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Citations for New England Biolabs :
9951 - 10000 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... 100 ng of microbial gDNA was fragmented using Covaris S2 instrument to a mean size of 250 bp and used as input to the NEBNext Ultra II kit (E7645 New England Biolabs Ipswich, MA) and checked for quality using the Agilent Bio-analyzer 2100 and Qubit spectrofluotometer ...
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bioRxiv - Pathology 2021Quote: ... flanks fragments of bcptp1 gene were amplified and respectively cloned into the upstream and downstream regions of hph cassette using Gibson Assembly Master Mix kit (New England Biolabs, Massachusetts, USA). The overexpression plasmid OEBcPTP1- pH2G was generated that the full-length open reading frame of bcptp1 was cloned into the pH2G vector under the regulation of B ...
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bioRxiv - Plant Biology 2021Quote: ... We used 10 ng of IP or input DNA for ChIP-Seq library construction using NEBNext® Ultra DNA Library Prep Kit for Illumina® (New England Biolabs) according to manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2021Quote: ... the mutant gfp1a and gfp1b were created by inserting a gblcoks containing frame shift mutation sequences at the 5’ end of GFP coding sequence of pGFPGUSPlus (Plasmid #64401 in addgene) using the NEBuilder HiFi DNA Assembly Cloning Kit (New England BioLabs, Catalog #E5520S). Then ...
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bioRxiv - Plant Biology 2021Quote: ... RNA-seq libraries were then prepared from the mRNA using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Massachusetts, USA). Libraries were quantified by a fluorimetric method and their quality assessed on a Fragment Analyzer (Agilent Technologies) ...
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Cis-regulatory divergence underpins the evolution of C3-C4 intermediate photosynthesis in MoricandiabioRxiv - Plant Biology 2021Quote: ... The DNA fragment was inserted into pCambia1381 by homologous recombination using the Gibson Assembly Cloning kit (New England Biolabs, catalog number: E5510S). The predicted promoter region of the PHOT2 gene of M ...
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bioRxiv - Plant Biology 2021Quote: ... #ltp1.4ltp1.8-1 and #ltp1.4ltp1.8-2) were chosen for constructing next generation sequencing libraries following the manufacture’s protocol (NEB Next Ultra II DNA kit). Sequencing was carried out using 2 × 150 paired-end NextSeq500 (1-2 Mio reads for all samples together ...
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bioRxiv - Cell Biology 2021Quote: ... Amplification of cDNA was conducted using T7 forward primers and cDNA-based RNA was generated using HiScribeTM T7 Quick High-Yield RNA Synthesis kit (New England Biolabs, Ipswich, MA). T7 RNA (1 μg ...
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bioRxiv - Cell Biology 2021Quote: ... and amino-terminal mutants were generated in the hCTR1-p416TEF construct by site-directed mutagenesis using Q5® Site-Directed Mutagenesis Kit (NEB #E0554) protocol ...
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bioRxiv - Cell Biology 2021Quote: ... The Hi-C library for Illumina sequencing was prepped by NEBNext® Ultra™ II DNA library Prep Kit for Illumina (NEB) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... One μg of total RNA was used in NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, Frankfurt, Germany), followed by sequencing to obtain 14 to 29 mio ...
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bioRxiv - Microbiology 2020Quote: DNA fragments (150–250 bp) were selected for library construction and sequencing libraries prepared using the NEBNext ChIP-Seq Kit (NEB, catalog#: E6200). The libraries were sequenced using the HiSeq XTen (Illumina) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We prepared separate sequencing libraries for each individual using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Massachusetts, USA) following manufacturer instructions ...
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bioRxiv - Plant Biology 2020Quote: ... and blotted to a membrane followed by hybridization with radioactive probe [62] prepared using the random primers labelingNEBlot® kit (New England Biolabs, USA). The band detection was carried out using Typhoon® phosphor imager (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... we separated the PCR products from the parental genomic DNA by gel electrophoresis and purified the PCR fragments using the Monarch DNA Gel Extraction Kit (New England Biolabs, Evry, Fr). The purified fragments were then mixed and fused together using assembly PCR carried out with the Q5 high-fidelity DNA polymerase ...
