Labshake search
Citations for New England Biolabs :
9601 - 9650 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: Libraries were prepared on an automated liquid handler (Biomek i7) using the NEBNext Ultra II DNA library preparation kit (NEB, E7645), according to the manufacturer’s instructions and without size selection ...
-
bioRxiv - Genomics 2023Quote: ... Paired-end libraries were constructed using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (New England Biolabs, E7645S) using a starting material of 50 ng ...
-
bioRxiv - Molecular Biology 2023Quote: ... with primers listed in Table S8, then inserted into PHR-mCh- CryWT plasmid (Adgene, 101221) by using NEBuilder HiFi DNA Assembly Cloning Kit (NEB, E5520S). The generated constructs were fully sequenced to confirm the absence of any mutations or stop codons ...
-
bioRxiv - Microbiology 2023Quote: ... dual unique indexed libraries for sequencing on all Illumina platforms were made using the NEBNext Ultra™ II Directional RNA Library Prep Kit for Illumina (New England Biolabs). Manufacturer protocol was modified by diluting adapter 1:30 and using 3 μl of this dilution ...
-
bioRxiv - Microbiology 2023Quote: Libraries for RNAseq were prepared using the NEB Next Ultra II Directional RNA Library Prep Kit (New England Biolabs, Ipswich, MA). Individual libraries were uniquely barcoded with NEBNext Multiplex Oligos for Illumina sequencing platform (New England Biolabs ...
-
bioRxiv - Animal Behavior and Cognition 2022Quote: ... Libraries were prepared for both the INPUT and IP RNA using the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina) (NEB, #E6420S) following the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2023Quote: ... Total of 3 μg RNA was prepared for sequencing libraries using the NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA) according to manufacturer’s instructions and sequences attributed to each sample by adding index codes ...
-
bioRxiv - Microbiology 2023Quote: ... The preparation of the sRNA-enriched library was done with NEBNext Ultra II RNA kit (New England Biolabs, Ipswich, MA, USA) following the manufacturer’s recommendation which included cDNA synthesis ...
-
bioRxiv - Neuroscience 2023Quote: ... Ribo-depleted RNA sequencing (RNA-seq) libraries were constructed using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, #E7765S). RNA samples were fragmented ...
-
bioRxiv - Cancer Biology 2023Quote: ... NEBNext Low Input RNA libraries were prepared manually following the manufacturer’s protocol (NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® Instruction Manual Version 5.0_5/20, NEB #E6420L). Samples were normalized to 1ng total RNA material per library in 8μl of nuclease-free water ...
-
bioRxiv - Developmental Biology 2023Quote: ... building parallel total-RNA and poly(A)+ Illumina strand-specific libraries using the Ultra II RNA-seq library kit (NEB #E7765) (see below) ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Linear plasmid fragments were generated by PCR and purified by Monarch® PCR and DNA cleanup kit (New England BioLabs®). Ligations were performed with In-Fusion® enzyme at 50°C for 15 minutes (min ...
-
bioRxiv - Systems Biology 2022Quote: ... A total of 500ng of purified RNA was used as input for the NEBNext Ultra II RNA Library Prep Kit (NEB #E7770S), which first involved enrichment of polyadenylated mRNA using the NEBNext Poly(A ...
-
bioRxiv - Biochemistry 2022Quote: ... were generated from pGB1-MITF81-204 and pGAL4-MITF1-204 using the Q5® QuickChange site-directed mutagenesis kit (New England Biolabs) according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2022Quote: ... 8 µl PCR amplified product was used for the in vitro transcription using the NEB HiScribe T7 High Yield RNA Synthesis kit (New England Biolabs, E2040S), incubating at 37 °C for 18 hours in the thermocycler ...
-
bioRxiv - Neuroscience 2023Quote: ... NEBNext Ultra RNA Library Prep Kit was then used to prepare sequencing libraries according to manufacturer’s instructions (NEB, Ipswich, MA, USA). Oligo(dT ...
-
bioRxiv - Immunology 2023Quote: ... Stranded RNA sequencing libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, cat# E7760L). Purified libraries were qualified on an Agilent Technologies 4150 TapeStation using a D1000 ScreenTape assay (Agilent ...
