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Citations for New England Biolabs :
951 - 1000 of 1722 citations for 10 PROPOXY DECANOIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... 10-20 μL of bound DNA was used for library amplification using PCR (NEB, E7370S). Routinely 8-10 PCR cycles were used to generate enough amount of library DNA for sequencing ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 μl 10 mM dNTPs and 1 μl Bst 2 WarmStart DNA polymerase (NEB M0538S) was added and sample incubated 30 min at 65°C before precipitation with 12.5 μl 10 M NH4OAc ...
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bioRxiv - Microbiology 2022Quote: ... Then the tRNA were digested with 10 U Nuclease P1 (New England Biolabs, MA, USA) at 37°C overnight followed by dephosphorylation using 10 U bacteria alkaline phosphatase (Thermofisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... and Phusion DNA polymerase after 20 cycles of amplification (1.2×10−5) (NEB, product literature). Because the error rate from the RNA polymerase was much higher than the RT and PCR steps ...
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bioRxiv - Cell Biology 2022Quote: ... The reaction was initiated by addition of 10 U (pmol/min) of recombinant CK1 (NEB), CDK1-cyclin B1 (NEB) ...
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bioRxiv - Immunology 2022Quote: ... and incubated with a ligation reaction mixture (10 unit/μL T4 DNA ligase (NEB, M0202M), 1x T4 DNA ligase buffer ...
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bioRxiv - Plant Biology 2022Quote: ... RT-PCR was performed with 10 ng of total RNA using OneTaq (New England Biolabs) PCR reaction system with the primers listed in Table S3 ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 μM Rad51 and 10 μM (nucleotide) φX174 circular single-stranded DNA (ccsDNA) (N3023L, NEB) were mixed in reaction buffer (25 mM Tris-HCl [pH 7.5] ...
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bioRxiv - Evolutionary Biology 2022Quote: ... transformations that involved the CRE library were performed using electroporation (NEB 10-beta electrocompetent E.coli), in all other cloning steps chemically competent E ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was further purified on a 5-10 mL amylose column (New England Biolabs) in 50 mM Tris-HCl (pH 7.8) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The 10-µl reactions contained: 5 µl Luna Universal qPCR Master Mix (New England Biolabs), 0.25 µl forward primer (10 µM) ...
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bioRxiv - Molecular Biology 2024Quote: ... The cosmid-I95 plasmid was amplified in a NEB 10-beta (New England Biolabs, C3019H), and the DNA was purified using a QIAfilter Plasmid Midi Kit (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... resuspended in 500 μL 10 mM Tris-HCl buffer and digested with DpnII (NEB, R0543) overnight at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... The eluent was subsequently mixed with 10 U of Bst 2.0 WarmStart DNA polymerase (NEB) and 1× Q5 polymerase reaction buffer (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... 10 μg of plasmids were then digested overnight with the restriction enzyme CspCI (NEB, #R0645S) in a total volume of 50 μL ...
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bioRxiv - Synthetic Biology 2023Quote: ... according to manufacturer’s protocols and transforming chemically competent NEB 10-beta cells (NEB CN# C3019), where colony PCR and Sanger sequencing were again used to determine if the clone was correct.
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bioRxiv - Molecular Biology 2022Quote: ... single cells were lysed in 100 nL lysis mix (10 nL 1X Cutsmart buffer (NEB), 10 nL Proteinase K (Ambion) ...
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bioRxiv - Microbiology 2022Quote: ... for 10 min and treated with PNGase F or Endo Hf enzymes (New England BioLabs) for 1.5 h at 37°C in accordance with the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... the ligation reaction was performed by introducing 10 units T4 DNA ligase enzyme (NEB, USA) and incubating at 20 °C for 1 hour to seal the 5’ and 3’ ends of the ssPCRP ...
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bioRxiv - Molecular Biology 2023Quote: ... samples were incubated with 1.5 µl EndoH and 2 µl 10× GlycoBuffer 3 (all NEB) buffer at 37°C 1h ...
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bioRxiv - Zoology 2023Quote: ... The amplified fragment and UAS plasmid (pSL*attB:YFP:Gyp:UAS14i:Gyp:attB) (10) were ligated following EcoRI and XhoI (NEB) digestion and purification ...
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bioRxiv - Biochemistry 2023Quote: ... the first aliquot was further treated with 10 U of PNGaseF (New England BioLabs, P0705S) for 8 hr at 37 °C.
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bioRxiv - Plant Biology 2023Quote: ... 1 μg of purified DNA was treated with 10 units Exonuclease V (New England Biolabs) in a 50 μl reaction at 37°C for 30 min to remove ssDNA ...
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bioRxiv - Bioengineering 2022Quote: ... coli NEB 10-β and BL21 (DE3) strain (both from New England BioLabs, Ipswich, USA) with pRI952 plasmid used for all fermentations ...
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bioRxiv - Developmental Biology 2023Quote: ... Reactions (10 μl) were performed Luna® Universal qPCR Master Mix (New England Biolabs, M3003X). mRNA abundance for each gene was determined relative to GAPDH mRNA using the 2−ΔΔCt method ...
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bioRxiv - Genomics 2023Quote: ... 100uM Oligos were annealed using 10× T4 DNA ligase reaction buffer (New England Biolabs, B0202S), and T4 Polynucleotide Kinase (New England Biolabs ...
