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Citations for New England Biolabs :
901 - 950 of 1706 citations for Urothelial Cell Culture Supplements since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... anti-succinyl-lysine (Cell Signaling Technologies, non-commercial # 13599 and PTM-Biolabs #401), anti-malonyl-lysine (Cell Signaling Technologies ...
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bioRxiv - Biochemistry 2021Quote: Plasmid propagation and subcloning was performed using Escherichia coli DH10β competent cells (NEB). The codon optimized GTL gBlock (Supporting Information Table 1 ...
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bioRxiv - Microbiology 2021Quote: ... cells were lysed in 150μL of 1x Gel Loading Buffer (New England Biolabs), sonicated and boiled 5min at 95°C ...
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bioRxiv - Biophysics 2021Quote: ... where GRN-3 was expressed in Escherichia coli SHuffle cells (New England Biolabs) while other GRNs were expressed in Origami 2 DE3 cells (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... The vector was transformed into BL21 (DE3) competent cells (New England Biolabs, UK) for expression ...
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bioRxiv - Synthetic Biology 2021Quote: Entry and expression plasmids were grown in NEB-5α cells (New England Biolabs). Destination plasmids were grown in One Shot ccdB survival cells (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... The assembly reaction was then transformed into high efficiency DH5alpha cells (NEB C2987I). For eutD ...
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bioRxiv - Synthetic Biology 2022Quote: ... These plasmids were transformed into chemically competent BL21(DE3) cells (New England Biolabs) and single transformants selected for using ampicillin resistance.
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bioRxiv - Biochemistry 2022Quote: Cell lines and molecular biology materials were purchased from New England Biolabs (NEB) unless otherwise indicated ...
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bioRxiv - Cancer Biology 2022Quote: ... The plasmid was maintained in T7 Express lysY/Ig Competent E.coli cells (NEB). To prepare the transposase ...
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The Batten disease protein CLN3 is important for stress granules dynamics and translational activitybioRxiv - Molecular Biology 2022Quote: ... cells were lysed in 150μL of 1x Gel Loading Buffer (New England Biolabs), sonicated and boiled 5min at 95°C ...
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bioRxiv - Microbiology 2022Quote: ... All cloning and strains were performed in dh10b genotype cells (NEB, Intact Genomics).
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bioRxiv - Cell Biology 2022Quote: ... Cells were labelled in full media with SNAP-Surface 549 (New England Biolabs) for 30 minutes at 37°C and imaged in RPMI without phenol red in a Nikon Eclipse Ti spinning disk confocal microscope with an ORCA-Flash 4.0 camera (Hamamatsu ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were preserved in a 1:3 glacial acetic acid: methanol (Biolabs-chemicals) solution and karyotyped using g-banding.
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bioRxiv - Plant Biology 2021Quote: ... constructs were separately introduced into the Escherichia coli (Express Competent cells C2523H, NEB). The purification was performed using amylose resin (NEB ...
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bioRxiv - Developmental Biology 2021Quote: ... and injected into 1-cell stage zebrafish embryos together with I-SceI (NEB). Only embryos exhibiting low mosaic sfGFP expression were grown to adulthood ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were labelled with 1 μM SNAP-Surface 549 (New England Biolabs) for 15 minutes at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... and E-cadherin (24E10, Cell Signaling Technology, NEB, Frankfurt, Germany, #3195, 1:400) were used for immunohistochemistry (IHC ...
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bioRxiv - Biochemistry 2021Quote: ... Transformation of the resulting construct into NiCo21(DE3) cells (New England Biolabs, USA) and culture in a ZY5052 medium (25 ...
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bioRxiv - Cancer Biology 2021Quote: ... The plasmid was amplified in DH5a cells (NewEngland Biolabs, Ipswich, MA; Cat #C2987H), and purified using plasmid purification midi-kit (Qiagen ...
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bioRxiv - Molecular Biology 2022Quote: ... The ligation was directly transformed into NEB-5-alpha electrocompetent cells (NEB C2987H) and plated on LB-agar supplemented with carbenicillin (100 µg/mL) ...
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bioRxiv - Microbiology 2022Quote: Total RNA was extracted from cells using Monarch total RNA Miniprep kit (NEB). cDNA synthesis was performed using LunascriptTM RT Supermix Kit (NEB ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were stained with SNAP-Surface® 549 (DY549P1) (S9112S, New England Biolabs) for 15 min in a 37° incubator with 5%CO2 and sorted for the fluorescent signal.
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bioRxiv - Cancer Biology 2019Quote: ... cells were incubated with uracil DNA glycosylase (UDG) to remove uracil (NEB #M0304S), formamidopyrimidine [Fapy]-DNA glycosylase (Fapy-DNA glycosylase NEB #M0240S ...
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bioRxiv - Molecular Biology 2019Quote: ... A total of 107 cells were cross-linked and digested with HindIII (NEB). After inactivating HindIII ...
