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Citations for New England Biolabs :
851 - 900 of 1706 citations for Urothelial Cell Culture Supplements since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... and transformed them into chemically competent NEB-5α cells (New England Biolabs) according to manufacturers’ instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 μL of ligation product was then transformed into competent cells (NEB). Transformation and plasmid DNA recovery were performed as previously described ...
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bioRxiv - Molecular Biology 2022Quote: ... Correct constructs were re-transformed into 10-beta chemically competent cells (NEB), plated on LB agar plates containing chloramphenicol ...
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bioRxiv - Genetics 2022Quote: ... The ligation mix was transformed using NEB stable competent cells (NEB, C3040). Correct insertion of sgRNA sequence was confirmed by Sanger sequencing.
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bioRxiv - Cell Biology 2023Quote: ... After 24 h cell lysates were treated with PNGase-F (NEB, P0704L) or water by following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... ligated with the cut backbone and transformed into competent cells (NEB, C2987H). EGFP-YgiM(TA ...
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bioRxiv - Cell Biology 2023Quote: ... The obtained yeast plasmid DNA was transformed into competent cells (NEB, C2987H). Yeast strains ...
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bioRxiv - Immunology 2023Quote: ... We then electroporated NEB 10beta cells (New England BioLabs, Cat. No. C3020K) following the protocol (http://www.neb.com/protocols/0001/01/01/electroporation-protocol-c3020 ...
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bioRxiv - Cell Biology 2023Quote: ... The ligated plasmid was transformed into Stable competent cells (New England Biolabs), and individual clones were picked and sequenced (Laragen ...
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bioRxiv - Biochemistry 2023Quote: ... Mutagenesis reactions were transformed into T7 express bacterial cells (New England Biolabs) and plated on LB-Amp agar plates ...
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bioRxiv - Molecular Biology 2023Quote: ... E.coli T7 express competent cells (C2566 New England Biolabs Inc., Ipswich, MA) were transformed with pLB047 ...
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bioRxiv - Biochemistry 2023Quote: NEBExpress Iq chemically competent cells (New England Biolabs, USA; cat. no. C3037I)
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bioRxiv - Biochemistry 2023Quote: ... and SNAP tags were stained with SNAP-Cell® 505-Star (NEB). HaloTag® Ligands TMRDirect were added to medium to a final concentration of 50nM and incubated overnight ...
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bioRxiv - Cancer Biology 2023Quote: RNA was harvested from 1 million cells using Bio-TRI® (BioLabs) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was harvested from the treated cells using Bio-TRI® (BioLabs) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... coli competent cells (NEB 5-alpha, New England Biolabs, Ipswich, MA, USA) that were cultured and prepared using a GenElute HP Plasmid Midi kit (NA0200-1KT ...
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bioRxiv - Cancer Biology 2022Quote: ... Ligated plasmids were transformed into NEB stable cells (New England Biolabs #C3040) following manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... coli σ70 (σ70) was overexpressed in T7 Express cells (New England Biolabs) as inclusion bodies from the pET-28a-σ70 overexpression plasmid39 by growing the cells at 37oC to an OD600 of ∼ 0.8 ...
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bioRxiv - Cell Biology 2023Quote: ... Cell pellets were lysed and subsequently incubated with λ-protein phosphatase (NEB) for 1 h at 30°C according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... and the resultant plasmids were transformed in E.Coli BL21-DE3 cells (NEB).
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bioRxiv - Cell Biology 2023Quote: ... The amplified product was transformed in Escherichia coli NEB5α cells (NEB#C2987) to obtain colonies ...
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bioRxiv - Bioengineering 2023Quote: ... The cells were grown in 1 mL SOC Outgrowth Medium (B9020, NEB) for 2 h in a standard glass tube at 30 °C with 160 rpm ...
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bioRxiv - Neuroscience 2023Quote: ... ligation products were cloned in NEB Stable competent cells (New England Biolabs), which were grown at 30°C on LB plates ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were harvested 30 hours after transfection using Passive Lysis Buffer (NEB) and luciferase activity determined with Bright-Glo luciferin reagent (Promega) ...
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bioRxiv - Molecular Biology 2023Quote: ... and then transformed into NEB 10-beta competent cells (New England BioLabs). Plasmid DNA was extracted from ten positive clones using the GeneJET Plasmid Miniprep Kit (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... The cleared cell lysate was applied to Amylose resin (New England Biolabs), followed by washing with buffer A and elution with buffer A containing 10 mM maltose ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli cells were used (New England Biolabs GmbH, Germany, Art. No. C2987) for the cloning of certain plasmids ...
