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Citations for New England Biolabs :
1101 - 1150 of 2329 citations for Human PRPF31 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... SUMO1 and SUMO3 were amplified from human cDNA and cloned in the pTXB1 vector (New England Biolabs) by restriction cloning according to the manufacturers protocol.
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bioRxiv - Cancer Biology 2023Quote: ... Human RAC1P29S cDNA was cloned into the vector using EcoRI and XhoI restriction enzymes (New England Biolabs) and ligated using T4 ligase (New England Biolabs) ...
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bioRxiv - Developmental Biology 2022Quote: Complementary DNA (cDNA) encoding for Human CD36 (RC221976) was cloned using Hifi DNA assembly kit (NEB E5520S) into the pJFRC-MUH vector (Addgene ...
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bioRxiv - Microbiology 2024Quote: ... and libraries were prepared using the NEBNext rRNA Depletion Kit for Human/Mouse/Rat (NEB Cat#E6310). Adapter-ligated cDNA was amplified with 11 cycles of PCR to generate libraries ∼300 bp in length ...
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bioRxiv - Biochemistry 2024Quote: The cloning of human wild type Mad2 and the mutant Mad2R133A by the USER® method (NEB) into the pRSFDuet-1 vector (71341-3 ...
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bioRxiv - Biophysics 2021Quote: ... The GRB2 mutagenesis plasmid (pGJJ057) was assembled by a ligation reaction (T4 Ligase, New England Biolabs) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... 400-800 ng of the plasmid was mixed with the BsaI-HFv2 restriction enzyme (NEB, R3733S) in a 10 μL reaction volume (1 μL BsaI-HFv2 enzyme ...
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bioRxiv - Microbiology 2021Quote: ... the plasmid digestion was supplemented with 1.5 units of recombinant shrimp alkaline phosphatase (rSAP; NEB #M0371S). Digested insert and vector were purified and ligated together at room temperature for 30 min using T4 DNA ligase (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting PCR product was ligated into the pMal-c2x protein expression plasmid (New England Biolabs) adjacent to the malE gene to generate the pMAL-c2x-malE-L375-his10 plasmid encoding maltose binding protein (MBP ...
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bioRxiv - Synthetic Biology 2022Quote: Isolated no recombinase OR gate plasmid (250 ng) was incubated with purified Cre recombinase (M0298S; NEB) at 37 °C for 1.5 hours followed by a 10-minute heat inactivation at 70 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR fragments used for plasmid construction were amplified by Q5 DNA polymerase (M0491, New England Biolabs) according to manufacturer’s instruction ...
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bioRxiv - Cell Biology 2022Quote: ... and cloned in frame into Ch plasmid with AsiSI and NotI restriction endonucleases (New England BioLabs). The mammalian expression constructs for RFP-PCNA ...
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bioRxiv - Synthetic Biology 2019Quote: ... Both the PCR product and plasmid were digested by EcoRI HF and KasI (New England BioLabs) and purified ...
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bioRxiv - Synthetic Biology 2020Quote: ... Three microliters of prepared plasmid were linearized for integration in a twenty microliter NotI-HF (NEB) reaction for one hour and then added to the transformation mixture without purification ...
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DNA writing at a single genomic site enables lineage tracing and analog recording in mammalian cellsbioRxiv - Synthetic Biology 2019Quote: ... the plasmid to integrate the hgRNA into 293T cells was digested with EcoRI-HF (NEB#R3101), then purified on a silica column (Epoch #3010) ...
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bioRxiv - Microbiology 2019Quote: ... this DNA insert was ligated into plasmid pSRKV004 cut with BamHI and EcoRV (New England BioLabs). The plasmid pSRKV004 is a derivative of the integrating plasmid pSET152 (Hopwood ...
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bioRxiv - Microbiology 2019Quote: ... pGRA1plus-HPT-3xHA plasmid [43] was first digested using EcoRV-HF and NcoI (New England Biolabs) for 4 hrs at 37 °C to remove the GRA1 promoter ...
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bioRxiv - Synthetic Biology 2019Quote: ... and subsequently cloned in to the pMini-T plasmid using the PCR Cloning Kit (NEB #E1202). Plasmids were extracted from E.coli JM109 transformed cells using the QIAprep Spin Miniprep Kit (Qiagen ...
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bioRxiv - Microbiology 2019Quote: ... Plasmids were generated by Gibson assembly of PCR fragments using the NEBuilder HiFi Master Mix (NEB) or in vivo assembly (31) ...
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bioRxiv - Genetics 2019Quote: ... the duplexes were ligated into the BbsI site of the cut pX260 plasmid (New England Biolabs) and transformed into DH5-Alpha E ...
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bioRxiv - Microbiology 2021Quote: ... The pGHJ-TgApiAT6-1 and pGHJ-TgApiAT1 [27] plasmids were linearized by digestion with NotI (NEB) for 2 hr ...
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bioRxiv - Developmental Biology 2021Quote: ... 6-8 µg of Cas9-mSA plasmid was linearized by Not1-HF restriction enzyme (NEB #R3189S). The 8.8kb linearized fragment was cut out from a 1.5% agarose gel and DNA was extracted using QIAquick Gel Extraction Kit (Qiagen #28115) ...
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bioRxiv - Bioengineering 2021Quote: ... The amplicons and the linearized plasmid were assembled using an NEBuilder HiFi DNA assembly kit (NEB). The assembled plasmid was named pLenti-NG-PE2-BSD.
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bioRxiv - Biochemistry 2020Quote: ... The 186 bp fragment was excised from the plasmid by restriction digest with EcoRV (NEB: R0195) and EcoRI (NEB ...