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bioRxiv - Plant Biology 2020Quote: ... 10 ng of IP or input DNA was used for ChIP-Seq library construction using NEBNext® Ultra DNA Library Prep Kit for Illumina® (New England Biolabs) according to manufacturer’s recommendations ...
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bioRxiv - Synthetic Biology 2021Quote: ... The Hi-C library for Illumina sequencing was prepared using the NEBNext® Ultra™ II DNA library Prep Kit for Illumina (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Libraries for next-generation sequencing (NGS) were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, Ipwich, MA) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Genes of interest were cloned into the pLenti CMVTRE3G eGFP Neo (w821-1) plasmid using a Gibson Assembly Master Mix kit (Cat# E2611S, New England Biolabs, Ipswich, MA). pLenti CMV rtTA3 Blast (w756-1 ...
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bioRxiv - Microbiology 2020Quote: ... native barcode ligation using the EXP-NBD103 barcode kit (Oxford Nanopore Technologies, Oxford, UK) and Blunt/TA Ligase master mix (New England Biolabs, Ipswich, MA) was performed as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Fragmentation and library construction of the resulting PCR products were performed using an NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs, Ipswich, MA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... mRNA Magnetic Isolation Module (E7490S) and library preparation was completed using NEBNext® Ultra Directional RNA Library Prep Kit for Illumina® (E7420L, New England Biolabs), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... the purified cDNA was PCR amplified and barcoded using ONT’s PCR Barcoding Expansion 1-12 (EXP-PBC001) or PCR Barcoding Expansion 1-96 (EXP-PBC096) kits with LongAmp Taq 2x Mastermix (NEB, Ipswich, MA).
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bioRxiv - Microbiology 2021Quote: ... were cloned into the pCR-Blunt-II vector using the PCR Blunt Topo kit according to the manufacturer’s instructions and transformed into 10-beta CaCl2-chemically competent bacteria (originally NEB, generated in-house). Following amplifications of colonies and purification of plasmids using a QiAprep Mini kit (Qiagen) ...
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bioRxiv - Systems Biology 2021Quote: ... RNA-seq libraries were constructed with poly(A) mRNA enrichment and rRNA depletion processes using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs, Ipswich, MA). The indexed libraries were multiplex-sequenced in duplicate using two flow cells ...
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bioRxiv - Microbiology 2021Quote: ... where RNA metagenomic libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA). Libraries were pair-end sequenced (2×150 bp ...
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bioRxiv - Plant Biology 2021Quote: ... mRNA was isolated from the total RNA with an NEB Next poly(A) mRNA Magnetic Isolation Module Kit (New England Biolabs, MA, USA). RNA-seq libraries were synthesized using an NEB Next Ultra RNA Library Prep Kit for Illumina and an NEB Next Adaptor for Illumina Kit (New England Biolabs ...
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bioRxiv - Plant Biology 2020Quote: ... Libraries were constructed from 100 ng and 1 μg total RNA using the NEBNext UltraTM Directional RNA Library Prep Kit for Illumina for use with the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, Hitchin, UK). mRNA was isolated ...
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bioRxiv - Plant Biology 2020Quote: ... Site-directed mutagenesis was performed to change Arg106 to a histidine (His) using Q5® Site-Directed Mutagenesis Kit (New England Biolabs Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... ATG-free versions of the truncated OpIE2 promoter sequence (tIE2 promoter with no ATG triplet in its sequence) were created by Q5 Site-Directed Mutagenesis (SDM) Kit (New England Biolabs-NEB, USA). Primers used to generate mutations were designed using the NEBaseChanger tool (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries for sequencing were made from high quality RNA that were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, MA, USA) following manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2020Quote: ... Isolated mRNAs were reverse-transcribed into double stranded cDNA and subjected to sequencing library construction using the NEBNext Ultra™ II RNA Library Prep Kit for Illumina (NEB, E7770) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Site directed mutagenesis of the selectivity filter region of EhEUKCATB1 was performed using a Q5 Site-Directed Mutagenesis Kit (New England BioLabs, Hitchin, UK). Primer sequences (SDM1_F-TGACCGGAGAcTCCTGGTCTG ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA-seq libraries were prepared using 1 µg of RNA and the NEBNext Ultra™ II RNA Library Prep Kit (NEB, E7775) with rRNA depletion following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2020Quote: ... The PCR products were cloned into the BsmBI-digested optimized sgRNA lentiviral expression vector LRG2.1T using a Gibson assembly kit (New England Biolabs; Cat. No. E2611). The LRG2.1T vector was derived from a lentiviral U6-sgRNA-EFS-GFP expression vector (Addgene ...