-
bioRxiv - Molecular Biology 2023Quote: ... Paired-end Illumina sequencing libraries were prepared from CUT&RUN enriched DNA using the NEBNext Ultra™ II DNA Library Prep Kit (New England Biolabs) and sequenced on a NextSeq 500 (Illumina) ...
-
bioRxiv - Molecular Biology 2023Quote: ... cells were harvested by phenol-chloroform extraction and processed according to the NEB Ultra II Directional RNA Sample Prep Kit (New England Biolabs, #E7760S).
-
bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were generated using NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (New England Biolabs, MA, USA). Illumina MiSeq sequencing was carried out on the IlluminaHiseq2500 platform (Illumina Inc. ...
-
bioRxiv - Microbiology 2023Quote: ... RNA was diluted 1:50 for RT-qPCR which was conducted using the Luna Universal One-Step RT-qPCR kit (New England Biolabs E3005E) on a C1000 touch thermal Cycler / CFX384 Real-Time System (Biorad) ...
-
bioRxiv - Microbiology 2022Quote: Genomic DNA was purified from 100μL of CSF samples using the Monarch® Genomic DNA Purification Kit (New England Biolabs, Australia) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... Substrates were transcribed in vitro from purified PCR products using the HiScribe™ T7 Quick High Yield RNA Synthesis Kit (NEB) according to manufacturer’s instructions ...
-
bioRxiv - Genetics 2023Quote: ... strand-specific mRNA libraries were generated using the NEBNext Ultra II Directional RNA library prep Kit for Illumina (New England BioLabs #E7760), and mRNA was isolated using Poly(A ...
-
bioRxiv - Genetics 2023Quote: These three parts were assembled together with the pJmp3 backbone using the NEBuilder HiFi DNA Assembly cloning kit (NEB cat # E5520S) and transformed to DH5α competent cells ...
-
bioRxiv - Microbiology 2023Quote: All parasite materials were centrifuged at 1,000 g for 2 min and total genomic DNA was isolated from the resulting pellets using the Monarch Genomic DNA Purification kit (New England Biolabs, Australia). For the ovine ...
-
bioRxiv - Genetics 2023Quote: ... 100ng of each mRNA sample was used to prepare libraries with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB E7760S) according to the manual ...
-
bioRxiv - Genomics 2023Quote: ... with the exception that elution of the Tn5 treated DNA was performed using the Monarch PCR and DNA clean-up kit (NEB T1030S) following a 5:1 ratio of Binding Buffer:Sample ...
-
bioRxiv - Genomics 2023Quote: ... the samples were incubated with 20-fold diluted Quick Ligase in 1× Quick Ligase Reaction Buffer from Quick Ligation Kit (NEB M2200) supplemented with additional 1 mM ATP (NEB P0756 ...
-
bioRxiv - Biochemistry 2023Quote: ... 0.5 μg of linearized plasmid was used as template in a 10 µL reaction using the HiScribe T7 High Yield RNA Synthesis Kit (NEB # E2040S) with an 8:1 ratio of cap analog to GTP ...
-
bioRxiv - Cell Biology 2023Quote: ... the BRE-Citrine was replaced with CAGA12-mCherry using the NEBuilder® HiFi DNA Assembly Cloning Kit (New England Biolabs, E5520S). For ease of readability these reporters are referred to as BRE-YFP and SBE-RFP in the manuscript.
-
bioRxiv - Developmental Biology 2023Quote: ... A short version of enhD1 luciferase vector was mutated with use of a gBlock (IDT) and NEBuilder HiFi DNA Assembly kit (NEB, E2621). Luciferase reporter vectors were co-transfected with pNL1.1.PGK[Nluc/PGK] (N1441 ...
-
bioRxiv - Molecular Biology 2023Quote: ... 100ng of polyA selected mRNA samples were treated using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB E7760S) according to the manual ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... the pML104-GAL1 vector has a guide sequence 5’- CTCTTAAATTATAGTTGGTT-3’ introduced by Q5® Site-Directed Mutagenesis Kit (New England Biolabs) (Hu et al. ...
-
bioRxiv - Cancer Biology 2023Quote: ... Sequencing libraries were generated with a NEBNext Ultra Directional RNA Library Prep kit according to the supplier’s directions (New England Biolabs, Beverly, MA). Sequencing was performed as previously described on a NovaSeq 600 instrument (Illumina ...