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bioRxiv - Biochemistry 2023Quote: ... while the other was digested at 25 °C with 10 units SwaI (New England Biolabs). Enzymes were inactivated at 65 °C for 20 min ...
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bioRxiv - Bioengineering 2023Quote: ... Rectangle DNs were folded with 10 nM of M13mp18 ssDNA scaffold (Bayou Biolabs, P-107) and annealed in 20x excess staples ...
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bioRxiv - Biophysics 2023Quote: ... The mixture was then combined with 15 uL 10 mM dNTPs (New England Biolabs; #N0447S) and 1 uL SUPERase-In (ThermoFisher AM2696 ...
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bioRxiv - Biochemistry 2023Quote: ... 1 μg of one plasmid was digested overnight with 10 units PacI (New England Biolabs) in 20 μL at 37 °C ...
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bioRxiv - Biochemistry 2024Quote: ... Purified products were again transformed into either NEB 10-beta (New England Biolabs, Ipswich, MA) or SIG10 (Sigma-Aldrich ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We transformed the purified HiFi reaction into 10-beta electrocompetent cells (NEB, Cat. No. C3020K) with a BioRad MicroPulser Electroporator (Cat ...
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PD-1 checkpoint blockade disrupts CD4 T cell regulated adaptive B cell tolerance to foreign antigensbioRxiv - Immunology 2021Quote: ... 10 ng of genomic DNA was amplified using LongAmp Taq 2X Master Mix (New England BioLabs) and the provided barcoded 16S primers ...
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bioRxiv - Cell Biology 2019Quote: ... We incubated cells in complete medium containing 10 μM of SNAP-Cell Block (New England Biolabs) to block all available pre-existing SNAP-tag (Quench) ...
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bioRxiv - Genetics 2021Quote: ... we digested 10 μg of each vector with AvrII (#ER1561; Thermo Fischer) and XcmI (#R0533; NEB) and performed a gel-purification ...
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bioRxiv - Genomics 2020Quote: ... 1 μL of 10 mM dATP and 15 units of Klenow 3’ → 5’ exo- (NEB M0212L) was added to blunted ends and incubated at 37°C for 30 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... The 5’-end of dephosphorylated mRNAs were then labeled using 10 units of T4 PNK (NEB) and 0.5 µl [γ-32P] ATP (6000 Ci/mmol ...
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bioRxiv - Molecular Biology 2020Quote: 10 μg of genomic DNA was digested overnight with 20 units of AluI and MboI (NEB) and then precipitated with isopropanol ...
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bioRxiv - Genomics 2022Quote: ... 10 μl 2X Quick T4 ligase buffer and 2 μl Quick T4 DNA ligase (NEB, M2200L) were added to the reaction and incubate at 37 °C for overnight ...
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bioRxiv - Genomics 2020Quote: ... PCR amplification (8-9 cycles) was performed with 10 μL Fusion HF Buffer (New England Biolabs), 3.125 μL 10uM TruSeq Primer 1 ...
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bioRxiv - Genomics 2020Quote: The polyadenylation product was mixed with 0.5 μL 10 mM dNTPs (New England Biolabs, Cat. # N0447L), 1 μL reverse transcription primers (25 ng/μL ...
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bioRxiv - Genetics 2019Quote: ... The reannealed amplicon was then digested with 10 units of T7 Endonuclease I (New England Biolabs) at 37 °C for 90min ...
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bioRxiv - Neuroscience 2019Quote: ... and then mounted using 10 μl ProLong Gold Antifade Reagent containing DAPI (9071S, New England Biolabs). The slides were imaged when the mounting medium was fully cured >12 h.
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bioRxiv - Molecular Biology 2019Quote: ... 10 mM CaCl2 and 1x PIC) containing 2U/μl of micrococcal nuclease (New England BioLabs, M0247S) and incubating on ice for 20 min and then 37°C for 10 min ...
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bioRxiv - Cancer Biology 2020Quote: ... 20 μl reaction mixture composed by 10 μl of Phusion High-Fidelity Master Mix 2X (NEB), 2 μl of Betaine 5M (Sigma-Aldrich) ...
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bioRxiv - Physiology 2021Quote: ... RNA 3’ end dephosphorylation reaction consisted of T4 PNK (20 U/10 μL sample, NEB M0201S), SuperaseIn in 1X T4 PNK buffer without ATP for 60 min at 37 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... 10 µl of PCR product was then mixed with 1.5 µl 10X NEBuffer 2 (B7002S, NEB) and 1.5 µl of Nuclease-free water ...
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bioRxiv - Genomics 2021Quote: ... DNA samples were labeled with 10 μL of a cocktail of repair enzymes (PreCR mix, NEB), fragmented ...
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bioRxiv - Genomics 2020Quote: ... using 10 units each of MluCI and NlaIII restriction enzymes (New England Biolabs, Beverly MA, USA), NEB CutSmart buffer ...
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bioRxiv - Bioengineering 2020Quote: ... The post-transcription reaction mixture was incubated with 10 units of DNase I (New England Biolabs) at 37°C for 1 hour to remove the gblock template then purified using a RNA spin column (Zymo Research) ...