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bioRxiv - Cell Biology 2019Quote: ... The time taken for each cell to progress from nuclear envelope breakdown (NEB) to anaphase onset (chromatid separation ...
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bioRxiv - Microbiology 2019Quote: ... Growth was monitored every 2 min using a Cell Growth Quantifier (Aquila Biolabs).
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bioRxiv - Genetics 2019Quote: ... Cells were incubated with 300 μL of streptavidin magnetic beads (New England Biolabs) and manually washed 4 times with PBS on a magnetic stand ...
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bioRxiv - Genomics 2021Quote: ... each cell pellet was resuspended in 500 uL of Nuclear Extraction Buffer (NEB, 20 mM HEPES pH 7.9 ...
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bioRxiv - Cell Biology 2021Quote: ... containing 2□ μl of 1× NEB Next Cell Lysis Buffer (New England Biolabs). FACS sorting was performed with a BD Influx sorter (BD Biosciences ...
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bioRxiv - Physiology 2020Quote: ... and transformed into sub-cloning efficiency competent cells (New England Biolabs, Ipswich, MA). All constructs were sequenced to confirm successful generation of the expected mutation (Eurofins Genomics ...
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bioRxiv - Cell Biology 2019Quote: ... Restriction enzymes and competent cells were from New England Biolabs (NEB, Ipswich, MA). Oligonucleotides used for plasmid generation were synthesized at Integrated DNA Technologies (Coralville ...
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bioRxiv - Biochemistry 2021Quote: ... as described by manufacturer and transformed into heat- competent 10-Beta cells (NEB). Inserts from single colonies were PCR amplified (using primers pGEM_T7_Fo ...
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bioRxiv - Microbiology 2021Quote: ... Sequence-verified plasmids were transformed into chemically competent NiCo21(DE3) cells (NEB, #C2529H) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: All proteins were expressed in Escherichia coli NiCo21 (DE3) cells (New England BioLabs). To co-express multiple proteins ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The resulting plasmid lentiMPRA library was electroporated into 10-beta competent cells (NEB) using a Gemini X2 electroporation system (BTX ...
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bioRxiv - Molecular Biology 2022Quote: ... pHia5ET was transformed into T7 Express lysY/Iq Escherichia coli cells (NEB C3013I). Overnight cultures were added to two 1 L cultures of LB medium supplemented with 50 µg/mL kanamycin and grown with shaking at 37°C to an OD600 of 0.8-1.0 ...
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bioRxiv - Biochemistry 2022Quote: ... coli cells following the recommended High Efficiency Transformation Protocol (C2987, New England Biolabs) described by the commercial strain provider ...
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bioRxiv - Biochemistry 2022Quote: ... Cell lysates were applied to a column containing amylose resin (New England Biolabs). The beads were washed with at least ten column volumes of buffer containing 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Genomics 2022Quote: ... Permeabilized cells were resuspended in 300µl 1.25 x DpnII buffer (New England Biolabs) plus 0.3% SDS and incubated for 1 hr at 37°C ...
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bioRxiv - Genomics 2022Quote: ... ligated overnight at 16°C and subsequently transformed into competent cells (NEB C2992H).
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bioRxiv - Microbiology 2022Quote: ... cell DNA was extracted using the Monarch genomic DNA purification kit (NEB #T3010L) or the NucleoSpin Tissue kit (Macherey-Nagel #740952-250 ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids (Table S3) were propagated in Escherichia coli DH5α cells (NEB, United Kingdom) by growing cells in LB media without NaCl (Formedium ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli cells were subjected to high fidelity Q5 Polymerase (NEB, Singapore; Cat: M0491L) PCR to generate 200 bp templates of the Ag43 signal peptide (Figure 1A) ...
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bioRxiv - Cell Biology 2023Quote: HeLa cell lysates were incubated with PNGase F (New England Biolabs, NEB-P0704S) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: HeLa cell lysates were incubated with PNGase F (New England Biolabs, NEB-P0704S) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The reaction was transformed into chemically competent NEB Stable cells (NEB Cat#C3040H) and the plasmid was verified by restriction enzyme digestion and Sanger sequencing ...
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bioRxiv - Neuroscience 2023Quote: ... In-Fusion reactions were transformed into NEB Stable competent cells (New England Biolabs). Insert-containing clones were subjected to restriction digest analysis and sequence verification prior to subsequent cloning steps or packaging into viral particles ...
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bioRxiv - Molecular Biology 2023Quote: ... T7 express or NiCo21 competent cells (C2529 New England Biolabs Inc., Ipswich, MA) were transformed with each clone ...
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bioRxiv - Molecular Biology 2023Quote: ... The golden-gate products were transformed into electrocompetent E.coli DH10β competent cells (NEB) using 0.1 mm electroporation cuvettes (Bio-Rad ...