Labshake search
Citations for New England Biolabs :
951 - 1000 of 1706 citations for Urothelial Cell Culture Supplements since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... the cell–ConA bead complexes were incubated with anti- H3K9la (PTM BIOLABS, PTM1419RM) at 4°C overnight and with a secondary antibody for 1 h at room temperature ...
-
bioRxiv - Bioengineering 2022Quote: ... The constructed plasmids were chemically transformed to DH5a competent cells (New England Biolabs) and then their sequences were confirmed through Sanger Sequencing by Sequetech Corporation (Mountain View ...
-
bioRxiv - Biophysics 2022Quote: ... Cells were allowed to recover in SOC medium (NEB 10β Stable Outgrowth Medium) for 30 minutes and later transferred to 200 mL of LB medium with ampicillin 4X overnight ...
-
bioRxiv - Cell Biology 2023Quote: ... and cells were finally treated with 1U/μL Exonuclease III (Cat.# M0206, NEB) in 1X NEBuffer 1 at 37 °C for 15 min in a humid container ...
-
bioRxiv - Biochemistry 2023Quote: ... The plasmids were then transformed into DH5-alpha high-efficiency competent cells (NEB). Following transformation ...
-
bioRxiv - Genomics 2023Quote: ... Cells were allowed to recover in SOC medium (NEB 10β Stable Outgrowth Medium) for 1 hour and later transferred to 100 mL of LB medium with ampicillin overnight ...
-
bioRxiv - Molecular Biology 2023Quote: Both HURP plasmids were transformed into Rosetta2 (DE3) Escherichia coli cells (NEB: C29871) and grown in 2 L of LB media containing 50 μg/mL ampicillin and 25 μg/mL chloramphenicol at 37 °C in a shaking incubator (200 rpm) ...
-
bioRxiv - Cell Biology 2023Quote: ... and then transformed into competent cells (C2987, New England BioLabs, Ipswich, MA, USA). Individual colonies were grown overnight under selection and DNA was extracted by miniprep according to the manufacturer’s instructions but without the presence of RNAse A in the resuspension buffer (FERK0503 ...
-
bioRxiv - Biophysics 2023Quote: ... Cells were allowed to recover in SOC medium (NEB 10β Stable Outgrowth Medium) for 30 minutes and later transferred to LB medium with spectinomycin overnight ...
-
bioRxiv - Biochemistry 2023Quote: ... coli BL21 T7 Express lysY/Iq cells (New England BioLabs, catalog no. C3013) and purified as previously described ...
-
bioRxiv - Bioengineering 2023Quote: ... Cells were washed in ligation buffer (1X T4 DNA Ligase Reaction Buffer (NEB), 0.4 mM ATP ...
-
bioRxiv - Systems Biology 2023Quote: ... Two transformations into NEB 10 beta electrocompetent cells (New England Biolabs, Ipswitch, MA) were performed as described above ...
-
bioRxiv - Cancer Biology 2023Quote: ... RNA was extracted from cells using Monarch Total RNA Miniprep Kit (NEB # T2010S). Any remaining DNA was degraded using DNase I (Thermo Scientific # EN0521) ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... intermedium cells using the Monarch® Total RNA Miniprep Kit (New England Biolabs). For the nutrient medium condition ...
-
bioRxiv - Genomics 2023Quote: ... pHia5ET was transformed into T7 Express lysY/Iq Escherichia coli cells (NEB C3013I). Overnight cultures were added to two 1 L cultures of LB medium supplemented with 50 µg/mL kanamycin and grown with shaking at 37°C to an OD600 of 0.8-1.0 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were stained with γ-H2AX (S139) clone 20E3 (New England BioLabs, UK) or 53BP1 clone 6B3E10 (Santa-Cruz ...
-
bioRxiv - Molecular Biology 2024Quote: Plasmid DNA was purified from Escherichia coli DH5α competent cells (New England Biolabs) using a Qiagen Miniprep kit ...
-
bioRxiv - Cell Biology 2024Quote: ... These plasmids were transformed into E.coli BL21 DE3 competent cell (New England Biolabs) and plated on agar plates containing Ampicillin or Kanamycin ...
-
bioRxiv - Molecular Biology 2024Quote: ... and resuspending cells in 950 uL 10-beta/Stable Outgrowth Medium (NEB, B9035S). Serial dilutions of 10 uL were immediately spread onto Ampicillin selection plates to assay transformed library complexity (>= 10,000x) ...
-
bioRxiv - Microbiology 2024Quote: ... These plasmids (Table S6) in combination with the cell-free system PURExpress (NEB) were used to produce bacteriocins in vitro ...
-
bioRxiv - Cell Biology 2024Quote: ... and A549 Chk2-/- cells using the Monarch® Genomic DNA Purification Kit (NEB). Prior to Sanger sequencing ...
-
bioRxiv - Genomics 2024Quote: ... HiChIP in Ishikawa cells was conducted using restriction enzyme DpnII (New England Biolabs). Crosslinked chromatin was sonicated using an EpiShear probe-in sonicator (Active Motif ...
-
bioRxiv - Biophysics 2021Quote: ... or SNAP-Cell® 505-Star (both from New England Biolabs, Ipswich, MA, USA) to label the SNAP-tagged PEX5L ...
-
bioRxiv - Biophysics 2020Quote: ... was cloned into pET28a and expressed in Escherichia coli ER2566 cells (New England Biolabs, fhuA2 lacZ::T7 gene1 [lon] ompT gal sulA11 R(mcr73::miniTn10--TetS)2 [dcm] R(zgb-210::Tn10--TetS ...