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bioRxiv - Cell Biology 2021Quote: ... mutant (GFP-FIP3-I738E-ABD) were engineered from the McCaffrey plasmids GFP-FIP3 and GFP-FIP3I738E using the Q5 Mutagenesis Kit (New England Biolabs, Ipswich, MA) following manufacturer instructions ...
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bioRxiv - Cell Biology 2020Quote: A total of 1 μg of RNA was used for cDNA library construction at Novogene using an NEBNext Ultra 2 RNA Library Prep Kit for Illumina (New England Biolabs; Cat.# E7775) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmid encoding for Spike G614 variant was generated using the pTwist EF1alpha nCoV-2019 S 2xStrep plasmid and the Q5 site-directed mutagenesis kit (New England BioLabs, Evry, France) following the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2020Quote: ... Genomic (g)DNA was extracted from muscle biopsies (from n=27 participants) and isolated using the Monarch kit for DNA isolation (New England Biolabs, Ipswich, MA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Libraries were prepared with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina according to the supplier recommendations (NEB). Briefly ...
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bioRxiv - Developmental Biology 2022Quote: ... RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Immunology 2022Quote: ... The sgRNA templates were generated by PCR (26) and used for synthesis of sgRNAs using NEB HiScribe™ T7 Quick High Yield RNA Synthesis Kit (New England Biolabs, E2050S). The sgRNAs purified using MEGAclear Kit (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... at −80 °C prior to RNA extraction and library preparation (performed by Novogene Company Limited with NEB Next® Ultra™ RNA Library Prep Kit (NEB)) prior to RNA sequencing (paired-end 150 bp ...
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bioRxiv - Cancer Biology 2022Quote: ... The digested constructs were then purified with Ampure XP Beads and then ligated with the Quick Ligation Kit (NEB, Cat. No. M2200S).
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bioRxiv - Genomics 2022Quote: ... NEBNext® UltraTM II Directional RNA Library Prep Kit for Illumina® and NEBNext® UltraTM II DNA Library Prep Kit for Illumina® according to the manufacturer’s instructions (New England Biolabs). Paired-end 100 bp reads were generated.
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bioRxiv - Microbiology 2022Quote: ... Reverse-transcription polymerase chain reaction (RT-PCR) was performed using the Luna Universal Probe One-Step RT-qPCR kit (New England BioLabs; https://www.neb.ca). The 5 ‘ untranslated region (UTR ...
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bioRxiv - Microbiology 2022Quote: Complementary DNA (cDNA) libraries were constructed with the Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA). cDNA concentrations were measured using a Qubit fluorometer with a dsDNA BR/HS kit (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA was transcribed to full-length cDNA using the SMART-Seq253 protocol and RNA-sequencing libraries were generated using the NEBNext® Ultra™ II FS DNA library preparation kit (New England Biolabs) according the manufacturer’s protocol with DNA input below 100 ng ...
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bioRxiv - Neuroscience 2022Quote: The 16S rRNA V4 region was amplified using the following primers 515f-Y: GTGYCAGCMGCCGCGGTA and 806r-N: GGACTACNVGGGTWTCTAAT and The Q5® high-fidelity DNA polymerase kit (New England BioLabs, UK). 2μl of extracted DNA was added to a PCR reaction mix prepared by mixing a final concentration of 1X Q5 reaction buffer ...
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bioRxiv - Immunology 2022Quote: B cells were cultured for 48 hours before total RNA was extracted using Quick-RNA™ Microprep Kit (ZymoResearch) and mRNA was isolated using the NEBNext® Poly(A) mRNA Magnetic Isolation (New England BioLabs). Stranded Illumina libraries were prepared with Swift Rapid RNA Library Kit according to the manufacturer’s instructions (Swift Biosciences) ...