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Citations for New England Biolabs :
951 - 1000 of 2519 citations for Human PRPF31 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: All plasmids were cloned using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) and mutations were made using the Q5 Site-directed mutagenesis kit (New England Biolabs) ...
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bioRxiv - Microbiology 2021Quote: ... Digested plasmid was then de-phosphorylated with Shrimp Alkaline Phosphatase (rSAP) (New England Biolabs) for 30 min at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Most of the plasmids were assembled using the NEBuilder HiFi DNA assembly mastermix (NEB) following manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2021Quote: ... The inserts were cloned into pLRE::GFP plasmid linearized with BamHI (NEB, Catalog # R0136) and SalI (NEB ...
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bioRxiv - Cancer Biology 2021Quote: ... Single gRNAs were cloned into LentiCRISPR 2.1 plasmid [57] by BsmBI (New England BioLabs) digestion and Gibson Assembly (New England BioLabs) ...
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bioRxiv - Neuroscience 2020Quote: ... The ensuing plasmid was then mutated using the New England Biolabs (NEB, Ipswich, MA) mutagenesis Q5 kit in 29 different reactions ...
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bioRxiv - Biochemistry 2019Quote: ... Plasmids were amplified by transformation into chemically competent Escherichia coli DH5α (New England Biolabs), which was plated onto LB agar (10 g/L tryptone ...
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bioRxiv - Biophysics 2019Quote: ... we performed a double digest of TH1007 and the pSNAPf plasmid (New England Biolabs) with AgeI and BamHI ...
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bioRxiv - Molecular Biology 2020Quote: ... and inserted into the opened plasmid with NEBuilder reagents (New England Biolabs; Berverley, MA) to create plasmid pBbRad51NLHAglmS (pBRNLHg ...
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bioRxiv - Biochemistry 2019Quote: ... Nanobody and opto-nanobody plasmids were transformed into Shuffle T7 Express E.coli cells (NEB) and eGFP and mCherry plasmids were transformed into One Shot Top 10 cells (Invitrogen) ...
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bioRxiv - Developmental Biology 2020Quote: ... and cloned into an SEC backbone plasmid (NEBuilder HiFi DNA Assembly Master Mix, NEB). The modified Cas9/sgRNA plasmid ...
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bioRxiv - Molecular Biology 2020Quote: ... This U6-2xBsmBI-Tracr-HygroR plasmid was digested with the BsmBI restriction enzymes (NEB), after which the CRISPR inserts were ligated in using T4 DNA ligase (NEB) ...
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bioRxiv - Molecular Biology 2021Quote: ... The subcloned AGO plasmids were transformed into Escherichia coli BL21 (DE3) (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... Plasmids were assembled using Gibson assembly (NEBuilder HiFi DNA Assembly Cloning Kit, NEB E5520S). Fragments were either PCR amplified with a least 15 bp overlap at each end (Phusion Hot Start Flex NEB M0535L ...
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bioRxiv - Developmental Biology 2021Quote: ... and PCR was performed on plasmid DNA with Phusion High Fidelity PCR Kit (NEB). PCR products were converted to RNA using the T7 RNA Transcription Kit (NEB ...
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bioRxiv - Molecular Biology 2019Quote: All MKRN1 mutant plasmids were generated with the Q5 Site-Directed Mutagenesis Kit (NEB) according to the manufacturer’s recommendations ...
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bioRxiv - Genetics 2020Quote: ... The purified linear plasmid was then dephosphorylated using Alkaline Phosphatase Calf Intestinal (CIP) (NEB) to ensure the linear plasmid did not ligate with itself ...
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bioRxiv - Microbiology 2021Quote: ... and inserted to the intermediate plasmid using NEBuilder HiFi DNA Assembly Master Mix (NEB). The L-sNTurboID fragment was ligated back to the pCEZ-L backbone using the same restriction sites Pac1 and Hpa1 by NEB T4 ligase.
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bioRxiv - Cell Biology 2022Quote: ... Ten micrograms of plasmid template were linearized by restriction with MluI (New England Biolabs) and purified using the Nucleospin Gel and PCR Clean-Up kit (Macherey-Nagel) ...
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bioRxiv - Biophysics 2021Quote: ... A plasmid containing two BsaI sites was first treated with BsaI (New England Biolabs), then separated on a 1% agarose gel ...
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bioRxiv - Cell Biology 2021Quote: ... Assembly of the final donor plasmid was performed by Gibson Assembly (New England BioLabs). sgRNA targeting near the 3’-end stop codon of FOXO3 were designed using CHOPCHOP (http://chopchop.cbu.uib.no ...
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bioRxiv - Microbiology 2020Quote: ... The amplicon was cloned in the pXDC39 plasmid vector using XbaI (New England Biolabs) and PstI (New England Biolabs) ...
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bioRxiv - Microbiology 2020Quote: ... plantarum B21 plasmids using OneTaq® 2X Master Mix with Standard Buffer (NEB, USA) in a T100™ Thermal Cycler (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... Derivatives of this plasmid were made using a Q5 site-directed mutagenesis kit (NEB). Single copy deaD-lacZ fusions were made by recombining the plasmid-borne fusions with modified λ phage ...