-
bioRxiv - Immunology 2021Quote: ... 100,000 cells were lysed and digested with 300 units of micrococcal nuclease (MNase, NEB) for 15 mins ...
-
bioRxiv - Immunology 2020Quote: ... The proteins were expressed in BL21 DE3 competent cells (New England Biolabs, Ipswich, MA). Protein purification was undertaken using nickel-affinity chromatography (Qiagen ...
-
bioRxiv - Bioengineering 2022Quote: ... Sequence verified plasmids were transformed into BL21(DE3) Escherichia coli cells (New England Biolabs) and plated on LB medium supplemented with 100 μg mL−1 ampicillin ...
-
bioRxiv - Molecular Biology 2021Quote: ... cell lysates were incubated in 40 μg/ml PNGase F (New England Biolabs, MA) for 2 hr at 37 °C to cleave all N-glycans before immunoblotting 62 ...
-
bioRxiv - Genomics 2020Quote: ... Cells from single wells were screened via direct lysis by proteinase K (P8107S; NEB) in 20 μl of single cell lysis buffer (10 mM Tris-HCl pH 7.6 ...
-
bioRxiv - Systems Biology 2019Quote: ... The growth was continuously monitored online using Cell Growth Quantifier (CGQ) (Aquila Biolabs GmBH), and the samples were withdrawn at the start of the experiment ...
-
bioRxiv - Biochemistry 2019Quote: ... dynein-coated beads were labeled with 5 µM SNAP-Cell-TMR (New England Biolabs) in the column for 10 min at room temperature and unbound dye was removed with a 300 mL wash with TEV buffer at 4°C ...
-
bioRxiv - Biophysics 2020Quote: ... The flow cell was then treated with bovine serum albumin (BSA, New England Biolabs) (100 mg/ml ...
-
bioRxiv - Genomics 2019Quote: ... at room temperature for one hour and then transformed into NEB10 competent cells (NEB). Plasmids from independent colonies were isolated using a plasmid DNA minikit and Sanger sequenced to identify correctly inserted clones.
-
bioRxiv - Biophysics 2021Quote: MukF Flag-tagged fragments were expressed from pET 21 plasmids in C3013I cells (NEB).The Flag-tagged MukE and MuF were at the C-terminus ...
-
bioRxiv - Neuroscience 2020Quote: ... cultured HEK cells expressing Endoglycan were treated with O-glycosidase (8’000 U/ml; NEB) or α2-3,6,8 Neuraminidase (5 U/ml ...
-
bioRxiv - Bioengineering 2020Quote: ... Electroporated cells were immediately transferred into pre-warmed SOC recovery media (New England Biolabs) and recovered for 1-2 hours at 37 °C with 250 rpm of shaking ...
-
bioRxiv - Cell Biology 2021Quote: ... dynein-coated beads were labeled with 5 µM SNAP-Cell-TMR (New England Biolabs) in the column for 10 min at room temperature and unbound dye was removed with a 300 mL wash with TEV buffer at 4°C ...
-
bioRxiv - Bioengineering 2021Quote: ... the flow cell was treated with DraI enzyme cocktail (1U DraI enzyme (#R0129, NEB) in 1X CutSmart buffer ...
-
bioRxiv - Biochemistry 2020Quote: ... His-SNAP-EB3 proteins were then coupled to SNAP-Cell 647-SiR dye (NEB) by incubation at 37°C for 30 minutes ...
-
bioRxiv - Biophysics 2020Quote: ... the cells were first labelled with 5 µM biotin-conjugated SNAP-tag substrate (NEB) in tissue culture medium ...
-
Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... SNAP-Tag constructs were labeled with SNAP-Cell® 430 (New England BioLabs, S9109S) or SNAP-Cell® 647-SiR (New England BioLabs ...
-
bioRxiv - Cell Biology 2022Quote: ... dynein-bound beads were mixed with 5 µM SNAP-Cell-TMR (New England Biolabs) for 10 min at RT ...
-
bioRxiv - Biochemistry 2022Quote: ... All other proteins were expressed in Escherichia coli BL21(DE3) cells (New England BioLabs).
-
bioRxiv - Cell Biology 2022Quote: ... in the following abbreviated as BG-ATTO594 and SNAP-Cell 647-SiR (NEB, # S9102S), in the following abbreviated as BG-SiR ...
-
bioRxiv - Cancer Biology 2020Quote: Specific antibodies against Slug (C19G7, Cell Signaling Technology, NEB, Frankfurt, Germany, #9585, 1:400), pan-cytokeratine (polyclonal ...
-
bioRxiv - Bioengineering 2021Quote: ... coli cell libraries using M13K07 helper phage (New England Biolabs, Whitby, Canada, Cat#N0315S) as described in 81 and used for selection experiments described below.
-
bioRxiv - Cell Biology 2019Quote: ... S149A-(A54T) and S149E-(S99P) expression vectors were transformed into T7 express cells (NEB) and proteins were produced as described previously (Schulte et al. ...
-
bioRxiv - Biophysics 2019Quote: ... The flow cell was then treated with bovine serum albumin (BSA, New England Biolabs) (100 mg/ml ...
-
bioRxiv - Cell Biology 2019Quote: ... Cells were labeled for 15 min with 2 μM TMR-Star (New England Biolabs) in complete medium for pulse labeling ...
-
bioRxiv - Plant Biology 2019Quote: ... and 300mL of the cell lysate was mixed with amylose resin (New England Biolabs) for the MBP-tagged proteins or glutathione sepharose resin (GE Healthcare Life Sciences ...