beta, New England BioLabs, cat. No. C3020K) and plated onto Carbenicillin plates ...
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bioRxiv - Developmental Biology 2023Quote: ... Transcription reaction volume was reduced to 10 µl and 0.5 units of thermostable inorganic pyrophosphatase (New England Biolabs) was included in the reaction ...
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bioRxiv - Cell Biology 2023Quote: ... BsGrI-HF or AluI individually (10 U/μg DNA in CutSmart® buffer, all from New England BioLabs) overnight at 37°C ...
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bioRxiv - Genetics 2023Quote: ... (1.33x Gibson-Assembly Master-Mix [for 25 reactions]: 100 μL 5x Isothermal Reaction-Mix, 215.32 μL H2O, 0.2 μL T5 Exonuclease [10 U/μL NEB #M0363] ...
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bioRxiv - Biochemistry 2023Quote: ... first with 20 pmol membrane impermeable SNAP dye (10 μM SNAP-Surface® 488, New England BioLabs Inc.) followed by 20 pmol membrane permeable SNAP dye (10 μM SNAP-Cell® 647-SiR ...
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bioRxiv - Biochemistry 2023Quote: ... RNA capping enzyme assays were performed in 10 μL of 1x RNA capping buffer (New England Biolabs, Inc.) containing 0.5 μM substrate RNA ...
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bioRxiv - Developmental Biology 2023Quote: ... RNA was isolated from 10 hpf embryos using a Monarch total RNA miniprep kit (New England BioLabs Inc.) RNA was stored at −80°C until sample analysis and sequencing by the Molecular Evolution Core at Georgia Tech as previously reported(Johnson et al ...
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bioRxiv - Systems Biology 2023Quote: ... The concentrated ligated products were then transformed into NEB 10-beta Electrocompetent E.coli (New England Biolabs, Ipswitch, MA). Three transformations were performed to increase the yield ...
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bioRxiv - Microbiology 2023Quote: ... 10µM of each primer and 10 μl of OneTaq 2X Master Mix with Standard Buffer (New England Biolabs) was used for double-strand DNA synthesis ...
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bioRxiv - Molecular Biology 2023Quote: ... To a 10 μL aliquot of the supernatant were then added 2 μL of loading dye (NEB, #B7021S) and 1 μL of 2% sodium N-dodecanoylsarcosinate ...
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bioRxiv - Immunology 2024Quote: ... In brief, isolated Fc domains were denatured (10 minutes, 95C) prior to enzymatic glycan release with PNGaseF (NEB) per manufacturer’s instructions (18 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... 7.5 µl m7G(5′)ppp(5′)G RNA Cap Structure Analog (10 μmol) (New England BioLabs, Ipswich, MA), 2 µg linearized DNA ...
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bioRxiv - Bioengineering 2024Quote: ... along with a well containing 10 µl Quick-Load® Purple 1 kb DNA Ladder (New England Biolabs catalog no ...
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bioRxiv - Genetics 2024Quote: ... 0.5 µl of each primer (10 µM) and 12.5 µl Luna Universal Probe qPCR Master Mix (NEB, M3004) were mixed in a final volume of 25 µl ...