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bioRxiv - Genetics 2020Quote: Repair template plasmids were assembled from overlapping fragments using the NEBuilder HiFi Kit (NEB) per manufacturer instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... The PCS2-Rab11S25N-mCherry plasmid was generated using Q5 Site-Directed Mutagenesis Kit (NEB) with PCS2-Rab11-mCherry plasmid and Rab11S25N-mCherry-Fwd (TGTGGGGAAGaatAACCTGCTGT ...
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bioRxiv - Cell Biology 2022Quote: ... pRS306-GFP-linker-CDC42 plasmid was linearized with PshAI (Cat#R0593S, New England Biolabs) and co-transformed with a PCR product containing the desired K-to-R changes into an uracil auxotrophic strain (PC538) ...
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bioRxiv - Immunology 2022Quote: HIV envelope plasmids were transformed and amplified in NEB-stable competent cells (NEB, C3040H). Pseudoviruses were produced by co-transfection of plasmids encoding various HIV-1 Envs together with NL4-3 ΔEnv or Q23-ΔEnv (1:3 ratio ...
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bioRxiv - Synthetic Biology 2022Quote: ... and plasmids were constructed by using Q5 High-Fidelity DNA polymerase (New England Biolabs) to produce amplicons and ligating amplicons to PCR amplified vectors using Golden Gate DNA assembly.59 The broad range RSF1010 backbone was used for all plasmids and was given generously by Shyam Bhakta.60 All plasmid sequences were verified with Sanger sequencing.
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bioRxiv - Neuroscience 2022Quote: ... The plasmids were digested with appropriate restriction enzymes (New England BioLabs, Ipswich, MA, USA) under optimal conditions ...
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bioRxiv - Molecular Biology 2023Quote: ... and inserted into the pGLuc Mini-TK 2 Gaussia luciferase enhancer reporter plasmid (NEB) via KpnI/SacI restriction sites ...
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bioRxiv - Microbiology 2022Quote: ... using a pCAGGS plasmid digested with EcoRV-HF and HindIII-HF (New England Biolabs) as backbone ...
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bioRxiv - Neuroscience 2023Quote: ... Plasmids were constructed using standard restriction enzyme cloning or Gibson assembly (New England BioLabs). All plasmids used in this work are described in Supplementary file 2.
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bioRxiv - Microbiology 2022Quote: ... coli host strain using the Monarch® Plasmid Miniprep Kit (NEB, Ipswich, MA, USA). The extracted plasmid was linearized with restriction enzyme BamHI (Promega ...
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bioRxiv - Biochemistry 2024Quote: ... The plasmid was verified by digestion with the NotI restriction enzyme (New England Biolabs), according to manual.
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bioRxiv - Bioengineering 2024Quote: ... All A-packaged effector plasmids were generated using an isothermal assembly reaction (NEB M5520AA) (13).
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bioRxiv - Genomics 2024Quote: ... dCas9-VP16(10x) plasmid was linearized with restriction enzyme AflII (New England Biolabs R0520S). Successful integration of the dCas9-VP16(10x ...
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bioRxiv - Biochemistry 2024Quote: Plasmids required for generating mutant strains were prepared using HiFi DNA assembly (NEB, USA) and then transformed into S ...
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bioRxiv - Microbiology 2023Quote: ... Xho1 digestion of the plasmid stock was followed by phosphatase treatment using rSAP (NEB) to dephosphorylate the cut ends of the plasmid ...
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bioRxiv - Molecular Biology 2024Quote: The pJMP1339 plasmid DNA was digested using the BsaI HF-v2 Restriction endonuclease (NEB) for four hours at 37 ºC ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasmids were amplified following heat-shock transformation of NEB-5alpha competent cells (#C2992H, BioLabs), and purified with the NucleoBondXtra Maxi Plus kit (#740416 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... pBBR1-E and pBBR-EV were extracted using Monarch Plasmid Miniprep kit (#T1010; NEB) and following supplier’s instructions ...
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bioRxiv - Genomics 2024Quote: ... 1 μg of the step one plasmid library was linearized with XhoI (NEB #R0146) at 37°C for 16 hours ...
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bioRxiv - Genetics 2024Quote: ... we subcloned the small plasmid with restriction enzymes AgeI and BssHII (New England Biolabs) into a plasmid with full length SCN5A and again Sanger sequence verified the plasmids ...
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bioRxiv - Immunology 2024Quote: Maxi-prepped plasmids containing the replicate barcoded libraries were digested using SalI-HF (NEB) to linearize the plasmid prior to long-read sequencing ...
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bioRxiv - Microbiology 2024Quote: ... the linear plasmid was blunted by digestion with Quick Blunting™ Kit (NEB, USA) and ligated to re-circularize the plasmid ...
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bioRxiv - Microbiology 2024Quote: ... The fragments were cloned into a pBSIIKS+ plasmid digested with SacI and BamHI (NEB) using Infusion (Takara ...
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bioRxiv - Molecular Biology 2024Quote: ... The digested plasmid and the hybridised oligonucleotides were ligated using the T4 ligase (NEB) and then transformed in Escherichia coli HST08 competent cells (Takara) ...
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bioRxiv - Biochemistry 2024Quote: ... Plasmid growth was performed by transformation into NEB5α competent cells (New England Biolabs, USA) following manufacturer’s instructions and subsequent selection on LB agar plates with ampicillin (100 µg/mL final ...
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bioRxiv - Cell Biology 2023Quote: ... the plasmids were phosphorylated by T4 Polynucleotide Kinase and ligated by T4 Ligase (NEB).