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Citations for New England Biolabs :
1051 - 1100 of 2519 citations for Human PRPF31 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... The phosphorylated part amplicon and PCR amplified plasmid were ligated with T4 ligase (NEB) for 2 h at room temperature in a 4:1 ratio according to the manufacturer guidelines ...
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bioRxiv - Cell Biology 2021Quote: ... Snap tagged human D1DR constructs were labelled with Snap-cell 647 SiR (New England Biolabs, S9102S) as described previously (94).
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bioRxiv - Biophysics 2022Quote: ... The human μOR and V2R were amplified with a SNAP tag at their N-terminal (NEB) and subcloned in the pcDNA4/TO plasmid (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... Clean RNA was first rRNA depleted using the NEBNext rRNA depletion kit (Human/Mouse/Rat) (NEB) before being prepared for sequencing using the NEBNext Ultra II Directional RNA Library Prep Kit (NEB ...
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bioRxiv - Plant Biology 2021Quote: Ni pull down experiments were performed using 3μl of His tagged OsSRT1 constructs (0.3μg/ul) and 1μg recombinant human Histone H3 (NEB) and mixed with 20 μl of Ni-NTA slurry (Qiagen) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Ribosomal RNA was removed using NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) (New England Biolabs) according to the manufacturer’s instructions with 6 µl total RNA used as input per sample ...
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bioRxiv - Biochemistry 2022Quote: ... with recombinant proteins (200-500 ng) and yeast purified CK2 or recombinant human CK2 (NEB, P6010S). Proteins were resolved by SDS-PAGE and the gels were exposed to autoradiography or stained with Coomassie blue.
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bioRxiv - Neuroscience 2022Quote: ... made from pCX with the promoter replaced by human Synapsin (hSyn) using golden gate assembly (NEB). For synaptic labelling pCAG-GPHN-FingR-EGFP-CCR5TC40 Addgene # 46296 ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA-seq libraries were prepared using the NEBNext rRNA Depletion Kit v2 (Human/ mouse) (NEB #E7405), NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760 ...
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bioRxiv - Biophysics 2021Quote: ... and cloned into the pET24 expression plasmids using a Gibson Assembly Mastermix (New England Biolabs). All constructs contain an N-terminal Met-Gly-Ser and a C-terminal His6 tag ...
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bioRxiv - Cell Biology 2020Quote: ... the pHRRA1-GFP (pKS85) (Table 4) plasmid was PCR-amplified with Phusion HF Polymerase (NEB), using primers designed using the QuikChange Primer Design tool (Agilent) ...
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bioRxiv - Immunology 2021Quote: ... The recombinant plasmids (LentiCRISPRv2.gRNA10, LentiCRISPRv2.gRNA11) were then transformed into competent Escherichia coli (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... the plasmid was transformed into BL21 (DE3) Escherichia coli bacteria (New England BioLabs, catalog # C2527H) and grown overnight in 30 μg/ml kanamycin Terrific Broth (TB ...
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bioRxiv - Genomics 2020Quote: ... Alternate allele plasmids were generated via site directed mutagenesis (Q5 SDM kit, New England Biolabs) using the following primers ...
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bioRxiv - Neuroscience 2021Quote: The plasmid trans-Tango MkII was generated using HiFi DNA Assembly (New England Biolabs #2621) and was incorporated into the attP40 locus using the ΦC31 system as described in the original trans-Tango paper4 ...
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bioRxiv - Molecular Biology 2020Quote: ... Mutations in the pRS315-Rpc160 plasmid were generated using the Gibson Assembly Cloning Kit (NEB) and clones were confirmed by sequencing the entire coding region ...
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bioRxiv - Microbiology 2022Quote: ... MRR1a complementation plasmids containing the correct sequences were linearized with Not1-HF (New England BioLabs), cleaned up with the Zymo DNA Clean & Concentrator kit (Zymo Research ...
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bioRxiv - Microbiology 2022Quote: ... auris MRR1a complementation plasmid that had been previously linearized with NotI-HF (New England BioLabs) and Cas9 ribonucleoprotein containing crRNA targeting the NAT1 gene ...
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bioRxiv - Genomics 2020Quote: ... Repair template plasmids were assembled using the NEBuilder Hifi DNA Assembly Kit (New England BioLabs) to incorporate homology arms into the loxP + Pmyo-2∷GFP∷unc-54 3’UTR + Prps-27∷neoR∷unc-54 3’UTR + loxP dual-marker selection cassette vector (provided by Dr ...
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bioRxiv - Biochemistry 2020Quote: ... Plasmids are based on pFB001(31) and constructed by User Cloning™(New England Biolabs) in a three-way ligation method ...
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bioRxiv - Cell Biology 2019Quote: Plasmids were constructed using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, Ipswich, MA). All plasmids used in this study are described in Supplementary Table 2.
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bioRxiv - Bioengineering 2019Quote: ... The 3.8 kb pRS413 vector backbone was PCR amplified from plasmid pRS413 (New England Biolabs) using primer pair RS413-Fw/RS413-Rev for all yeast assembly reactions ...
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bioRxiv - Genomics 2020Quote: ... We digested the Lenti-gRNA-Puro plasmid with BsmBI restriction enzyme (New England Biolabs, NEB) for 12 h at 55 °C ...
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bioRxiv - Genomics 2020Quote: ... We digested the Lenti-gRNA-Puro plasmid with BsmBI restriction enzyme (New England Biolabs, NEB) for 12 h at 55 °C ...
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bioRxiv - Cell Biology 2019Quote: ... and cloned into the pL4440 plasmid using the T4 DNA Ligase (New England Biolabs, M0202). The cloned plasmid was transformed to E ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli strain DH5α was used for plasmid construction while SHuffle T7 Express (New England Biolabs) 20 was used for cyclonal expression and library selections ...