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bioRxiv - Biochemistry 2020Quote: ... the amplified DNA and the P4H-TM isoform 1 plasmid were both digested with BlpI (NEB) and XbaI (NEB ...
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bioRxiv - Synthetic Biology 2021Quote: ... Backbone plasmids were purified from gel after digestion using the Monarch DNA Gel Extraction Kit (NEB). Ligations were carried out in 96-well PCR plates by mixing 0.2 μL of digested plasmid (3-9 ng μL−1 ...
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bioRxiv - Genetics 2021Quote: All plasmids were constructed using Gibson-cloning with the NEBuilder HiFi DNA Assembly master mix (NEB). For the construction of the N-terminal part of the split NG-ABEmax-encoding vector (AAV-NT-ABEmax) ...
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bioRxiv - Microbiology 2021Quote: ... the plasmid-Safe DNase-treated DNA was amplified using phi29 DNA polymerase (New England Biolabs, USA) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... The pST76-A plasmid was cut with fast digest BsaI (New England Biolabs, Ipswitch, MA, USA) followed by phosphatase treatment (Fast AP ...
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bioRxiv - Biophysics 2021Quote: ... the agarose-gel purified assembled insert and plasmid backbone were digested with BamHI and XhoI (NEB) and the insert ligated with T4 ligase ...
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bioRxiv - Biophysics 2021Quote: Purified plasmids were digested at the 3’ end of the insert sequence with EcoRI-HF (NEB) to linearize the template with a 5’ overhang for in vitro transcription ...
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bioRxiv - Cell Biology 2022Quote: Transforming plasmids were linearized by digestion with either ScaI-HF® or AseI restriction enzymes (NEB), which cut within the ampicillin resistance gene ...
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bioRxiv - Biophysics 2022Quote: ... 2.5 kilo base pair DNA tethers were PCR-amplified from the pUC19 plasmid (New England Biolabs) with a 5’-biotinylated primer on one side and a 5’-phosphoprimer on the other side ...
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bioRxiv - Developmental Biology 2022Quote: ... into the linearized PacI-AvrII pUASt-attB plasmid using ligation T4 strategy (New England Biolabs, Inc.).
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bioRxiv - Microbiology 2021Quote: ... the entire pGE450 plasmid was amplified by inverse PCR using Q5 High Fidelity DNA polymerase (NEB) with oligonucleotide TGD4981 and TGD4982 ...
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bioRxiv - Microbiology 2021Quote: ... the entire pGE190 plasmid was amplified by inverse PCR using Q5 High Fidelity DNA polymerase (NEB) with oligonucleotides TGD4006 and TGD5162 ...
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bioRxiv - Microbiology 2021Quote: Complementation plasmid pMQ70_lasA was generated using the NEBuilder HiFi DNA assembly cloning kit (New England BioLabs). P ...
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bioRxiv - Neuroscience 2020Quote: cDNA of tau 151-254 was inserted into a pMCSG17 plasmid using HiFi assembly (NEB #E2621S). The resulting vector encoded a protein consisting of a hexahistidine tag followed tobacco etch virus (TEV ...
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bioRxiv - Plant Biology 2021Quote: ... Plasmids were isolated from positive colonies using Monarch Miniprep kit (New England Biolabs, Ipswich, MA, US) and then FastDigest XbaI or XhoI separately (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... The 15.6-Kb ANEp8_Cas9_LIC1 plasmid was initially digested with the NotI restriction enzyme (New England Biolabs) to liberate a 5.3-Kb fragment containing the AMA1 cassette (purified by gel extraction using an Invitrogen PureLink Quick Gel Extraction Kit ...
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bioRxiv - Microbiology 2021Quote: ... Plasmids constructed in this study were obtained by using NEBuilder HiFi DNA assembly (New England Biolabs) method ...
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bioRxiv - Genomics 2022Quote: ... and ligated with the aforementioned triple-digested plasmid pool using T4 DNA ligase (New England Biolabs) at 16°C overnight with a insert:vector molarity ratio of 20:1 ...
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bioRxiv - Cancer Biology 2022Quote: ... was sub-cloned via restriction digest of the plasmid with NsiI-HF (NEB, Cat. No. R3127) and SnaBI (NEB ...
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bioRxiv - Biochemistry 2022Quote: ... Plasmid pl21 (TriAltus) was truncated by digestion with restriction enzymes XbaI and XhoI (New England Biolabs). To complete the recombinant assembly reaction NEBuilder® HiFi DNA Assembly kit (New England Biolabs ...
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bioRxiv - Molecular Biology 2022Quote: ... or GFP plasmid respectively were digested with XhoI for 1hr (New England Biolabs, Ipswich, MA, USA) for linearization ...
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bioRxiv - Bioengineering 2019Quote: ... ligation products enclosing fragments of non-integrated transposon donor plasmid DNA were digested with DpnI (NEB) in 50 µl overnight ...
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bioRxiv - Synthetic Biology 2019Quote: ... and inserts and plasmid were ligated at a 1:1 molar ratio with Quick Ligase (NEB) for 20 min at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... a mini-CTX-lux (TcR) plasmid (38) was digested with EcoRI and BamHI (New England BioLabs). The promoter region of mexAB-oprM operon was amplified with EcoRI_PmexAB_Fw and BamHI_PmexAB_Rv primers ...
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bioRxiv - Zoology 2019Quote: ... The pCMV-BI empty plasmid was digested with DraIII-HF (Cat. Nr. R3510, New England Biolabs) and NcoI-HF (Cat ...
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bioRxiv - Synthetic Biology 2020Quote: ... Plasmids were assembled using NEBuilder® HiFi DNA Assembly Master Mix following manufacturer’s protocol (NEB, MA). All plasmid sequences were confirmed by DNA sequencing (Eton Bioscience ...