-
bioRxiv - Cancer Biology 2023Quote: ... The U6 promoter was deleted and replaced with an XbaI site using NEB Q5 site directed mutagenesis kit (NEB Cat# E0554S) with primers forward ...
-
bioRxiv - Genetics 2023Quote: ... and inserted to AatII site on pHACK-GAL4>nlsLexA::GADfl (v1) using the HiFi DNA Assembly Cloning Kit (New England BioLabs, E5520S). The resulting construct carrying both 3xP3-RFP and yellow transgene markers ...
-
bioRxiv - Plant Biology 2023Quote: ... The DNA fragment was introduced into pGreen0179_YFP-(AauI)::35St with EXP7a promoter by using a NEBuilder HiFi DNA Assembly kit (NEB, MA, USA), and the resulting construct was transformed into Arabidopsis Col-0 by floral dip (Clough and Bent ...
-
bioRxiv - Genomics 2023Quote: ... Sequencing libraries for genome wide DNA methylation profiles were generated from 200 ng of genomic DNA using NEBNext® Enzymatic Methyl-seq Kit (New England Biolabs). These libraries were sequenced on an Illumina NextSeq 2000 to generate 100 bp paired end reads.
-
bioRxiv - Microbiology 2023Quote: ... AOB and AOA amoA genes were created using the NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs, MA) according to the manufacturer’s guideline ...
-
bioRxiv - Molecular Biology 2023Quote: ... truncations were then constructed from this plasmid by site-directed deletion of the appropriate regions of the SFPQ ORF using the Q5® Site-Directed Mutagenesis Kit (New England Biolabs). The mammalian expression construct encoding GFP-tagged full-length SFPQ was described previously (Huang et al ...
-
bioRxiv - Neuroscience 2023Quote: ... The gRNAs were cloned into a dual gRNA pCFD4 vector having a BbsI restriction site using Gibson Assembly Kit (New England Biolabs (UK) Ltd ...
-
bioRxiv - Microbiology 2023Quote: All clonings were carried on in a Gibson assembly-like reaction with the NEBuilder® HiFi DNA Assembly kit (NEB, E2621) and into plasmids derived from pMM704 or pMM731 (17) ...
-
bioRxiv - Bioengineering 2023Quote: mRNA was synthesized through in vitro transcription using the HiScribe T7 mRNA Kit with CleanCap Reagent AG (NEB, China, Cat: E2080S). The templates for in vitro transcription were linearized plasmids ...
-
bioRxiv - Neuroscience 2023Quote: ... RNA-Seq was performed using libraries prepared with NEBNext Ultra™ Directional RNA Library Prep Kit for Illumina (New England Biolabs) and sequenced on the Illumina NextSeq 500 platform to obtain 36bp paired-end reads ...
-
bioRxiv - Neuroscience 2023Quote: ... ChIP-Seq was performed using libraries prepared with the NEBNext Ultra™ II DNA Library Prep Kit for Illumina (New England Biolabs) as per the manufacturer’s instructions.
-
bioRxiv - Neuroscience 2023Quote: ... we introduced a silent mutation into the PAM motif of the sgRNA located within the 3’ homology arm in the donor plasmid by using Q5 Site-Directed Mutagenesis kit (NEB, E0054). The donor plasmid was confirmed with DNA sequencing ...
-
bioRxiv - Cell Biology 2023Quote: ... RNA was purified by PolyA selection and libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit (NEB, E7770). Samples were sequenced on a NextSeq 2000 (Illumina).
-
bioRxiv - Genetics 2023Quote: RNA was isolated from frozen hippocampal samples stored at -80°C using the Monarch® RNA Total Isolation kit (NEB T2010S). Hippocampus from the left hemisphere was used where possible ...
-
bioRxiv - Molecular Biology 2023Quote: ... The cDNAs were subcloned downstream of a tetracycline response element (TRE) using the Gibson Assembly Cloning kit (New England Biolabs, # E5510S). Mutations were introduced by in vitro mutagenesis using Q5 Site-directed Mutagenesis Kit (New England Biolabs ...