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Citations for New England Biolabs :
1101 - 1150 of 1722 citations for 10 PROPOXY DECANOIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... Free adaptor was then removed by addition of 1 μL 10 U/μL 5’-Deadenylase (NEB, M0331S). 1 μL 10 U/μL RecJ Exonuclease (Lucigen/Epicentre ...
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bioRxiv - Microbiology 2021Quote: ... and 10 μl of 2x Quick T4 ligase buffer and 1 μl Quick T4 DNA ligase (NEB) were added and incubated at room temperature overnight ...
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bioRxiv - Genetics 2019Quote: ... eluted with 50 ul of 1x NEB Buffer 3 and treated with 10 units of CIP (NEB) for 1 hour at 37°C ...
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bioRxiv - Genomics 2020Quote: ... 10 million crosslinked cells were lysed and the chromatin digested using 375 U of MboI (NEB, R0147M) for 4 hours at 37°C ...
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bioRxiv - Genetics 2020Quote: ... IP and input samples were incubated with 10 μL of 800 U/mL Proteinase K (NEB P8107S) at 37° for 2 hours ...
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bioRxiv - Bioengineering 2022Quote: ... All ligation reactions were performed in a 10 µL mix with T4 DNA Ligase (New England Biolabs) at 16°C overnight according to the manufactureŕs instructions.
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bioRxiv - Biochemistry 2022Quote: ... 50 pmol of in vitro transcribed or synthetic RNA was incubated with 10 units T4 PNK (NEB) and 2 μl γ-32P-ATP in 1X PNK buffer and incubated for 1 h at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... 10 min) and resuspended in 50 µl of 50 mM Tris-HCl containing 1 µg trypsin (NEB). After overnight incubation at 37 °C ...
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bioRxiv - Microbiology 2024Quote: ... followed by DNaseI treatment of the RNA for 10 min at 37°C (New England Biolabs, M0303), and removal of the DNaseI using the RNA clean up kit (New England Biolabs ...
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bioRxiv - Microbiology 2024Quote: ... followed by DNaseI treatment of the RNA for 10 min at 37°C (New England Biolabs, M0303), and removal of the DNaseI using the RNA clean up kit (New England Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ... 1.5 ul 10 mM dGTP, 1.5 ul 10 mM dTTP, 37.5 ul 0.4 mM biotin-14-dATP, 40 ul NEB buffer2 ...
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bioRxiv - Genetics 2024Quote: ... 10 μg of the purified RNA was then polyadenylated by 4U poly(A) polymerase (New England Biolabs) at 37°C for 1 hr and purified again by illustra microspin G-50 column or Direct-zol RNA Miniprep kits (Zymo Research ...
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bioRxiv - Synthetic Biology 2023Quote: ... 1 μg RCA product was restricted by employing 10 U nicking endonuclease Nb.BbvCI (New England Biolabs, R0631L) in a 50 μL reaction volume with 1x rCutsmart buffer for 1 h at 37 °C ...
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bioRxiv - Genomics 2023Quote: ... ligation master mix was added (10x NEB T4 DNA ligase buffer with 10 mM ATP B0202-NEB); 10% Triton X-100 ...
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bioRxiv - Biochemistry 2023Quote: ... The reactions were quenched by adding 10 μL of 3X SDS loading dye (New England Biolabs, Inc.). The quenched reactions were analyzed by SDS-PAGE on a 10-20% Tris-Glycine SDS polyacrylamide mini-gel (ThermoFisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 ng DNA was used to prepare libraries using the NEBNext Ultra II DNA kit (NEB E7645S) as described with modifications ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA 3′-ends were dephosphorylated in a 10 µL reaction with 2 µL T4 PNK (NEB #M0201S) 1 µL of FastAP Thermosensitive Alkaline Phosphatase (ThermoFisher #EF0651) ...
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bioRxiv - Molecular Biology 2023Quote: RNA 3′-ends were dephosphorylated in a 10 µL reaction with 2 µL T4 PNK (NEB; #M0201S) 1 µL of FastAP Thermosensitive Alkaline Phosphatase (ThermoFisher #EF0651) ...
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bioRxiv - Synthetic Biology 2023Quote: ... XL-10 Gold competent Escherichia coli (E. coli) cells and SOC Outgrowth Medium were purchased from NEB. ATP ...
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bioRxiv - Microbiology 2023Quote: ... 10 µl of purified PCR product was used in a 20 µl HiFi assembly reaction (NEB, E2621S). HiFi assembly was performed at 50°C for 1 hour.
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bioRxiv - Genomics 2023Quote: ... and digested with 10 units of uracil–DNA glycosylase (New England Biolabs, Ipswich, MA; cat. no. M0280L). Amplification of the libraries was performed for 13 PCR cycles using the Phusion High-Fidelity PCR Master Mix (New England Biolabs ...
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bioRxiv - Biochemistry 2023Quote: ... the resin was mixed with 10 μM SNAP-Cell® TMR-Star (New England Biolabs Inc., #S9105S) at room temperature for 10 minutes to label the SNAPf-tag ...
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bioRxiv - Biophysics 2024Quote: ... 10 µl of each sample was mixed with 4 µL native loading dye (1% Orange G (NEB) dissolved in 50% glycerol and 50% EMSA buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 µl genomic DNA (input 90 ng) from naïve hESCs were digested with LpnPI (New England Biolabs) generating 32 bp fragments around the fully methylated recognition site containing a CpG ...
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bioRxiv - Bioengineering 2023Quote: ... and 1.25 μl of 10 μM each primer using Phusion high-fidelity DNA polymerase (New England BioLabs). After PCR ...