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bioRxiv - Synthetic Biology 2019Quote: ... PCR products and digested plasmids were purified with the Monarch PCR & DNA Cleanup Kit (NEB). Plasmids were purified using the QIAprep Spin Miniprep Kit (QIAGEN).
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bioRxiv - Biophysics 2021Quote: A 2.5 kbp DNA fragment was PCR amplified from the pUC19 plasmid (New England Biolabs) with a double digoxigenin-labeled primer (Biomers GmbH ...
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bioRxiv - Neuroscience 2020Quote: ... The Ps2Ex3 pDR274 plasmid was linearized with HindIII-HF ® (NEB, Ipswich, Massachusetts, USA, R3104S), and then used as a template for synthesis of Ps2Ex3 sgRNA with the MAXIscript™ T7 Transcription Kit (Ambion ...
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bioRxiv - Molecular Biology 2021Quote: Plasmids were transformed and grown in high efficiency DH5α competent cells (New England Biolabs C2987) before extraction by Miniprep (QIAGEN #27104) ...
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bioRxiv - Molecular Biology 2021Quote: ... and cloned into either BbsI-digested or Esp3I-digested plasmid by T4 DNA ligase (NEB) at 16°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... 30ug of transposon-containing genomic plasmid was digested with NotI-HF (New England Biolabs, USA) for 3 hours to remove the transposon body ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was generated from the pHH21-NA flag plasmids with Q5 Hot-Start PCR (NEB). This cDNA product was then cloned into the mammalian expression vector pCAGGS for transient transfection experiments as previously described [51].
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bioRxiv - Microbiology 2021Quote: The pCDNA3.1-hACE2-FLAG plasmid and a Q5 Site-Directed Mutagenesis Kit (New England Biolabs) were used according to the manufacturer’s specifications to generate recombinant plasmids that expressed hACE2 N-glycosylation mutants ...
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bioRxiv - Microbiology 2020Quote: ... The digested plasmid was then incubated twice with Antarctic Phosphatase (New England Biolabs, Ipswich, MA) to prevent self-ligation of cut plasmid ...
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bioRxiv - Cancer Biology 2020Quote: ... Plasmids were digested with BsmBI and BamHI in Cut Smart Buffer (New England BioLabs, Inc.) at 37°C for 1 hour and then analyzed by 1% agarose gel ...
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bioRxiv - Biophysics 2021Quote: ... plasmid containing the ctSTIM1 insert was transformed to BL21 competent cells (New England Biolabs #C25271) for pET28a plasmids ...
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bioRxiv - Biophysics 2020Quote: ... 2.5 kbp and 1.3 kbp DNA tethers were generated by PCR from pUC19 plasmid (NEB) with a double digoxigenin- or biotin-labeled primer on one side and a phosphoprimer on the other side ...
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bioRxiv - Neuroscience 2022Quote: ... The plasmids were used to amplify the UTRs with Phusion High-Fidelity DNA polymerase (NEB) using corresponding primers carrying SfiI and NheI restriction sites to allow the inserts between SV40 promoter and the reading frame of hRluc ...
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bioRxiv - Cell Biology 2022Quote: ... the same plasmid was linearised with ApaI and transcribed with SP6 RNA polymerase (both NEB). ‘Full length’ tert cDNA was amplified using primers FW ...
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bioRxiv - Biochemistry 2022Quote: ... The recombinant plasmid was generated by a NEBuilder® HiFi DNA Assembly (New England Biolabs) reaction ...
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bioRxiv - Genetics 2022Quote: ... We digested backbone plasmid BFA022425 with the restriction enzymes HpaI and BsmBI (New England Biolabs) to remove the backbone vector’s existing gRNA ...
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bioRxiv - Synthetic Biology 2022Quote: ... Plasmids were constructed by Gibson Assembly using NEBuilder® HiFi DNA Assembly Master Mix (NEB) or ligation using T4 DNA ligase (NEB) ...
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bioRxiv - Genomics 2022Quote: ... the PCUP1-Sup35N-Aß42 plasmid was linearised by PCR (Q5 high-fidelity DNA polymerase, NEB) with primers that remove the WT Aß42 sequence (primers MS_03-04 ...
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bioRxiv - Cancer Biology 2022Quote: ... Plasmids containing NHEJ or HR reporter cassettes were linearized by I-SceI restriction enzymes (NEB) and purified using GeneJET PCR purification kit (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... Any modifications to plasmid sequences were generated by Q5 site-directed mutagenesis (New England BioLabs) and confirmed by sequencing ...
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bioRxiv - Bioengineering 2020Quote: The pGRA-AP2IX1-V5 plasmid for AP2IX1 transient expression was created using Gibson assembly (NEB) from the pGRA-V567 ...
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bioRxiv - Microbiology 2020Quote: Virus-encoding plasmids were generated using Gibson Assembly (NEB Gibson Assembly kit, NEB, Ipswich, MA). Insert amplicons containing 25 bp overlaps to target regions were generated using Kapa HiFi Hotstart™ high-fidelity polymerase (Kapa Biosystems/Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: Virus-encoding plasmids were generated using Gibson Assembly (NEB Gibson Assembly kit, NEB, Ipswich, MA). Insert amplicons containing 25 bp overlaps to target regions were generated using Kapa HiFi Hotstart™ high-fidelity polymerase (Kapa Biosystems/Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2019Quote: ... 750 ng of transcribed plasmids were then incubated in the CutSmart Buffer (New England Biolabs) containing appropriate restriction enzymes and 2 U of RNase H ...