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bioRxiv - Developmental Biology 2024Quote: Single oocytes were collected with NEBNext Single Cell Lysis Module (NEB #E5530S) in 8μl volume and flash-frozen and stored at −80°C ...
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bioRxiv - Biochemistry 2024Quote: ... The Assembly reactions were transformed into DH-10b electro-competent cell (NEB) and split into 2% and 98% before spreading on Agar plates containing 50ng/ul Kanamycin ...
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bioRxiv - Microbiology 2023Quote: ... the product was transformed into DH5-α cells (NEB, Cat. No. C2987H). Following culture on LB-ampicillin plates (Invitrogen ...
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bioRxiv - Synthetic Biology 2024Quote: ... The resulting helper plasmids were transformed into DH5α competent cells (NEB, C2987H), yielding four individual helper cell lines (M13cp-dg1 ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were treated with 0.24mg/ml Monarch RNase A (New England Biolabs) for 1 hour at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... and cells were resuspended in 1 X DNAse buffer (New England Biolabs) and then treated with DNAse (New England Biolabs ...
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bioRxiv - Biochemistry 2024Quote: ... Correct constructs were re-transformed into 10-beta chemically competent cells (NEB), from which clones were used to prepare glycerol stocks (25% glycerol ...
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bioRxiv - Cell Biology 2024Quote: ... Transfected cells were labeled with 1 µM SNAP-Surface 674 dye (NEB) for 30min followed by washing two times for 10 min with 300 µl MES300 per well ...
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bioRxiv - Biophysics 2021Quote: ... This vector was transformed into T7 express cells (enhanced BL21 derivative, NEB C2566I), grown to OD∼0.4-0.8 ...
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bioRxiv - Biophysics 2021Quote: ... Cells were allowed to recover in SOC medium (NEB 10β Stable Outgrowth Medium) for 30 minutes and later transferred to 200 mL of LB medium with ampicillin 4X overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... the ligated products were transformed into NEB 5α competent cells (New England BioLabs) using the heat shock method ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then transfected with sgRNA and the EnGen Cas9 NLS protein (NEB) using Lipofectamine RNAiMAX and according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... washed before either incubation with SNAP-Cell TMR-Star at 2 μM (NEB) and SNAP-Surface Block at 13 μM (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... Primary antibodies used were from Cell Signalling Technologies (New England BioLabs, ON, CAN). They included phosphorylated and total extracellular signal-regulated kinase (P-ERK 1/2 ...
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bioRxiv - Genomics 2019Quote: ... Cells were resuspended in 1.2 mL of 1X DpnII buffer (NEB, Ipswich, MA), transferred to a VK05 tubes (Precellys ...
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bioRxiv - Genomics 2020Quote: ... and the resulting product was transformed into 10-Beta Electrocompetent cells (NEB C3020K). We plated 1% of the library to estimate complexity and grew the rest of the sample and then midi prepped (Zymo D4200 ...
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bioRxiv - Neuroscience 2019Quote: ... was first transformed into NEB 5-alpha competent cells (New England Biolabs #E2621S) and plated on LB plus ampicillin (60 µl/ml).
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Differential turnover of Nup188 controls its levels at centrosomes and role in centriole duplicationbioRxiv - Cell Biology 2019Quote: ... we used either a 647-SiR dye (SNAP-Cell® 647-SiR, NEB) or TMR-Star dye (SNAP-Cell® TMR-Star ...
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bioRxiv - Systems Biology 2020Quote: ... cells were then resuspended in 600 μL 1x T4 ligase buffer (M0202L, NEB). The following additional reagents were added to make a master mix ...
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bioRxiv - Immunology 2019Quote: ... Gel filtered proteins were labeled with either SNAP-Cell 505 (NEB, catalog S9103S) or SNAP-Cell TMR (NEB ...
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bioRxiv - Microbiology 2019Quote: ... For live cell imaging parasites were stained with Hoechst 33342 (New England Biolabs), transferred to poly-L-lysine-coated μ-slides VI (Ibidi ...
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bioRxiv - Cell Biology 2020Quote: ... 50 µg cell lysate was treated with 80 units of lambda-phosphatase (NEB) at 30°C for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... anti-glutaryl-lysine (Cell Signaling Technologies, non-commercial #14943MF and PTM-Biolabs #1151), anti-succinyl-lysine (Cell Signaling Technologies ...