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bioRxiv - Immunology 2023Quote: ... 2.5 μL of 10 μM P7 primer and 0.5 μL of Phusion Polymerase (NEB, cat. no: M0530L). The qPCR was run using the following conditions ...
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bioRxiv - Systems Biology 2023Quote: ... 22 μL of a mixture containing 10 mM of each of the four deoxynucleotide triphosphates (dNTPs) (NEB), 5.5 μL of TSO primer (100 μM) ...
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bioRxiv - Genomics 2023Quote: ... at 10 pmol each and then amplified in 9 cycles using Phusion DNA polymerase (New England Biolabs) from primers that added flanking overlaps to tiles allowing Infusion cloning in a screening vector ...
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bioRxiv - Bioengineering 2023Quote: ... and 1.25 μl of 10 μM each primer using Phusion high-fidelity DNA polymerase (New England BioLabs). After PCR ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting plasmid products were transformed into NEB 10-beta electrocompetent cells (New England Biolabs, Ipswich, MA) and purified using a ZymoPURE Midiprep Kit (Zymo Research ...
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bioRxiv - Biochemistry 2024Quote: ... Nicking mutagenesis plasmid products were transformed into NEB 10-beta electrocompetent cells (New England Biolabs, Ipswich, MA) and purified using a ZymoPURE Midiprep Kit (Zymo Research ...
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bioRxiv - Cell Biology 2024Quote: ... existing CENP-A-SNAP proteins were labelled for 30 min using 10 µM SNAP-Cell Block (NEB) and cells were exposed to media containing 5 µM S-trityl-L-cysteine (STLC - Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... then combined with 5 μM dNTPs and 2 μL of 10× rCutSmart buffer (NEB, Ipswich, MA, USA) in a total reaction volume of 17 μL ...
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bioRxiv - Microbiology 2024Quote: ... 20 pmoles of dephosphorylated RNA was combined with 2 μl 10 x T4 Polynucleotide Kinase buffer (NEB), 3 μl ATP(y-32P ...
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Higher meiotic chromosome condensation: a potential function of kinetochore through polo-like kinasebioRxiv - Cell Biology 2024Quote: ... TCA extract in Laemmli buffer was diluted 10-fold in 1X PMP buffer (NEB, Protein Metallo Phosphatases) supplemented with 1 mM Mncl2 ...
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bioRxiv - Cancer Biology 2024Quote: Plasmids at concentration 10-25 ng/µl together with I-SceI meganuclease and NEB buffer (NewEngland BioLabs), were co-injected into the blastomere at one-cell stage embryos ...
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bioRxiv - Microbiology 2024Quote: ... 50 pmol RNA was dephosphorylated with 10 units of calf intestine alkaline phosphatase (#M0290, New England Biolabs) in a 50 μl reaction at 37°C for 1 h ...
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bioRxiv - Molecular Biology 2021Quote: ... the reaction incubated with Exonuclease I (2U, add 1μl of a 10-fold dilution of NEB Exonuclease I) for 15 minutes at 37°C ...
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bioRxiv - Evolutionary Biology 2021Quote: 3’ dephosphorylation was performed by incubating fragments with 10 U/uL T4 Polynucleotide Kinase (New England Biolabs M0201S) in the supplied buffer (NEB B0201S ...
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bioRxiv - Developmental Biology 2020Quote: ... 37.5 μl of 0.4 mM biotin-14-dATP and 10 μl of 5 U/ μl Klenow (NEB, M0210L) were added and mixed by pipetting ...
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bioRxiv - Genomics 2020Quote: ... 1 mM ATP and 10 mM DTT and with 1 μl (2 U/μl) of RNase H (NEB), 1 μl (3 U/μl ...
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bioRxiv - Molecular Biology 2021Quote: ... The cell lysis was cleared by centrifugation at 16,100g for 10 min and 0.65 ml supernatant was mixed with 50μl Protein G beads (NEB, #37478S) pre-conjugated to U1-70K antibody (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... RNA was denatured again and pyrophosphates were removed from the 5’-end by 10 U RppH (M0356S, NEB) in 1× NEB buffer 2 (10 mM Tris-HCl pH 7.9 ...
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bioRxiv - Molecular Biology 2019Quote: ... About 2-3 μg of source DNA plasmid (<10 kb) was added with NEBuffer 3.1 (NEB, cat# B7203S), DEPC ddH2O in a volume of 30 μl and incubated at 37 °C for >2 hour (h) ...
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bioRxiv - Genomics 2019Quote: ... 50 µL of NEB Buffer 3.1 and 20 µL of 10 U/µL DpnII restriction enzyme (NEB, R0147) were then added ...
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bioRxiv - Systems Biology 2019Quote: ... then flow in 2mL of wash buffer (10% ethylene carbonate in TBS with 2mM Vanadyl Ribonucleoside Complex (NEB)) ...
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bioRxiv - Microbiology 2020Quote: ... followed by 10 cycles of annealing at 54°C for 30 s) using Taq polymerase (New England Biolabs) according to the manufacturer’s instructions and the primers 5-CTTGACATCTCTGAGGCAAC-3 and 5-ATGCAGGGGCAAAGTCATTAG-3 ...
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bioRxiv - Microbiology 2021Quote: Each peptide was tested for its ability to be cleaved by recombinant furin (10 U/mL; NEB; P8077) in a buffer of 100 mM HEPES ...
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bioRxiv - Synthetic Biology 2021Quote: ... Each reaction consisted of 10 μl of Luna Universal Probe qPCR Master Mix (New England Biolabs, Ipswich, MA), 5 μl of standard or sample ...
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bioRxiv - Microbiology 2021Quote: ... 100ng of vector was digested for 1 hour at 37°C with 10 U of BamHI-HF (NEB) to create a single double-stranded cut or 10 U Nb.BtsI (NEB ...